FastQCFastQC Report
Tue 24 May 2016
ERR841019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612784
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC101250.6277964067103841No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA67730.4199570432246351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA61780.3830643161142472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA59840.37103542693875935No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG55190.34220329566761576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA53590.3322825623270072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA48510.30078423397057513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43790.2715180706157799No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT38780.24045377434299942No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT37140.23028502266887568No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA35090.217574083076221No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC34730.21534191807458408No Hit
AAAAAAAAAAAAAAAAAAAAAAAA33590.2082733955694005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32440.2011428684808381No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC26910.16685433387235984No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG26460.1640641276203137No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT24870.15420539886308396No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA23350.14478070218950584No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC23050.14292056468814174No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA22640.14037837676961082No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA22590.1400683538527168No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT20450.12679937300965288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19020.117932717586484No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT17560.10888004841317869No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG16460.10205954424151033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA100.0038443704177.922344
CGTCATG207.6850483E-7177.922344
GCCGCAT600.0148.268644
GCCCCGT252.3343455E-6142.3378644
TCGCGTT252.3343455E-6142.3378644
GATCGTT152.5425619E-4120.8198543
CTATCAT6600.0114.5711944
TTTGTCG254.1595523E-4106.7533944
AACGACA1400.0103.5598643
TCGTCCC303.2664502E-7100.68320543
AATCGTC453.6428173E-798.84572644
AATCCGG251.5996293E-596.65587643
CGCTACG506.8217196E-788.9611644
TTAGAGT6900.086.3825744
TTCGACA653.38041E-882.1179944
TGGCCGA453.8688086E-880.5465643
GCCACGG1050.080.5465643
ACGCTTA1450.079.7582844
AAATCCG204.7591755E-577.5925742
TACGGTC204.7591755E-577.5925742
Produced by FastQC (version 0.11.5)