##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841015.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 548327 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.276570367682055 39.0 37.0 39.0 30.0 39.0 2 37.229572864367434 39.0 37.0 39.0 33.0 39.0 3 37.55910980126822 39.0 37.0 39.0 34.0 39.0 4 38.044528173881645 39.0 37.0 40.0 35.0 41.0 5 38.63460672190135 40.0 38.0 41.0 35.0 41.0 6 38.60614742662681 40.0 38.0 41.0 35.0 41.0 7 38.60433646346067 40.0 38.0 41.0 35.0 41.0 8 38.47561035659378 40.0 37.0 41.0 35.0 41.0 9 38.43398373598236 40.0 37.0 41.0 35.0 41.0 10 38.35619621138481 40.0 37.0 41.0 35.0 41.0 11 38.230707223974015 40.0 37.0 41.0 34.0 41.0 12 38.116715025887835 39.0 36.0 41.0 34.0 41.0 13 37.96620082541987 39.0 36.0 41.0 34.0 41.0 14 37.85082076935843 39.0 35.0 40.0 34.0 41.0 15 37.71294683646802 39.0 35.0 40.0 33.0 41.0 16 37.67154818201548 39.0 35.0 40.0 33.0 41.0 17 37.60047015740607 39.0 35.0 40.0 33.0 41.0 18 37.5401831388934 39.0 35.0 40.0 33.0 41.0 19 37.45966002038929 39.0 35.0 40.0 33.0 41.0 20 37.39507082452624 38.0 35.0 40.0 33.0 41.0 21 37.331367961088915 38.0 35.0 40.0 33.0 41.0 22 37.19247821099454 38.0 35.0 40.0 33.0 41.0 23 37.02572187763871 38.0 35.0 40.0 33.0 41.0 24 36.68601947378116 38.0 35.0 40.0 33.0 41.0 25 36.5166268988077 38.0 35.0 40.0 32.0 41.0 26 36.3075506617812 37.0 35.0 40.0 31.0 41.0 27 36.18745869949946 37.0 35.0 40.0 31.0 41.0 28 36.01727267098532 37.0 34.0 40.0 31.0 41.0 29 35.904939613359815 37.0 34.0 39.0 31.0 41.0 30 35.88332165452271 36.0 34.0 39.0 31.0 41.0 31 35.838615586877424 36.0 34.0 39.0 31.0 41.0 32 35.784445311161875 36.0 34.0 39.0 31.0 41.0 33 35.692992830756204 36.0 34.0 39.0 31.0 41.0 34 35.62774963036807 36.0 34.0 39.0 31.0 41.0 35 35.35851544238008 35.0 34.0 38.0 31.0 40.0 36 35.27148205994993 35.0 34.0 38.0 31.0 40.0 37 35.20933630435439 35.0 34.0 38.0 31.0 40.0 38 35.02587389514995 35.0 34.0 38.0 30.0 40.0 39 34.91416770706472 35.0 33.0 37.0 30.0 40.0 40 34.62914027149321 35.0 33.0 37.0 30.0 40.0 41 34.5046110217935 35.0 33.0 37.0 30.0 40.0 42 34.4658612343596 35.0 33.0 37.0 30.0 39.0 43 34.26413551081583 35.0 33.0 37.0 29.0 39.0 44 34.029215627712496 35.0 32.0 36.0 29.0 39.0 45 33.89527413643217 35.0 32.0 36.0 29.0 39.0 46 33.52264344431764 35.0 32.0 36.0 27.0 39.0 47 33.444015166372495 35.0 32.0 36.0 28.0 38.0 48 34.19530507209366 35.0 33.0 36.0 30.0 39.0 49 34.79166857771866 35.0 34.0 36.0 31.0 39.0 50 34.63587965464065 35.0 34.0 37.0 30.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 5.0 21 20.0 22 60.0 23 104.0 24 207.0 25 377.0 26 612.0 27 1094.0 28 2035.0 29 3855.0 30 7100.0 31 12650.0 32 22424.0 33 38460.0 34 56019.0 35 65060.0 36 88265.0 37 90865.0 38 77082.0 39 56153.0 40 25877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 47.762010625046734 16.76098386546714 35.477005509486126 2 21.381401973639814 28.54099834587755 32.85849502213096 17.21910465835168 3 19.82247819275724 30.71269516182862 23.746414092320823 25.718412553093316 4 17.515643037822322 33.440082286664705 27.573692340519436 21.470582334993534 5 16.219883390750407 35.421928885500805 27.070343061713174 21.28784466203561 6 18.455775477041982 34.08495295690345 23.229751589835992 24.22951997621857 7 21.22802634194559 31.254342755326658 21.533136248990107 25.984494653737645 8 21.398362655860463 31.216956305270394 27.691505251428435 19.693175787440705 9 20.245036264856555 35.60594316894846 25.062234761374143 19.086785804820845 10 25.541146067948507 29.552073853740563 23.93134024040399 20.975439837906944 11 20.727230284118782 29.204106308826667 24.44289630092992 25.62576710612463 12 20.871669642384926 30.120347894595746 23.625318468723954 25.382663994295374 13 19.818283615433856 30.627344631944077 22.238372358100182 27.315999394521885 14 17.54208711225236 30.58776970676257 24.16185962026309 27.70828356072198 15 18.1229448850777 40.767461751837864 23.5195421710038 17.59005119208064 16 19.26459940874697 41.64431078535253 19.288855008051765 19.802234797848726 17 18.79371251096517 42.61562899510694 19.921324319247457 18.669334174680436 18 18.97079662318288 31.368143461839377 22.664577888741572 26.99648202623617 19 18.24914695063347 29.47182976581492 24.61323261484479 27.665790668706812 20 18.20282422714913 29.332679222434788 24.35608678762855 28.108409762787534 21 19.946309410260664 36.98559436248808 22.072960113217114 20.995136114034143 22 21.738852910763104 30.276094374342318 21.485901660870248 26.499151054024335 23 23.663799156342836 30.204421813990557 25.835495972293902 20.296283057372698 24 20.548322442630038 32.27599589296168 23.240146846680904 23.935534817727376 25 19.509730525120133 30.82660598841262 22.47867471612387 27.18498877034338 26 19.933634936065207 38.812158827488226 23.096350856202797 18.157855380243777 27 22.095672846867238 30.454894156786196 23.786429889789375 23.66300310655719 28 20.8003436346328 30.01946488967316 20.865355592189278 28.314835883504763 29 20.323561145048256 30.72390987469008 28.860928275701554 20.091600704560115 30 21.930396965784766 30.82643815015671 28.58410651075287 18.65905837330565 31 22.883582515465598 33.48090622802458 24.493023919446397 19.14248733706342 32 20.273139807712546 28.59238898278328 24.497768202613774 26.636703006890407 33 20.303331810625167 26.356201072570602 32.41303354755543 20.927433569248805 34 21.787987961461532 34.65959160667377 23.934061450387297 19.618358981477392 35 20.18432768800596 27.037912226730278 23.535006762078854 29.242753323184907 36 19.064378784312886 29.326686493078586 26.508269325402768 25.100665397205756 37 20.492071769984356 38.08071752744387 20.83988811261147 20.587322589960305 38 26.66313577717193 28.754753677045166 22.461883758487158 22.120226787295742 39 19.230929011691085 35.34394357143326 25.19519762461272 20.229929792262936 40 18.989294779825627 28.279218629290366 25.904867012471026 26.82661957841298 41 17.733118971061092 36.84820471596999 22.677518756698824 22.741157556270096 42 18.73509833687853 34.45907385811248 24.626513851074723 22.179313953934265 43 19.24412600641547 26.3223910877969 23.62163347742248 30.81184942836515 44 20.6749391000812 26.893497475336698 23.564301914264114 28.867261510317988 45 19.999950884810143 26.6268178759645 32.43125101300079 20.941980226224565 46 18.71185431449412 31.388489168968505 28.585763354780376 21.313893161757 47 19.79898019219455 34.968948159769894 25.355625286003793 19.87644636203177 48 25.587553530035752 26.729658586414175 23.49664086748124 24.186147016068833 49 17.854767692519378 28.59812869788561 23.442301518139704 30.1048020914553 50 17.432003693262743 34.31591147956428 27.320321785338926 20.93176304183406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40536.0 1 38231.0 2 24942.5 3 13720.0 4 12926.5 5 9292.5 6 4366.5 7 2571.5 8 2710.5 9 2794.5 10 2704.0 11 2620.5 12 2451.0 13 2072.0 14 1751.0 15 1663.0 16 1772.5 17 1940.5 18 2054.5 19 2211.5 20 2454.5 21 2622.0 22 2783.5 23 3482.5 24 4314.0 25 5289.5 26 6257.5 27 6716.5 28 7278.0 29 8674.0 30 10503.5 31 12595.0 32 15961.0 33 18491.5 34 19043.0 35 19201.5 36 19491.5 37 20433.5 38 22054.0 39 24264.0 40 25573.5 41 25641.5 42 26104.5 43 27149.0 44 27878.0 45 28334.5 46 30023.0 47 32066.5 48 37820.5 49 47250.0 50 58867.0 51 63893.0 52 53907.5 53 41512.5 54 35557.5 55 33617.0 56 33009.0 57 31427.5 58 28585.5 59 25537.0 60 22004.0 61 18662.5 62 16042.5 63 12864.0 64 9150.5 65 6856.0 66 5388.0 67 4246.5 68 3593.5 69 2837.0 70 1927.0 71 1333.0 72 997.0 73 797.5 74 711.0 75 565.0 76 443.5 77 267.5 78 122.0 79 82.5 80 52.5 81 30.0 82 22.0 83 15.5 84 10.5 85 8.0 86 5.0 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 6013.0 25 7394.0 26 8292.0 27 9800.0 28 9845.0 29 8930.0 30 7448.0 31 6018.0 32 5178.0 33 3936.0 34 4464.0 35 3619.0 36 3352.0 37 3342.0 38 3339.0 39 4307.0 40 5210.0 41 4517.0 42 5631.0 43 9120.0 44 21366.0 45 44452.0 46 56814.0 47 51410.0 48 80106.0 49 72617.0 50 101807.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.41115794042606 #Duplication Level Percentage of deduplicated Percentage of total 1 66.8275833933938 8.96235275665798 2 11.282755619620055 3.0262963523590853 3 4.505215061805622 1.8126045224838465 4 2.393353005969784 1.2839054068101698 5 1.5719977698301535 1.0541155186594862 6 1.1178046425608876 0.8994632764755338 7 0.9083862545385316 0.8527758071369821 8 0.7479228143655574 0.8024408792563562 9 0.6010579708174116 0.7254795040185874 >10 6.562682731142146 21.124803265204886 >50 2.3036022682459167 21.852835990202927 >100 1.1218842215483362 23.610546261628553 >500 0.03127677223710513 2.5855009875494 >1k 0.020397894937242474 6.517096550051338 >5k 0.0027197193249656635 2.489755200819949 >10k+ 0.0013598596624828318 2.40002772068492 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 13160 2.40002772068492 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 8407 1.5332091981609512 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 5245 0.9565460026589973 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 4279 0.7803737550768064 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 4101 0.7479113740523448 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 3802 0.6933818688483332 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3453 0.6297337172891359 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 3354 0.6116787975058678 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2998 0.5467540354569445 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 2278 0.41544552794226797 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2264 0.412892306962816 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 1937 0.35325635979990044 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 1617 0.2948970231267109 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1254 0.2286956505880615 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 1235 0.22523056497309088 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1091 0.1989688634701556 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1051 0.19167394638600688 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAA 1021 0.18620275857289537 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAA 839 0.15301088584001882 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 828 0.15100478364187794 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 827 0.1508224107147742 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 759 0.13842105167172145 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 724 0.13203799922309134 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 684 0.12474308213894264 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAA 654 0.11927189432583113 No Hit CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC 620 0.11307121480430474 No Hit AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT 620 0.11307121480430474 No Hit TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT 598 0.10905901040802295 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 580 0.10577629772015604 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAA 576 0.10504680601174116 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAA 553 0.10085222868835567 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 552 0.10066985576125197 Illumina Single End Adapter 1 (100% over 32bp) TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 551 0.10048748283414824 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10760002699119321 0.0 0.0 0.0 0.0 2 0.23635531352641764 0.0 0.0 0.0 0.0 3 0.3271770312240688 0.0 0.0 0.0 0.0 4 0.39447264132534055 0.0 0.0 0.0 0.0 5 0.4362360416320918 0.0 0.0 0.0 0.0 6 0.5079086019838527 0.0 0.0 0.0 0.0 7 0.5649913281673162 0.0 0.0 0.0 0.0 8 0.6253567670386466 0.0 0.0 0.0 0.0 9 0.6817100015136953 0.0 0.0 0.0 0.0 10 0.7413459486766109 0.0 0.0 0.0 0.0 11 0.8042646085273933 0.0 0.0 0.0 0.0 12 0.8613473347108569 0.0 0.0 0.0 0.0 13 0.9102232791746531 0.0 0.0 0.0 0.0 14 0.9539927816795453 0.0 0.0 0.0 0.0 15 0.9990388946741634 0.0 0.0 0.0 0.0 16 1.03861381985567 0.0 0.0 0.0 0.0 17 1.0716233196614429 0.0 0.0 0.0 0.0 18 1.103173836050386 0.0 0.0 0.0 0.0 19 1.1274294353551804 0.0 0.0 0.0 0.0 20 1.1564267307646714 0.0 0.0 0.0 0.0 21 1.181958940559192 0.0 0.0 0.0 0.0 22 1.2080382691350235 0.0 0.0 0.0 0.0 23 1.2275521723351213 0.0 0.0 0.0 0.0 24 1.247066075535219 0.0 0.0 0.0 0.0 25 1.2696803184960799 0.0 0.0 0.0 0.0 26 1.293571171946667 0.0 0.0 0.0 0.0 27 1.3130850751467646 0.0 0.0 0.0 0.0 28 1.3442708456815002 0.0 0.0 0.0 0.0 29 1.3725386493825764 0.0 0.0 0.0 0.0 30 1.4029949282088972 0.0 0.0 0.0 0.0 31 1.4248796794613434 0.0 0.0 0.0 0.0 32 1.4511413809642786 0.0 0.0 0.0 0.0 33 1.4748498614877619 0.0 0.0 0.0 0.0 34 1.4983759690841414 0.0 0.0 0.0 0.0 35 1.5169780076487205 0.0 0.0 0.0 0.0 36 1.5364919108488184 0.0 0.0 0.0 0.0 37 1.5543644577049827 0.0 0.0 0.0 0.0 38 1.5645773416227908 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATGC 10 0.002568069 203.5365 44 CTAGATA 10 0.002568069 203.5365 44 GACACGA 10 0.002568069 203.5365 44 GACGACG 20 3.9186125E-7 203.5365 44 TACGGAT 15 3.1750795E-5 203.53648 44 CCCGTTC 15 3.1750795E-5 203.53648 44 AGTAGGC 15 3.1750795E-5 203.53648 44 CAGACTA 30 6.002665E-11 203.53648 44 CTCCGCG 35 1.7826096E-10 174.45985 44 GAATATT 30 1.4455509E-8 169.61374 44 TACGGAC 30 1.4455509E-8 169.61374 44 ATCGAAA 55 0.0 166.52986 44 GGACACT 25 1.190996E-6 162.8292 44 TGGTAAC 25 1.190996E-6 162.8292 44 CTGTACG 40 4.5110937E-10 152.65237 44 CTATCAT 400 0.0 147.56395 44 CACGCTA 15 0.008635419 135.691 44 GCTCGGC 30 2.9515068E-6 135.691 44 CCGCATC 15 0.008635419 135.691 44 GCATCAT 30 2.9515068E-6 135.691 44 >>END_MODULE