##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841020.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4711994
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.17479691188062	39.0	37.0	39.0	34.0	39.0
2	37.73678192289719	39.0	38.0	39.0	35.0	39.0
3	37.99210206973948	39.0	38.0	39.0	35.0	39.0
4	38.44504428486114	39.0	38.0	40.0	35.0	41.0
5	39.24415863008314	40.0	39.0	41.0	35.0	41.0
6	39.17809466650424	40.0	38.0	41.0	35.0	41.0
7	39.23344172339778	40.0	38.0	41.0	35.0	41.0
8	39.18900851741322	40.0	38.0	41.0	35.0	41.0
9	39.20829589341582	40.0	39.0	41.0	35.0	41.0
10	39.17443188594892	40.0	39.0	41.0	35.0	41.0
11	39.0621927786835	40.0	38.0	41.0	35.0	41.0
12	39.03969105223818	40.0	38.0	41.0	35.0	41.0
13	38.89004379037834	40.0	38.0	41.0	35.0	41.0
14	38.80860395832423	40.0	38.0	41.0	35.0	41.0
15	38.728771938164606	40.0	38.0	41.0	35.0	41.0
16	38.66370203357644	40.0	38.0	41.0	35.0	41.0
17	38.63180173828744	40.0	38.0	41.0	35.0	41.0
18	38.566161162344436	40.0	38.0	41.0	35.0	41.0
19	38.569099408870215	40.0	38.0	41.0	35.0	41.0
20	38.396253687929146	40.0	37.0	41.0	35.0	41.0
21	38.35155414034907	40.0	37.0	41.0	35.0	41.0
22	38.30730854071546	40.0	37.0	41.0	34.0	41.0
23	38.20222839842326	40.0	37.0	41.0	34.0	41.0
24	37.966075508585114	39.0	36.0	41.0	34.0	41.0
25	37.90132052533501	39.0	36.0	41.0	33.0	41.0
26	37.82769211572322	39.0	36.0	41.0	33.0	41.0
27	37.74855857966276	39.0	36.0	41.0	33.0	41.0
28	37.573805226623925	39.0	36.0	41.0	33.0	41.0
29	37.61535317823021	39.0	36.0	41.0	33.0	41.0
30	37.57290515958354	39.0	35.0	41.0	33.0	41.0
31	37.540718234512234	39.0	35.0	41.0	33.0	41.0
32	37.5145627880548	39.0	35.0	41.0	33.0	41.0
33	37.34986579215226	38.0	35.0	40.0	33.0	41.0
34	37.22808537887469	38.0	35.0	40.0	33.0	41.0
35	37.06012163364691	38.0	35.0	40.0	32.0	41.0
36	37.04077903590035	38.0	35.0	40.0	32.0	41.0
37	36.915453025012475	38.0	35.0	40.0	32.0	41.0
38	36.652937807914924	38.0	35.0	40.0	31.0	41.0
39	36.661065723686384	38.0	35.0	40.0	31.0	41.0
40	36.40211111586414	37.0	34.0	40.0	31.0	41.0
41	36.32557744934249	37.0	34.0	40.0	31.0	41.0
42	36.191847424754535	37.0	34.0	40.0	31.0	41.0
43	36.10065329363159	37.0	34.0	40.0	31.0	41.0
44	35.71358981364408	36.0	34.0	40.0	29.0	41.0
45	35.474843272614095	36.0	34.0	39.0	29.0	41.0
46	35.101766171321266	36.0	33.0	39.0	28.0	41.0
47	35.22003452291911	36.0	33.0	39.0	29.0	41.0
48	36.03768101627437	37.0	34.0	39.0	31.0	41.0
49	36.88385861848588	37.0	35.0	40.0	33.0	41.0
50	36.75257940542941	38.0	35.0	39.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	25.0
21	62.0
22	181.0
23	371.0
24	702.0
25	1353.0
26	2275.0
27	4277.0
28	8651.0
29	16626.0
30	31872.0
31	59320.0
32	105492.0
33	183337.0
34	276313.0
35	307546.0
36	517403.0
37	807308.0
38	774121.0
39	685837.0
40	928918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.09573441731887	17.795353729228008	32.10891185345312
2	23.70947416316744	28.02700937225302	31.192272316136226	17.071244148443313
3	25.863148382616785	28.325693963107764	20.771864310523316	25.039293343752135
4	20.70843044367204	32.97434164814301	23.66826018878632	22.648967719398623
5	15.622727872743472	32.18979056424944	22.51240133158064	29.675080231426442
6	18.122264162475588	32.2634112012876	23.61316674002556	26.00115789621124
7	20.04675727515782	28.253113225526178	21.515731980982995	30.184397518333
8	17.356410046362537	29.69995292863276	27.486028208015544	25.457608816989158
9	20.00059847274848	33.26254235468042	26.660496596557635	20.07636257601347
10	28.407506461171216	24.24867264262221	23.98146092715738	23.362359969049194
11	19.153568531708657	27.714890978214317	23.42583628077625	29.705704209300777
12	18.578440464907214	30.641252938777086	24.275752473369025	26.50455412294668
13	21.779441994196088	29.586476553238395	21.239521951853078	27.39455950071244
14	16.420840094448337	28.57603384045056	22.035957600964686	32.967168464136414
15	19.99898556746889	35.5843619495271	23.530547789322313	20.8861046936817
16	17.00933829712007	36.803018000447366	21.08585027909628	25.10179342333628
17	17.958830168289687	41.01715749213602	16.986757623205804	24.037254716368484
18	18.17763350292891	34.2376072635067	21.166028649442254	26.418730584122134
19	23.18211780405493	28.52781646156595	22.580164575761344	25.709901158617775
20	19.16889113186477	27.900905646314488	27.718244123400837	25.211959098419907
21	17.756898671772504	38.8953169295207	21.70531201864858	21.64247238005821
22	23.678637960914212	31.081851971797924	19.548178541823273	25.69133152546459
23	24.98963283909105	28.267035144781595	25.569429842228153	21.173902173899204
24	19.078589658645576	31.424891457841415	24.148672515287583	25.347846368225426
25	17.936502944971085	29.04509689535678	22.718430900408357	30.299969259263776
26	18.89796025867868	36.57832335525833	26.061708629847523	18.462007756215463
27	21.55248942464023	34.298824310524054	18.68216861509791	25.466517649737803
28	20.861016660418173	27.957179541100928	20.869732922690726	30.312070875790177
29	20.25815552497687	34.92088044292221	24.158254738301142	20.66270929379978
30	22.098265352651218	33.80097593213149	24.553287566839224	19.54747114837806
31	22.962868926737034	33.43736154136483	21.380338007727246	22.219431524170886
32	18.311853939355675	34.64222374002032	22.66091178850823	24.38501053211578
33	17.81167348205041	27.515833659480787	29.255900648973714	25.416592209495086
34	20.958575462432478	34.0113878182711	23.088466718254736	21.941570001041683
35	17.171796587135173	29.11106727663664	22.716233629637223	31.000902506590965
36	16.88327736824588	28.862214786363694	27.611415088965895	26.64309275642453
37	15.630146612457654	40.192326451137546	20.14529132608463	24.032235610320168
38	21.918587782640948	28.45904496290371	21.626233863126053	27.996133391329288
39	18.336865833856297	33.14930132237451	27.46136022923779	21.0524726145314
40	15.527475658678444	29.268362239370166	30.316573124744878	24.887588977206512
41	16.9738331689041	34.203599767284416	22.44128216227249	26.381284901538994
42	16.727777359042683	34.64175734744618	23.05959388607351	25.57087140743762
43	17.510378575220017	27.014612904263302	21.870589370434168	33.604419150082514
44	16.726032010267684	29.864702929721005	20.335402542776723	33.07386251723459
45	16.222799263489392	27.59200109761221	32.57196273457057	23.613236904327827
46	16.31042253068865	33.23115428332893	27.530455253752407	22.92796793223002
47	17.816665501306264	32.32632068881981	28.348168724410627	21.508845085463292
48	16.03432323508605	31.641737621162928	22.704805658125114	29.61913348562591
49	16.583836265926198	32.65538964289424	21.08091941429997	29.67985467687958
50	16.26810045362673	29.759345416211865	30.11378020892562	23.85877392123579
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	225807.0
1	210270.0
2	129285.0
3	60286.0
4	52249.5
5	38370.5
6	20346.0
7	11532.5
8	10524.0
9	8846.5
10	7099.0
11	6370.0
12	6646.0
13	6946.0
14	7628.0
15	8585.5
16	9577.5
17	10641.5
18	11807.0
19	13462.5
20	15699.5
21	19155.0
22	23281.5
23	28447.0
24	33355.5
25	38206.5
26	44132.5
27	48351.0
28	52150.0
29	58905.5
30	70462.5
31	101420.0
32	159989.5
33	192954.5
34	197756.0
35	206905.0
36	201554.0
37	193375.0
38	192451.5
39	196876.5
40	213178.5
41	248197.5
42	280895.5
43	298427.0
44	311271.0
45	321493.5
46	331946.0
47	334224.5
48	406960.5
49	536960.5
50	556659.0
51	508295.0
52	478637.0
53	396429.5
54	306384.0
55	271444.0
56	248480.5
57	223371.5
58	197479.5
59	169705.5
60	146660.5
61	128231.0
62	109412.5
63	90554.5
64	73231.0
65	59848.0
66	45400.0
67	31750.0
68	22633.5
69	16198.0
70	11790.0
71	8618.0
72	6557.5
73	4872.0
74	3458.0
75	2402.5
76	1596.5
77	957.5
78	629.5
79	499.0
80	379.5
81	258.0
82	190.0
83	165.5
84	155.0
85	125.5
86	99.5
87	74.5
88	45.5
89	33.0
90	38.0
91	41.5
92	35.5
93	35.0
94	19.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	37415.0
25	42423.0
26	47442.0
27	52955.0
28	51514.0
29	43666.0
30	48987.0
31	31867.0
32	29972.0
33	25017.0
34	27083.0
35	25171.0
36	24830.0
37	25658.0
38	26256.0
39	34021.0
40	41730.0
41	36141.0
42	48939.0
43	76232.0
44	188361.0
45	382937.0
46	531635.0
47	462618.0
48	780939.0
49	624397.0
50	963788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.458328749606274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77930599864942	7.506915795524613
2	12.072835589776833	2.5252336707566574
3	4.295644957651565	1.3477580147612578
4	1.9803787641740938	0.8284580865788668
5	1.2199109137766835	0.6379114690754575
6	0.8218527002461842	0.5157123433755729
7	0.5871931151250515	0.42987410462382347
8	0.46248139813219413	0.3869426001795225
9	0.37918433804739476	0.35690710176013435
>10	3.3175306680095655	7.724629667200313
>50	1.3744723302325197	10.620353257354795
>100	1.5680977435668912	27.828002420871822
>500	0.08022288786511497	5.909257413555812
>1k	0.04790287548780246	10.544174451183931
>5k	0.007214288477078684	5.716801082536489
>10k+	0.005771430781662947	17.121068520660938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	92056	1.9536527423422019	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	80168	1.7013604007135832	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	52759	1.11967460060433	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	52632	1.1169793509923824	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	33196	0.7045000481749341	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	30354	0.6441858796933952	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	27151	0.5762104111338002	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	22098	0.4689734324789039	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	20885	0.4432306153191197	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	20469	0.4344020811571492	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	17537	0.37217789326556866	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	17278	0.3666812818522264	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16718	0.35479671663418927	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	14066	0.2985148113516274	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	13378	0.28391377408375307	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	12349	0.26207588549560973	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11201	0.23771252679863344	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	11182	0.23730930047873577	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACC	11033	0.23414715723322227	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	10793	0.22905377213977776	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	9822	0.20844678494921684	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	9478	0.20114626631527968	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	9430	0.20012758929659077	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	8930	0.1895163703519147	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	8905	0.1889858094046809	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	8666	0.18391364674912575	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	8561	0.18168529077074377	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAA	8462	0.1795842694196979	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	8305	0.17625234667106962	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	8257	0.1752336696523807	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	7760	0.16468611802137267	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	7687	0.16313688005545	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	7500	0.15916828417014114	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	7346	0.1559000287351809	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	7293	0.15477523952704522	No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC	7275	0.1543932356450369	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	7155	0.15184654309831463	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	6990	0.14834484084657154	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6427	0.13639660831486627	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6363	0.13503837228994775	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	6362	0.13501714985205837	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT	5932	0.12589150155963696	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	5740	0.12181679348488134	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	5723	0.12145601204076237	No Hit
ATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAATTCC	5056	0.10730064596856448	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	4946	0.10496617780073575	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	4910	0.10420217003671907	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA	4887	0.10371405396526397	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC	4787	0.10159181017632875	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	4739	0.10057313315763984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08609943051710167	0.0	0.0	0.0	0.0
2	0.1640706673225815	0.0	0.0	0.0	0.0
3	0.21967345459268411	0.0	0.0	0.0	0.0
4	0.2703738587103464	0.0	0.0	0.0	0.0
5	0.32309039442749715	0.0	0.0	0.0	0.0
6	0.39312443946235925	0.0	0.0	0.0	0.0
7	0.44236049536565625	0.0	0.0	0.0	0.0
8	0.500318973241477	0.0	0.0	0.0	0.0
9	0.5475601199831749	0.0	0.0	0.0	0.0
10	0.595119603293213	0.0	0.0	0.0	0.0
11	0.6486001467743805	0.0	0.0	0.0	0.0
12	0.7101239942156123	0.0	0.0	0.0	0.0
13	0.7637743171998945	0.0	0.0	0.0	0.0
14	0.8096360054787846	0.0	0.0	0.0	0.0
15	0.8549034654967727	0.0	0.0	0.0	0.0
16	0.8944196448467464	0.0	0.0	0.0	0.0
17	0.9506378828156402	0.0	0.0	0.0	0.0
18	1.0140505272290246	0.0	0.0	0.0	0.0
19	1.0541397124020107	0.0	0.0	0.0	0.0
20	1.0848273575900138	0.0	0.0	0.0	0.0
21	1.1178070260700672	0.0	0.0	0.0	0.0
22	1.1434012861646259	0.0	0.0	0.0	0.0
23	1.1735583704053953	0.0	0.0	0.0	0.0
24	1.2039489014629476	0.0	0.0	0.0	0.0
25	1.2372893513871197	0.0	0.0	0.0	0.0
26	1.2842333839983666	0.0	0.0	0.0	0.0
27	1.315048363813706	0.0	0.0	0.0	0.0
28	1.3515297345455024	0.0	0.0	0.0	0.0
29	1.3890297822959876	0.0	0.0	0.0	0.0
30	1.4277182865682767	0.0	0.0	0.0	0.0
31	1.464751440685196	0.0	0.0	0.0	0.0
32	1.500787140221316	0.0	0.0	0.0	0.0
33	1.5358678300524151	0.0	0.0	0.0	0.0
34	1.5706938506288421	0.0	0.0	0.0	0.0
35	1.5996412559099185	0.0	0.0	0.0	0.0
36	1.62546896282126	0.0	0.0	0.0	0.0
37	1.6491319810678875	0.0	0.0	0.0	0.0
38	1.663733018335762	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	345	0.0	149.84341	44
CTATCAT	13985	0.0	137.04544	44
CTATACG	60	0.0	127.64438	44
ACTTCTA	5220	0.0	106.18693	44
ACATTGG	2030	0.0	92.43214	44
CCAGAAT	1490	0.0	89.95074	44
TCGTGCG	645	0.0	88.75614	43
CCTTAAC	12850	0.0	87.91954	43
AATGAAG	2095	0.0	84.99471	44
CCTATCA	13860	0.0	84.673744	43
TACGGAA	70	3.4040568E-8	82.057106	44
GTTCACG	45	4.8888978E-8	77.88805	43
TAATTCC	1555	0.0	77.57166	44
CCCGAGA	50	5.0776704E-5	76.58663	44
AATCGCA	1145	0.0	74.41277	44
TTAGACC	40	0.0020116793	71.79997	44
GACATTG	1615	0.0	71.25671	43
CCCTATC	17280	0.0	70.84176	42
GAACACA	3690	0.0	70.827065	44
ACGACAT	40	4.472895E-9	68.377106	42
>>END_MODULE