FastQCFastQC Report
Tue 24 May 2016
ERR841023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362025
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT32430.8957944893308473No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT15990.441682204267661No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC10330.28533941026172227No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG9510.2626890408120986No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA9140.25246875215800013No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC8780.24252468752158No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG8280.22871348663766317No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT8210.2267799185139148No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC7490.20689178924107451No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA6760.18672743595055588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.16241972239486224No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA5760.15910503418272218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA5680.15689524204129549No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT5580.15413300186451212No Hit
TCTGCTGCTATTCTCCGAGCTTCGCAATGCCGCCTAAGGACGACAAGAA5550.15330432981147712No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA5520.1524756577584421No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT5450.15054208963469373No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA5440.1502658656170154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA5360.14805607347558872No Hit
ATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCA5330.1472274014225537No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC5210.14391271321041366No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT5170.14280781713970028No Hit
CTCTTTCCCATCTTGCAAGATGGCGGG5150.14225536910434364No Hit
CTCATTGAACTCGCCTGCAGCTCTTGGG5070.14004557696291692No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC4960.13700711276845523No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC4770.13175885643256682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4530.12512948000828672No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC4530.12512948000828672No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC4470.1234721359022167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.12319591188453835No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC4210.11629031144257994No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC4080.11269939921276155No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGTCC4070.11242317519508321No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTT4040.1115945031420482No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG3960.10938471100062151No Hit
AAAAAAAAAAAAAAAAAAAAAAAA3940.10883226296526483No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC3920.10827981492990817No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT3890.10745114287687314No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT3880.10717491885919482No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC3830.10579379877080311No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGT3760.10386023064705475No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3630.10026931841723638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG100.0024082374207.9028544
TACTTTA152.9130595E-5207.9028544
GACCGAC152.9130595E-5207.9028544
CCGTGAC152.9130595E-5207.9028544
CCACTAC152.9130595E-5207.9028544
AGGCCGT305.2750693E-11207.9028544
GTAGAGA305.2750693E-11207.9028544
CGGGCAC100.0024082374207.9028544
CATAATC100.0024082374207.9028544
ATGCGTA100.0024082374207.9028544
AAAGGTC100.0024082374207.9028544
GGATTCG100.0024082374207.9028544
TCAAGTG100.0024082374207.9028544
GCATCCC100.0024082374207.9028544
TGGTAGA100.0024082374207.9028544
AAAAACG254.267349E-9207.9028344
GTAGCTA254.267349E-9207.9028344
CCAACGG254.267349E-9207.9028344
CCCGAGC1350.0184.8025444
AATCGCA401.8189894E-12181.91544
Produced by FastQC (version 0.11.5)