FastQCFastQC Report
Tue 24 May 2016
ERR841022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401428
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG143981.0273806431725354No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTT120510.8599086075060581No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT97410.695076736014979No Hit
AAGCAGTGGTATCAACGCAGAGTG87080.6213662064694012No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC81200.5794090028171266No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG77100.5501531295221731No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT76570.5463712727303864No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC61760.4406933499259327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA57530.41050985138016366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA57160.4078696872047654No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA55190.39381259686548287No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA53640.38275244964422006No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA49930.3562794521017134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA49550.3535679321377909No Hit
AAGCAGTGGTATCAACGCAGAGTGCA49250.3514272584820626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40160.28656484671349514No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTA39290.280356893111883No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT32970.2352600347645402No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31350.22370039702360733No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT30740.2193476939236265No Hit
AAAAAAAAAAAAAAAAAAAAAAAA30660.21877684761543226No Hit
AAGCAGTGGTATCAACGCAGAGTGC28660.20450568991057694No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGCATGGGAAGCAGTGGT27830.19858315946306193No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTT25990.18545369437459505No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21910.15634053265669018No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC19870.14178395179773773No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC19120.13643226765841698Illumina Single End Adapter 2 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18980.1354332866190771No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT17240.12301737941585297No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG14570.1039653838798711No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT14270.10182471022414281Illumina Single End Adapter 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC100.0034497147184.4763844
GGAATCG600.0138.3572844
TGCTCGA201.4815309E-4138.3572844
CGATATT201.4815309E-4138.3572844
CTACCCT700.0131.7688444
CCGCTCA152.3698839E-4122.9701243
CACCGTG152.3698839E-4122.9701243
TATCGTT152.3698839E-4122.9701243
CTCGACA204.833708E-6122.97011643
TCGTGCG1300.0113.5108943
TCGATTT253.6013906E-4110.6858344
CCACTAC601.2187229E-10107.6112244
TACTGGC601.2187229E-10107.6112244
CTATCAT6050.0106.7218844
GATCCAA351.0388701E-5105.4150844
CACGAAA351.0388701E-5105.4150844
ACTGGGC351.0388701E-5105.4150844
ATGCGGA452.9352123E-7102.48688544
CTTATGT402.0163352E-592.2381944
ATGCACG307.435583E-492.2381944
Produced by FastQC (version 0.11.5)