##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841014.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894978
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.84496825620295	39.0	37.0	39.0	32.0	39.0
2	37.5756007410238	39.0	37.0	39.0	35.0	39.0
3	37.83750438558266	39.0	38.0	39.0	35.0	39.0
4	38.30420971241751	39.0	38.0	40.0	35.0	41.0
5	38.95330834947898	40.0	38.0	41.0	35.0	41.0
6	38.861496036774085	40.0	38.0	41.0	35.0	41.0
7	38.84444757301297	40.0	38.0	41.0	35.0	41.0
8	38.768862474831785	40.0	38.0	41.0	35.0	41.0
9	38.750906726198856	40.0	38.0	41.0	35.0	41.0
10	38.636913980008444	40.0	38.0	41.0	35.0	41.0
11	38.45429720060158	40.0	37.0	41.0	35.0	41.0
12	38.35908592166511	40.0	37.0	41.0	34.0	41.0
13	38.140832512084096	40.0	36.0	41.0	34.0	41.0
14	38.083802059938904	40.0	36.0	41.0	34.0	41.0
15	38.01643951024494	40.0	36.0	41.0	34.0	41.0
16	37.94286451734009	39.0	36.0	41.0	34.0	41.0
17	37.85847361611123	39.0	35.0	41.0	34.0	41.0
18	37.775624652226085	39.0	35.0	41.0	34.0	41.0
19	37.731638096131974	39.0	35.0	41.0	34.0	41.0
20	37.492246736791294	39.0	35.0	40.0	33.0	41.0
21	37.33266404313849	38.0	35.0	40.0	33.0	41.0
22	37.20212563884252	38.0	35.0	40.0	33.0	41.0
23	37.012933278806855	38.0	35.0	40.0	33.0	41.0
24	36.69371872828159	37.0	35.0	40.0	33.0	41.0
25	36.53642803119272	37.0	35.0	40.0	33.0	41.0
26	36.33056242466769	37.0	35.0	40.0	32.0	41.0
27	36.16844254632184	36.0	35.0	39.0	32.0	41.0
28	35.98885392245577	36.0	34.0	39.0	32.0	41.0
29	35.879912220571335	36.0	34.0	39.0	31.0	41.0
30	35.732806872455086	36.0	34.0	39.0	31.0	41.0
31	35.576282018025836	35.0	34.0	39.0	31.0	41.0
32	35.496764396238476	35.0	34.0	39.0	31.0	41.0
33	35.34427544429879	35.0	34.0	38.0	31.0	40.0
34	35.243700698235855	35.0	34.0	38.0	31.0	40.0
35	35.01030500084924	35.0	34.0	37.0	31.0	40.0
36	34.93991811231183	35.0	34.0	37.0	31.0	40.0
37	34.838575743406025	35.0	34.0	37.0	31.0	39.0
38	34.60830457943175	35.0	34.0	36.0	30.0	39.0
39	34.531983947475304	35.0	33.0	36.0	30.0	39.0
40	34.293990661679224	35.0	33.0	36.0	30.0	39.0
41	34.19305828339028	35.0	33.0	36.0	30.0	39.0
42	34.06762926839211	35.0	33.0	36.0	30.0	39.0
43	33.869219195796035	35.0	33.0	36.0	29.0	38.0
44	33.6359362809355	35.0	32.0	35.0	29.0	37.0
45	33.451472854515224	35.0	32.0	35.0	29.0	37.0
46	33.171222281921175	35.0	32.0	35.0	27.0	37.0
47	33.18031582072702	35.0	32.0	35.0	28.0	37.0
48	33.80533794322896	35.0	33.0	35.0	30.0	37.0
49	34.32044507150645	35.0	34.0	35.0	31.0	37.0
50	34.27874158251495	35.0	34.0	36.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	12.0
22	28.0
23	60.0
24	157.0
25	347.0
26	601.0
27	1271.0
28	2797.0
29	5457.0
30	10346.0
31	18215.0
32	32311.0
33	56568.0
34	93316.0
35	121810.0
36	150129.0
37	165069.0
38	121227.0
39	85986.0
40	29269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.98218503695063	16.85616853151698	35.1616464315324
2	22.23730639188896	22.23808853401983	34.607889802877835	20.91671527121337
3	22.809275758733737	24.16517500988851	27.697999280429244	25.327549950948512
4	19.603386898895838	25.484983988433235	32.64739468456208	22.264234428108846
5	16.072350381797094	27.622466697505416	30.51773339679858	25.787449523898907
6	19.91769630091466	26.8589842431881	26.647247194903116	26.576072260994128
7	22.37094096167727	25.79795257537057	22.64536111502182	29.185745347930343
8	21.02833812674725	27.383689878410415	26.516629459048158	25.071342535794177
9	20.88934029663299	29.209544815626753	28.007727564252978	21.893387323487282
10	27.77163237532096	22.807599739881873	26.70434357045648	22.71642431434069
11	23.67477189383426	23.95410836914427	24.365068191620352	28.006051545401117
12	22.99922456194454	27.450060224944078	23.737678468074076	25.81303674503731
13	23.249063105461808	24.310876915410212	25.97527536989736	26.46478460923062
14	19.110078683498365	25.90745247369209	24.353782998017827	30.628685844791715
15	21.933723510522046	32.48102187986744	24.86854425471911	20.716710354891404
16	20.127198657397166	33.94943786327709	22.60346064372532	23.319902835600427
17	19.99758653285332	37.14817570934705	19.712439858856865	23.141797898942766
18	19.782385712274493	29.755926961333127	21.339407225652472	29.122280100739907
19	22.339767011032674	25.86030047665976	23.341691080674607	28.458241431632956
20	22.370829227087146	22.912630254598437	27.78727521793832	26.9292653003761
21	19.77445255637569	33.35713280102975	23.070064292083156	23.79835035051141
22	24.732674993128324	26.233270538493684	23.45029710227514	25.583757366102855
23	24.985865574349315	26.203325668340447	26.859766385318967	21.951042371991267
24	23.330182417891837	25.90622339320073	25.71214041015533	25.0514537787521
25	22.728340355329095	26.734642732318676	23.857328929461254	26.679687982890982
26	24.050657265571505	32.43468370332226	25.00200886162622	18.512650169480015
27	23.63611074374889	28.94117593407546	22.513236440913715	24.909476881261945
28	22.309286979877502	25.31852095437283	24.259793060549164	28.112399005200505
29	22.585456776634526	29.542626029245117	25.589188098278626	22.282729095841727
30	24.881183683066027	28.973869183322787	25.652861008011545	20.492086125599638
31	24.505138448042484	30.757231322130462	22.845341910776355	21.892288319050696
32	20.92246195818787	30.57176196032672	24.63395969932531	23.871816382160098
33	20.824848408665098	25.320451811015136	29.25338648757041	24.601313292749357
34	24.45777094587106	30.70237997715916	23.252121451598136	21.587727625371645
35	19.478805231359036	26.58481547084658	24.473831072719772	29.462548225074613
36	20.238340767498762	26.17086580060165	27.32473777023852	26.266055661661063
37	18.529155114709553	35.43655659016908	23.024507944923705	23.009780350197655
38	25.246686871554935	26.312937095310694	23.007154011863683	25.433222021270684
39	20.49379771881253	32.39308563954853	25.438233675772288	21.674882965866647
40	19.057833264295002	29.39130717719157	26.46867057202533	25.08218898648809
41	20.04180691771868	30.816119029725854	24.267463229489667	24.874610823065797
42	19.37382477489184	31.857836617593854	22.92230392531556	25.84603468219875
43	18.942588722476277	25.855122613450156	25.17040568607708	30.03188297799649
44	21.87532015835196	27.126705212637685	20.942362302355576	30.055612326654774
45	21.55786194439995	26.559159978568626	30.568428494887957	21.314549582143467
46	19.6745907997888	31.638674394154524	25.559084159527306	23.127650646529364
47	18.86676954543955	33.84387840755389	26.078302720178293	21.211049326828267
48	23.599097033769095	26.07062705256992	23.296792012829314	27.03348390083167
49	17.879817866991598	29.843840184698262	20.751619316359903	31.524722631950237
50	17.121983445381417	33.28577535238244	26.21869639318052	23.373544809055634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21448.0
1	19304.5
2	14892.0
3	12400.5
4	11334.0
5	7586.0
6	3494.0
7	2336.0
8	2164.5
9	1811.5
10	1534.5
11	1429.5
12	1476.5
13	1465.0
14	1555.5
15	1741.0
16	1819.0
17	2205.0
18	2725.0
19	2648.5
20	2314.0
21	2484.0
22	3189.0
23	4335.0
24	5410.5
25	6356.5
26	7822.5
27	9614.5
28	10878.0
29	11887.5
30	13400.0
31	17343.0
32	23748.5
33	26399.0
34	28330.0
35	33187.5
36	35123.0
37	35406.0
38	36144.5
39	37586.5
40	40329.5
41	43641.5
42	46454.0
43	46996.5
44	47950.0
45	52578.0
46	61145.5
47	67511.0
48	74177.5
49	83988.5
50	95673.0
51	103747.5
52	96690.5
53	82448.5
54	72049.0
55	64427.0
56	61223.0
57	61599.0
58	59352.0
59	51412.0
60	41320.5
61	33742.0
62	27598.0
63	21579.0
64	17086.0
65	14367.0
66	10024.5
67	6325.5
68	4736.5
69	3469.5
70	2442.0
71	1620.0
72	1176.5
73	893.5
74	641.5
75	446.5
76	319.5
77	239.5
78	148.5
79	97.0
80	73.0
81	49.5
82	27.5
83	22.0
84	19.0
85	14.5
86	13.0
87	10.5
88	5.5
89	3.0
90	1.5
91	1.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5155.0
25	6238.0
26	6461.0
27	7401.0
28	7562.0
29	7270.0
30	6775.0
31	6542.0
32	6269.0
33	5213.0
34	5832.0
35	4845.0
36	4618.0
37	4762.0
38	4928.0
39	6034.0
40	7338.0
41	6779.0
42	9307.0
43	14307.0
44	37170.0
45	78339.0
46	100207.0
47	89795.0
48	143971.0
49	131729.0
50	180131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.990802612500916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.21221761453188	7.939376319518081
2	11.52109673193343	2.7629439358428627
3	4.5769991781487915	1.6464568110828324
4	2.471942351356573	1.1856229121839221
5	1.5128039063189238	0.9069866516045272
6	1.158217476731472	0.8332774287501572
7	0.8541614078419808	0.7169456588454359
8	0.74283035359237	0.7125705715600296
9	0.5800438492493751	0.6259672172650045
>10	6.320606843415125	18.16740839315072
>50	2.413730854933127	20.61327993950342
>100	1.552085072997697	29.910239275704836
>500	0.05426216650624859	4.502588921594162
>1k	0.024324419468318335	4.438982575366395
>5k	0.002806663784805962	2.2933060598487542
>10k+	0.0018711091898706413	2.744047328178857
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	14246	1.5917709709065475	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	10215	1.1413688381166913	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	9039	1.0099689601308635	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5808	0.6489544994402097	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5596	0.6252667663339211	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3834	0.4283904185354277	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3108	0.34727110610540146	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2214	0.24738038253454275	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1967	0.219781938773914	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	1836	0.20514470746766958	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	1719	0.19207176042316124	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1681	0.18782584599844912	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	1660	0.18547941960584508	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1520	0.16983657698848462	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	1436	0.16045087141806838	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1407	0.15721056830447228	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1390	0.15531108027236423	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1315	0.146930986013064	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1272	0.14212639863773185	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	1187	0.13262895847719164	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1177	0.1315116125759516	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCCACCATCTTTGCAGGCAC	1139	0.12726569815123948	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	1130	0.12626008684012344	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	1129	0.12614835224999943	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1124	0.12558967929937942	No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAACTAATTAACCACTC	1111	0.12413712962776738	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1074	0.12000294979317928	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1048	0.1170978504499552	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1044	0.11665091208945919	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1024	0.11441622028697913	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	1024	0.11441622028697913	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	958	0.10704173733879492	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	947	0.10581265684743088	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.10536571848693488	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	933	0.10424837258569485	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.10290755750420681	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	918	0.10257235373383479	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	916	0.1023488845535868	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	915	0.1022371499634628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03720761851129301	0.0	0.0	0.0	0.0
2	0.07340962571147	0.0	0.0	0.0	0.0
3	0.09732082799800665	0.0	0.0	0.0	0.0
4	0.11832693094131923	0.0	0.0	0.0	0.0
5	0.13989170683525182	0.0	0.0	0.0	0.0
6	0.1765406523959248	0.0	0.0	0.0	0.0
7	0.20391562697630555	0.0	0.0	0.0	0.0
8	0.23687733106288647	0.0	0.0	0.0	0.0
9	0.26279975597165517	0.0	0.0	0.0	0.0
10	0.2884987117001759	0.0	0.0	0.0	0.0
11	0.31386246365832454	0.0	0.0	0.0	0.0
12	0.34693590233502947	0.0	0.0	0.0	0.0
13	0.3711823083919381	0.0	0.0	0.0	0.0
14	0.39453483772785475	0.0	0.0	0.0	0.0
15	0.42079246640699547	0.0	0.0	0.0	0.0
16	0.44515060705402815	0.0	0.0	0.0	0.0
17	0.4796765954023451	0.0	0.0	0.0	0.0
18	0.5171076830938861	0.0	0.0	0.0	0.0
19	0.5442591884940189	0.0	0.0	0.0	0.0
20	0.5692877366817956	0.0	0.0	0.0	0.0
21	0.5896234320843641	0.0	0.0	0.0	0.0
22	0.6094004545363126	0.0	0.0	0.0	0.0
23	0.6315239033808653	0.0	0.0	0.0	0.0
24	0.6547646981266578	0.0	0.0	0.0	0.0
25	0.6743182513983584	0.0	0.0	0.0	0.0
26	0.6974473115540271	0.0	0.0	0.0	0.0
27	0.7239284094134157	0.0	0.0	0.0	0.0
28	0.7471692041592084	0.0	0.0	0.0	0.0
29	0.7768906051321932	0.0	0.0	0.0	0.0
30	0.80471251807307	0.0	0.0	0.0	0.0
31	0.8307466775719626	0.0	0.0	0.0	0.0
32	0.8600211401844514	0.0	0.0	0.0	0.0
33	0.8830384657499961	0.0	0.0	0.0	0.0
34	0.9113073170513689	0.0	0.0	0.0	0.0
35	0.9343246426169135	0.0	0.0	0.0	0.0
36	0.9570067644120861	0.0	0.0	0.0	0.0
37	0.9749960334220505	0.0	0.0	0.0	0.0
38	0.987286838335691	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTCG	20	4.157082E-7	201.21829	44
AGGGCGG	10	0.002659215	201.21829	44
TCTGGCA	20	1.04747654E-4	150.91371	44
CTATCAT	1395	0.0	144.96371	44
ATTAGTA	15	0.00894151	134.14552	44
TAACCGC	15	0.00894151	134.14552	44
ACCGCCC	105	0.0	114.98188	44
GGATACG	20	6.806971E-6	114.77761	43
GTTCGGC	80	0.0	114.77761	43
TACCCCA	40	1.3084573E-5	100.609146	44
ACGACAC	210	0.0	95.81824	44
ACAGAGT	30	4.4296394E-7	95.64801	43
GATCGTC	25	2.0623209E-5	91.82209	43
CGGTTCC	25	2.0623209E-5	91.82209	43
CTTGTCC	55	5.7714897E-7	91.46286	44
CTTGTAC	55	5.7714897E-7	91.46286	44
ACCACTC	260	0.0	89.00039	44
CCTATCA	1305	0.0	88.83172	43
ACTTCTA	695	0.0	88.30443	44
AGACTAA	70	2.4001565E-8	86.23641	44
>>END_MODULE