FastQCFastQC Report
Tue 24 May 2016
ERR841015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548327
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC131602.40002772068492No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA84071.5332091981609512No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT52450.9565460026589973No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA42790.7803737550768064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA41010.7479113740523448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA38020.6933818688483332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA34530.6297337172891359No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT33540.6116787975058678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA29980.5467540354569445No Hit
AAAAAAAAAAAAAAAAAAAAAAAA22780.41544552794226797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22640.412892306962816No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC19370.35325635979990044No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC16170.2948970231267109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12540.2286956505880615No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA12350.22523056497309088No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10910.1989688634701556No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA10510.19167394638600688No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA10210.18620275857289537No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA8390.15301088584001882No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA8280.15100478364187794No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8270.1508224107147742No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT7590.13842105167172145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.13203799922309134No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG6840.12474308213894264No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA6540.11927189432583113No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC6200.11307121480430474No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT6200.11307121480430474No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT5980.10905901040802295No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.10577629772015604No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.10504680601174116No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA5530.10085222868835567No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG5520.10066985576125197Illumina Single End Adapter 1 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5510.10048748283414824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGC100.002568069203.536544
CTAGATA100.002568069203.536544
GACACGA100.002568069203.536544
GACGACG203.9186125E-7203.536544
TACGGAT153.1750795E-5203.5364844
CCCGTTC153.1750795E-5203.5364844
AGTAGGC153.1750795E-5203.5364844
CAGACTA306.002665E-11203.5364844
CTCCGCG351.7826096E-10174.4598544
GAATATT301.4455509E-8169.6137444
TACGGAC301.4455509E-8169.6137444
ATCGAAA550.0166.5298644
GGACACT251.190996E-6162.829244
TGGTAAC251.190996E-6162.829244
CTGTACG404.5110937E-10152.6523744
CTATCAT4000.0147.5639544
CACGCTA150.008635419135.69144
GCTCGGC302.9515068E-6135.69144
CCGCATC150.008635419135.69144
GCATCAT302.9515068E-6135.69144
Produced by FastQC (version 0.11.5)