FastQCFastQC Report
Tue 24 May 2016
ERR841014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894978
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC142461.5917709709065475No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT102151.1413688381166913No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA90391.0099689601308635No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC58080.6489544994402097No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT55960.6252667663339211No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA38340.4283904185354277No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA31080.34727110610540146No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA22140.24738038253454275No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC19670.219781938773914No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC18360.20514470746766958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA17190.19207176042316124No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC16810.18782584599844912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA16600.18547941960584508No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC15200.16983657698848462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA14360.16045087141806838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA14070.15721056830447228No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA13900.15531108027236423No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT13150.146930986013064No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA12720.14212639863773185No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG11870.13262895847719164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11770.1315116125759516No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCCACCATCTTTGCAGGCAC11390.12726569815123948No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA11300.12626008684012344No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT11290.12614835224999943No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11240.12558967929937942No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAACTAATTAACCACTC11110.12413712962776738No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA10740.12000294979317928No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT10480.1170978504499552No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG10440.11665091208945919No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA10240.11441622028697913No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT10240.11441622028697913No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC9580.10704173733879492No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC9470.10581265684743088No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.10536571848693488No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT9330.10424837258569485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9210.10290755750420681No Hit
AAAAAAAAAAAAAAAAAAAAAAAA9180.10257235373383479No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT9160.1023488845535868No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT9150.1022371499634628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG204.157082E-7201.2182944
AGGGCGG100.002659215201.2182944
TCTGGCA201.04747654E-4150.9137144
CTATCAT13950.0144.9637144
ATTAGTA150.00894151134.1455244
TAACCGC150.00894151134.1455244
ACCGCCC1050.0114.9818844
GGATACG206.806971E-6114.7776143
GTTCGGC800.0114.7776143
TACCCCA401.3084573E-5100.60914644
ACGACAC2100.095.8182444
ACAGAGT304.4296394E-795.6480143
GATCGTC252.0623209E-591.8220943
CGGTTCC252.0623209E-591.8220943
CTTGTCC555.7714897E-791.4628644
CTTGTAC555.7714897E-791.4628644
ACCACTC2600.089.0003944
CCTATCA13050.088.8317243
ACTTCTA6950.088.3044344
AGACTAA702.4001565E-886.2364144
Produced by FastQC (version 0.11.5)