##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841016.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033464
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.943514239489716	39.0	37.0	39.0	33.0	39.0
2	37.60779185341725	39.0	37.0	39.0	35.0	39.0
3	37.929999496837816	39.0	38.0	39.0	35.0	39.0
4	38.5773960196001	39.0	38.0	41.0	35.0	41.0
5	39.19527917760077	40.0	38.0	41.0	35.0	41.0
6	39.17179117995402	40.0	38.0	41.0	35.0	41.0
7	39.16886896882717	40.0	38.0	41.0	35.0	41.0
8	39.158559949838605	40.0	38.0	41.0	35.0	41.0
9	39.15181660899654	40.0	38.0	41.0	35.0	41.0
10	39.037107243213114	40.0	38.0	41.0	35.0	41.0
11	38.99836375529288	40.0	38.0	41.0	35.0	41.0
12	38.925584248701455	40.0	38.0	41.0	35.0	41.0
13	38.792519139515264	40.0	38.0	41.0	35.0	41.0
14	38.731376225974	40.0	38.0	41.0	35.0	41.0
15	38.68984115557001	40.0	38.0	41.0	35.0	41.0
16	38.620159966868705	40.0	38.0	41.0	35.0	41.0
17	38.5286396042823	40.0	37.0	41.0	35.0	41.0
18	38.48553215206335	40.0	37.0	41.0	35.0	41.0
19	38.43329327388279	40.0	37.0	41.0	35.0	41.0
20	38.358197286020605	40.0	37.0	41.0	35.0	41.0
21	38.246527213333025	40.0	37.0	41.0	34.0	41.0
22	38.20202638892114	40.0	36.0	41.0	34.0	41.0
23	38.0425578442984	40.0	36.0	41.0	34.0	41.0
24	37.78123572761122	39.0	36.0	41.0	33.0	41.0
25	37.73223295288265	39.0	36.0	41.0	33.0	41.0
26	37.60582818007499	39.0	35.0	41.0	33.0	41.0
27	37.456227971488616	39.0	35.0	41.0	33.0	41.0
28	37.35219379719743	39.0	35.0	41.0	33.0	41.0
29	37.33231608131196	38.0	35.0	41.0	33.0	41.0
30	37.26413240918525	38.0	35.0	40.0	33.0	41.0
31	37.232398375646014	38.0	35.0	40.0	33.0	41.0
32	37.16032279387463	38.0	35.0	40.0	32.0	41.0
33	37.02871139614984	38.0	35.0	40.0	32.0	41.0
34	36.965502818890336	38.0	35.0	40.0	32.0	41.0
35	36.825413280391714	38.0	35.0	40.0	32.0	41.0
36	36.78251347321299	38.0	35.0	40.0	32.0	41.0
37	36.70900421753803	38.0	35.0	40.0	32.0	41.0
38	36.48394769057813	37.0	34.0	40.0	31.0	41.0
39	36.39430836057498	37.0	34.0	40.0	31.0	41.0
40	36.18842750923908	37.0	34.0	40.0	31.0	41.0
41	36.06409709393106	37.0	34.0	40.0	31.0	41.0
42	35.89530920567952	36.0	34.0	40.0	30.0	41.0
43	35.793274931380445	36.0	34.0	40.0	30.0	41.0
44	35.57075612240975	36.0	34.0	40.0	29.0	41.0
45	35.310597362613755	36.0	33.0	40.0	29.0	41.0
46	34.98327476001343	35.0	33.0	39.0	28.0	41.0
47	35.13667102706063	35.0	33.0	39.0	29.0	41.0
48	36.03348378629278	37.0	34.0	40.0	31.0	41.0
49	36.5655212343129	37.0	35.0	40.0	32.0	41.0
50	36.53034789752211	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	35.0
22	45.0
23	109.0
24	212.0
25	392.0
26	612.0
27	1093.0
28	2232.0
29	4131.0
30	7838.0
31	14198.0
32	26026.0
33	46152.0
34	69798.0
35	64556.0
36	114357.0
37	185406.0
38	166889.0
39	142427.0
40	186948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.99182167932313	20.896325367888963	36.111852952787906
2	22.039084090011844	23.97258153162568	32.267693891611124	21.720640486751353
3	21.67506560460742	27.466268781496016	27.907793595132485	22.95087201876408
4	20.105393124482323	28.31603229527105	29.30339131309847	22.275183267148154
5	17.832938544545335	28.832741150151335	29.022297825565285	24.312022479738047
6	20.010663167754274	29.814971784213096	23.416200274029862	26.758164774002775
7	24.13127114248779	27.016712725358598	21.93593584295147	26.91608028920214
8	24.08724445166934	26.688689688271676	26.13327605025429	23.090789809804697
9	24.01341507783532	29.771719189057382	25.615019004048523	20.599846729058775
10	26.388243809169936	23.37797930068198	26.28103155988017	23.952745330267913
11	22.948549731775852	25.81589682853007	25.293672542052747	25.94188089764133
12	22.763927916211884	27.443142673571604	25.953008522793247	23.83992088742327
13	24.8064760843145	26.693237500290284	23.62501257905452	24.875273836340696
14	19.92793169379872	29.559616977466074	22.3746545598105	28.137796768924705
15	21.381586586470355	32.4201907371713	23.786992096483285	22.41123057987506
16	20.849299056377387	33.720381164704335	20.076267775171655	25.354052003746624
17	21.059659552727524	36.303538391274394	21.05104773847952	21.58575431751856
18	19.935285602594767	30.9333464929596	21.02908277404922	28.102285130396414
19	22.401844669964312	27.605025429042524	21.381876872343884	28.61125302864928
20	22.469578040454238	26.44707507953833	25.021287630725404	26.062059249282026
21	19.218182733022147	36.30111934232833	22.24199391560809	22.238704009041438
22	24.551895373230224	29.6934387651626	20.604007493246016	25.15065836836116
23	24.972713127888344	27.957335717547977	25.44152481363647	21.628426340927213
24	23.195873296021922	28.892443278140313	24.730711471323627	23.180971954514142
25	21.776607160277045	29.81455467759243	22.824602477807044	25.58423568432348
26	23.980649551841335	36.03597481526566	21.673329216635793	18.310046416257215
27	22.303889573694434	32.07500729924098	21.982822618977625	23.63828050808696
28	23.39225861163833	28.95822396317086	20.97807212373299	26.67144530145782
29	21.364850072124973	33.32900242462684	23.579444893449416	21.726702609798767
30	25.535479846518744	31.349261037293708	22.90717036812912	20.20808874805843
31	24.18475611098704	33.08504581470915	21.49469505616352	21.235503018140296
32	22.582012016847315	29.77612477860357	23.521940492029728	24.119922712519383
33	20.43373741568536	27.664085762786605	29.08279799378785	22.819378827740184
34	22.328521995968394	32.4205805943924	23.070919615811658	22.179977793827547
35	20.147045559999956	27.098132156273397	23.696481099342684	29.058341184383963
36	19.962815137328715	28.359319278171043	25.25853559130396	26.41932999319629
37	20.55716071324161	34.857626374450746	22.72969475527137	21.855518157036272
38	25.77945348829459	27.63829981048277	20.88944235721221	25.692804344010433
39	21.285768662397565	31.53318257131342	24.279812816264386	22.901235950024628
40	19.91551227602788	27.935519077526923	26.142047386789503	26.0069212596557
41	20.39350038034557	32.41367871380283	22.960404024227728	24.232416881623877
42	18.533142273587842	33.70307788922929	22.98615279459574	24.777627042587124
43	21.10793873664883	25.19341440106692	23.970002277535286	29.728644584748963
44	22.342230210295643	27.035316789050786	21.03208738610588	29.590365614547693
45	22.066054839427313	25.11986375805804	29.371159565191817	23.442921837322825
46	19.762705682094513	29.719108604777727	26.68309035537697	23.835095357750784
47	21.567555936568223	32.68416972065016	24.06254376048117	21.685730582300444
48	23.790347535665887	29.132814750792278	22.05149545973516	25.02534225380667
49	19.686727380840612	30.285732169123403	21.378900259756517	28.648640190279473
50	21.475014169357255	31.519655605015632	25.252023511580663	21.75330671404645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53521.0
1	49361.5
2	35701.5
3	24997.5
4	21798.5
5	14572.5
6	6412.5
7	3504.0
8	3341.0
9	3045.0
10	2713.5
11	2431.0
12	2402.5
13	2675.0
14	3591.5
15	4738.5
16	5044.5
17	4365.0
18	3849.5
19	4028.5
20	4283.5
21	4506.5
22	4878.5
23	5810.5
24	6899.5
25	7899.5
26	8640.5
27	8879.5
28	9471.0
29	10750.0
30	13167.5
31	19134.5
32	27959.5
33	30828.0
34	30904.0
35	34027.5
36	34244.0
37	32339.0
38	31751.0
39	32742.0
40	34653.5
41	37671.5
42	42022.5
43	44769.5
44	46252.0
45	49828.5
46	55752.0
47	59038.5
48	65766.0
49	78110.5
50	94192.0
51	106986.5
52	103095.0
53	92061.0
54	82819.0
55	77662.0
56	73689.5
57	69712.0
58	71131.5
59	71308.5
60	64034.5
61	56692.0
62	51662.0
63	41784.5
64	29931.0
65	22861.5
66	17581.0
67	12822.5
68	9789.5
69	7501.5
70	5465.5
71	3855.5
72	2866.0
73	2241.0
74	1713.5
75	1245.0
76	878.0
77	458.5
78	290.0
79	275.0
80	221.0
81	178.5
82	114.5
83	58.5
84	39.5
85	27.5
86	18.0
87	10.0
88	7.0
89	6.5
90	4.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	8364.0
25	9723.0
26	11848.0
27	13009.0
28	13050.0
29	12401.0
30	11336.0
31	9725.0
32	9710.0
33	6628.0
34	7411.0
35	7522.0
36	6284.0
37	6271.0
38	6942.0
39	8141.0
40	10894.0
41	9554.0
42	12853.0
43	19446.0
44	50214.0
45	91266.0
46	105298.0
47	116989.0
48	149055.0
49	162501.0
50	157029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.393470859866516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5808075928126	9.319285189003143
2	11.117545479005745	2.9780504281260836
3	4.289563105584058	1.723564153685958
4	2.281901844102462	1.2225034341624796
5	1.4117840862370132	0.9454344509719359
6	1.0278393087994024	0.8259801498618083
7	0.8219500831934968	0.7706135141261871
8	0.5745480277331318	0.6156153813629984
9	0.506755347044197	0.6108491676346196
>10	5.004709083215146	15.423849654849414
>50	1.957679319669957	18.714250045694
>100	1.3430677202600807	29.47067844311812
>500	0.04613307404771468	4.264681241241977
>1k	0.03199551909760857	8.703963971855018
>5k	0.0022322455184378073	1.8926123770809804
>10k+	0.001488163678958538	2.5180683972252713
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	15184	1.4692335678843191	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10083	0.9756508209284503	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8120	0.7857070976831316	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5774	0.5587035445840397	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	5097	0.4931956991244978	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4736	0.458264632343265	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4423	0.4279781395384842	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	4219	0.4082387001385631	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	3970	0.38414497263571834	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3456	0.33440932630454473	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3336	0.3227978913634147	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3209	0.31050912271738546	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	3133	0.3031552139213364	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3057	0.29580130512528735	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2526	0.24442070551078704	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	2472	0.2391955597872785	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2451	0.23716355867258077	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2443	0.23638946300983876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2431	0.23522831951572576	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2181	0.21103783005503818	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	2169	0.2098766865609252	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG	1999	0.19342715372765767	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1845	0.17852581221987412	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1828	0.17688085893654737	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1769	0.1711719034238251	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAA	1538	0.1488198911621498	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1529	0.14794903354156508	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	1514	0.14649760417392382	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1500	0.14514293676412532	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	1479	0.14311093564942756	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1452	0.1404983627876733	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1422	0.1375955040523908	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1323	0.1280160702259585	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1303	0.1260808310691035	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	1293	0.12511321149067603	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1292	0.12501644953283325	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	1261	0.122016828839708	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1255	0.12143625709265152	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.121242733176966	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1251	0.12104920926128052	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC	1244	0.12037187555638126	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1149	0.11117948956131998	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1135	0.10982482215152148	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.10750253516329548	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	1110	0.10740577320545272	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1104	0.10682520145839623	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	1092	0.10566405796428321	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	1075	0.10401910468095647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10479320034369848	0.0	0.0	0.0	0.0
2	0.21171516375993746	0.0	0.0	0.0	0.0
3	0.2863186332566979	0.0	0.0	0.0	0.0
4	0.3430211405525495	0.0	0.0	0.0	0.0
5	0.3961434554082193	0.0	0.0	0.0	0.0
6	0.467553780296169	0.0	0.0	0.0	0.0
7	0.5214501908145809	0.0	0.0	0.0	0.0
8	0.5749595535016218	0.0	0.0	0.0	0.0
9	0.6236308182965251	0.0	0.0	0.0	0.0
10	0.6712377015551582	0.0	0.0	0.0	0.0
11	0.7289078284294374	0.0	0.0	0.0	0.0
12	0.7886099564184141	0.0	0.0	0.0	0.0
13	0.8507311333534598	0.0	0.0	0.0	0.0
14	0.9053048775767709	0.0	0.0	0.0	0.0
15	0.9652972914392761	0.0	0.0	0.0	0.0
16	1.0128074127400664	0.0	0.0	0.0	0.0
17	1.0756059233800113	0.0	0.0	0.0	0.0
18	1.1172135652523938	0.0	0.0	0.0	0.0
19	1.1661751159208256	0.0	0.0	0.0	0.0
20	1.203138183816756	0.0	0.0	0.0	0.0
21	1.2405850615019005	0.0	0.0	0.0	0.0
22	1.2691298390655117	0.0	0.0	0.0	0.0
23	1.303867381931059	0.0	0.0	0.0	0.0
24	1.3369599715132796	0.0	0.0	0.0	0.0
25	1.369665513264129	0.0	0.0	0.0	0.0
26	1.4156274432394356	0.0	0.0	0.0	0.0
27	1.4481394610745997	0.0	0.0	0.0	0.0
28	1.4826834800244615	0.0	0.0	0.0	0.0
29	1.5224526446978317	0.0	0.0	0.0	0.0
30	1.5626088572025731	0.0	0.0	0.0	0.0
31	1.6016039262132014	0.0	0.0	0.0	0.0
32	1.638566994109132	0.0	0.0	0.0	0.0
33	1.6728207271854656	0.0	0.0	0.0	0.0
34	1.705235983062787	0.0	0.0	0.0	0.0
35	1.7339742845420838	0.0	0.0	0.0	0.0
36	1.7589388696655133	0.0	0.0	0.0	0.0
37	1.7778074514448496	0.0	0.0	0.0	0.0
38	1.7914508875006774	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTAAT	15	1.3376699E-5	252.84377	44
CATAAGT	15	1.3376699E-5	252.84377	44
ATGCGCC	15	1.3376699E-5	252.84377	44
ACCGTGA	20	1.3310637E-7	252.84375	44
TCCTACG	45	1.8189894E-12	196.65627	44
CTAGCTT	20	4.214365E-5	189.63281	44
GGGTAGT	15	0.004516543	168.5625	44
TTCGTGA	50	4.7293724E-10	151.70625	44
GCGTAAC	25	1.0256534E-4	151.70625	44
CTATCAT	1375	0.0	150.78682	44
TAATTCC	145	0.0	148.21875	44
ACATTGG	320	0.0	146.1753	44
CCCATAC	440	0.0	129.2951	44
AACGTTC	30	2.1200896E-4	126.42188	44
AGTTAGC	20	4.391057E-6	125.35786	43
GGTTATA	20	4.391057E-6	125.35786	43
GTTAGAT	30	1.7880666E-9	125.35785	43
TATGCGC	15	2.1946999E-4	125.35785	43
TATAGCC	25	8.833922E-8	125.35785	43
GCGTAGA	15	2.1946999E-4	125.35785	43
>>END_MODULE