FastQCFastQC Report
Tue 24 May 2016
ERR841020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4711994
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT920561.9536527423422019No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC801681.7013604007135832No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC527591.11967460060433No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA526321.1169793509923824No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA331960.7045000481749341No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT303540.6441858796933952No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA271510.5762104111338002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA220980.4689734324789039No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA208850.4432306153191197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA204690.4344020811571492No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC175370.37217789326556866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA172780.3666812818522264No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA167180.35479671663418927No Hit
AAAAAAAAAAAAAAAAAAAAAAAA140660.2985148113516274No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC133780.28391377408375307No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA123490.26207588549560973No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112010.23771252679863344No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG111820.23730930047873577No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACC110330.23414715723322227No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG107930.22905377213977776No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG98220.20844678494921684No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT94780.20114626631527968No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG94300.20012758929659077No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT89300.1895163703519147No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT89050.1889858094046809No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT86660.18391364674912575No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA85610.18168529077074377No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAA84620.1795842694196979No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC83050.17625234667106962No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC82570.1752336696523807No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG77600.16468611802137267No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA76870.16313688005545No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC75000.15916828417014114No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA73460.1559000287351809No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT72930.15477523952704522No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC72750.1543932356450369No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT71550.15184654309831463No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT69900.14834484084657154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64270.13639660831486627No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63630.13503837228994775No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT63620.13501714985205837No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT59320.12589150155963696No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC57400.12181679348488134No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT57230.12145601204076237No Hit
ATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAATTCC50560.10730064596856448No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG49460.10496617780073575No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG49100.10420217003671907No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA48870.10371405396526397No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC47870.10159181017632875No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA47390.10057313315763984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC3450.0149.8434144
CTATCAT139850.0137.0454444
CTATACG600.0127.6443844
ACTTCTA52200.0106.1869344
ACATTGG20300.092.4321444
CCAGAAT14900.089.9507444
TCGTGCG6450.088.7561443
CCTTAAC128500.087.9195443
AATGAAG20950.084.9947144
CCTATCA138600.084.67374443
TACGGAA703.4040568E-882.05710644
GTTCACG454.8888978E-877.8880543
TAATTCC15550.077.5716644
CCCGAGA505.0776704E-576.5866344
AATCGCA11450.074.4127744
TTAGACC400.002011679371.7999744
GACATTG16150.071.2567143
CCCTATC172800.070.8417642
GAACACA36900.070.82706544
ACGACAT404.472895E-968.37710642
Produced by FastQC (version 0.11.5)