FastQCFastQC Report
Tue 24 May 2016
ERR841016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033464
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC151841.4692335678843191No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA100830.9756508209284503No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT81200.7857070976831316No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT57740.5587035445840397No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA50970.4931956991244978No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC47360.458264632343265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA44230.4279781395384842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA42190.4082387001385631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA39700.38414497263571834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA34560.33440932630454473No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA33360.3227978913634147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32090.31050912271738546No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA31330.3031552139213364No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA30570.29580130512528735No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25260.24442070551078704No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG24720.2391955597872785No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA24510.23716355867258077No Hit
AAAAAAAAAAAAAAAAAAAAAAAA24430.23638946300983876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24310.23522831951572576No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC21810.21103783005503818No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG21690.2098766865609252No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG19990.19342715372765767No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA18450.17852581221987412No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC18280.17688085893654737No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC17690.1711719034238251No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAA15380.1488198911621498No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA15290.14794903354156508No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG15140.14649760417392382No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG15000.14514293676412532No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC14790.14311093564942756No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC14520.1404983627876733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14220.1375955040523908No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13230.1280160702259585No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG13030.1260808310691035No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC12930.12511321149067603No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.12501644953283325No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA12610.122016828839708No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.12143625709265152No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12530.121242733176966No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA12510.12104920926128052No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC12440.12037187555638126No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT11490.11117948956131998No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.10982482215152148No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11110.10750253516329548No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG11100.10740577320545272No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC11040.10682520145839623No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG10920.10566405796428321No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA10750.10401910468095647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAT151.3376699E-5252.8437744
CATAAGT151.3376699E-5252.8437744
ATGCGCC151.3376699E-5252.8437744
ACCGTGA201.3310637E-7252.8437544
TCCTACG451.8189894E-12196.6562744
CTAGCTT204.214365E-5189.6328144
GGGTAGT150.004516543168.562544
TTCGTGA504.7293724E-10151.7062544
GCGTAAC251.0256534E-4151.7062544
CTATCAT13750.0150.7868244
TAATTCC1450.0148.2187544
ACATTGG3200.0146.175344
CCCATAC4400.0129.295144
AACGTTC302.1200896E-4126.4218844
AGTTAGC204.391057E-6125.3578643
GGTTATA204.391057E-6125.3578643
GTTAGAT301.7880666E-9125.3578543
TATGCGC152.1946999E-4125.3578543
TATAGCC258.833922E-8125.3578543
GCGTAGA152.1946999E-4125.3578543
Produced by FastQC (version 0.11.5)