FastQCFastQC Report
Tue 24 May 2016
ERR841021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623310
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC308664.951950072997384No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA204333.2781441016508635No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT119141.9114084484445941No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT111801.7936500296802556No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC66391.0651200847090532No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA41220.6613081773114502No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA39680.6366013701047633No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC28900.4636537196579551No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG28690.46028460958431594No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA24950.4002823635109336No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA24560.39402544480274665No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG24380.39113763616819885No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT19550.31364810447449903No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT17270.27706919510356004No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG16520.26503665912627744No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC16110.25845887279202967No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA14020.22492820586866888No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA14010.22476777205563844No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT13520.21690651521714716No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT13360.2143395742086602No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC12480.20022139866198196No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT12090.19396447995379507No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT12050.19332274470167332No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT11460.1838571497328777No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC11180.17936500296802554No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT10980.17615632670741685No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10690.17150374612953426No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA10580.1697389741861995No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAAC9240.1482408432401213No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT8970.14390913028829955No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC8950.14358826266223867No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA8200.13155572668495613No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT7790.1249779403507083No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT7790.1249779403507083No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG7670.1230527345943431No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC7660.12289230078131266No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC7480.12000449214676484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA7340.11775841876433878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA7280.11679581588615617No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAG7270.11663538207312572No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA6840.10973672811281705No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT6740.1081323899825127No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG6360.1020359050873562No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG6290.10091286839614318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAC252.8412614E-9222.6950544
CGTGAGG100.0019621185222.6950444
GACGTAT152.218289E-5222.6950444
ATCAGGA152.218289E-5222.6950444
GAATCAT100.0019621185222.6950444
GTTAGCG152.218289E-5222.6950444
GACTTAG100.0019621185222.6950444
CCCAAGC100.0019621185222.6950444
CATAGGG152.218289E-5222.6950444
TTAAGCC152.218289E-5222.6950444
CAGGACG152.218289E-5222.6950444
GGAGGTA400.0222.6950444
GGAGGGT152.218289E-5222.6950444
CCATTGT152.218289E-5222.6950444
GACTGCG550.0202.4500644
TTTTCGG359.458745E-11190.8814744
GGAATAT308.449206E-9185.5792144
TTTAACG308.449206E-9185.5792144
AGGATAT308.449206E-9185.5792144
CACTGGT550.0182.2050544
Produced by FastQC (version 0.11.5)