FastQCFastQC Report
Tue 24 May 2016
ERR841013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163336
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC63310.544210786909371No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA42140.3622341266839503No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT24310.2089680023656106No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT23480.20183334823301266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA22450.1929795003335236No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA22140.19031474999484244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA20870.17939786957508408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA20740.1782803936266049No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA20180.17346665107930986No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT19770.16994230385718312No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA19620.16865290853201484No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA17460.15008561584959118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17230.14810854301766643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA17220.1480225833293219No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG16740.14389651828878328No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG15810.13590226727273977No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG15730.13521458976598336No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15540.13358135568743681No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC15410.13246387973895762No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC14740.1267045806198725No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC14310.12300831402105668No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA14130.12146103963085471No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA13770.11836649085045078No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC13690.11767881334369433No Hit
AAAAAAAAAAAAAAAAAAAAAAAA13320.11449830487494585No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT13260.11398254674487854No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT12660.10882496544420528No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA12410.10667597323559144No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA12220.1050427391570449No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC12120.10418314227359937No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12110.10409718258525481No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT12020.10332354539015383No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG11990.10306566632512018No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG11680.100400915986439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCA151.6065416E-5241.5194144
TCCTCTA301.8189894E-11241.5194144
CACCGTT151.6065416E-5241.5194144
CGTACGC100.0015398074241.519444
CATCGTA401.382432E-10181.1395444
CGTGCGC550.0175.6504744
TACGGCC150.0051807575161.0129444
CAGCCGT251.2314435E-4144.9116444
TCTATAG506.511982E-10144.9116444
ACGACAC1000.0132.8356644
TACGGTG152.247511E-4124.6151343
GTTCACT152.247511E-4124.6151343
TAGTGGT654.0381565E-9111.4704944
CGGCCTA706.7593646E-9103.50831644
CTATCAT4550.0103.5083144
TCGTGCG2650.098.7516143
ATAGGTC207.057857E-493.4613443
CCCGGTT655.2641735E-792.89207544
CTCGACA407.990745E-490.5697744
TCATAGC407.990745E-490.5697744
Produced by FastQC (version 0.11.5)