##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841018.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988933
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.327484268398365	38.0	35.0	39.0	25.0	39.0
2	36.39296089826105	38.0	35.0	39.0	32.0	39.0
3	36.7190183763713	38.0	35.0	39.0	33.0	39.0
4	37.26905968351749	39.0	35.0	40.0	33.0	41.0
5	37.80103505495317	39.0	35.0	41.0	34.0	41.0
6	37.744585325800635	39.0	35.0	41.0	34.0	41.0
7	37.88878114088619	39.0	35.0	41.0	34.0	41.0
8	37.785515297800764	39.0	35.0	41.0	34.0	41.0
9	37.7713960399744	39.0	35.0	41.0	34.0	41.0
10	37.74402815964277	39.0	35.0	41.0	34.0	41.0
11	37.58196055748974	39.0	35.0	41.0	34.0	41.0
12	37.66454754771051	39.0	35.0	41.0	34.0	41.0
13	37.6510097246224	39.0	35.0	41.0	34.0	41.0
14	37.566302267191006	39.0	35.0	40.0	34.0	41.0
15	37.37665241224633	38.0	35.0	40.0	33.0	41.0
16	37.47055260568714	39.0	35.0	40.0	33.0	41.0
17	37.5349027689439	39.0	35.0	40.0	34.0	41.0
18	37.41005103480216	38.0	35.0	40.0	33.0	41.0
19	37.480382391931506	39.0	35.0	40.0	33.0	41.0
20	37.416502432419584	38.0	35.0	41.0	33.0	41.0
21	37.31757459807692	38.0	35.0	40.0	33.0	41.0
22	37.182162997897734	38.0	35.0	40.0	33.0	41.0
23	37.1471545595101	38.0	35.0	40.0	33.0	41.0
24	36.9406117502399	38.0	35.0	40.0	33.0	41.0
25	36.92726605661229	38.0	35.0	40.0	33.0	41.0
26	36.79765324569283	38.0	35.0	40.0	33.0	41.0
27	36.6222349243816	38.0	35.0	40.0	32.0	41.0
28	36.51275039587358	37.0	35.0	40.0	32.0	41.0
29	36.416832045122206	37.0	35.0	40.0	32.0	41.0
30	36.38128738912643	37.0	34.0	40.0	32.0	41.0
31	36.32139777226461	37.0	34.0	40.0	32.0	41.0
32	36.31405268835944	37.0	34.0	40.0	32.0	41.0
33	36.0266174745052	36.0	34.0	39.0	31.0	41.0
34	35.98253941217251	36.0	34.0	39.0	31.0	41.0
35	35.788797994126966	36.0	34.0	39.0	31.0	41.0
36	35.78514967297649	36.0	34.0	39.0	31.0	41.0
37	35.62245027617289	36.0	34.0	39.0	31.0	41.0
38	35.45087462681922	35.0	34.0	39.0	31.0	40.0
39	35.3110810402769	35.0	34.0	38.0	31.0	40.0
40	35.11662194159432	35.0	34.0	38.0	30.0	40.0
41	34.926371195674434	35.0	33.0	38.0	30.0	40.0
42	34.845368074403126	35.0	33.0	37.0	30.0	40.0
43	34.44128130454679	35.0	33.0	37.0	29.0	40.0
44	34.156290507758975	35.0	32.0	37.0	29.0	39.0
45	33.948929886837284	35.0	32.0	37.0	28.0	39.0
46	33.80338488406163	35.0	32.0	36.0	28.0	39.0
47	33.882700134865146	35.0	32.0	36.0	29.0	39.0
48	34.59067656264902	35.0	34.0	37.0	30.0	39.0
49	35.082775741419525	35.0	34.0	37.0	31.0	39.0
50	35.35894736072418	36.0	34.0	38.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	14.0
22	36.0
23	89.0
24	218.0
25	534.0
26	1095.0
27	2087.0
28	4328.0
29	8116.0
30	15085.0
31	28339.0
32	51893.0
33	87221.0
34	136260.0
35	70266.0
36	102545.0
37	146355.0
38	134721.0
39	133902.0
40	65823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.904760989874944	18.07999126331106	37.01524774681399
2	26.81212984094979	20.56256591700348	34.1062539120446	18.519050330002134
3	25.089667348546364	26.440416084810597	22.417898886982233	26.052017679660803
4	18.87134922183808	29.85652213041733	27.87377911344853	23.398349534296052
5	14.64972854581655	32.04949172491969	29.311186905482977	23.989592823780782
6	21.570723193583387	27.535434655330544	24.638069515326112	26.255772635759953
7	23.05666814637594	24.83292599195294	23.268714867437936	28.841690994233176
8	21.533915846675153	23.285601754618362	31.772324313173893	23.40815808553259
9	20.02683700513584	33.02144836910084	25.397676081190536	21.554038544572787
10	33.64262290771973	21.453627293254446	25.105745283047487	19.79800451597833
11	22.598295334466542	23.120777646210612	24.395990426045042	29.884936593277807
12	20.218963266470023	25.36258775872582	26.15182221646967	28.26662675833449
13	22.603149050542353	24.638878468005416	23.734064896206316	29.02390758524592
14	17.55073397287784	26.249806609750102	24.11629503717643	32.08316438019562
15	19.23022085419336	34.53732457102756	26.545377694950012	19.687076879829068
16	20.065161138317762	37.40647748634134	19.78131986696773	22.747041508373165
17	19.87910202207834	39.949015757387	19.017365180452064	21.15451704008259
18	19.25843307888401	26.814556698987698	21.91098891431472	32.016021307813574
19	22.083801430430576	22.417494410642583	24.226818196986045	31.2718859619408
20	20.572475587324927	23.285601754618362	26.879980746926236	29.261941911130478
21	20.823149798823582	36.416117168706066	23.101565020077196	19.659168012393156
22	23.07557741525462	26.894946371493315	20.96936799560739	29.060108217644675
23	25.930068063256055	23.063847601404746	28.956157798354386	22.04992653698481
24	19.70487383877371	27.32561255413663	23.52191705605941	29.44759655103025
25	21.666009455707368	23.346369146587858	25.60886868925033	29.378752708454442
26	21.630832778937663	34.92437225548596	25.183665919637665	18.261129045938706
27	24.165195694927487	26.32122733015802	21.96598585605415	27.547591118860343
28	22.162180099406527	24.427914713223682	22.942416596928624	30.46748859044117
29	23.447044077710466	28.004491330687276	28.102987257154794	20.445477334447464
30	24.877922170720865	27.10027983065149	29.74590368067151	18.27589431795613
31	22.121234005675543	34.04592686366803	22.31102057808969	21.52181855256674
32	20.888874157598906	27.916127162871163	23.637590307856637	27.557408371673297
33	20.51490000517661	24.963763739582078	33.574512967404615	20.946823287836693
34	22.013533203216117	33.2832826859839	23.55888310389234	21.14430100690764
35	19.08516988438492	26.60697534484133	22.860269233415345	31.447585537358403
36	20.130370077486177	25.810639384503865	24.731214682284715	29.327775855725246
37	17.472092671225536	35.506917305029894	21.84124837439741	25.179741649347164
38	26.924145657765163	26.86279173678279	23.322714955462835	22.890347649989216
39	19.678478167269635	33.78316567600665	26.493287183300996	20.045068973422726
40	19.23486300597587	27.253692637275904	24.903033036418986	28.608411320329235
41	19.82959898414779	33.98781159400501	22.49586968691544	23.686719734931753
42	19.401667957430085	35.24611449569452	22.606591396391533	22.745626150483865
43	19.795966608483422	23.32690173629831	24.306313250991813	32.57081840422645
44	18.577926026634618	26.220175835781472	21.622257718053454	33.57964041953046
45	17.651126303244272	25.806898551245226	33.53662682473441	23.00534832077609
46	18.773516212225385	28.265435483262696	31.27101410174959	21.69003420276233
47	19.866619927231536	33.29286803419807	25.740419998722146	21.10009203984825
48	24.98916181838676	27.000945089436673	22.039659074159175	25.97023401801739
49	15.204931689436854	29.603465511496168	22.538487170943018	32.653115628123956
50	18.617880828598796	32.12831143381519	27.820796864603214	21.433010872982795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15407.0
1	13269.5
2	12391.0
3	12932.0
4	10975.0
5	7009.0
6	3394.0
7	2604.5
8	2544.5
9	2173.5
10	1825.5
11	1570.5
12	1332.0
13	1158.5
14	1136.0
15	1160.0
16	1123.5
17	1097.0
18	1151.5
19	1367.5
20	1588.5
21	1895.5
22	2287.0
23	2549.5
24	3029.5
25	3830.5
26	5206.5
27	6935.5
28	7883.0
29	8590.5
30	10244.5
31	14687.5
32	22865.0
33	29036.5
34	36957.5
35	45692.5
36	43444.5
37	38457.0
38	38278.5
39	42034.5
40	48051.5
41	51327.0
42	55656.5
43	63404.5
44	68444.5
45	68440.0
46	66946.0
47	69271.0
48	87261.5
49	109767.5
50	132226.5
51	146427.0
52	123592.5
53	93687.0
54	77261.0
55	65443.5
56	59807.0
57	55422.5
58	50009.5
59	44111.0
60	38248.0
61	31394.0
62	25288.0
63	19806.0
64	15527.5
65	13055.0
66	10086.5
67	6872.5
68	4664.5
69	3597.0
70	2801.5
71	1998.5
72	1486.0
73	1167.0
74	898.5
75	626.5
76	455.5
77	307.0
78	139.5
79	60.5
80	41.0
81	27.0
82	15.5
83	12.5
84	10.0
85	4.5
86	2.0
87	2.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	4977.0
25	5413.0
26	5830.0
27	8390.0
28	6923.0
29	5753.0
30	10063.0
31	6311.0
32	8025.0
33	5515.0
34	6022.0
35	5226.0
36	5436.0
37	5351.0
38	5513.0
39	7285.0
40	8032.0
41	8582.0
42	11505.0
43	21750.0
44	57534.0
45	90079.0
46	110321.0
47	117765.0
48	161232.0
49	163339.0
50	136761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.161095752874155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.47962268696132	7.419854344241203
2	11.520250666058306	2.5715724156197806
3	4.525171372566626	1.5151761296214323
4	2.4148413952912744	1.0780910416340057
5	1.546712966839244	0.8631505762552436
6	1.161129075375746	0.7775683675028959
7	0.8628865684134969	0.6741531729752424
8	0.6664862443496132	0.5950973432927611
9	0.5773788932053147	0.5799763001477716
>10	6.10657731663675	16.34721279552749
>50	2.4804560627013994	19.792261823377086
>100	1.5702887171133864	28.789962268356113
>500	0.046372162462526165	3.600115306282634
>1k	0.03727958158752104	7.340581830848253
>5k	0.0018185161750010263	1.4500929559259352
>10k+	0.0027277742625015393	6.60513332839215
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	31626	3.1979921794499733	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	20987	2.122186235063447	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	12473	1.2612583461164708	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	9054	0.91553219479985	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5235	0.5293584095181372	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	4110	0.4155994389913169	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3678	0.3719159943090179	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3473	0.35118658190190843	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3356	0.3393556489671191	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3346	0.3383444581179918	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	3164	0.3199407846638751	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	2882	0.2914252027184855	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2577	0.2605838818201031	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2449	0.24764063895127375	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	2133	0.21568700811885133	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2004	0.20264264616510927	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	1934	0.19556431022121823	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	1849	0.18696918800363624	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	1772	0.17918301846535611	No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG	1708	0.17271139703094143	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1675	0.16937446722882138	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1514	0.15309429455787196	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	1513	0.15299317547295924	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1443	0.1459148395290682	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1416	0.1431846242364245	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1409	0.14247679064203542	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	1368	0.13833090816061352	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1329	0.13438726384901709	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1306	0.1320615248960243	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	1220	0.12336528359352958	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	1214	0.12275856908405322	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	1204	0.12174737823492593	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1199	0.12124178281036227	No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC	1195	0.12083730647071136	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1176	0.11891604385736951	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.11487128046086034	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1105	0.11173658882856574	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.11133211248891484	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1092	0.11042204072470026	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	1065	0.10769182543205658	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT	1061	0.10728734909240566	No Hit
TGCTCAAACTTGCCGAAGCCATGAATGCCCTCGAACTAGGCCCTTGGATG	1045	0.105669443733802	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGT	1043	0.10546720556397654	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	1036	0.10475937196958741	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1025	0.10364706203554741	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1008	0.101928037592031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05146961422057915	0.0	0.0	0.0	0.0
2	0.07786169538280147	0.0	0.0	0.0	0.0
3	0.10081572765799099	0.0	0.0	0.0	0.0
4	0.12215185457457685	0.0	0.0	0.0	0.0
5	0.1431846242364245	0.0	0.0	0.0	0.0
6	0.17483489781410874	0.0	0.0	0.0	0.0
7	0.19940683544790194	0.0	0.0	0.0	0.0
8	0.22782129830837883	0.0	0.0	0.0	0.0
9	0.25107868783830656	0.0	0.0	0.0	0.0
10	0.27019019488681234	0.0	0.0	0.0	0.0
11	0.28899834468058	0.0	0.0	0.0	0.0
12	0.31276132963507136	0.0	0.0	0.0	0.0
13	0.33601871916499904	0.0	0.0	0.0	0.0
14	0.354321273534203	0.0	0.0	0.0	0.0
15	0.3743428523469234	0.0	0.0	0.0	0.0
16	0.4013416480186221	0.0	0.0	0.0	0.0
17	0.4238912039541607	0.0	0.0	0.0	0.0
18	0.45554147753184493	0.0	0.0	0.0	0.0
19	0.47536081817473985	0.0	0.0	0.0	0.0
20	0.497708135940453	0.0	0.0	0.0	0.0
21	0.5228867880837226	0.0	0.0	0.0	0.0
22	0.5427061287266175	0.0	0.0	0.0	0.0
23	0.5665702327660216	0.0	0.0	0.0	0.0
24	0.5853783825597891	0.0	0.0	0.0	0.0
25	0.6057033186272478	0.0	0.0	0.0	0.0
26	0.6304774944308664	0.0	0.0	0.0	0.0
27	0.6485778106302449	0.0	0.0	0.0	0.0
28	0.6727452719243872	0.0	0.0	0.0	0.0
29	0.7012608538697768	0.0	0.0	0.0	0.0
30	0.7314954602586828	0.0	0.0	0.0	0.0
31	0.7580897795907307	0.0	0.0	0.0	0.0
32	0.7809426927810074	0.0	0.0	0.0	0.0
33	0.8039978441411096	0.0	0.0	0.0	0.0
34	0.8282664245201646	0.0	0.0	0.0	0.0
35	0.8497036705216633	0.0	0.0	0.0	0.0
36	0.866590557702089	0.0	0.0	0.0	0.0
37	0.8834774448825148	0.0	0.0	0.0	0.0
38	0.8954094969022168	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	5.456968E-12	292.7955	44
CGAGTCG	15	7.445704E-6	292.7955	44
CTATACG	60	0.0	292.7955	44
CGCTGTT	10	8.648788E-4	292.7955	44
GTTACGC	10	8.648788E-4	292.7955	44
ATATCGT	40	0.0	256.19608	44
CAGGTAC	25	1.9474828E-7	234.23639	44
CTATCAT	1240	0.0	200.7066	44
CCGTCTC	95	0.0	200.33376	44
TACCACG	15	0.0029115102	195.197	44
ATATGGG	15	0.0029115102	195.197	44
CCGAAGG	15	0.0029115102	195.197	44
CGGACTT	15	0.0029115102	195.197	44
CATAACC	55	1.8189894E-12	186.32442	44
ACTTCTA	565	0.0	183.96886	44
GTTAGCA	40	9.19681E-9	182.9972	44
GAACACA	855	0.0	171.22543	44
TATTGTT	45	1.8590072E-8	162.66417	44
TTACTTG	55	3.2923708E-10	159.70663	44
GGCAAAT	115	0.0	152.76288	44
>>END_MODULE