##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841022.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1401428
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.183133204131785	39.0	37.0	39.0	34.0	39.0
2	37.775269938947986	39.0	37.0	39.0	35.0	39.0
3	37.97283413775092	39.0	38.0	39.0	35.0	39.0
4	38.30373019520089	39.0	38.0	40.0	35.0	41.0
5	39.21449478674609	40.0	39.0	41.0	35.0	41.0
6	39.18136643480793	40.0	38.0	41.0	35.0	41.0
7	38.77789155061837	40.0	38.0	41.0	35.0	41.0
8	38.9955031582072	40.0	38.0	41.0	35.0	41.0
9	39.13733991328844	40.0	38.0	41.0	35.0	41.0
10	38.56807556292581	40.0	38.0	41.0	34.0	41.0
11	38.83158892215654	40.0	38.0	41.0	35.0	41.0
12	38.87529648330132	40.0	38.0	41.0	35.0	41.0
13	38.80714956458698	40.0	38.0	41.0	35.0	41.0
14	38.887613919516376	40.0	38.0	41.0	35.0	41.0
15	38.87604928687025	40.0	38.0	41.0	35.0	41.0
16	38.618556215517316	40.0	38.0	41.0	35.0	41.0
17	38.49246911007915	40.0	38.0	41.0	35.0	41.0
18	38.5643193942179	40.0	38.0	41.0	35.0	41.0
19	38.46305910828098	40.0	38.0	41.0	35.0	41.0
20	38.45843882097404	40.0	37.0	41.0	35.0	41.0
21	38.21237908761635	40.0	37.0	41.0	34.0	41.0
22	38.17541750271866	40.0	37.0	41.0	34.0	41.0
23	37.626086391880285	39.0	36.0	41.0	33.0	41.0
24	37.65092320119193	39.0	36.0	41.0	33.0	41.0
25	37.66225612129578	39.0	36.0	41.0	33.0	41.0
26	37.65428263817475	39.0	36.0	41.0	33.0	41.0
27	37.58381339265468	39.0	36.0	41.0	33.0	41.0
28	37.222860237942825	39.0	35.0	41.0	32.0	41.0
29	37.2626674924869	39.0	35.0	41.0	32.0	41.0
30	37.28054556759904	39.0	35.0	41.0	32.0	41.0
31	37.32543414744594	39.0	35.0	41.0	32.0	41.0
32	37.280075385801524	39.0	35.0	40.0	32.0	41.0
33	37.218620295309066	38.0	35.0	40.0	32.0	41.0
34	37.180065050480216	38.0	35.0	40.0	32.0	41.0
35	37.15185925840345	38.0	35.0	40.0	32.0	41.0
36	37.17534190948437	38.0	35.0	40.0	32.0	41.0
37	37.095011398569994	38.0	35.0	40.0	32.0	41.0
38	36.95844504487587	38.0	35.0	40.0	32.0	41.0
39	36.793621519420974	38.0	35.0	40.0	31.0	41.0
40	36.61644804549489	38.0	35.0	40.0	31.0	41.0
41	36.51415599986053	38.0	34.0	40.0	31.0	41.0
42	36.39680482462276	37.0	34.0	40.0	31.0	41.0
43	36.246873049226	37.0	34.0	40.0	31.0	41.0
44	36.033622424265005	37.0	34.0	40.0	30.0	41.0
45	35.805546847466076	37.0	34.0	40.0	29.0	41.0
46	35.400472089589236	36.0	33.0	40.0	28.0	41.0
47	35.304904320888056	36.0	33.0	40.0	28.0	41.0
48	36.1400193408622	37.0	34.0	40.0	31.0	41.0
49	36.949817238750455	38.0	35.0	40.0	32.0	41.0
50	36.40110778713273	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	14.0
22	21.0
23	63.0
24	141.0
25	276.0
26	643.0
27	1403.0
28	2848.0
29	6123.0
30	12541.0
31	22065.0
32	36707.0
33	56794.0
34	82189.0
35	86386.0
36	143440.0
37	238680.0
38	242636.0
39	209925.0
40	258527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	57.80503886036244	14.386111880167944	27.808849259469625
2	19.630334201971134	35.18932117811261	28.058237740361974	17.122106879554284
3	30.9311644979264	21.71206797637838	24.81561664245327	22.54115088324195
4	16.60599046115819	23.55961205284895	28.495720079804315	31.33867740618855
5	15.759139962952073	37.44009681553387	26.529868105960492	20.270895115553564
6	28.150143995981242	27.910602613905244	23.482690512819783	20.45656287729373
7	22.51246585625519	26.222467368997908	31.30792306133458	19.95714371341232
8	33.57011562491973	26.02409827690042	22.25729755649238	18.14848854168748
9	32.42064522758215	26.715607223489187	22.035951900490073	18.82779564843859
10	21.172190080403702	23.31514712136478	34.20239926703334	21.310263531198178
11	20.952628319114503	35.90223686125866	22.758643326663947	20.38649149296289
12	21.663117905450726	24.657563570871996	33.53258248015595	20.146736043521322
13	19.572179234323848	23.742711006202242	22.447817511852197	34.23729224762171
14	17.841944074187186	37.1430426679073	22.27684904254803	22.73816421535748
15	18.619650813313278	41.00631641440017	21.833301461081124	18.540731311205427
16	19.099661202716085	32.2256298575453	18.014553726627412	30.660155213111196
17	29.857045813270467	30.16715807019697	19.570966185918934	20.40482993061363
18	18.131862642961323	26.438175917706797	21.038183909555112	34.39177752977677
19	20.295227439440342	35.76851611356416	20.054972499479103	23.88128394751639
20	30.059624896890885	24.794994819569755	22.681507719269202	22.46387256427016
21	20.87228170123617	37.57281858218903	21.21785778505924	20.337041931515568
22	31.232999483384088	27.135607394743076	20.458917618315034	21.1724755035578
23	22.564912360820536	26.395719223534854	30.980828126739297	20.058540288905316
24	31.56216373584658	26.821142434716588	22.308959147383955	19.307734682052878
25	20.878173154422626	28.53187299134242	20.79098963782303	29.798964216411918
26	23.43883711302806	37.25375177916031	21.407047010791256	17.900364097020372
27	31.05048885279284	27.624591881649753	21.727754122680786	19.597165142876623
28	23.401877937828818	27.263733225257848	27.841810819921747	21.492578016991587
29	27.223427836971908	28.717355264138288	23.99985718898244	20.05935970990736
30	24.157001985476708	27.624277963578788	24.80605455834007	23.412665492604432
31	23.336253208584274	28.44638282053526	26.80854958743046	21.408814383450007
32	22.391622712012367	28.26200645348149	27.61290533126104	21.733465503245096
33	22.172198794229455	29.12840109905636	28.55576813434684	20.14363197236734
34	24.640290041281713	29.546945212272867	24.963353324470212	20.84941142197521
35	20.708142474616356	27.710816762039393	24.975194030256485	26.605846733087766
36	21.774690987845123	30.838504953446854	24.28559679525182	23.101207263456207
37	21.319211771614107	32.47556215674761	24.755794273082106	21.44943179855618
38	24.99634872779244	28.010647805237664	23.441080637477356	23.55192282949254
39	23.22684425457499	30.35223490387421	23.30473317568639	23.116187665864413
40	20.690380786328895	30.050361264265785	25.223513476235777	24.03574447316954
41	21.100741081143422	31.455671474589654	24.353934417582103	23.08965302668482
42	21.134530042428448	31.626748785856822	23.6889013640101	23.54981980770463
43	22.481416521267835	29.64161968052777	24.442084347469237	23.434879450735156
44	22.544993851771807	28.61115251332116	23.723870030182212	25.119983604724823
45	23.357821995400833	28.26016266494798	28.070996999441167	20.31101834021002
46	23.421463908858687	29.210812791687772	24.059754901432438	23.307968398021103
47	21.990641840653137	30.228016392913617	25.49149698280035	22.289844783632905
48	24.70074038763703	27.89031271775549	24.53238076297728	22.876566131630206
49	21.458036312714057	29.09807841574672	24.045944819231043	25.39794045230818
50	20.482262217934213	29.138063871285684	28.522742259025062	21.856931651755037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57916.0
1	52823.5
2	32237.0
3	16313.5
4	15133.0
5	11308.5
6	6093.0
7	3980.5
8	3723.0
9	3364.5
10	3108.5
11	2845.0
12	2687.5
13	2667.0
14	3021.5
15	3653.5
16	4180.0
17	4704.0
18	5428.5
19	6271.0
20	7167.0
21	8245.0
22	9386.0
23	10716.0
24	11450.5
25	12100.5
26	13930.5
27	16487.5
28	18288.0
29	20226.5
30	23809.0
31	28065.0
32	33097.5
33	36905.5
34	41281.0
35	48208.5
36	53593.0
37	55124.0
38	55076.5
39	56078.5
40	58428.0
41	63511.0
42	69317.5
43	72569.0
44	74911.0
45	77314.0
46	81226.5
47	87361.0
48	116072.0
49	154106.5
50	189212.5
51	211522.0
52	189929.0
53	149542.5
54	119849.0
55	98208.0
56	84684.5
57	78555.0
58	72022.0
59	65520.0
60	59512.5
61	52727.5
62	44944.0
63	37278.5
64	30338.0
65	25290.0
66	19179.5
67	13718.0
68	10722.5
69	8192.0
70	6133.5
71	4518.0
72	3328.0
73	2534.0
74	1915.0
75	1490.0
76	1169.0
77	792.5
78	558.5
79	473.0
80	374.5
81	289.5
82	220.5
83	167.5
84	124.5
85	91.0
86	75.5
87	63.0
88	45.0
89	39.0
90	26.5
91	12.0
92	8.5
93	7.0
94	4.5
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	19286.0
25	14924.0
26	17732.0
27	29421.0
28	31649.0
29	22725.0
30	20204.0
31	19792.0
32	22481.0
33	15521.0
34	10557.0
35	9868.0
36	9228.0
37	7756.0
38	8915.0
39	10341.0
40	12527.0
41	10518.0
42	13866.0
43	20637.0
44	46338.0
45	99352.0
46	153065.0
47	116016.0
48	224536.0
49	146031.0
50	288142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.681526861139961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.95968045335877	9.708367741805365
2	11.109694256758994	3.039951607858011
3	4.1079581559264655	1.686094195642408
4	2.089546208073938	1.1435273029342696
5	1.2556050210594643	0.8589296911303634
6	0.8807790324288436	0.7230241194542454
7	0.7092425298921522	0.679246450664764
8	0.5124160529401968	0.5608507193904494
9	0.48116759838153134	0.5924796679770414
>10	4.544882869106809	14.5831734859262
>50	1.9167322376658624	18.856109464018704
>100	1.3576392508567863	30.039442890481656
>500	0.048111859398998126	4.393436204640164
>1k	0.019908355613378538	5.802909977737938
>5k	0.005530098781494038	5.331319982154792
>10k+	0.0011060197562988076	2.0011364981836413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGCAG	14398	1.0273806431725354	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTT	12051	0.8599086075060581	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT	9741	0.695076736014979	No Hit
AAGCAGTGGTATCAACGCAGAGTG	8708	0.6213662064694012	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	8120	0.5794090028171266	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	7710	0.5501531295221731	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	7657	0.5463712727303864	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	6176	0.4406933499259327	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5753	0.41050985138016366	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5716	0.4078696872047654	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	5519	0.39381259686548287	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	5364	0.38275244964422006	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4993	0.3562794521017134	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	4955	0.3535679321377909	No Hit
AAGCAGTGGTATCAACGCAGAGTGCA	4925	0.3514272584820626	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4016	0.28656484671349514	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTA	3929	0.280356893111883	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3297	0.2352600347645402	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3135	0.22370039702360733	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	3074	0.2193476939236265	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	3066	0.21877684761543226	No Hit
AAGCAGTGGTATCAACGCAGAGTGC	2866	0.20450568991057694	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGCATGGGAAGCAGTGGT	2783	0.19858315946306193	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTT	2599	0.18545369437459505	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2191	0.15634053265669018	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1987	0.14178395179773773	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC	1912	0.13643226765841698	Illumina Single End Adapter 2 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1898	0.1354332866190771	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	1724	0.12301737941585297	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG	1457	0.1039653838798711	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	1427	0.10182471022414281	Illumina Single End Adapter 2 (100% over 32bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.13935785498791234	0.0	0.0	0.0	0.0
2	0.32288494307235194	0.0	0.0	0.0	0.0
3	0.3709787445377144	0.0	0.0	0.0	0.0
4	0.4153620449998145	0.0	0.0	0.0	0.0
5	0.7289707355640104	0.0	0.0	0.0	0.0
6	0.7955456862571606	0.0	0.0	0.0	0.0
7	0.8628341948355535	0.0	0.0	0.0	0.0
8	0.942181831674549	0.0	0.0	0.0	0.0
9	0.9902042773513873	0.0	0.0	0.0	0.0
10	1.0402960408954296	0.0	0.0	0.0	0.0
11	1.1421207511195723	0.0	0.0	0.0	0.0
12	1.2014887671717704	0.0	0.0	0.0	0.0
13	1.2479413855010746	0.0	0.0	0.0	0.0
14	1.3148731151368462	0.0	0.0	0.0	0.0
15	1.4300413578150286	0.0	0.0	0.0	0.0
16	1.4694297530804294	0.0	0.0	0.0	0.0
17	1.7524981661562349	0.0	0.0	0.0	0.0
18	1.7936704561347425	0.0	0.0	0.0	0.0
19	2.1088489740464724	0.0	0.0	0.0	0.0
20	2.1433851756922224	0.0	0.0	0.0	0.0
21	2.2674015361474154	0.0	0.0	0.0	0.0
22	2.29701418838499	0.0	0.0	0.0	0.0
23	2.3502456066241004	0.0	0.0	0.0	0.0
24	2.4049041406336964	0.0	0.0	0.0	0.0
25	2.4358725528532323	0.0	0.0	0.0	0.0
26	2.8596545808989116	0.0	0.0	0.0	0.0
27	2.900256024569225	0.0	0.0	0.0	0.0
28	2.981387556121328	0.0	0.0	0.0	0.0
29	3.0672999255045568	0.0	0.0	0.0	0.0
30	3.1054039165765204	0.0	0.0	0.0	0.0
31	3.138513002451785	0.0	0.0	0.0	0.0
32	3.1701949725565637	0.0	0.0	0.0	0.0
33	3.200235759525284	0.0	0.0	0.0	0.0
34	3.226780112856315	0.0	0.0	0.0	0.0
35	3.2503988788578506	0.0	0.0	0.0	0.0
36	3.2720196827807064	0.0	0.0	0.0	0.0
37	3.2916425246248826	0.0	0.0	0.0	0.0
38	3.3040584318281065	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCAC	10	0.0034497147	184.47638	44
GGAATCG	60	0.0	138.35728	44
TGCTCGA	20	1.4815309E-4	138.35728	44
CGATATT	20	1.4815309E-4	138.35728	44
CTACCCT	70	0.0	131.76884	44
CCGCTCA	15	2.3698839E-4	122.97012	43
CACCGTG	15	2.3698839E-4	122.97012	43
TATCGTT	15	2.3698839E-4	122.97012	43
CTCGACA	20	4.833708E-6	122.970116	43
TCGTGCG	130	0.0	113.51089	43
TCGATTT	25	3.6013906E-4	110.68583	44
CCACTAC	60	1.2187229E-10	107.61122	44
TACTGGC	60	1.2187229E-10	107.61122	44
CTATCAT	605	0.0	106.72188	44
GATCCAA	35	1.0388701E-5	105.41508	44
CACGAAA	35	1.0388701E-5	105.41508	44
ACTGGGC	35	1.0388701E-5	105.41508	44
ATGCGGA	45	2.9352123E-7	102.486885	44
CTTATGT	40	2.0163352E-5	92.23819	44
ATGCACG	30	7.435583E-4	92.23819	44
>>END_MODULE