##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841024.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2537911
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.30579874550369	39.0	37.0	39.0	34.0	39.0
2	37.857529282941755	39.0	38.0	39.0	35.0	39.0
3	38.074239403982254	39.0	38.0	39.0	35.0	39.0
4	38.60584315210423	39.0	38.0	40.0	35.0	41.0
5	39.27061784278487	40.0	39.0	41.0	36.0	41.0
6	39.23252430837803	40.0	39.0	41.0	35.0	41.0
7	39.236492138613215	40.0	39.0	41.0	35.0	41.0
8	39.18934548926263	40.0	38.0	41.0	35.0	41.0
9	39.214680499040355	40.0	39.0	41.0	35.0	41.0
10	39.18227392528737	40.0	39.0	41.0	35.0	41.0
11	39.1267518837343	40.0	38.0	41.0	35.0	41.0
12	39.07017818985772	40.0	38.0	41.0	35.0	41.0
13	38.935229801202645	40.0	38.0	41.0	35.0	41.0
14	38.93329513919125	40.0	38.0	41.0	35.0	41.0
15	38.93446027067143	40.0	38.0	41.0	35.0	41.0
16	38.83705338760894	40.0	38.0	41.0	35.0	41.0
17	38.83621096248056	40.0	38.0	41.0	35.0	41.0
18	38.80592739461707	40.0	38.0	41.0	35.0	41.0
19	38.78286748432077	40.0	38.0	41.0	35.0	41.0
20	38.70918050317761	40.0	38.0	41.0	35.0	41.0
21	38.614974283968195	40.0	38.0	41.0	35.0	41.0
22	38.52649560997214	40.0	38.0	41.0	35.0	41.0
23	38.41812537949518	40.0	38.0	41.0	34.0	41.0
24	38.29573101657229	40.0	37.0	41.0	34.0	41.0
25	38.165505385811954	40.0	37.0	41.0	34.0	41.0
26	38.03704682399227	39.0	37.0	41.0	34.0	41.0
27	37.9060633793296	39.0	36.0	41.0	33.0	41.0
28	37.80264057707965	39.0	36.0	41.0	33.0	41.0
29	37.700001052196214	39.0	36.0	41.0	33.0	41.0
30	37.6633692861355	39.0	36.0	41.0	33.0	41.0
31	37.56537279972751	39.0	36.0	41.0	33.0	41.0
32	37.47591138621791	39.0	35.0	40.0	33.0	41.0
33	37.382543469013015	38.0	35.0	40.0	33.0	41.0
34	37.29163292235011	38.0	35.0	40.0	32.0	41.0
35	37.17936128127531	38.0	35.0	40.0	32.0	41.0
36	37.09341911447061	38.0	35.0	40.0	32.0	41.0
37	37.035860624485274	38.0	35.0	40.0	32.0	41.0
38	36.887537506019484	38.0	35.0	40.0	32.0	41.0
39	36.72159670625365	38.0	35.0	40.0	31.0	41.0
40	36.5382739739738	38.0	34.0	40.0	31.0	41.0
41	36.43085047821749	37.0	34.0	40.0	31.0	41.0
42	36.27325487808676	37.0	34.0	40.0	31.0	41.0
43	36.124594378364485	37.0	34.0	40.0	30.0	41.0
44	35.8936328137169	37.0	34.0	40.0	30.0	41.0
45	35.63176311107749	36.0	34.0	40.0	29.0	41.0
46	35.285714894251456	36.0	33.0	40.0	28.0	41.0
47	35.35593742440735	36.0	33.0	39.0	29.0	41.0
48	36.20662188261236	37.0	34.0	40.0	31.0	41.0
49	36.75018671417315	38.0	35.0	40.0	32.0	41.0
50	36.79762000482432	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	17.0
22	49.0
23	105.0
24	209.0
25	401.0
26	696.0
27	1455.0
28	3179.0
29	6562.0
30	14070.0
31	27491.0
32	50809.0
33	88172.0
34	136797.0
35	153660.0
36	261437.0
37	429750.0
38	446628.0
39	419831.0
40	496587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.26124044538993	19.90038263753142	34.83837691707865
2	23.17496555237753	20.17336305331432	33.75768496215982	22.89398643214833
3	21.04072995467532	19.920359697404677	29.66416079996501	29.37474954795499
4	18.887659969163614	21.697648183880364	37.24709022499213	22.16760162196389
5	17.915246042906944	23.045410181838527	34.80082634891452	24.23851742634001
6	21.198261089533872	25.942123265945888	29.784338379084215	23.07527726543602
7	26.818355726422244	25.002373999718667	24.44183424871873	23.73743602514036
8	26.145715905719307	22.99335949921018	27.517592224471226	23.343332370599285
9	25.253683048775155	24.241078587862223	27.077151247620584	23.42808711574204
10	23.675889343637348	21.968461462990625	28.722165592095234	25.633483601276797
11	25.3956896045606	24.152620009133496	27.590605029096764	22.861085357209138
12	25.15458579910801	24.555943845154538	26.969858281082352	23.3196120746551
13	24.7641859781529	22.678257827008117	28.788558779247975	23.768997415591013
14	21.189907762722964	24.278707960996268	26.884315486240457	27.647068790040315
15	23.229380384103305	27.777372807793498	24.871872969540696	24.1213738385625
16	23.477655441818094	31.533454088815567	22.54909648131869	22.43979398804765
17	22.834961509682568	29.733390966034662	22.90395526084248	24.527692263440287
18	23.739563759328046	24.882275225569376	24.485689214476	26.892471800626577
19	23.560912892532482	24.42461536279247	24.383400363527326	27.631071381147727
20	23.592868307832703	24.01289879747556	27.01351623441484	25.380716660276896
21	24.89547505803001	26.38090933842834	25.15383715189382	23.56977845164783
22	25.296592354893455	26.96225360148563	23.035165535749677	24.705988507871236
23	26.785257639058262	25.372717955830602	24.385055267895524	23.45696913721561
24	26.895269377058533	25.898819934978018	23.775380618154067	23.430530069809382
25	25.635926475659428	27.144476429872004	24.41674984153554	22.802847252933027
26	28.272086081105762	28.772883401060096	22.143686328320715	20.811344189513424
27	27.19236913703602	26.199752165749757	24.383114311747967	22.22476438546626
28	25.71901640293882	27.037575184158953	24.039300115531784	23.204108297370446
29	25.8689724332685	27.319465387196068	24.389867836061356	22.421694343474076
30	27.05852955575913	27.81691215581293	24.625437947129317	20.499120341298624
31	24.576362085143526	29.426786951231694	24.995537636961792	21.001313326662988
32	23.1948827060319	28.595400150959183	25.587046334916085	22.622670808092828
33	23.97188126374425	27.10220525219421	26.922931946975197	22.002981537086335
34	23.77243207659436	27.48743670468376	26.532130765697925	22.208000453023953
35	23.703601596650167	26.383609155054977	25.767121525658144	24.14566772263671
36	23.82913172939746	27.099154762180717	25.922860195430953	23.14885331299087
37	23.986134976394553	29.13171602967542	23.78596314635378	23.096185847576248
38	25.648773770843597	27.685371385701323	22.574972654189285	24.090882189265795
39	23.041789492531073	30.242276426943132	23.16947699097835	23.54645708954744
40	21.964329163596457	29.902000541852964	24.122120543302508	24.011549751248072
41	23.60204228609673	30.90996439097603	22.86557995973043	22.622413363196813
42	22.15469610836421	31.80801608462675	22.968445236082864	23.068842570926176
43	24.375771517726346	27.044973527384585	25.831554825684087	22.74770012920498
44	24.746817354981236	28.2233127139202	24.0638980578382	22.96597187326036
45	24.800718664190548	26.68935331166492	26.987496175673048	21.52243184847148
46	22.66485271879193	28.612968839854314	24.56561095610317	24.15656748525058
47	23.01411818658281	30.07390441057894	24.159850729721015	22.75212667311724
48	24.65528868282583	28.8181260732805	23.895830434297267	22.63075480959641
49	20.53159295168271	29.028058515194076	24.77200315187228	25.66834538125093
50	21.589632259407928	30.90674435050088	25.69442296868525	21.809200421405937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28333.0
1	25175.0
2	27077.0
3	30809.0
4	26566.5
5	17131.0
6	7850.0
7	5411.0
8	6113.5
9	6349.5
10	5860.5
11	5049.0
12	4382.5
13	4248.5
14	4628.5
15	5010.0
16	5129.5
17	5477.5
18	6537.5
19	7851.5
20	8837.0
21	10062.0
22	11551.5
23	14294.0
24	17467.0
25	20472.5
26	24240.5
27	28171.5
28	31001.0
29	35544.0
30	43395.5
31	53142.5
32	63487.5
33	71175.5
34	86614.5
35	103802.5
36	107975.5
37	107131.0
38	106943.0
39	110629.0
40	117212.5
41	122183.5
42	126219.0
43	128592.0
44	131842.0
45	133238.0
46	140159.5
47	155907.5
48	173679.0
49	192181.5
50	218521.5
51	234639.0
52	226641.0
53	217143.5
54	210309.5
55	200990.0
56	199147.5
57	199662.5
58	190722.0
59	171414.5
60	145567.5
61	127703.5
62	115191.5
63	95571.0
64	73757.0
65	59464.5
66	43969.0
67	31009.5
68	24774.5
69	19208.5
70	14350.0
71	10216.5
72	7030.0
73	4937.0
74	3577.0
75	2613.0
76	1959.0
77	1304.5
78	787.5
79	503.5
80	253.5
81	204.0
82	238.0
83	215.5
84	208.0
85	120.5
86	45.0
87	39.5
88	23.5
89	20.0
90	10.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	12108.0
25	13010.0
26	12735.0
27	14162.0
28	14874.0
29	13819.0
30	14551.0
31	14181.0
32	14280.0
33	12552.0
34	14892.0
35	14042.0
36	12233.0
37	11849.0
38	14017.0
39	16634.0
40	19959.0
41	18072.0
42	23836.0
43	41202.0
44	113226.0
45	223637.0
46	290584.0
47	279550.0
48	432236.0
49	390627.0
50	485043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.795892887710089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49066641594001	8.432962435134412
2	10.830131612722953	2.5550214492696575
3	3.907073643605641	1.3826226661310195
4	1.8716270973497702	0.8831005106429454
5	1.1023415484872856	0.6501551415814248
6	0.7047962068688556	0.4988220338333628
7	0.5626583906831919	0.4645940676209188
8	0.4366300943790053	0.4120353459876395
9	0.347057618056894	0.3684469039616628
>10	4.321670049305088	12.674256688653212
>50	2.4589089671930897	21.00774587188446
>100	1.861592255119894	35.60075818361813
>500	0.06581580018101128	5.2008638742545905
>1k	0.03788235010418673	8.697761455749122
>5k	7.653000021047825E-4	0.613456753063547
>10k+	3.8265000105239125E-4	0.5573966186139168
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12349	0.4865812867354292	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	8395	0.33078386121499137	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	5196	0.20473531183717633	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4572	0.18014816122393576	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	4440	0.1749470332095964	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	3977	0.15670368267445153	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT	3899	0.15363028884779648	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3859	0.15205418944951182	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3799	0.14969004035208486	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3614	0.14240058063501834	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	3562	0.14035165141724829	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	3528	0.13901196692870632	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	3430	0.13515052340290892	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	3365	0.13258936188069637	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3084	0.12151726360774667	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	3081	0.12139905615287534	No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC	2925	0.1152522684995652	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	2869	0.11304572934196669	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	2846	0.112139472187953	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	2784	0.10969651812061179	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2751	0.10839623611702696	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03711714082960356	0.0	0.0	0.0	0.0
2	0.07053044807323819	0.0	0.0	0.0	0.0
3	0.08912842097299709	0.0	0.0	0.0	0.0
4	0.10670192926387095	0.0	0.0	0.0	0.0
5	0.12112323875817552	0.0	0.0	0.0	0.0
6	0.14937482047242792	0.0	0.0	0.0	0.0
7	0.17120379713867034	0.0	0.0	0.0	0.0
8	0.19082623464731427	0.0	0.0	0.0	0.0
9	0.2129310287082565	0.0	0.0	0.0	0.0
10	0.23251406373194333	0.0	0.0	0.0	0.0
11	0.2510726341467451	0.0	0.0	0.0	0.0
12	0.27030104680581785	0.0	0.0	0.0	0.0
13	0.2904751191038614	0.0	0.0	0.0	0.0
14	0.3119494734054898	0.0	0.0	0.0	0.0
15	0.3338572550416465	0.0	0.0	0.0	0.0
16	0.35493758449370366	0.0	0.0	0.0	0.0
17	0.3799581624414725	0.0	0.0	0.0	3.940248495711631E-5
18	0.4026539937767715	0.0	0.0	0.0	3.940248495711631E-5
19	0.42515281268728494	0.0	0.0	0.0	3.940248495711631E-5
20	0.44666656947387046	0.0	0.0	0.0	3.940248495711631E-5
21	0.46723466662148516	0.0	0.0	0.0	3.940248495711631E-5
22	0.48776336128414277	0.0	0.0	0.0	3.940248495711631E-5
23	0.5130991591115686	0.0	0.0	0.0	3.940248495711631E-5
24	0.5363466252362672	0.0	0.0	0.0	3.940248495711631E-5
25	0.5618006305185643	0.0	0.0	0.0	3.940248495711631E-5
26	0.5899734072629025	0.0	0.0	0.0	3.940248495711631E-5
27	0.6146393628460572	0.0	0.0	0.0	3.940248495711631E-5
28	0.6430879569850952	0.0	0.0	0.0	3.940248495711631E-5
29	0.6722457958533613	0.0	0.0	0.0	3.940248495711631E-5
30	0.6999063402932569	0.0	0.0	0.0	3.940248495711631E-5
31	0.7254785530304254	0.0	0.0	0.0	3.940248495711631E-5
32	0.7552274291730482	0.0	0.0	0.0	3.940248495711631E-5
33	0.7848580978607997	0.0	0.0	0.0	3.940248495711631E-5
34	0.8091694310793405	0.0	0.0	0.0	3.940248495711631E-5
35	0.8321804822942964	0.0	0.0	0.0	3.940248495711631E-5
36	0.8520787371976402	0.0	0.0	0.0	3.940248495711631E-5
37	0.8712283448867987	0.0	0.0	0.0	3.940248495711631E-5
38	0.8844281773474326	0.0	0.0	0.0	3.940248495711631E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCGT	10	0.0023475117	209.81563	44
CACGCTA	15	0.007894606	139.87708	44
CTAGGCG	35	5.4831307E-6	119.89464	44
CGTACGT	20	6.5779095E-6	115.59469	43
GTACGTA	40	1.0647946E-5	104.907814	44
AGGCATA	85	5.456968E-12	98.73676	44
CCCTCCG	100	0.0	94.41703	44
CTATCAT	805	0.0	89.920975	44
ACGTCAA	40	2.3241228E-8	86.696014	43
TAACCGA	25	1.5137102E-6	77.82512	42
TCGTGCG	370	0.0	76.54242	43
ACGTCCA	55	5.1714553E-5	76.29659	44
CGCTTAA	70	1.8938626E-6	74.93415	44
TAATCGG	85	6.927439E-8	74.05257	44
CCGTTTA	90	0.0	73.501495	42
GCCCTAG	265	0.0	71.25814	44
AACGACA	305	0.0	70.1148	43
CGTGCGC	200	0.0	68.19008	44
TATCACG	35	1.05657135E-4	66.0541	43
GTAACCG	25	4.7398953E-6	64.218864	41
>>END_MODULE