FastQCFastQC Report
Tue 24 May 2016
ERR841012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944646
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC542652.7904821751619577No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA360461.8536021466117742No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT207181.0653867079149624No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT182410.9380113398531146No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC109980.5655528049835291No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC70990.3650535881594902No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA67620.3477239559282255No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC59110.30396277780120395No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT58580.30123734602596053No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT56130.28863865197059No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG54130.2783540037621243No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA49170.2528480762051294No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT47350.2434890463354256No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC32890.16913103978821853No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC32280.1659942220846365No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC28540.1467619299348056No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT27470.14125964314327646No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT25050.12881521881103294No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGT24980.12845525612373668No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24540.1261926335178742No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG24130.12408428063513874No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTG23920.12300439257324984No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC23470.12069034672634504No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT23040.11847914736152491No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG23000.1182734543973556No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC22410.11523948317585823No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG21060.10829734563514387No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTT21040.10819449915305923No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.107063187850128No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.10608614627032374No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.10161232429964116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA257.3287083E-9190.1993644
CGATCTT450.0190.1993644
CTATCAT23750.0152.9603344
TACGGAC201.3121766E-4142.6495244
GCTCCGT201.3121766E-4142.6495244
CGACGTA253.1901264E-4114.1196244
CGAATGC253.1901264E-4114.1196244
GCGAATG153.2019653E-4114.0217643
GACCGCA1200.0110.9496244
AATCGCA2250.0101.4396644
TTTAACG504.5865454E-795.0996844
CTTAGCC14200.091.751144
CCTATCA24750.088.4532343
CTAAGGC402.5551344E-885.5163143
CATCGAC200.001004913285.5163143
ATCGATT200.001004913285.5163143
TGTTTTT17400.079.2497344
CCTTAGC92200.078.65275643
CAGGTAA800.078.3899543
ACGTACA150.001532050476.9019442
Produced by FastQC (version 0.11.5)