FastQCFastQC Report
Tue 24 May 2016
ERR841024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537911
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC123490.4865812867354292No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA83950.33078386121499137No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG51960.20473531183717633No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT45720.18014816122393576No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC44400.1749470332095964No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA39770.15670368267445153No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT38990.15363028884779648No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA38590.15205418944951182No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT37990.14969004035208486No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36140.14240058063501834No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA35620.14035165141724829No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT35280.13901196692870632No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG34300.13515052340290892No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT33650.13258936188069637No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT30840.12151726360774667No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC30810.12139905615287534No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC29250.1152522684995652No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC28690.11304572934196669No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG28460.112139472187953No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC27840.10969651812061179No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC27510.10839623611702696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGT100.0023475117209.8156344
CACGCTA150.007894606139.8770844
CTAGGCG355.4831307E-6119.8946444
CGTACGT206.5779095E-6115.5946943
GTACGTA401.0647946E-5104.90781444
AGGCATA855.456968E-1298.7367644
CCCTCCG1000.094.4170344
CTATCAT8050.089.92097544
ACGTCAA402.3241228E-886.69601443
TAACCGA251.5137102E-677.8251242
TCGTGCG3700.076.5424243
ACGTCCA555.1714553E-576.2965944
CGCTTAA701.8938626E-674.9341544
TAATCGG856.927439E-874.0525744
CCGTTTA900.073.50149542
GCCCTAG2650.071.2581444
AACGACA3050.070.114843
CGTGCGC2000.068.1900844
TATCACG351.05657135E-466.054143
GTAACCG254.7398953E-664.21886441
Produced by FastQC (version 0.11.5)