FastQCFastQC Report
Tue 24 May 2016
ERR841011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793858
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC377791.3522161827838064No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA246790.8833305057021509No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT175830.629344798482958No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT142840.5112643520178907No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC104050.3724240816820325No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA61220.2191235202361752No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA61170.2189445562372891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA55460.19850686756449323No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54610.1954644795834291No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA53040.18984501001840465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA50300.18003778287944483No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC42180.15097402946033764No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA41600.14889804707325854No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC40830.14614200149041218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40050.1433501631077886No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC38600.13816020714009086No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC36440.13042896238821014No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC35660.12763712400558655No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA35630.12752974560625488No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG31860.1140358600902408No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31580.11303366169647848No Hit
AAAAAAAAAAAAAAAAAAAAAAAA29850.10684150733501845No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28930.1035485697555137No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28840.10322643455751868No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG28680.10265374976108307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGC402.4556357E-10166.746244
CTATCAT28000.0149.6745644
CGAGTTA150.0066393395148.2188344
CGTTCTA251.7141149E-4133.3969644
CAATCGT354.9112714E-11120.6324143
AACTACC8300.0100.4495244
AATCGCA3250.082.0904444
CTATCGA150.001222442181.4036242
CTTAACC2200.080.8466444
AAGTGTA553.880651E-580.8466444
TCGTGCG2450.078.7803543
AGAGCCA6250.078.25954444
ACGAACA2850.078.0099244
CCTATCA29850.077.7947643
GTTCGGC800.075.39525643
TGCGTAG752.0226726E-674.1094244
ACCTTAG48550.072.6009742
TGTCGCA250.001948510572.3794543
ACTTCTA18050.070.2089244
ACAGCGT951.0021904E-770.2089244
Produced by FastQC (version 0.11.5)