##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841025.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1775509
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.79747272472288	39.0	37.0	39.0	32.0	39.0
2	37.514861372147365	39.0	37.0	39.0	35.0	39.0
3	37.90321029068284	39.0	38.0	39.0	35.0	39.0
4	38.45427198623043	39.0	38.0	40.0	35.0	41.0
5	39.128029764985705	40.0	38.0	41.0	35.0	41.0
6	39.06378508923357	40.0	38.0	41.0	35.0	41.0
7	39.08821864603333	40.0	38.0	41.0	35.0	41.0
8	39.05651506131481	40.0	38.0	41.0	35.0	41.0
9	39.04360608704321	40.0	38.0	41.0	35.0	41.0
10	39.006878590871686	40.0	38.0	41.0	35.0	41.0
11	38.93998228113741	40.0	38.0	41.0	35.0	41.0
12	38.90059695557724	40.0	38.0	41.0	35.0	41.0
13	38.76848667058292	40.0	38.0	41.0	35.0	41.0
14	38.68944511123289	40.0	38.0	41.0	35.0	41.0
15	38.59787925603306	40.0	38.0	41.0	35.0	41.0
16	38.47765570323777	40.0	37.0	41.0	35.0	41.0
17	38.39947755826639	40.0	37.0	41.0	35.0	41.0
18	38.35999704873363	40.0	37.0	41.0	35.0	41.0
19	38.338992367822414	40.0	37.0	41.0	35.0	41.0
20	38.23076650132441	40.0	36.0	41.0	35.0	41.0
21	38.14776100825172	40.0	36.0	41.0	34.0	41.0
22	38.05781834955497	40.0	36.0	41.0	34.0	41.0
23	37.94542353770102	40.0	36.0	41.0	34.0	41.0
24	37.710410930048795	39.0	35.0	41.0	33.0	41.0
25	37.625986724024955	39.0	35.0	41.0	33.0	41.0
26	37.480492660038216	39.0	35.0	41.0	33.0	41.0
27	37.40644894180968	39.0	35.0	41.0	33.0	41.0
28	37.28413540050563	39.0	35.0	41.0	33.0	41.0
29	37.29790406607875	39.0	35.0	41.0	33.0	41.0
30	37.348454221276455	39.0	35.0	41.0	33.0	41.0
31	37.35701816239181	39.0	35.0	41.0	33.0	41.0
32	37.37217053409452	39.0	35.0	40.0	33.0	41.0
33	37.25783562817706	38.0	35.0	40.0	32.0	41.0
34	37.225266936863626	38.0	35.0	40.0	32.0	41.0
35	37.0277518394662	38.0	35.0	40.0	32.0	41.0
36	36.97811293771807	38.0	35.0	40.0	32.0	41.0
37	36.94951368040484	38.0	35.0	40.0	32.0	41.0
38	36.74941516739701	38.0	35.0	40.0	31.0	41.0
39	36.64987954152775	38.0	35.0	40.0	31.0	41.0
40	36.45016249470793	37.0	34.0	40.0	31.0	41.0
41	36.367923371541934	37.0	34.0	40.0	31.0	41.0
42	36.23293481057998	37.0	34.0	40.0	31.0	41.0
43	36.07687938144112	37.0	34.0	40.0	30.0	41.0
44	35.848843822441076	37.0	34.0	40.0	30.0	41.0
45	35.597816249007096	36.0	33.0	40.0	29.0	41.0
46	35.29454676564157	36.0	33.0	40.0	28.0	41.0
47	35.33245202884196	36.0	33.0	39.0	29.0	41.0
48	36.252327904994765	37.0	34.0	40.0	31.0	41.0
49	36.947265919756234	38.0	35.0	40.0	32.0	41.0
50	36.85677560131424	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	17.0
21	74.0
22	160.0
23	347.0
24	556.0
25	876.0
26	1358.0
27	2374.0
28	4280.0
29	7771.0
30	13624.0
31	25016.0
32	46413.0
33	87896.0
34	120874.0
35	106037.0
36	189745.0
37	305129.0
38	277425.0
39	257685.0
40	327844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.17604416536329	19.008746224322152	32.81520961031456
2	20.33980114998009	27.56826352330515	32.31563455887861	19.77630076783615
3	20.277621797467656	29.45465215890204	26.960888398763398	23.30683764486691
4	18.04643062918859	31.432789132581135	30.551464396970108	19.96931584126017
5	16.642833125599477	31.98311019544255	29.213481880407254	22.160574798550726
6	17.042549488625518	33.623090617958006	25.949065873504445	23.38529401991204
7	22.435932456551896	31.18322689437226	20.620284098813354	25.760556550262486
8	21.50825481594292	31.149884343025015	26.525463965544528	20.816396875487538
9	20.975280891282445	34.73156148462216	23.723957467971157	20.569200156124243
10	24.46853268555665	29.48241884439899	23.688925260305634	22.360123209738727
11	22.433172684565385	30.24563660336275	23.20596516266603	24.11522554940583
12	22.522724469433836	31.330790212834742	23.62224015761114	22.52424516012028
13	21.66099974711477	30.416686144649223	23.42280439017769	24.499509718058317
14	19.19072220980012	30.972808360870037	22.658122262404753	27.178347166925086
15	19.464164923973915	36.9463066647367	23.264765202541916	20.324763208747463
16	19.79229618098247	38.30873287603724	20.00310896762562	21.895861975354673
17	19.28894756376904	38.42081341181599	20.59595304783023	21.694285976584744
18	19.915472126584547	32.75731072047508	21.5719548591418	25.75526229379857
19	21.65052387794148	31.39972819062027	19.918062932939232	27.031684998499024
20	20.809807215846273	29.979515733234805	24.19052789932352	25.0201491515954
21	22.20602655351226	36.308574048343324	21.26607074365717	20.21932865448725
22	22.965020171680347	32.67034974196132	19.961374456564286	24.40325562979405
23	23.66695972816809	32.169817218611676	23.397459545403603	20.765763507816633
24	22.916076460327712	33.17054433404731	22.296592132171675	21.616787073453303
25	22.471040711090854	31.835446283581664	21.43631235577334	24.257200649554143
26	22.553663969740793	37.691879975816676	21.21468015476401	18.539775899678517
27	25.28206548682871	31.84162813168408	21.03560156033599	21.84070482115122
28	23.138459027920266	29.459185105992375	21.48410924288075	25.918246623206613
29	22.21527134634725	31.050378683309592	24.90740270321059	21.826947267132564
30	23.85246989878062	31.080003764795052	25.32317087993358	19.74435545649075
31	23.305448970730282	31.166092142458375	22.93953000839316	22.588928878418187
32	22.150675945760348	29.29288335195302	24.373955845726528	24.18248485656011
33	22.247579055284657	26.424768520016723	29.244339047466177	22.083313377232443
34	23.572598481054914	30.185903855783675	24.591183650475713	21.650314012685694
35	21.373490001465395	26.672769197508433	24.34140965833932	27.612331142686852
36	21.721806950522712	27.955892186786198	25.91271128648892	24.409589576202173
37	20.6223237758024	34.1787491134799	23.033591779492458	22.16533533122524
38	25.370729514082285	28.20953314051417	22.507968889455565	23.91176845594798
39	22.301108326100415	33.19286604639341	23.854495358766158	20.65153026874001
40	21.282136762799993	29.079292099832493	24.774801491937986	24.863769645429525
41	22.237614350270032	32.517083654799954	23.25367849663838	21.991623498291634
42	20.388368421418683	33.26920803165926	22.678239823064246	23.66418372385781
43	21.59388432396394	26.258517976109847	24.893738930771555	27.253858769154654
44	22.504067498884137	27.453169769484397	23.244352295797157	26.798410435834306
45	21.017161836857472	28.659986844690728	29.080820181185175	21.242031137266622
46	20.733828207847296	29.18252386002121	26.540487804878047	23.543160127253447
47	21.27878219255967	31.445292487950706	25.63933082469608	21.636594494793542
48	24.232307315024123	27.192844013975932	22.757866315730688	25.816982355269257
49	20.12418164440069	28.516144332415866	23.770041178288334	27.589632844895114
50	19.39536254420506	31.34232901106067	27.289257856587497	21.973050588146773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	145194.0
1	140911.5
2	91812.0
3	45508.5
4	41959.0
5	30258.5
6	14305.0
7	8235.0
8	7949.5
9	6650.5
10	5355.0
11	4825.5
12	4817.5
13	4805.0
14	4976.0
15	5402.5
16	6030.5
17	6603.0
18	6927.0
19	7263.5
20	7548.5
21	8851.0
22	10972.5
23	13161.0
24	15025.5
25	16719.0
26	18767.5
27	21063.0
28	22758.5
29	24425.5
30	27989.5
31	34422.5
32	42592.0
33	47689.0
34	53481.5
35	61056.0
36	64072.0
37	64373.5
38	64124.0
39	67831.0
40	74706.5
41	79774.0
42	85345.5
43	88674.0
44	88113.0
45	87752.5
46	92348.0
47	97271.0
48	108149.5
49	125319.5
50	145119.0
51	160549.0
52	152011.5
53	133041.0
54	121233.0
55	113586.5
56	111979.0
57	113499.0
58	110804.5
59	99950.0
60	85251.0
61	76474.0
62	68994.5
63	57826.5
64	49023.5
65	44177.0
66	34375.5
67	23873.5
68	17584.5
69	13144.0
70	10558.5
71	7787.0
72	5238.5
73	3262.5
74	1911.5
75	1452.0
76	1167.0
77	842.0
78	699.5
79	715.5
80	629.5
81	393.5
82	213.0
83	148.5
84	93.5
85	75.5
86	64.0
87	42.0
88	17.5
89	11.5
90	7.5
91	3.0
92	1.5
93	1.0
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	20459.0
25	24932.0
26	29715.0
27	33585.0
28	33131.0
29	29350.0
30	24479.0
31	18786.0
32	16014.0
33	11362.0
34	11923.0
35	11383.0
36	10142.0
37	10078.0
38	10822.0
39	12531.0
40	15137.0
41	13870.0
42	18899.0
43	29851.0
44	70961.0
45	139349.0
46	183256.0
47	171792.0
48	267098.0
49	237636.0
50	318968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.939850775238305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.89851782004503	8.226380214064347
2	11.525259114343111	2.752197479424239
3	4.296869341271103	1.5391193620642045
4	2.252099023026938	1.0755890506400645
5	1.3749884712407372	0.8208578582143725
6	0.978692227792099	0.7011263492833918
7	0.7299562821488421	0.6100898356913447
8	0.6107267378026616	0.5833588891049497
9	0.49878524591648665	0.5359879264620054
>10	5.0638138607685175	14.205859741146126
>50	2.136867183788204	18.319058450532427
>100	1.5399712951520825	30.01272799406071
>500	0.05325838736904429	4.350196725697367
>1k	0.0341657579348586	7.29890002374884
>5k	0.002009750466756388	1.8936538625575823
>10k+	0.004019500933512776	7.0748962373080495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23117	1.301992836983648	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	15992	0.9006994613938877	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	15263	0.8596408128598616	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	14795	0.8332821742948079	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	14719	0.8290017116218504	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	12031	0.6776085055046187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11880	0.6691039020359796	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	10137	0.5709348699443371	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	9568	0.538887721774432	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8499	0.47867963496664895	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7706	0.43401638628697464	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5793	0.32627263505845366	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	4806	0.2706829421872826	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4596	0.2588553479593739	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4421	0.2489990194361166	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	3993	0.2248932559620931	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3689	0.20777140527026336	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3608	0.2032093332109271	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3426	0.19295875154673955	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	3157	0.17780816655956122	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2923	0.16462884727703436	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2515	0.14164952134852596	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2353	0.13252537722985352	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	2306	0.129878248997893	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2300	0.12954031773423846	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2236	0.1259357175885901	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2220	0.12503456755217798	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	2173	0.12238743932021746	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2172	0.12233111744294173	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	2022	0.11388283585157834	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1934	0.10892651065131183	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA	1857	0.10458972610107861	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1804	0.10160466660546355	No Hit
TAAAAAAAAAAAAAAAAAAAAAAA	1785	0.1005345509372242	No Hit
CTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGCGA	1779	0.10019661967356967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.14294492452586835	0.0	0.0	0.0	0.0
2	0.2758645548966522	0.0	0.0	0.0	0.0
3	0.3811301435250399	0.0	0.0	0.0	0.0
4	0.4524336401561468	0.0	0.0	0.0	0.0
5	0.509375058081936	0.0	0.0	0.0	0.0
6	0.5834946485768306	0.0	0.0	0.0	0.0
7	0.6475889449166408	0.0	0.0	0.0	0.0
8	0.7129223225565176	0.0	0.0	0.0	0.0
9	0.7764534001235702	0.0	0.0	0.0	0.0
10	0.8437017215908227	0.0	0.0	0.0	0.0
11	0.912358089989969	0.0	0.0	0.0	0.0
12	0.9851259554302456	0.0	0.0	0.0	0.0
13	1.0538949675839435	0.0	0.0	0.0	0.0
14	1.112357076196178	0.0	0.0	0.0	0.0
15	1.1699743566492764	0.0	0.0	0.0	0.0
16	1.2190870336337354	0.0	0.0	0.0	0.0
17	1.262116947872413	0.0	0.0	0.0	0.0
18	1.302218124492751	0.0	0.0	0.0	0.0
19	1.3331951569944167	0.0	0.0	0.0	0.0
20	1.3603423018413312	0.0	0.0	0.0	0.0
21	1.386475652897282	0.0	0.0	0.0	0.0
22	1.4102434851076509	0.0	0.0	0.0	0.0
23	1.4368274111818076	0.0	0.0	0.0	0.0
24	1.4631860497468614	0.0	0.0	0.0	0.0
25	1.4852642256389577	0.0	0.0	0.0	0.0
26	1.5122424048540446	0.0	0.0	0.0	0.0
27	1.537080352732653	0.0	0.0	0.0	0.0
28	1.5646217507204976	0.0	0.0	0.0	0.0
29	1.5944723456766483	0.0	0.0	0.0	0.0
30	1.6231965030872837	0.0	0.0	0.0	0.0
31	1.6513574417251615	0.0	0.0	0.0	0.0
32	1.6776597584129396	0.0	0.0	0.0	0.0
33	1.704581615750751	0.0	0.0	0.0	0.0
34	1.7285184135929472	0.0	0.0	0.0	0.0
35	1.7509908426259737	0.0	0.0	0.0	0.0
36	1.7720552247271064	0.0	0.0	0.0	0.0
37	1.789683972314418	0.0	0.0	0.0	0.0
38	1.8036517978788054	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	10	0.0021834187	214.94592	44
TGCGTAG	25	3.5252015E-9	214.94592	44
GTCGATG	15	2.559299E-5	214.9459	44
CGACGGG	15	2.559299E-5	214.9459	44
CGCATAC	15	2.559299E-5	214.9459	44
GACCCGA	30	4.1836756E-11	214.9459	44
TAACCTA	45	0.0	191.06303	44
TACGGCA	20	8.058634E-5	161.20944	44
ACTCGAC	20	8.058634E-5	161.20944	44
CTATCAT	1510	0.0	150.17744	44
CGACGTC	45	7.039489E-10	143.29727	44
GACAATA	25	1.9601405E-4	128.96756	44
CTGCGTA	25	1.0299664E-7	122.19318	43
GGGTCGT	20	4.9897335E-6	122.19317	43
CGCGTAT	15	2.4307986E-4	122.19317	43
TCGTGCG	255	0.0	110.21344	43
GCGACGT	35	6.2482286E-9	104.73701	43
GTGACCG	200	0.0	102.09931	44
GACGAAC	175	0.0	97.75453	43
CGATTGG	35	7.474318E-4	92.11968	44
>>END_MODULE