##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841026.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1147486
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.51734661686504	38.0	35.0	39.0	29.0	39.0
2	36.68536871038078	39.0	35.0	39.0	33.0	39.0
3	36.97698185424485	39.0	35.0	39.0	33.0	39.0
4	37.40682587848566	39.0	35.0	39.0	34.0	40.0
5	38.173349391626566	40.0	36.0	41.0	34.0	41.0
6	38.00497696703925	40.0	36.0	41.0	34.0	41.0
7	38.11937836278612	40.0	36.0	41.0	34.0	41.0
8	38.019707429981715	39.0	36.0	41.0	34.0	41.0
9	38.01630956717555	39.0	36.0	41.0	34.0	41.0
10	37.986635131060424	40.0	36.0	41.0	34.0	41.0
11	37.95110964316776	39.0	36.0	41.0	34.0	41.0
12	37.94977629356698	39.0	36.0	41.0	34.0	41.0
13	37.782445276020795	39.0	36.0	40.0	34.0	41.0
14	37.54549510843705	38.0	35.0	40.0	34.0	41.0
15	37.210722396613114	38.0	35.0	40.0	33.0	41.0
16	37.16783298445471	38.0	35.0	40.0	33.0	41.0
17	37.22959408655095	38.0	35.0	40.0	33.0	41.0
18	37.149137331523	38.0	35.0	40.0	33.0	41.0
19	37.241765041142116	38.0	35.0	40.0	33.0	41.0
20	37.126771917042994	38.0	35.0	40.0	33.0	41.0
21	37.018099567227836	38.0	35.0	40.0	33.0	41.0
22	36.91225601009511	38.0	35.0	40.0	33.0	41.0
23	36.918104447461666	38.0	35.0	40.0	33.0	41.0
24	36.62269169297055	38.0	35.0	40.0	33.0	41.0
25	36.61585371245064	38.0	35.0	40.0	32.0	41.0
26	36.42233257638743	38.0	35.0	40.0	32.0	41.0
27	36.27579108621762	38.0	35.0	40.0	31.0	41.0
28	36.26894498582264	38.0	35.0	40.0	31.0	41.0
29	36.228947661550826	38.0	34.0	40.0	31.0	41.0
30	36.38209690453743	38.0	35.0	40.0	31.0	41.0
31	36.41094396704192	38.0	35.0	40.0	31.0	41.0
32	36.32152372465117	37.0	34.0	40.0	31.0	41.0
33	36.22019035330947	37.0	34.0	40.0	31.0	41.0
34	36.210748010930885	37.0	34.0	40.0	31.0	41.0
35	36.04477640200409	37.0	34.0	40.0	31.0	41.0
36	36.16021190368977	37.0	34.0	40.0	31.0	41.0
37	35.94599009789989	36.0	34.0	39.0	31.0	41.0
38	35.84325555339311	36.0	34.0	39.0	31.0	41.0
39	35.76041619263699	36.0	34.0	39.0	31.0	41.0
40	35.64157198367026	36.0	34.0	39.0	30.0	41.0
41	35.4072994185179	36.0	34.0	39.0	30.0	41.0
42	35.32058564479497	35.0	34.0	39.0	30.0	41.0
43	35.09425252704983	35.0	33.0	39.0	29.0	40.0
44	34.91896779522015	35.0	33.0	39.0	29.0	40.0
45	34.63317905125086	35.0	33.0	38.0	28.0	40.0
46	34.37510316046464	35.0	32.0	38.0	28.0	40.0
47	34.27010957529188	35.0	32.0	37.0	28.0	40.0
48	35.098578900309384	35.0	34.0	38.0	30.0	40.0
49	35.7586356273204	36.0	34.0	39.0	31.0	40.0
50	35.76005285009572	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	18.0
22	82.0
23	162.0
24	338.0
25	643.0
26	1278.0
27	2375.0
28	4637.0
29	9202.0
30	17131.0
31	31036.0
32	53177.0
33	84889.0
34	117257.0
35	95354.0
36	162598.0
37	190409.0
38	150143.0
39	146984.0
40	79767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.166724474198375	20.258199228574465	28.575076297227152
2	19.88067828278515	34.61218698964519	28.444355748131134	17.062778979438527
3	20.453408581891193	31.709057888287962	22.736835133500538	25.100698396320304
4	17.284393883672657	36.0187400979184	24.620343951908783	22.076522066500157
5	16.211875351856143	37.42276594224243	23.462944210212587	22.902414495688834
6	16.440897753872378	40.15395394802203	22.817446138776422	20.587702159329176
7	19.81191927396064	38.07340568860971	19.667778081824093	22.44689695560556
8	20.10848062634315	36.67277857856217	23.58599582042831	19.63274497466636
9	18.744891005206163	37.99540909431575	24.604308897886337	18.655391002591752
10	22.652302511751778	32.79412559281769	23.999944225899053	20.55362766953148
11	19.848956762871182	36.29090028113633	21.559042986145364	22.301099969847126
12	18.65652391401725	35.413329661538356	23.866173530657456	22.06397289378694
13	18.47377658638101	35.125657306494375	23.923429131161512	22.477136975963106
14	16.203073501550346	36.839839440306896	22.72036434431444	24.236722713828318
15	18.837964036162532	40.70446175378174	23.497192994075743	16.96038121597998
16	16.011611470641036	43.8411449028572	20.57166710530673	19.57557652119503
17	18.126757102047435	45.10852419985952	17.64335251149034	19.12136618660271
18	18.09982866893365	37.62686429289769	21.486275213815244	22.787031824353413
19	20.264212373832883	34.65584765304326	21.70919732354033	23.370742649583523
20	17.819476664639044	34.529920190747426	25.06610102432622	22.584502120287304
21	16.596019472132994	40.06846270891322	22.218833170949363	21.11668464800442
22	20.626134000763408	35.946146619653746	22.240794223197494	21.18692515638535
23	21.81490667424265	35.47982284751187	22.23268954915354	20.472580929091947
24	17.579822324629667	37.649696815473135	24.171100998182112	20.59937986171509
25	19.828767668336607	35.3932614073837	21.466200049868345	23.31177087441135
26	18.858491951181943	39.32737805169151	22.7016212934497	19.11250870367685
27	20.787440574170432	36.335005173867735	21.24296188277231	21.634592369189527
28	20.395568963108673	32.03940587255861	22.766925043136162	24.798100121196562
29	19.930163742293555	33.23188833947589	24.78063569397965	22.057312224250907
30	22.96966731898239	32.42144481329773	24.701506136257397	19.907381731462483
31	22.900338711124054	30.87955045445338	23.504496160904413	22.71561467351815
32	19.35754529651087	32.43218936797967	26.491649948850128	21.718615386659334
33	18.720315449716413	29.80881960684577	29.23110165478768	22.23976328865014
34	20.574445036424127	33.06197262690579	25.37458933581617	20.988993000853913
35	18.784587921826695	29.62340781165026	26.13424189797161	25.457762368551435
36	19.801128387148044	30.02887187773157	25.896855879002995	24.273143856117393
37	18.68693070131512	33.87609455363139	22.114868205118896	25.32210653993459
38	20.992519491579188	28.091421984759418	23.888165485147045	27.02789303851435
39	19.024679880988757	31.67525879311503	26.74990086007944	22.55016046581677
40	18.19880969947371	29.474748468403476	28.127599342000998	24.198842490121816
41	19.293799329543734	32.264353854128785	24.12979396677782	24.31205284954966
42	19.113485165513172	34.324534578035305	24.63550887923172	21.92647137721981
43	19.100858726609417	27.798058146796123	25.275031648331225	27.826051478263235
44	19.138197631998068	28.40488726645068	24.676334016566965	27.78058108498429
45	18.154171123387318	28.151258447372353	31.43279183667475	22.26177859256558
46	18.429453397647595	31.742423303036187	26.8312274131971	22.996895886119116
47	19.621355383546756	31.712192868466442	27.28547736873066	21.380974379256138
48	19.006219289291902	29.87039191017637	25.571288405693892	25.55210039483783
49	16.034216089737917	32.2038810368549	25.900628128822845	25.861274744584335
50	19.82264356564551	29.46151731853274	26.195516421624177	24.52032269419758
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127556.0
1	121914.5
2	71660.0
3	26223.5
4	24119.0
5	17553.0
6	8392.0
7	4420.0
8	3849.0
9	3033.5
10	2561.5
11	2438.0
12	2410.5
13	2294.5
14	2181.0
15	2270.0
16	2506.5
17	2738.0
18	3125.0
19	3878.5
20	4707.0
21	5331.0
22	6025.5
23	7173.5
24	8444.5
25	9878.0
26	11913.5
27	14928.5
28	17837.0
29	19664.5
30	22160.0
31	27048.5
32	35621.5
33	40559.5
34	42767.0
35	49223.0
36	52798.0
37	52522.0
38	53268.5
39	56450.5
40	62434.0
41	69532.5
42	74325.5
43	74915.0
44	73374.5
45	78337.0
46	85110.0
47	82024.5
48	81245.5
49	88145.0
50	96515.0
51	100688.5
52	94699.5
53	79896.5
54	67267.5
55	62286.0
56	58459.0
57	52308.0
58	45870.5
59	39850.5
60	32803.0
61	25841.0
62	20298.5
63	16172.0
64	12573.5
65	9854.5
66	6991.0
67	4447.0
68	2949.5
69	2118.5
70	1603.0
71	1180.5
72	818.5
73	595.5
74	500.5
75	441.5
76	406.5
77	321.5
78	199.0
79	85.5
80	23.0
81	14.5
82	10.5
83	9.0
84	9.0
85	7.0
86	4.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	16508.0
25	20815.0
26	23935.0
27	26792.0
28	27021.0
29	22679.0
30	17445.0
31	13794.0
32	11243.0
33	8137.0
34	8460.0
35	6832.0
36	7256.0
37	6552.0
38	7079.0
39	8043.0
40	10138.0
41	10085.0
42	12319.0
43	20276.0
44	44959.0
45	91307.0
46	124033.0
47	96254.0
48	177725.0
49	120420.0
50	207379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.142884716888611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0234183361763	8.381434116217646
2	11.030847165491405	2.6789261092035925
3	4.319726439995977	1.573618205081004
4	2.1701279475280097	1.0540645395092287
5	1.4278958757900286	0.8669387503719509
6	1.0259411265958087	0.7474730895940638
7	0.7651362267333961	0.6503672695757142
8	0.6288240020496185	0.6108589891280837
9	0.48391634396308114	0.5288526339625719
>10	5.448208499249199	15.957387733402955
>50	2.267924270866456	19.680709480637713
>100	1.3112591468364687	25.773946696365424
>500	0.05466657851599926	4.443848871270723
>1k	0.03472066473313466	7.829997303672008
>5k	0.0036936877375675177	3.3992452235195056
>10k+	0.0036936877375675177	5.822330988487809
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	14776	1.2876845556285654	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	14153	1.2333919542373502	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	13538	1.1797965291079804	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11296	0.9844128817257901	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	11143	0.9710793857179956	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	8751	0.762623683426203	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8484	0.7393554256871108	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8402	0.7322093690031948	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6834	0.5955628216814846	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5423	0.4725983584984915	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4725	0.4117697296524751	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3875	0.3376947518313949	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	3741	0.32601704944548343	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	3523	0.30701899631019464	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3488	0.3039688501646207	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3356	0.29246544184417067	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3288	0.28653944361848427	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	3262	0.28427362076748647	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	2877	0.2507220131661737	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2624	0.22867381388531105	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2369	0.20645132053898696	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	2305	0.20087391044422329	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	2258	0.1967779999058812	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2108	0.18370594499627882	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1961	0.1708953311848685	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	1801	0.15695180594795927	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1764	0.15372736573692403	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1704	0.14849854377308305	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	1659	0.14457692730020236	No Hit
TTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG	1659	0.14457692730020236	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1577	0.1374308706162864	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1568	0.13664654732171025	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1528	0.13316066601248294	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1365	0.11895569967738169	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1355	0.11808422935007486	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1346	0.11729990605549871	No Hit
TAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGTGTATGATATGT	1325	0.11546981836815437	No Hit
TAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA	1324	0.1153826713354237	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG	1299	0.11320399551715665	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	1282	0.11172249596073504	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1280	0.11154820189527366	No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC	1260	0.10980526124066002	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1252	0.10910808497881455	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1229	0.10710370322600886	No Hit
TAAAAAAAAAAAAAAAAAAAAAAA	1192	0.10387926301497359	No Hit
CCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAAC	1177	0.10257205752401337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1868432381745834	0.0	0.0	0.0	0.0
2	0.3537298058538405	0.0	0.0	0.0	0.0
3	0.4695482123529176	0.0	0.0	0.0	0.0
4	0.5577410094763683	0.0	0.0	0.0	0.0
5	0.627545782693645	0.0	0.0	0.0	0.0
6	0.7207059606827447	0.0	0.0	0.0	0.0
7	0.7997483193694738	0.0	0.0	0.0	0.0
8	0.8769605903688585	0.0	0.0	0.0	0.0
9	0.950686980059016	0.0	0.0	0.0	0.0
10	1.034958160709586	0.0	0.0	0.0	0.0
11	1.117573547738273	0.0	0.0	0.0	0.0
12	1.2031519338798033	0.0	0.0	0.0	0.0
13	1.2816714103701483	0.0	0.0	0.0	0.0
14	1.3463345086563148	0.0	0.0	0.0	0.0
15	1.3963569054437266	0.0	0.0	0.0	0.0
16	1.4435034501510258	0.0	0.0	0.0	0.0
17	1.479408027636067	0.0	0.0	0.0	0.0
18	1.5166198106120685	0.0	0.0	0.0	0.0
19	1.5411952738421208	0.0	0.0	0.0	0.0
20	1.5647249726794052	0.0	0.0	0.0	0.0
21	1.5881675244839588	0.0	0.0	0.0	0.0
22	1.6091699593720534	0.0	0.0	0.0	0.0
23	1.6308695705219933	0.0	0.0	0.0	0.0
24	1.6533535049665093	0.0	0.0	0.0	0.0
25	1.6745302339200652	0.0	0.0	0.0	0.0
26	1.6983213738555416	0.0	0.0	0.0	0.0
27	1.7181908973181372	0.0	0.0	0.0	0.0
28	1.7419820372536134	0.0	0.0	0.0	0.0
29	1.7676032648764342	0.0	0.0	0.0	0.0
30	1.7913944048119106	0.0	0.0	0.0	0.0
31	1.8185842790238835	0.0	0.0	0.0	0.0
32	1.8424625659920906	0.0	0.0	0.0	0.0
33	1.864249324174761	0.0	0.0	0.0	0.0
34	1.8888247874048136	0.0	0.0	0.0	0.0
35	1.9117444570129831	0.0	0.0	0.0	0.0
36	1.9297838927882345	0.0	0.0	0.0	0.0
37	1.9446860353851811	0.0	0.0	0.0	0.0
38	1.9552308263455938	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGAC	20	3.669429E-7	206.33827	44
CGGATTA	15	3.0109832E-5	206.33827	44
GTCCCCC	10	0.0024669187	206.33827	44
GGTTCAC	10	0.0024669187	206.33827	44
GCCAGCG	15	0.008295682	137.55884	44
CGGTTCA	10	0.009518959	131.38298	43
GCAACGC	25	2.3053723E-4	123.802956	44
AACGACA	75	0.0	122.624115	43
CTTTAGA	35	5.9502727E-6	117.90757	44
GCTAATA	35	5.9502727E-6	117.90757	44
CGTTCTA	40	7.2759576E-11	114.96011	43
ACGAACA	35	3.768946E-9	112.61399	43
GCACATA	110	0.0	112.54814	44
AGGTCGA	65	9.458745E-11	111.105225	44
TACGGTG	30	1.9805884E-7	109.485825	43
CATGACC	400	0.0	108.32759	44
GCGATAT	25	1.0543328E-5	105.106384	43
TGCACCG	25	1.0543328E-5	105.106384	43
ACGGTGT	25	1.0543328E-5	105.106384	43
GCTTCGT	30	4.7619556E-4	103.169136	44
>>END_MODULE