FastQCFastQC Report
Tue 24 May 2016
ERR841018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988933
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC316263.1979921794499733No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA209872.122186235063447No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT124731.2612583461164708No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT90540.91553219479985No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC52350.5293584095181372No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA41100.4155994389913169No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA36780.3719159943090179No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC34730.35118658190190843No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA33560.3393556489671191No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA33460.3383444581179918No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG31640.3199407846638751No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA28820.2914252027184855No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA25770.2605838818201031No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC24490.24764063895127375No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG21330.21568700811885133No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT20040.20264264616510927No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG19340.19556431022121823No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG18490.18696918800363624No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG17720.17918301846535611No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG17080.17271139703094143No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT16750.16937446722882138No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC15140.15309429455787196No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA15130.15299317547295924No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA14430.1459148395290682No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT14160.1431846242364245No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA14090.14247679064203542No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC13680.13833090816061352No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT13290.13438726384901709No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT13060.1320615248960243No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA12200.12336528359352958No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT12140.12275856908405322No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG12040.12174737823492593No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG11990.12124178281036227No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC11950.12083730647071136No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC11760.11891604385736951No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.11487128046086034No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11050.11173658882856574No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.11133211248891484No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG10920.11042204072470026No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA10650.10769182543205658No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT10610.10728734909240566No Hit
TGCTCAAACTTGCCGAAGCCATGAATGCCCTCGAACTAGGCCCTTGGATG10450.105669443733802No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGT10430.10546720556397654No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT10360.10475937196958741No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG10250.10364706203554741No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.101928037592031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG305.456968E-12292.795544
CGAGTCG157.445704E-6292.795544
CTATACG600.0292.795544
CGCTGTT108.648788E-4292.795544
GTTACGC108.648788E-4292.795544
ATATCGT400.0256.1960844
CAGGTAC251.9474828E-7234.2363944
CTATCAT12400.0200.706644
CCGTCTC950.0200.3337644
TACCACG150.0029115102195.19744
ATATGGG150.0029115102195.19744
CCGAAGG150.0029115102195.19744
CGGACTT150.0029115102195.19744
CATAACC551.8189894E-12186.3244244
ACTTCTA5650.0183.9688644
GTTAGCA409.19681E-9182.997244
GAACACA8550.0171.2254344
TATTGTT451.8590072E-8162.6641744
TTACTTG553.2923708E-10159.7066344
GGCAAAT1150.0152.7628844
Produced by FastQC (version 0.11.5)