##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841013.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1163336
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.19653651223722	39.0	35.0	39.0	31.0	39.0
2	36.97278430307323	39.0	35.0	39.0	33.0	39.0
3	37.21446426483836	39.0	35.0	39.0	33.0	39.0
4	37.8061780947207	39.0	35.0	40.0	34.0	41.0
5	38.46547772956394	40.0	36.0	41.0	35.0	41.0
6	38.45612273668141	40.0	36.0	41.0	35.0	41.0
7	38.46195596113247	40.0	36.0	41.0	35.0	41.0
8	38.41879646121155	40.0	36.0	41.0	35.0	41.0
9	38.40450136503985	40.0	36.0	41.0	35.0	41.0
10	38.362544441158875	40.0	36.0	41.0	34.0	41.0
11	38.325334211268284	40.0	36.0	41.0	34.0	41.0
12	38.255815172916506	40.0	35.0	41.0	34.0	41.0
13	38.16255578783773	40.0	35.0	41.0	34.0	41.0
14	38.15348360232985	40.0	35.0	41.0	34.0	41.0
15	38.035832296086426	40.0	35.0	41.0	34.0	41.0
16	37.98128915463804	40.0	35.0	41.0	34.0	41.0
17	37.95467345633592	40.0	35.0	41.0	34.0	41.0
18	37.92777495065914	40.0	35.0	41.0	34.0	41.0
19	37.88453378903429	39.0	35.0	41.0	34.0	41.0
20	37.815098131580214	39.0	35.0	41.0	34.0	41.0
21	37.697839661112525	39.0	35.0	41.0	33.0	41.0
22	37.65326956270587	39.0	35.0	41.0	33.0	41.0
23	37.49787507650412	39.0	35.0	41.0	33.0	41.0
24	37.38664839736757	39.0	35.0	41.0	33.0	41.0
25	37.273495286502154	38.0	35.0	41.0	33.0	41.0
26	37.13018150104577	38.0	35.0	40.0	33.0	41.0
27	37.010578370129885	38.0	35.0	40.0	33.0	41.0
28	36.8520109432355	38.0	35.0	40.0	32.0	41.0
29	36.77260878247784	38.0	35.0	40.0	32.0	41.0
30	36.742461232018236	38.0	35.0	40.0	32.0	41.0
31	36.68072882736895	38.0	35.0	40.0	32.0	41.0
32	36.54685127231802	37.0	35.0	40.0	32.0	41.0
33	36.4463139586195	37.0	35.0	40.0	32.0	41.0
34	36.34449629400836	37.0	34.0	40.0	31.0	41.0
35	36.246717452053744	37.0	34.0	40.0	31.0	41.0
36	36.19400782763793	36.0	34.0	40.0	31.0	41.0
37	36.139870756847145	36.0	34.0	40.0	31.0	41.0
38	35.973712757206336	36.0	34.0	40.0	31.0	41.0
39	35.84808635091632	36.0	34.0	40.0	31.0	41.0
40	35.64119624381293	35.0	34.0	39.0	30.0	41.0
41	35.502233269933306	35.0	34.0	39.0	30.0	41.0
42	35.38096767666052	35.0	34.0	39.0	30.0	41.0
43	35.25658381535083	35.0	33.0	39.0	30.0	41.0
44	35.0417150481778	35.0	33.0	39.0	29.0	41.0
45	34.82730300171378	35.0	33.0	39.0	29.0	41.0
46	34.55783750580441	35.0	33.0	39.0	28.0	41.0
47	34.65091733401738	35.0	33.0	38.0	29.0	40.0
48	35.4682547155565	35.0	34.0	39.0	31.0	41.0
49	35.971271095896675	36.0	34.0	39.0	31.0	41.0
50	36.32930521466798	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	14.0
22	41.0
23	84.0
24	238.0
25	534.0
26	999.0
27	2084.0
28	3944.0
29	7506.0
30	13756.0
31	25276.0
32	47171.0
33	80419.0
34	129189.0
35	77691.0
36	116688.0
37	177641.0
38	169094.0
39	145805.0
40	165159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.56444913593321	18.599613525241203	34.83593733882559
2	24.878796839434177	21.874591691480365	31.119470213248796	22.127141255836662
3	21.46516569589525	21.180982966228157	28.4621983674536	28.891652970422992
4	17.68448668312508	24.53057414195039	33.79634086798655	23.988598306937977
5	18.02763775899654	26.208163419682705	32.97491008616599	22.789288735154763
6	21.038289883576198	28.749733524966132	28.087328166583	22.12464842487467
7	27.333117861047885	26.325154555519642	23.34304104747038	22.998686535962097
8	25.294583851956787	24.884556138553265	27.906984740436126	21.913875269053825
9	24.95074509857857	25.799854900046075	26.522002241828673	22.727397759546683
10	25.275930599586015	21.773675017363857	27.945752559879516	25.004641823170605
11	24.577851970539896	24.799971805222224	27.112889139509132	23.509287084728744
12	23.759171898746363	25.07229209789777	27.756383366456465	23.4121526368994
13	24.24303898443786	25.029398213413838	26.99624184242558	23.73132095972273
14	22.480177695867745	26.083521871583105	24.74770831470873	26.688592117840415
15	22.04118156749211	30.742365060481237	24.51449968022996	22.701953691796696
16	22.80304228528989	32.675856330415286	22.23656793909928	22.284533445195542
17	23.42461679170936	31.30213455098097	21.659950349684014	23.613298307625655
18	23.11430231678552	26.6565291540879	23.896793359785995	26.332375169340587
19	23.222009806281246	26.162089026730023	23.669429984114647	26.946471182874077
20	23.806707606400902	24.654098214101513	26.35928055179243	25.179913627705155
21	24.346276570139665	29.880361305762047	23.934357743592567	21.83900438050572
22	25.888307419352618	28.463831601532146	21.736712351375697	23.911148627739536
23	25.16616007757002	26.62205931906173	23.56223825274899	24.649542350619253
24	25.839310396996222	28.688874065618187	22.742698584072013	22.729116953313575
25	26.537658694678445	26.5276281360628	23.678171256223703	23.256541913035058
26	26.441811824854494	29.377701996398354	23.53037942622587	20.650106752521282
27	25.64893203951572	29.175318363451026	22.77483033325065	22.4009192637826
28	25.970743811292813	26.737318165028658	23.379413214946567	23.912524808731966
29	24.588299063554214	28.227992097624295	24.075678798382437	23.108030040439054
30	27.748994171769215	27.601131129799484	24.63326370137598	20.016610997055324
31	24.461191448744913	28.840162082612775	24.674228828276267	22.02441764036605
32	23.287447053413313	27.921513342128435	26.720552646712214	22.07048695774604
33	23.5467582753179	26.93574404852384	27.672556189065368	21.84494148709289
34	22.516716614770726	29.079442992842164	26.094707541560812	22.3091328508263
35	22.790681606620836	27.833989858483736	24.57370992574528	24.801618609150147
36	22.58575039283772	28.178087685699765	24.775685669712	24.460476251750514
37	24.708588812153394	29.063742949729328	23.408410252534317	22.819257985582965
38	24.211354219504273	27.46776549887248	24.243175518687178	24.07770476293607
39	22.88737311040423	29.436186512164376	23.578750451896244	24.09768992553515
40	22.014594544585446	29.29885664502799	23.493612477449	25.192936332937567
41	22.45489436842387	31.027226338089147	24.040900312784267	22.476978980702718
42	21.603966892790556	31.227689666079257	23.92748318805217	23.240860253078015
43	23.157526235808	27.01969973028237	25.928298073786472	23.894475960123163
44	23.62739106520809	28.392613416447677	23.721098413086438	24.258897105257798
45	23.359141032077137	26.45065521837727	27.63829262381637	22.551911125729223
46	22.35254973509958	29.211928867503477	26.96987313786644	21.465648259530504
47	22.726624230991494	30.76970744171099	24.159801012518862	22.343867314778652
48	24.773171828003218	27.9166984975243	23.70525395694573	23.604875717526753
49	20.58604273138542	28.6530113543088	24.570681177790878	26.190264736514905
50	21.007185996946344	32.846624040520894	25.408413713320932	20.737776249211834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28406.0
1	25979.0
2	20291.5
3	16046.0
4	13744.5
5	9236.0
6	4709.0
7	3423.5
8	3367.0
9	3078.5
10	2608.0
11	2246.5
12	2227.0
13	2047.0
14	1735.5
15	1770.5
16	2058.5
17	2471.5
18	2873.0
19	3268.0
20	4023.5
21	5053.0
22	5723.5
23	6961.5
24	8744.5
25	9931.5
26	10934.5
27	12315.5
28	13725.0
29	15819.5
30	19637.0
31	24193.5
32	28293.0
33	31759.0
34	38930.0
35	46086.5
36	45624.5
37	42354.0
38	41357.5
39	44482.0
40	49419.5
41	54854.5
42	60054.5
43	61903.0
44	63673.5
45	65276.5
46	71292.0
47	79227.0
48	83246.5
49	89568.5
50	100535.0
51	105993.5
52	102997.0
53	99558.0
54	94634.0
55	89000.0
56	86730.5
57	85906.0
58	81430.5
59	74705.5
60	68110.5
61	60131.5
62	53773.5
63	45939.0
64	34473.5
65	26472.5
66	19860.0
67	14129.5
68	10454.5
69	7348.5
70	5424.5
71	4232.5
72	3478.5
73	2653.0
74	1701.5
75	1194.5
76	876.5
77	514.0
78	258.0
79	184.0
80	153.5
81	128.5
82	84.0
83	42.0
84	26.0
85	22.5
86	20.0
87	19.0
88	15.5
89	7.5
90	6.0
91	11.0
92	11.0
93	11.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	6870.0
25	7547.0
26	8006.0
27	9255.0
28	9970.0
29	9172.0
30	10362.0
31	8363.0
32	8304.0
33	7368.0
34	7076.0
35	6354.0
36	6219.0
37	5716.0
38	7160.0
39	7946.0
40	9783.0
41	9839.0
42	12530.0
43	23716.0
44	57508.0
45	103775.0
46	126584.0
47	131042.0
48	191294.0
49	179676.0
50	191901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.313116693967528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.67520533252312	9.142810225740613
2	11.337253099259469	3.0186834699897265
3	4.262797541366286	1.7025336333290169
4	2.2318449772843514	1.1885125050172747
5	1.4241020177074035	0.9479618172926637
6	1.020770472836354	0.8153781853576075
7	0.7790845304078715	0.7260430287449419
8	0.5895045534344995	0.6278514329198964
9	0.4843841537484662	0.5803796487205836
>10	5.366649681825775	17.039049699006306
>50	2.267080763975613	21.73186215085739
>100	1.484754802838654	31.700224860908666
>500	0.04727246233253113	4.298506844816569
>1k	0.028629801130969558	5.9190228289988704
>5k	6.65809328627199E-4	0.561179668299866
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	6331	0.544210786909371	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4214	0.3622341266839503	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2431	0.2089680023656106	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2348	0.20183334823301266	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	2245	0.1929795003335236	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	2214	0.19031474999484244	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2087	0.17939786957508408	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2074	0.1782803936266049	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2018	0.17346665107930986	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1977	0.16994230385718312	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1962	0.16865290853201484	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1746	0.15008561584959118	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1723	0.14810854301766643	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1722	0.1480225833293219	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	1674	0.14389651828878328	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1581	0.13590226727273977	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1573	0.13521458976598336	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1554	0.13358135568743681	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1541	0.13246387973895762	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1474	0.1267045806198725	No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC	1431	0.12300831402105668	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	1413	0.12146103963085471	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	1377	0.11836649085045078	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	1369	0.11767881334369433	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	1332	0.11449830487494585	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	1326	0.11398254674487854	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	1266	0.10882496544420528	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1241	0.10667597323559144	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1222	0.1050427391570449	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	1212	0.10418314227359937	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1211	0.10409718258525481	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	1202	0.10332354539015383	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	1199	0.10306566632512018	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1168	0.100400915986439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06584512127192832	0.0	0.0	0.0	0.0
2	0.11630345833018148	0.0	0.0	0.0	0.0
3	0.153008245253306	0.0	0.0	0.0	0.0
4	0.18214857960210978	0.0	0.0	0.0	0.0
5	0.21051527675581258	0.0	0.0	0.0	0.0
6	0.25349512092808957	0.0	0.0	8.595968834455393E-5	0.0
7	0.28504232655054085	0.0	0.0	8.595968834455393E-5	0.0
8	0.3153001368478238	0.0	0.0	8.595968834455393E-5	0.0
9	0.34813673779544346	0.0	0.0	8.595968834455393E-5	0.0
10	0.380027782171273	0.0	0.0	8.595968834455393E-5	0.0
11	0.4114890281053797	0.0	0.0	8.595968834455393E-5	0.0
12	0.4420906771560409	0.0	0.0	8.595968834455393E-5	0.0
13	0.4719186890116011	0.0	0.0	8.595968834455393E-5	0.0
14	0.49701891800821085	0.0	0.0	8.595968834455393E-5	0.0
15	0.5241821795250899	0.0	0.0	8.595968834455393E-5	0.0
16	0.5522909976137591	0.0	0.0	8.595968834455393E-5	0.0
17	0.5834084047944875	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
18	0.6075630772193072	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
19	0.6337807821643962	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
20	0.6546689864321228	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
21	0.6863021517429186	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
22	0.7084797513358135	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
23	0.733064222202356	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
24	0.7592819271474449	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
25	0.7862732692876349	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
26	0.8148978455063713	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
27	0.8392244373078801	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
28	0.8634650694210443	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
29	0.8930352022115708	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
30	0.9219176574953409	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
31	0.9487370802588418	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
32	0.9771897371008892	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
33	1.0059002730079702	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
34	1.0298830260561007	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
35	1.0567884085079462	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
36	1.0787940887241518	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
37	1.1006278495636685	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
38	1.1136077625036962	0.0	0.0	8.595968834455393E-5	8.595968834455393E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGCA	15	1.6065416E-5	241.51941	44
TCCTCTA	30	1.8189894E-11	241.51941	44
CACCGTT	15	1.6065416E-5	241.51941	44
CGTACGC	10	0.0015398074	241.5194	44
CATCGTA	40	1.382432E-10	181.13954	44
CGTGCGC	55	0.0	175.65047	44
TACGGCC	15	0.0051807575	161.01294	44
CAGCCGT	25	1.2314435E-4	144.91164	44
TCTATAG	50	6.511982E-10	144.91164	44
ACGACAC	100	0.0	132.83566	44
TACGGTG	15	2.247511E-4	124.61513	43
GTTCACT	15	2.247511E-4	124.61513	43
TAGTGGT	65	4.0381565E-9	111.47049	44
CGGCCTA	70	6.7593646E-9	103.508316	44
CTATCAT	455	0.0	103.50831	44
TCGTGCG	265	0.0	98.75161	43
ATAGGTC	20	7.057857E-4	93.46134	43
CCCGGTT	65	5.2641735E-7	92.892075	44
CTCGACA	40	7.990745E-4	90.56977	44
TCATAGC	40	7.990745E-4	90.56977	44
>>END_MODULE