FastQCFastQC Report
Tue 24 May 2016
ERR841025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775509
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC231171.301992836983648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA159920.9006994613938877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA152630.8596408128598616No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA147950.8332821742948079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA147190.8290017116218504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA120310.6776085055046187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA118800.6691039020359796No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT101370.5709348699443371No Hit
AAAAAAAAAAAAAAAAAAAAAAAA95680.538887721774432No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT84990.47867963496664895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77060.43401638628697464No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC57930.32627263505845366No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA48060.2706829421872826No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA45960.2588553479593739No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44210.2489990194361166No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA39930.2248932559620931No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36890.20777140527026336No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA36080.2032093332109271No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA34260.19295875154673955No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA31570.17780816655956122No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA29230.16462884727703436No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25150.14164952134852596No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23530.13252537722985352No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA23060.129878248997893No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC23000.12954031773423846No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC22360.1259357175885901No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC22200.12503456755217798No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT21730.12238743932021746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21720.12233111744294173No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA20220.11388283585157834No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC19340.10892651065131183No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA18570.10458972610107861No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA18040.10160466660546355No Hit
TAAAAAAAAAAAAAAAAAAAAAAA17850.1005345509372242No Hit
CTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGCGA17790.10019661967356967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG100.0021834187214.9459244
TGCGTAG253.5252015E-9214.9459244
GTCGATG152.559299E-5214.945944
CGACGGG152.559299E-5214.945944
CGCATAC152.559299E-5214.945944
GACCCGA304.1836756E-11214.945944
TAACCTA450.0191.0630344
TACGGCA208.058634E-5161.2094444
ACTCGAC208.058634E-5161.2094444
CTATCAT15100.0150.1774444
CGACGTC457.039489E-10143.2972744
GACAATA251.9601405E-4128.9675644
CTGCGTA251.0299664E-7122.1931843
GGGTCGT204.9897335E-6122.1931743
CGCGTAT152.4307986E-4122.1931743
TCGTGCG2550.0110.2134443
GCGACGT356.2482286E-9104.7370143
GTGACCG2000.0102.0993144
GACGAAC1750.097.7545343
CGATTGG357.474318E-492.1196844
Produced by FastQC (version 0.11.5)