##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841012.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1944646
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.140141187650606	39.0	37.0	39.0	34.0	39.0
2	37.75426375803102	39.0	38.0	39.0	35.0	39.0
3	37.9446660214764	39.0	38.0	39.0	35.0	39.0
4	38.41069428574661	39.0	38.0	40.0	35.0	41.0
5	39.117968514577974	40.0	38.0	41.0	35.0	41.0
6	39.069835846729944	40.0	38.0	41.0	35.0	41.0
7	39.11725784538677	40.0	38.0	41.0	35.0	41.0
8	39.0645942757705	40.0	38.0	41.0	35.0	41.0
9	39.031838185458945	40.0	38.0	41.0	35.0	41.0
10	38.94953888779757	40.0	38.0	41.0	35.0	41.0
11	38.87258092218327	40.0	38.0	41.0	35.0	41.0
12	38.81765935805283	40.0	38.0	41.0	35.0	41.0
13	38.634591077244906	40.0	38.0	41.0	35.0	41.0
14	38.55163510479542	40.0	38.0	41.0	35.0	41.0
15	38.49229525579463	40.0	38.0	41.0	35.0	41.0
16	38.43389079554839	40.0	38.0	41.0	35.0	41.0
17	38.439193560164675	40.0	37.0	41.0	35.0	41.0
18	38.3629226090507	40.0	37.0	41.0	35.0	41.0
19	38.318033719247616	40.0	37.0	41.0	34.0	41.0
20	38.18376815111851	40.0	37.0	41.0	34.0	41.0
21	38.105379076705994	39.0	37.0	41.0	34.0	41.0
22	37.922074248989276	39.0	36.0	41.0	34.0	41.0
23	37.786416139492744	39.0	36.0	41.0	33.0	41.0
24	37.560326660996395	39.0	35.0	40.0	33.0	41.0
25	37.41420645472664	38.0	35.0	40.0	33.0	41.0
26	37.23674872171783	38.0	35.0	40.0	33.0	41.0
27	37.11344135429768	38.0	35.0	40.0	33.0	41.0
28	36.911056960899195	38.0	35.0	40.0	32.0	41.0
29	36.82623415023847	38.0	35.0	40.0	32.0	41.0
30	36.67671281893801	38.0	35.0	40.0	32.0	41.0
31	36.54433343715053	37.0	35.0	40.0	31.0	41.0
32	36.40051042805934	37.0	34.0	40.0	31.0	41.0
33	36.14093466289464	37.0	34.0	39.0	31.0	41.0
34	36.08607426691492	36.0	34.0	39.0	31.0	41.0
35	35.89995271344625	36.0	34.0	39.0	31.0	41.0
36	35.77868402504434	36.0	34.0	39.0	31.0	41.0
37	35.73995149548133	36.0	34.0	39.0	31.0	41.0
38	35.4601671280955	35.0	34.0	39.0	31.0	41.0
39	35.205458341224	35.0	34.0	38.0	30.0	40.0
40	34.98226340686346	35.0	33.0	38.0	30.0	40.0
41	34.84528114651005	35.0	33.0	37.0	30.0	40.0
42	34.79418623749655	35.0	33.0	37.0	30.0	40.0
43	34.46695269515289	35.0	33.0	37.0	29.0	39.0
44	34.23107699688175	35.0	33.0	37.0	29.0	39.0
45	34.05654352244207	35.0	32.0	36.0	29.0	39.0
46	33.60353273926681	35.0	32.0	36.0	27.0	39.0
47	33.484168281781095	35.0	32.0	36.0	28.0	39.0
48	34.26663151183645	35.0	33.0	36.0	30.0	39.0
49	34.89282897384306	35.0	34.0	37.0	31.0	39.0
50	34.80345112641605	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	23.0
22	45.0
23	85.0
24	198.0
25	412.0
26	823.0
27	1768.0
28	3989.0
29	8176.0
30	16349.0
31	30396.0
32	54206.0
33	92320.0
34	145230.0
35	193516.0
36	294893.0
37	373923.0
38	296158.0
39	303338.0
40	128790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.584693563764304	16.379793545971864	40.03551289026383
2	20.271504428055287	19.20714618496117	40.357988034840275	20.16336135214327
3	21.623215742093933	24.538296430301454	28.292347296114563	25.546140531490053
4	19.847262689456077	26.54848234588712	32.53106220875162	21.073192755905186
5	14.798786000125471	29.089150416065447	31.63645208433823	24.475611499470855
6	16.684373402665575	26.20065554347681	28.551006198557477	28.563964855300146
7	22.656102961670143	22.788877770041438	22.481726751295607	32.07329251699281
8	21.984926819585674	25.252873787825653	30.913389892042048	21.84880950054663
9	20.735856294667514	31.197554722041954	25.85437143829777	22.212217544992765
10	28.81151633767791	20.469483906068252	26.002470372499676	24.716529383754164
11	21.92789844526973	21.231062105905135	26.894663604584075	29.946375844241057
12	21.580380182305674	24.296504350920426	26.06618376815112	28.056931698622783
13	24.381815507809648	21.70240753329912	24.422388444992045	29.493388513899188
14	18.12360707295827	22.896866576230327	24.147274105415587	34.83225224539582
15	21.59282460663792	34.41721526694319	23.260480313640635	20.729479812778266
16	19.46241115349529	35.763218601226136	21.903009596605244	22.87136064867333
17	19.344600508267316	38.23708788129048	21.22669113041654	21.19162048002567
18	20.08324394259932	25.533027605024255	22.811658265823187	31.572070186553237
19	21.01230763851107	23.021876475204227	23.718198582158397	32.2476173041263
20	20.939955138364517	22.820554486523513	26.05497350160389	30.184516873508084
21	22.472162028461735	32.702044485217364	22.50383874494381	22.32195474137709
22	24.305143455415536	23.773118603591605	22.669781543787405	29.251956397205454
23	25.032473776718227	22.42824658061159	31.63002417920794	20.909255463462245
24	22.990919684096745	24.6254588238682	26.265294557467016	26.11832693456804
25	22.596452210147305	25.710857555653504	23.58082980188949	28.111860432309697
26	24.455381883712576	33.18195450734483	23.377658897088214	18.98500471185438
27	24.1323664557895	26.603246692825056	22.094061460708577	27.170325390676865
28	23.49522597272977	23.195276613530673	21.746749671255923	31.562747742483634
29	21.455363920550543	26.908907270241784	29.94202500314303	21.693703806064644
30	24.435958716637515	27.553894014446506	29.907045676061795	18.103101592854188
31	23.767036374208796	31.625137100552596	22.902233790935846	21.70559273430276
32	20.531694991865017	27.593628158199095	24.17780326244717	27.696873587488714
33	21.248000227063947	23.850120327517914	33.5717868677347	21.330092577683434
34	23.85301499947823	31.943945560297244	24.354022836993952	19.849016603230574
35	19.799184237815417	24.85939854821984	23.766830279986173	31.574586933978573
36	20.603126423163904	25.601138865165623	25.8623050996173	27.933429612053175
37	21.06793936431194	37.106516751753965	21.741414134626638	20.084129749307458
38	29.743743678349936	25.47943168788338	20.452185235328706	24.324639398437974
39	19.812597026908417	35.05085372339521	24.37582426803864	20.760724981657734
40	18.961676228611328	27.008851155346893	26.493871727669415	27.535600888372368
41	19.719430437441584	34.20323552150283	21.710937770444133	24.36639627061145
42	18.953699810009283	34.546625771873686	22.760037415706908	23.73963700241012
43	20.257837952139706	25.30368438382243	22.655741509412845	31.78273615462502
44	20.943151834972415	25.863036699448312	23.144279203645958	30.049532261933315
45	21.1127898904675	24.97862435723866	33.610237692121636	20.2983480601722
46	19.421240423233876	29.33603889963675	30.37121919990138	20.871501477227994
47	19.514179113769075	34.78769718575822	26.715583537038345	18.982540163434365
48	26.71896195929587	24.66081547289205	24.30926717075118	24.310955397060894
49	17.978562282787635	27.071959026888603	22.872654106456924	32.07682458386684
50	18.315018315018314	28.38888444447677	29.545239670984213	23.750857569520704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27174.0
1	23984.0
2	23245.5
3	24893.5
4	22324.0
5	15130.5
6	7403.0
7	5405.5
8	5764.0
9	5831.5
10	5600.0
11	4896.5
12	4285.5
13	4162.5
14	4541.5
15	5119.5
16	5596.0
17	5760.5
18	5907.5
19	6702.5
20	7403.0
21	7366.0
22	7436.5
23	9279.0
24	12274.5
25	14885.0
26	17005.0
27	19350.0
28	21930.0
29	24875.5
30	29284.5
31	43387.5
32	64550.0
33	69144.0
34	64502.5
35	66910.0
36	67957.0
37	67099.0
38	66823.0
39	69006.0
40	74125.0
41	81220.0
42	88051.5
43	92985.0
44	99435.5
45	108648.0
46	119987.5
47	124962.5
48	147324.0
49	190828.0
50	235286.5
51	260265.0
52	227347.0
53	175831.5
54	157675.5
55	152073.0
56	146228.5
57	145548.0
58	138740.0
59	115109.5
60	88719.5
61	73655.5
62	63032.5
63	50875.0
64	38452.5
65	29601.0
66	21588.0
67	15000.0
68	10916.5
69	7822.5
70	5541.0
71	3689.5
72	2568.0
73	2033.5
74	1578.5
75	1395.0
76	1332.0
77	1006.5
78	538.5
79	268.0
80	182.5
81	95.5
82	56.0
83	41.5
84	26.0
85	20.0
86	20.0
87	19.0
88	16.5
89	11.0
90	5.5
91	3.5
92	3.0
93	3.0
94	2.0
95	2.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	9413.0
25	11362.0
26	10909.0
27	13308.0
28	14531.0
29	12698.0
30	13388.0
31	13919.0
32	13035.0
33	11353.0
34	12605.0
35	11951.0
36	10361.0
37	10169.0
38	11958.0
39	13519.0
40	16907.0
41	16334.0
42	19193.0
43	30133.0
44	75835.0
45	164091.0
46	222909.0
47	197791.0
48	323599.0
49	270872.0
50	412503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.160185082299963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.99863042888246	7.5887730092924235
2	11.468150076402734	2.5597335480849392
3	4.435442296289384	1.4850107084535322
4	2.299530879462083	1.026527608690434
5	1.508905186036134	0.8419830573902755
6	1.0093066923936422	0.6758429695150225
7	0.7835336220363337	0.6121066168091243
8	0.6199670765183054	0.5535157855101369
9	0.5159400950804498	0.5182188257229534
>10	5.346201808191131	14.29313771079839
>50	2.273681126697706	18.371993787218994
>100	1.6419400480136312	30.29373827671224
>500	0.055252896195813084	4.350260771403945
>1k	0.03813916728560549	7.174546979190981
>5k	0.00293378209889273	2.00029050221504
>10k+	0.0024448184157439415	7.654319842991576
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	54265	2.7904821751619577	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	36046	1.8536021466117742	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	20718	1.0653867079149624	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	18241	0.9380113398531146	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	10998	0.5655528049835291	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	7099	0.3650535881594902	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	6762	0.3477239559282255	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	5911	0.30396277780120395	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	5858	0.30123734602596053	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	5613	0.28863865197059	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	5413	0.2783540037621243	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	4917	0.2528480762051294	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT	4735	0.2434890463354256	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	3289	0.16913103978821853	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3228	0.1659942220846365	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	2854	0.1467619299348056	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT	2747	0.14125964314327646	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	2505	0.12881521881103294	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGT	2498	0.12845525612373668	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2454	0.1261926335178742	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	2413	0.12408428063513874	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTG	2392	0.12300439257324984	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	2347	0.12069034672634504	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	2304	0.11847914736152491	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2300	0.1182734543973556	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2241	0.11523948317585823	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	2106	0.10829734563514387	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTT	2104	0.10819449915305923	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2082	0.107063187850128	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2063	0.10608614627032374	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.10161232429964116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02411750004885208	0.0	0.0	0.0	0.0
2	0.051268971319201544	0.0	0.0	0.0	0.0
3	0.07008987754069378	0.0	0.0	0.0	0.0
4	0.0948244564820538	0.0	0.0	0.0	0.0
5	0.11415959511396932	0.0	0.0	0.0	0.0
6	0.14804751096086383	0.0	0.0	0.0	0.0
7	0.17463332657974767	0.0	0.0	0.0	0.0
8	0.20564154092827178	0.0	0.0	0.0	0.0
9	0.23191881710090165	0.0	0.0	0.0	0.0
10	0.25192245786636747	0.0	0.0	0.0	0.0
11	0.271103326775156	0.0	0.0	0.0	0.0
12	0.29990034175886	0.0	0.0	0.0	0.0
13	0.3260233482083629	0.0	0.0	0.0	0.0
14	0.34854672778490275	0.0	0.0	0.0	0.0
15	0.37934924916925755	0.0	0.0	0.0	0.0
16	0.40567794858292977	0.0	0.0	0.0	0.0
17	0.4377146277523004	0.0	0.0	0.0	0.0
18	0.47273385490212616	0.0	0.0	0.0	0.0
19	0.49618285281742797	0.0	0.0	0.0	0.0
20	0.5194775810096027	0.0	0.0	0.0	0.0
21	0.5419495373451003	0.0	0.0	0.0	0.0
22	0.5619531781105661	0.0	0.0	0.0	0.0
23	0.5852993295437833	0.0	0.0	0.0	0.0
24	0.6059714724427994	0.0	0.0	0.0	0.0
25	0.6307574746252017	0.0	0.0	0.0	0.0
26	0.6559034394949004	0.0	0.0	0.0	0.0
27	0.6824378318727419	0.0	0.0	0.0	0.0
28	0.7102063820355993	0.0	0.0	0.0	0.0
29	0.7366893511723984	0.0	0.0	0.0	0.0
30	0.7642522083710865	0.0	0.0	0.0	0.0
31	0.7899124056512085	0.0	0.0	0.0	0.0
32	0.8161896818238383	0.0	0.0	0.0	0.0
33	0.8427240742016799	0.0	0.0	0.0	0.0
34	0.8698755454720294	0.0	0.0	0.0	0.0
35	0.8905476883710455	0.0	0.0	0.0	0.0
36	0.9113741009931885	0.0	0.0	0.0	0.0
37	0.9327147460257548	0.0	0.0	0.0	0.0
38	0.944953477393829	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTA	25	7.3287083E-9	190.19936	44
CGATCTT	45	0.0	190.19936	44
CTATCAT	2375	0.0	152.96033	44
TACGGAC	20	1.3121766E-4	142.64952	44
GCTCCGT	20	1.3121766E-4	142.64952	44
CGACGTA	25	3.1901264E-4	114.11962	44
CGAATGC	25	3.1901264E-4	114.11962	44
GCGAATG	15	3.2019653E-4	114.02176	43
GACCGCA	120	0.0	110.94962	44
AATCGCA	225	0.0	101.43966	44
TTTAACG	50	4.5865454E-7	95.09968	44
CTTAGCC	1420	0.0	91.7511	44
CCTATCA	2475	0.0	88.45323	43
CTAAGGC	40	2.5551344E-8	85.51631	43
CATCGAC	20	0.0010049132	85.51631	43
ATCGATT	20	0.0010049132	85.51631	43
TGTTTTT	1740	0.0	79.24973	44
CCTTAGC	9220	0.0	78.652756	43
CAGGTAA	80	0.0	78.38995	43
ACGTACA	15	0.0015320504	76.90194	42
>>END_MODULE