FastQCFastQC Report
Tue 24 May 2016
ERR841026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147486
Sequences flagged as poor quality0
Sequence length24-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA147761.2876845556285654No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA141531.2333919542373502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA135381.1797965291079804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA112960.9844128817257901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA111430.9710793857179956No Hit
AAAAAAAAAAAAAAAAAAAAAAAA87510.762623683426203No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT84840.7393554256871108No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT84020.7322093690031948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68340.5955628216814846No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC54230.4725983584984915No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA47250.4117697296524751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38750.3376947518313949No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC37410.32601704944548343No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG35230.30701899631019464No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA34880.3039688501646207No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA33560.29246544184417067No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA32880.28653944361848427No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA32620.28427362076748647No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA28770.2507220131661737No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26240.22867381388531105No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA23690.20645132053898696No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA23050.20087391044422329No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT22580.1967779999058812No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC21080.18370594499627882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19610.1708953311848685No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC18010.15695180594795927No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17640.15372736573692403No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA17040.14849854377308305No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA16590.14457692730020236No Hit
TTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG16590.14457692730020236No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG15770.1374308706162864No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC15680.13664654732171025No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA15280.13316066601248294No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC13650.11895569967738169No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13550.11808422935007486No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA13460.11729990605549871No Hit
TAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGTGTATGATATGT13250.11546981836815437No Hit
TAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA13240.1153826713354237No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG12990.11320399551715665No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT12820.11172249596073504No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG12800.11154820189527366No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC12600.10980526124066002No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT12520.10910808497881455No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC12290.10710370322600886No Hit
TAAAAAAAAAAAAAAAAAAAAAAA11920.10387926301497359No Hit
CCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAAC11770.10257205752401337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGAC203.669429E-7206.3382744
CGGATTA153.0109832E-5206.3382744
GTCCCCC100.0024669187206.3382744
GGTTCAC100.0024669187206.3382744
GCCAGCG150.008295682137.5588444
CGGTTCA100.009518959131.3829843
GCAACGC252.3053723E-4123.80295644
AACGACA750.0122.62411543
CTTTAGA355.9502727E-6117.9075744
GCTAATA355.9502727E-6117.9075744
CGTTCTA407.2759576E-11114.9601143
ACGAACA353.768946E-9112.6139943
GCACATA1100.0112.5481444
AGGTCGA659.458745E-11111.10522544
TACGGTG301.9805884E-7109.48582543
CATGACC4000.0108.3275944
GCGATAT251.0543328E-5105.10638443
TGCACCG251.0543328E-5105.10638443
ACGGTGT251.0543328E-5105.10638443
GCTTCGT304.7619556E-4103.16913644
Produced by FastQC (version 0.11.5)