##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841017.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21164445
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.8244233193925	39.0	37.0	39.0	32.0	39.0
2	37.65497885722966	39.0	37.0	39.0	35.0	39.0
3	37.80158841868993	39.0	37.0	39.0	35.0	39.0
4	38.09188636886061	39.0	37.0	40.0	35.0	41.0
5	38.76475952948447	40.0	38.0	41.0	35.0	41.0
6	38.77762601381704	40.0	38.0	41.0	35.0	41.0
7	38.81248896439288	40.0	38.0	41.0	35.0	41.0
8	38.77882349383601	40.0	38.0	41.0	35.0	41.0
9	38.80035876206534	40.0	38.0	41.0	35.0	41.0
10	38.79157761991869	40.0	38.0	41.0	35.0	41.0
11	38.716441276867876	40.0	38.0	41.0	35.0	41.0
12	38.65358165546037	40.0	38.0	41.0	35.0	41.0
13	38.59342916858911	40.0	38.0	41.0	35.0	41.0
14	38.58135027873398	40.0	38.0	41.0	35.0	41.0
15	38.58258258130558	40.0	38.0	41.0	35.0	41.0
16	38.514369736603065	40.0	38.0	41.0	35.0	41.0
17	38.529985596125954	40.0	38.0	41.0	35.0	41.0
18	38.55023942276776	40.0	38.0	41.0	35.0	41.0
19	38.53317136357698	40.0	38.0	41.0	35.0	41.0
20	38.484209767844135	40.0	38.0	41.0	35.0	41.0
21	38.40387210720621	40.0	37.0	41.0	34.0	41.0
22	38.31985818668999	40.0	37.0	41.0	34.0	41.0
23	38.25159917966193	40.0	37.0	41.0	34.0	41.0
24	38.123472975549326	40.0	37.0	41.0	34.0	41.0
25	38.00070767154191	39.0	37.0	41.0	34.0	41.0
26	37.877518770505056	39.0	36.0	41.0	33.0	41.0
27	37.77236256079603	39.0	36.0	40.0	33.0	41.0
28	37.65957365710216	39.0	36.0	40.0	33.0	41.0
29	37.61186030724152	39.0	36.0	40.0	33.0	41.0
30	37.54036971696388	39.0	35.0	40.0	33.0	41.0
31	37.46621222120113	38.0	35.0	40.0	33.0	41.0
32	37.400761216374924	38.0	35.0	40.0	33.0	41.0
33	37.301032464698814	38.0	35.0	40.0	33.0	41.0
34	37.168586495910176	38.0	35.0	40.0	33.0	41.0
35	37.07199892180563	38.0	35.0	40.0	32.0	41.0
36	36.996664694018286	38.0	35.0	40.0	32.0	41.0
37	36.88937931093961	38.0	35.0	40.0	32.0	41.0
38	36.754856796451485	38.0	35.0	40.0	32.0	41.0
39	36.653559913581226	38.0	35.0	40.0	31.0	41.0
40	36.448644143363275	37.0	34.0	40.0	31.0	41.0
41	36.28957731716428	37.0	34.0	40.0	31.0	41.0
42	36.14086579782368	37.0	34.0	40.0	31.0	41.0
43	35.97465347985088	36.0	34.0	40.0	31.0	41.0
44	35.76958824322098	36.0	34.0	39.0	30.0	41.0
45	35.53194399229004	36.0	34.0	39.0	29.0	41.0
46	35.108761290927845	36.0	33.0	39.0	28.0	41.0
47	35.04810570070471	36.0	33.0	39.0	29.0	41.0
48	35.82917666409122	36.0	34.0	39.0	31.0	41.0
49	36.55462178560668	37.0	35.0	39.0	32.0	41.0
50	36.07919500665989	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	5.0
20	23.0
21	110.0
22	236.0
23	561.0
24	1282.0
25	2851.0
26	6245.0
27	14213.0
28	31758.0
29	69056.0
30	139183.0
31	267393.0
32	485052.0
33	815227.0
34	1246505.0
35	1465878.0
36	2433585.0
37	3712520.0
38	3701480.0
39	3869390.0
40	2901891.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	56.3474733214124	20.674673963810534	22.977852714777068
2	27.166760101670512	23.397934602112176	32.20249337981696	17.232811916400358
3	26.047803285179462	20.820394770569226	27.273150796063874	25.858651148187445
4	23.123129380430246	25.276434132810948	28.599686880520608	23.000749606238198
5	20.77639172678518	28.8863468897956	27.444452240538318	22.892809142880903
6	22.330115436525737	32.91580761980765	25.572458904544863	19.181618039121744
7	26.088153977106415	31.71625809228638	23.423661711894642	18.77192621871256
8	24.481705048254277	28.09445274846565	27.52672229297768	19.897119910302397
9	22.751491947934376	28.885954722649238	28.34476406066873	20.017789268747656
10	24.722883118361953	26.869332033039374	27.573678402622892	20.834106445975785
11	23.1439284139036	27.05245046586386	29.15769348074093	20.64592763949161
12	23.093182930145346	26.805890728530795	29.183255218835175	20.91767112248868
13	20.80878095315044	27.26866686086028	29.232597405696204	22.689954780293082
14	17.959403140502857	30.322037738291748	29.356451350366147	22.362107770839255
15	18.610840019665055	33.98062174557377	27.594864878337226	19.813673356423948
16	19.724760087023306	37.7687437586953	24.093936788798384	18.41255936548301
17	20.691631649211683	36.58823087494144	23.273901111037876	19.446236364809
18	21.409736943255538	30.928578566553483	25.54106190830896	22.120622581882017
19	20.788440235498733	29.137801629100125	27.697390600131495	22.376367535269647
20	22.236519785895638	27.543004316909798	27.379201297270022	22.841274599924542
21	22.10392004137127	29.28188761859808	27.369439642759353	21.244752697271295
22	21.611537651944097	30.744590751139473	25.999869120121033	21.6440024767954
23	22.715422020279767	30.041038165659433	27.711962208316827	19.531577605743973
24	23.101437339840473	29.81740366921977	26.00643673859626	21.074722252343495
25	21.176435144934842	31.20039663006774	26.23678957375315	21.38637865124427
26	21.596705763080053	32.391744527764075	26.854419924003558	19.157129785152307
27	22.274715802009606	30.113651449338352	25.62298186740589	21.988650881246148
28	21.375415090448104	30.396264747135554	25.798090950440628	22.430229211975718
29	21.9730119508768	31.566044494431612	26.946610871262173	19.51433268342942
30	22.788583208990694	31.373840595108287	26.762814317024365	19.074761878876654
31	21.32247961062134	33.06354596210421	25.471717436344697	20.142256990929752
32	21.274257422929864	32.46056847846946	26.244258697387128	20.020915401213546
33	21.560354756872098	30.426924647450626	27.22946605057073	20.783254545106544
34	20.194991407264713	33.07022576076882	26.02354030381081	20.71124252815566
35	21.540781068194057	31.181289752272036	26.589096461499366	20.688832718034543
36	21.745738929799998	31.04343452387487	25.986091331956807	21.22473521436833
37	20.744892858573678	33.47866655722467	25.87041863519778	19.906021949003875
38	21.865433493996676	32.36091171027127	25.39959038128139	20.37406441445066
39	21.184807932509585	32.93065636587053	26.420735969253162	19.463799732366716
40	20.329594797369634	31.6150176673132	26.665137196856985	21.390250338460184
41	20.84111745301686	33.23227162461324	25.90030895386774	20.026301968502157
42	21.407931961636308	33.58688889570805	25.077198126514936	19.9279810161407
43	20.531558507630898	32.00652075307039	26.15142136137517	21.31049937792354
44	20.759686902842066	31.73837806538745	25.402784264532848	22.099150767237635
45	20.95650394876155	31.502247560550096	27.162190305608032	20.37905818508032
46	21.07988521797561	32.82533155922577	26.594071024739208	19.500712198059407
47	21.354825589737608	33.24395758864811	25.91421255726755	19.487004264346734
48	21.99405417065702	32.400360167980594	25.26180357412549	20.343782087236896
49	20.145719427561133	33.75383515534241	24.4543264928142	21.646118924282256
50	20.23001352715779	34.089841756880126	26.142755366687076	19.537389349275017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129915.0
1	134349.5
2	141342.0
3	150540.0
4	153015.5
5	139211.5
6	122155.0
7	112563.0
8	107606.5
9	104476.0
10	104297.5
11	104601.5
12	104324.5
13	103759.0
14	107178.0
15	115283.0
16	125388.0
17	140426.0
18	163893.5
19	194695.5
20	220747.5
21	249547.0
22	284831.5
23	328085.5
24	368525.0
25	399887.5
26	444038.0
27	499116.0
28	553594.0
29	609178.0
30	670512.5
31	741772.5
32	845722.0
33	938540.5
34	1016684.5
35	1095133.0
36	1149635.5
37	1209844.0
38	1269619.0
39	1325515.0
40	1386368.0
41	1454787.0
42	1497165.0
43	1507846.0
44	1520247.0
45	1525626.5
46	1525570.5
47	1496732.0
48	1493845.0
49	1525474.5
50	1606729.5
51	1654041.5
52	1543322.0
53	1365191.0
54	1190189.5
55	1065456.5
56	969950.0
57	867311.0
58	746396.0
59	623749.5
60	543001.5
61	478169.5
62	413263.5
63	339924.0
64	256898.0
65	196888.5
66	146403.0
67	101698.0
68	70257.0
69	49537.0
70	37220.0
71	28427.5
72	21595.0
73	14909.0
74	9380.5
75	6158.5
76	4606.5
77	3604.0
78	2860.0
79	2314.0
80	1678.5
81	957.5
82	490.0
83	249.0
84	120.5
85	81.5
86	61.5
87	55.0
88	45.5
89	36.0
90	38.0
91	35.5
92	28.5
93	27.0
94	30.0
95	33.0
96	33.0
97	33.5
98	18.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	86870.0
25	91592.0
26	82686.0
27	98494.0
28	84817.0
29	79440.0
30	135262.0
31	88750.0
32	92643.0
33	91102.0
34	95418.0
35	96631.0
36	97147.0
37	97332.0
38	96849.0
39	130413.0
40	152015.0
41	142357.0
42	183893.0
43	343885.0
44	824252.0
45	1834530.0
46	2575849.0
47	2246387.0
48	3728785.0
49	2957840.0
50	4629206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.803200843715263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.77953408972104	8.236218556418534
2	12.541974225559729	2.960708815219634
3	4.206827693034291	1.4896209652736123
4	1.9126078622975002	0.9029957893586521
5	1.0917555597354762	0.6443105071900301
6	0.7067964973591355	0.5005476608378605
7	0.5481195166442985	0.45286953189189505
8	0.422116433040363	0.3985860030886473
9	0.34491445365660856	0.3663985113368345
>10	4.323404621290409	12.12863533623762
>50	2.092317673598317	18.259679337117
>100	1.93562908544259	36.89741438504186
>500	0.06088542074429696	4.889743632174439
>1k	0.02994399433833102	6.4899244196447174
>5k	0.002115249025061686	1.693078365592537
>10k+	0.001057624512530843	3.6892681835763783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	112038	0.5293689487250907	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	73186	0.34579692498433107	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	45788	0.21634396744162204	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	43020	0.20326542935569536	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	32382	0.15300188594598157	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	27236	0.1286875228714951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02536801697375008	0.0	0.0	0.0	0.0
2	0.049328012144896786	0.0	0.0	0.0	0.0
3	0.06841190496608818	0.0	0.0	0.0	0.0
4	0.08742964911198947	0.0	0.0	0.0	0.0
5	0.10542681369627221	0.0	0.0	0.0	0.0
6	0.13851060115207367	0.0	0.0	0.0	0.0
7	0.16576385537159136	0.0	0.0	0.0	0.0
8	0.19238397227047532	0.0	0.0	0.0	0.0
9	0.21692040589772138	0.0	0.0	0.0	0.0
10	0.2402803380858794	0.0	0.0	0.0	0.0
11	0.26208577640472025	0.0	0.0	0.0	0.0
12	0.2854315338767447	0.0	0.0	0.0	0.0
13	0.30752519142363527	0.0	0.0	0.0	0.0
14	0.33151353602704914	0.0	0.0	0.0	0.0
15	0.3571083484589367	9.827803185956448E-4	0.0	0.0	0.0
16	0.38337409745448087	9.827803185956448E-4	0.0	0.0	0.0
17	0.4094225008026433	9.827803185956448E-4	0.0	0.0	0.0
18	0.43582054714876767	9.827803185956448E-4	0.0	0.0	0.0
19	0.46341399455549154	9.827803185956448E-4	0.0	0.0	0.0
20	0.4905444012351848	9.827803185956448E-4	0.0	0.0	0.0
21	0.5173865886868283	9.827803185956448E-4	0.0	0.0	0.0
22	0.5435436648586817	9.827803185956448E-4	0.0	0.0	0.0
23	0.5708630677534894	9.827803185956448E-4	0.0	0.0	0.0
24	0.5964767798068884	9.827803185956448E-4	0.0	0.0	0.0
25	0.6244860188868643	9.827803185956448E-4	0.0	0.0	0.0
26	0.652396034953905	9.827803185956448E-4	0.0	0.0	0.0
27	0.6780900703987277	9.827803185956448E-4	0.0	0.0	0.0
28	0.7081310187911849	9.827803185956448E-4	0.0	0.0	0.0
29	0.7382522905750659	9.827803185956448E-4	0.0	0.0	0.0
30	0.7687326551676644	9.827803185956448E-4	0.0	0.0	0.0
31	0.7991326963688393	9.827803185956448E-4	0.0	0.0	0.0
32	0.8297028341636173	9.92230129351372E-4	0.0	0.0	0.0
33	0.8594650131387807	9.92230129351372E-4	0.0	0.0	0.0
34	0.8903091954454747	9.92230129351372E-4	0.0	0.0	0.0
35	0.9182381111340269	9.92230129351372E-4	0.0	0.0	0.0
36	0.9414657459716047	9.92230129351372E-4	0.0	0.0	0.0
37	0.9632286601420448	9.92230129351372E-4	0.0	0.0	0.0
38	0.9774175509917694	9.92230129351372E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGC	18260	0.0	75.92856	43
CTATCAT	7805	0.0	64.882126	44
TCGTGCG	4175	0.0	56.290184	43
ACCTTAG	18565	0.0	52.625084	42
CCCTATC	7100	0.0	49.20109	42
AACCTTA	26740	0.0	48.60811	41
CAACCTT	28215	0.0	40.431744	40
CGTGCGC	2935	0.0	40.227913	44
CCGCTCT	8520	0.0	39.733067	44
ACAACCT	32005	0.0	38.953148	39
GACAACC	32180	0.0	38.79521	38
CCTATCA	8165	0.0	38.629543	43
GACGCTA	7360	0.0	38.17213	31
CACCTTA	31835	0.0	37.80376	25
ACCTTAC	32485	0.0	37.25756	26
CTCGTGC	5030	0.0	37.239605	42
CCGCGGC	12120	0.0	37.204964	2
CGCGGCC	12305	0.0	36.72766	3
CTACCAG	33525	0.0	36.501602	32
AGACAAC	34190	0.0	36.297817	37
>>END_MODULE