##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841021.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623310
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.107710449054245	39.0	37.0	39.0	34.0	39.0
2	37.67301824132454	39.0	38.0	39.0	35.0	39.0
3	37.98145064253742	39.0	38.0	39.0	35.0	39.0
4	38.63578155332018	39.0	38.0	41.0	35.0	41.0
5	39.216463717893184	40.0	38.0	41.0	35.0	41.0
6	39.19525436781056	40.0	38.0	41.0	35.0	41.0
7	39.29424844780286	40.0	39.0	41.0	35.0	41.0
8	39.22180616386709	40.0	39.0	41.0	35.0	41.0
9	39.21359836999246	40.0	39.0	41.0	35.0	41.0
10	39.19965346296385	40.0	39.0	41.0	35.0	41.0
11	39.077200750830244	40.0	38.0	41.0	35.0	41.0
12	39.10013957741734	40.0	38.0	41.0	35.0	41.0
13	39.06057178610964	40.0	38.0	41.0	35.0	41.0
14	38.97806548908248	40.0	38.0	41.0	35.0	41.0
15	38.91129935345173	40.0	38.0	41.0	35.0	41.0
16	38.93799714427813	40.0	38.0	41.0	35.0	41.0
17	38.93177391667068	40.0	38.0	41.0	35.0	41.0
18	38.832304952591805	40.0	38.0	41.0	35.0	41.0
19	38.81652147406587	40.0	38.0	41.0	35.0	41.0
20	38.683000433171294	40.0	38.0	41.0	35.0	41.0
21	38.658319295374696	40.0	38.0	41.0	35.0	41.0
22	38.58418764338772	40.0	38.0	41.0	35.0	41.0
23	38.5041664661244	40.0	38.0	41.0	35.0	41.0
24	38.26449760151449	40.0	37.0	41.0	34.0	41.0
25	38.2213846414264	40.0	37.0	41.0	34.0	41.0
26	38.139208393004104	40.0	37.0	41.0	34.0	41.0
27	38.11733799494304	40.0	37.0	41.0	34.0	41.0
28	37.867626260060405	39.0	36.0	41.0	33.0	41.0
29	37.97957208228553	39.0	36.0	41.0	33.0	41.0
30	37.83818057358043	39.0	36.0	41.0	33.0	41.0
31	37.893698028590116	39.0	36.0	41.0	33.0	41.0
32	37.901203736160895	39.0	36.0	41.0	33.0	41.0
33	37.796630938796284	39.0	36.0	41.0	33.0	41.0
34	37.73798967640544	39.0	36.0	41.0	33.0	41.0
35	37.56036536460684	39.0	35.0	41.0	33.0	41.0
36	37.4499559981235	39.0	35.0	41.0	33.0	41.0
37	37.382535602185285	38.0	35.0	40.0	33.0	41.0
38	37.082869761990096	38.0	35.0	40.0	32.0	41.0
39	37.20614850258931	38.0	35.0	41.0	32.0	41.0
40	36.92202450131846	38.0	35.0	40.0	31.0	41.0
41	36.91004184100419	38.0	35.0	40.0	31.0	41.0
42	36.85296393249292	38.0	35.0	40.0	31.0	41.0
43	36.6900846441623	38.0	34.0	40.0	31.0	41.0
44	36.351960820414625	38.0	34.0	40.0	30.0	41.0
45	36.17177816064176	38.0	34.0	40.0	29.0	41.0
46	35.66808253357664	37.0	33.0	40.0	28.0	41.0
47	36.04425562686432	37.0	34.0	40.0	30.0	41.0
48	36.80492835466758	38.0	35.0	40.0	32.0	41.0
49	37.79020120235902	39.0	36.0	40.0	34.0	41.0
50	37.32142857142857	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	12.0
22	20.0
23	65.0
24	95.0
25	192.0
26	269.0
27	463.0
28	916.0
29	1813.0
30	3494.0
31	7002.0
32	12737.0
33	22176.0
34	33371.0
35	33649.0
36	55190.0
37	96372.0
38	99797.0
39	91457.0
40	164215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.083056585005856	14.72493622755932	42.19200718743482
2	23.27333108725995	22.647478782628227	37.068392934494874	17.01079719561695
3	21.353900948163833	33.942821389035956	19.087292037669858	25.61598562513035
4	18.729845502238053	36.677576165952736	22.08387479745231	22.5087035343569
5	14.417705475606038	38.051852208371436	24.35593845758932	23.174503858433205
6	15.98434165984823	33.304615680800886	20.500232629028893	30.210810030321987
7	19.698063563876723	28.72086120870835	18.985577000208565	32.59549822720636
8	18.33405528549197	29.57019781489147	32.18703373923088	19.908713160385684
9	17.34096998283358	39.75902841282829	26.980154337328134	15.919847267009995
10	35.50528629413935	21.037846336493878	23.09653302530041	20.360334344066356
11	18.028910173108084	27.338884343264187	20.935329129967432	33.6968763536603
12	13.858112335755884	29.039482761386793	23.51253790248833	33.589867000369
13	18.346087821469254	29.44874941842743	20.588310792382604	31.616851967720716
14	14.157481830870674	28.331648778296515	22.205644061542408	35.3052253292904
15	18.36277293802442	42.381158652997705	21.937238292342496	17.318830116635382
16	13.571256678057466	47.67483274774992	17.815372767964575	20.93853780622804
17	16.178627007428087	45.485071633697515	17.92414689319921	20.412154465675187
18	15.342766841539524	30.838747974523113	18.743803243971698	35.07468193996567
19	20.203750942548652	24.47690555261427	21.232612985512826	34.086730519324256
20	14.622900322471963	25.89096918066452	27.752964014695735	31.73316648216778
21	14.778841988737545	44.252137780558634	21.68134636055895	19.28767387014487
22	19.732556833678267	29.84149139272593	19.332755771606426	31.09319600198938
23	24.96606824854406	24.103255202066386	31.826699395164525	19.103977154225024
24	16.33504997513276	28.697598305818932	24.386902183504194	30.580449535544112
25	17.26908536929414	26.19388849033943	19.777528796317938	36.75949734404849
26	15.941167322235112	43.56333309573027	23.653924662549652	16.841574919484962
27	17.694295284518937	35.19537053932174	18.00432708599432	29.106007090165004
28	19.280091905950453	26.332496980443764	19.18848094606753	35.19893016753826
29	18.92699014717401	32.09712660263017	30.614008327493096	18.361874922702725
30	20.613160559757215	31.540790906514317	31.233166427163773	16.6128821065647
31	19.433970448872813	38.919662044019695	19.931828192362623	21.714539314744865
32	16.50763886908845	30.89880196636516	23.87630885551979	28.717250309026593
33	16.529435461442386	25.60607873840558	35.80360739683219	22.060878403319844
34	21.109346104604295	38.750397973215556	22.03010371012647	18.110152212053677
35	15.60751912732078	28.168800628012992	20.772449165515823	35.45123107915041
36	13.373317399061065	28.66765057373653	25.50259730920821	32.4564347179942
37	15.879840149588581	45.38846026036815	18.299290797237568	20.4324087928057
38	28.108265334212863	24.767608352767837	20.74825868579472	26.37586762722458
39	15.333980152972265	38.473168887859245	27.482576804025843	18.71027415514265
40	13.968525391413653	25.861742058489963	28.01166428488664	32.15806826520974
41	13.917452662931707	37.98046237855471	23.9390823345862	24.163002623927383
42	14.697531470843122	39.140347899114616	22.931679904225	23.23044072581726
43	14.841617194609352	24.155905591425807	22.181717727607577	38.82075948635727
44	17.08859594211437	25.2082336045955	19.738004934271093	37.96516551901904
45	14.638310478432718	25.828701285539434	37.57474293109228	21.958245304935566
46	14.650169882123057	32.94289288116138	32.0738762697347	20.333060966980874
47	14.548107591585852	38.81277359538229	26.202399245877505	20.43671956715435
48	16.471551318907267	37.280515219636676	19.110005404178164	27.137928057277893
49	16.85016207571149	28.216881577632126	18.99831974372522	35.93463660293117
50	16.43448377799128	30.876321040573494	35.1563077377873	17.532887443647923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15284.0
1	13161.0
2	8699.5
3	5987.0
4	5014.0
5	3736.5
6	2281.0
7	1481.0
8	1359.0
9	1236.0
10	1305.5
11	1529.0
12	1696.5
13	1715.5
14	1879.5
15	2200.0
16	2390.5
17	2498.0
18	2476.0
19	2215.5
20	1983.0
21	2061.0
22	2423.5
23	3380.0
24	4490.0
25	5269.5
26	6050.5
27	7223.0
28	8340.5
29	9438.5
30	11392.0
31	15507.0
32	22633.0
33	27108.5
34	31458.0
35	36954.5
36	35386.5
37	31997.0
38	31673.0
39	32468.0
40	34435.0
41	37171.0
42	38642.0
43	37679.5
44	36804.5
45	37847.5
46	39822.0
47	38333.0
48	63472.0
49	100103.5
50	91701.0
51	73967.0
52	64973.5
53	47382.0
54	35402.0
55	29483.0
56	24274.0
57	19750.5
58	16835.0
59	14574.0
60	11990.0
61	10093.0
62	8671.0
63	7320.0
64	6126.0
65	5049.0
66	3517.0
67	2039.0
68	1290.5
69	963.0
70	756.0
71	670.0
72	616.5
73	567.0
74	423.5
75	271.0
76	184.0
77	134.5
78	108.5
79	91.0
80	64.0
81	49.0
82	42.0
83	35.0
84	28.0
85	20.0
86	13.5
87	11.5
88	8.0
89	4.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3005.0
25	3029.0
26	3468.0
27	3619.0
28	3764.0
29	3092.0
30	3965.0
31	3141.0
32	2946.0
33	2789.0
34	3243.0
35	3183.0
36	3273.0
37	3212.0
38	3490.0
39	4489.0
40	5562.0
41	4395.0
42	5797.0
43	10708.0
44	30048.0
45	56599.0
46	69288.0
47	74484.0
48	102634.0
49	101839.0
50	108248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.611718085703743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.63230031639885	7.969389228473793
2	11.532669218121779	2.6782820747300704
3	4.301090125316053	1.4982913798912258
4	2.23413515343272	1.037685902680849
5	1.367837849040441	0.7941473745006496
6	0.9478149135775178	0.6603455744332676
7	0.7709631512773395	0.6266544736968763
8	0.5692416099036987	0.5287898477483115
9	0.5167387429708333	0.5400202146604418
>10	5.2986445970404965	14.789751488023617
>50	2.254859969327273	18.755996213762014
>100	1.4963317075866642	27.247597503649867
>500	0.03868632300316399	3.002197943238517
>1k	0.03177805103831328	6.870578043028348
>5k	0.0013816543929701425	1.0651200847090532
>10k+	0.00552661757188057	11.935152652773098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	30866	4.951950072997384	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	20433	3.2781441016508635	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	11914	1.9114084484445941	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11180	1.7936500296802556	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6639	1.0651200847090532	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4122	0.6613081773114502	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3968	0.6366013701047633	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2890	0.4636537196579551	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2869	0.46028460958431594	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2495	0.4002823635109336	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2456	0.39402544480274665	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	2438	0.39113763616819885	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	1955	0.31364810447449903	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1727	0.27706919510356004	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1652	0.26503665912627744	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1611	0.25845887279202967	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	1402	0.22492820586866888	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	1401	0.22476777205563844	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	1352	0.21690651521714716	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	1336	0.2143395742086602	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1248	0.20022139866198196	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	1209	0.19396447995379507	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	1205	0.19332274470167332	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1146	0.1838571497328777	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	1118	0.17936500296802554	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	1098	0.17615632670741685	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1069	0.17150374612953426	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	1058	0.1697389741861995	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAAC	924	0.1482408432401213	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	897	0.14390913028829955	No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC	895	0.14358826266223867	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	820	0.13155572668495613	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	779	0.1249779403507083	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	779	0.1249779403507083	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	767	0.1230527345943431	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC	766	0.12289230078131266	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	748	0.12000449214676484	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	734	0.11775841876433878	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	728	0.11679581588615617	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAG	727	0.11663538207312572	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	684	0.10973672811281705	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT	674	0.1081323899825127	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG	636	0.1020359050873562	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	629	0.10091286839614318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.09257031011856058	0.0	0.0	0.0	0.0
2	0.14663650510981693	0.0	0.0	0.0	0.0
3	0.1824132454156038	0.0	0.0	0.0	0.0
4	0.21578347852593413	0.0	0.0	0.0	0.0
5	0.25701496847475575	0.0	0.0	0.0	0.0
6	0.3012947008711556	0.0	0.0	0.0	0.0
7	0.3348253677945164	0.0	0.0	0.0	0.0
8	0.37862379875182495	0.0	0.0	0.0	0.0
9	0.4158444433748857	0.0	0.0	0.0	0.0
10	0.4500168455503682	0.0	0.0	0.0	0.0
11	0.47745102757857244	0.0	0.0	0.0	0.0
12	0.5172386132101202	0.0	0.0	0.0	0.0
13	0.5586305369719722	0.0	0.0	0.0	0.0
14	0.59472814490382	0.0	0.0	0.0	0.0
15	0.6264940398838459	0.0	0.0	0.0	0.0
16	0.6632333830678153	0.0	0.0	0.0	0.0
17	0.6986892557475414	0.0	0.0	0.0	0.0
18	0.7598145385121369	0.0	0.0	0.0	0.0
19	0.7902969629879193	0.0	0.0	0.0	0.0
20	0.8194959169594583	0.0	0.0	0.0	0.0
21	0.8557539587043366	0.0	0.0	0.0	0.0
22	0.8823859716673886	0.0	0.0	0.0	0.0
23	0.9066114774349842	0.0	0.0	0.0	0.0
24	0.9332434903980363	0.0	0.0	0.0	0.0
25	0.9590733342959362	0.0	0.0	0.0	0.0
26	0.987790986828384	0.0	0.0	0.0	0.0
27	1.0139416983523448	0.0	0.0	0.0	0.0
28	1.0423384832587317	0.0	0.0	0.0	0.0
29	1.0715374372302706	0.0	0.0	0.0	0.0
30	1.0984903178193837	0.0	0.0	0.0	0.0
31	1.1257640660345574	0.0	0.0	0.0	0.0
32	1.149989571802153	0.0	0.0	0.0	0.0
33	1.1754985480739921	0.0	0.0	0.0	0.0
34	1.2048579358585616	0.0	0.0	0.0	0.0
35	1.2269978020567616	0.0	0.0	0.0	0.0
36	1.2423994481076832	0.0	0.0	0.0	0.0
37	1.2680688581925528	0.0	0.0	0.0	0.0
38	1.277855320787409	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTAC	25	2.8412614E-9	222.69505	44
CGTGAGG	10	0.0019621185	222.69504	44
GACGTAT	15	2.218289E-5	222.69504	44
ATCAGGA	15	2.218289E-5	222.69504	44
GAATCAT	10	0.0019621185	222.69504	44
GTTAGCG	15	2.218289E-5	222.69504	44
GACTTAG	10	0.0019621185	222.69504	44
CCCAAGC	10	0.0019621185	222.69504	44
CATAGGG	15	2.218289E-5	222.69504	44
TTAAGCC	15	2.218289E-5	222.69504	44
CAGGACG	15	2.218289E-5	222.69504	44
GGAGGTA	40	0.0	222.69504	44
GGAGGGT	15	2.218289E-5	222.69504	44
CCATTGT	15	2.218289E-5	222.69504	44
GACTGCG	55	0.0	202.45006	44
TTTTCGG	35	9.458745E-11	190.88147	44
GGAATAT	30	8.449206E-9	185.57921	44
TTTAACG	30	8.449206E-9	185.57921	44
AGGATAT	30	8.449206E-9	185.57921	44
CACTGGT	55	0.0	182.20505	44
>>END_MODULE