##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841011.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2793858 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.189167094390626 39.0 35.0 39.0 31.0 39.0 2 37.00814894672528 39.0 37.0 39.0 33.0 39.0 3 37.29505866081956 39.0 37.0 39.0 33.0 39.0 4 37.87204933106837 39.0 37.0 40.0 34.0 41.0 5 38.532237500975356 40.0 37.0 41.0 35.0 41.0 6 38.530554881457824 40.0 36.0 41.0 35.0 41.0 7 38.49287938041232 40.0 36.0 41.0 35.0 41.0 8 38.45882360520828 40.0 36.0 41.0 35.0 41.0 9 38.46170420973435 40.0 36.0 41.0 35.0 41.0 10 38.4110702834575 40.0 36.0 41.0 35.0 41.0 11 38.31714460792209 40.0 36.0 41.0 34.0 41.0 12 38.24733110988461 40.0 36.0 41.0 34.0 41.0 13 38.09248394156038 40.0 36.0 41.0 34.0 41.0 14 38.010895686180184 40.0 35.0 41.0 34.0 41.0 15 37.939909616022 40.0 35.0 41.0 34.0 41.0 16 37.87388335412895 39.0 35.0 41.0 34.0 41.0 17 37.86300484849266 39.0 35.0 41.0 34.0 41.0 18 37.8229111858942 39.0 35.0 41.0 34.0 41.0 19 37.79017795464193 39.0 35.0 41.0 34.0 41.0 20 37.675497466227704 39.0 35.0 41.0 33.0 41.0 21 37.54476641260938 39.0 35.0 41.0 33.0 41.0 22 37.40612013924831 39.0 35.0 41.0 33.0 41.0 23 37.287825293912576 38.0 35.0 40.0 33.0 41.0 24 37.032727146476304 38.0 35.0 40.0 33.0 41.0 25 36.89145255780138 38.0 35.0 40.0 33.0 41.0 26 36.69216987132301 38.0 35.0 40.0 32.0 41.0 27 36.51941407173518 37.0 35.0 40.0 32.0 41.0 28 36.317470225563795 37.0 35.0 40.0 32.0 41.0 29 36.22444456430964 37.0 35.0 40.0 31.0 41.0 30 36.10669637233909 36.0 34.0 40.0 31.0 41.0 31 36.00393361862681 36.0 34.0 39.0 31.0 41.0 32 35.8611748518979 36.0 34.0 39.0 31.0 41.0 33 35.70158951831034 36.0 34.0 39.0 31.0 41.0 34 35.57803611046116 35.0 34.0 39.0 31.0 41.0 35 35.370477856992245 35.0 34.0 39.0 31.0 41.0 36 35.305340271540025 35.0 34.0 38.0 31.0 41.0 37 35.27245320597885 35.0 34.0 38.0 31.0 40.0 38 35.08205027308705 35.0 34.0 38.0 30.0 40.0 39 34.92998760050621 35.0 33.0 38.0 30.0 40.0 40 34.72815417069739 35.0 33.0 37.0 30.0 40.0 41 34.617945549539684 35.0 33.0 37.0 30.0 40.0 42 34.500003085161566 35.0 33.0 37.0 29.0 40.0 43 34.323103805105994 35.0 33.0 37.0 29.0 40.0 44 34.09580283054496 35.0 33.0 36.0 29.0 40.0 45 33.94605812878079 35.0 32.0 36.0 28.0 40.0 46 33.67880369943611 35.0 32.0 36.0 27.0 40.0 47 33.693086260425495 35.0 32.0 36.0 28.0 39.0 48 34.52575645419775 35.0 34.0 37.0 30.0 39.0 49 35.02352883435583 35.0 34.0 37.0 31.0 40.0 50 35.511646997215884 35.0 34.0 38.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 9.0 21 52.0 22 123.0 23 246.0 24 577.0 25 1243.0 26 2538.0 27 5146.0 28 10150.0 29 19480.0 30 35639.0 31 65393.0 32 119978.0 33 203518.0 34 325820.0 35 247644.0 36 375988.0 37 475286.0 38 394556.0 39 293912.0 40 216560.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 41.85924982586803 20.515681183510402 37.62506899062157 2 22.077714758588304 18.83438599957478 35.638425431786445 23.449473810050474 3 20.923826479370103 23.082812369132576 29.96347702710732 26.02988412439 4 18.571165750013062 24.750542081952627 34.16218719777454 22.51610497025976 5 16.668742649053744 26.085291378445145 32.58998846755991 24.655977504941195 6 20.98274142780342 25.363994877334495 27.7116804075225 25.941583287339586 7 24.984949127693675 23.342131203518576 23.090114100287128 28.582805568500618 8 24.64266258342407 24.13018127621375 27.64399622314377 23.583159917218413 9 24.02158592168965 26.651962984518185 25.396530532331994 23.929920561460175 10 27.23356018809832 22.19504355625805 25.552479760961365 25.018916494682266 11 25.441880009649736 23.04286760458119 25.56600943927716 25.949242946491914 12 23.784351244766196 25.439517684864445 25.377166627652514 25.398964442716842 13 25.951247343279437 23.339196193936843 24.636363050663277 26.073193412120443 14 21.19867938885942 25.138428653138423 23.96632183883361 29.69657011916855 15 23.26868437837571 31.538360217305243 22.28882069167438 22.904134712644666 16 21.29907819223454 32.27998702868936 21.212924923170757 25.208009855905345 17 22.147474925354118 32.72965197229065 20.476917581351664 24.645955521003575 18 21.942847489027717 27.002875593534103 21.830386512127674 29.223890405310506 19 23.816672142965032 25.21545475825901 21.86528449191047 29.10258860686549 20 23.565871994926013 24.333305414949507 25.22783906698193 26.87298352314255 21 24.412837016054503 30.845590577617045 21.766639535724437 22.974932870604018 22 25.75513859330002 25.94541311691575 21.651207756442883 26.64824053334135 23 26.960854846595637 25.54907944498253 25.54768352579122 21.942382182630613 24 25.63125971327104 26.528835753284525 23.185859839691208 24.654044693753228 25 25.061170960727075 26.523548524280955 23.44294473313977 24.972335781852202 26 26.24762578877479 31.38509615419482 22.462195740162844 19.905082316867542 27 27.45014066473267 27.8192051666107 20.90984265793677 23.82081151071986 28 25.99794412412535 26.059369683794785 21.326209541535253 26.616476650544605 29 24.682790188992282 28.514826808223333 24.5842485971122 22.218134405672185 30 25.482810897378222 29.083330334275036 24.757638601479133 20.676220166867605 31 25.437690180956285 28.93958953413318 22.602227224011813 23.02049306089872 32 22.141606633511298 27.955490138716204 24.760134023257514 25.14276920451498 33 23.83855435554106 24.907095858351397 29.618561645149533 21.63578814095801 34 26.119465223545546 27.61644855367417 24.675262998167526 21.588823224612753 35 23.04920642454167 24.539450803301126 24.90595280795544 27.505389964201765 36 22.321595129146896 26.042768886861456 25.907602399685224 25.72803358430642 37 22.573645537901598 33.12584613225407 21.882744303070872 22.417764026773458 38 26.577603807391615 27.056106110652518 22.059658285174365 24.306631796781502 39 22.79122032750003 30.98954671541244 23.14566848611129 23.073564470976237 40 21.496938800989444 27.965045706548576 25.19192400848324 25.346091483978743 41 22.18026436299439 31.295730212547458 22.72197780333857 23.80202762111958 42 21.819290980509596 31.173500848008075 23.00345826043782 24.00374991104451 43 23.57342921492224 26.200764306566484 22.77397588673654 27.45183059177474 44 22.41383651287785 27.187484664147497 22.67504208691348 27.723636736061174 45 22.714718611393618 25.662452949790065 29.405099201338075 22.21772923747824 46 21.48604553936514 28.50482823318276 26.197983052748576 23.811143174703524 47 22.134859825528256 30.393859809260775 26.349824341332717 21.121456023878252 48 24.473635027129482 26.86885870740921 22.7435877436834 25.913918521777905 49 19.733006134969326 27.904785276073618 22.951874573960463 29.410334014996593 50 19.865100658928288 27.600380920801225 26.337721470871895 26.196796949398593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 80976.0 1 68062.5 2 43908.5 3 30874.0 4 25992.0 5 17236.5 6 8604.0 7 5616.0 8 5542.0 9 5433.5 10 5078.5 11 4615.0 12 4319.0 13 4253.0 14 4663.0 15 5254.5 16 5601.5 17 5705.0 18 6017.5 19 6792.0 20 7433.5 21 8603.5 22 10123.0 23 12262.0 24 14856.5 25 17233.0 26 20496.0 27 24120.0 28 25808.0 29 27241.5 30 32010.5 31 43159.0 32 59723.5 33 66620.0 34 69604.0 35 79937.5 36 84526.5 37 82552.5 38 83307.5 39 90237.5 40 101365.5 41 117170.5 42 130394.5 43 133527.5 44 135409.0 45 143530.0 46 159169.5 47 171290.0 48 190989.0 49 225707.5 50 265703.0 51 296714.0 52 289992.5 53 261163.5 54 250073.0 55 245769.5 56 235211.5 57 231088.0 58 225249.5 59 199710.5 60 168711.0 61 149648.0 62 133013.0 63 112055.0 64 91892.5 65 78139.0 66 60081.5 67 43155.5 68 33243.0 69 25127.0 70 19194.5 71 14437.5 72 11079.0 73 7832.0 74 4911.0 75 3167.5 76 2117.5 77 1257.5 78 707.5 79 516.5 80 413.5 81 349.0 82 262.0 83 204.0 84 120.0 85 56.5 86 32.0 87 22.0 88 15.0 89 13.0 90 10.5 91 5.0 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 16805.0 25 19295.0 26 21109.0 27 24423.0 28 24021.0 29 20701.0 30 21340.0 31 20948.0 32 18766.0 33 16508.0 34 19424.0 35 18850.0 36 15126.0 37 15551.0 38 17663.0 39 20314.0 40 25257.0 41 26766.0 42 32477.0 43 55149.0 44 130038.0 45 251947.0 46 301627.0 47 300715.0 48 442163.0 49 422284.0 50 494591.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.457109789986038 #Duplication Level Percentage of deduplicated Percentage of total 1 72.19008102175547 8.2708968401424 2 10.884046009549916 2.4939942018134564 3 3.9027690157952795 1.3414335929676682 4 1.9066096107837531 0.8737694254956805 5 1.0780214791490794 0.6175505221287074 6 0.7722214662031068 0.5308455672283793 7 0.5268810713675426 0.4225573996646384 8 0.42330553458079234 0.3879886387520696 9 0.34978415855832007 0.36067639566605025 >10 3.932391585368771 11.016408200988812 >50 2.133931711022502 17.87654249555245 >100 1.7714159425609777 34.556379790984344 >500 0.08015783515053407 6.418504581963664 >1k 0.04441177352934997 9.11079742910863 >5k 0.002166427977041462 1.3891488115906019 >10k+ 0.001805356647534551 4.332506105952464 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 37779 1.3522161827838064 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 24679 0.8833305057021509 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 17583 0.629344798482958 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 14284 0.5112643520178907 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 10405 0.3724240816820325 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 6122 0.2191235202361752 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 6117 0.2189445562372891 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 5546 0.19850686756449323 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5461 0.1954644795834291 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 5304 0.18984501001840465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 5030 0.18003778287944483 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 4218 0.15097402946033764 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4160 0.14889804707325854 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 4083 0.14614200149041218 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4005 0.1433501631077886 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 3860 0.13816020714009086 No Hit CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC 3644 0.13042896238821014 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 3566 0.12763712400558655 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 3563 0.12752974560625488 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 3186 0.1140358600902408 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3158 0.11303366169647848 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 2985 0.10684150733501845 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2893 0.1035485697555137 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2884 0.10322643455751868 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 2868 0.10265374976108307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.045743198115294335 0.0 0.0 0.0 0.0 2 0.08669016106044043 0.0 0.0 0.0 0.0 3 0.11496647288444867 0.0 0.0 0.0 0.0 4 0.143099613509348 0.0 0.0 0.0 0.0 5 0.17080324053692064 0.0 0.0 0.0 0.0 6 0.21565161865778432 0.0 0.0 0.0 0.0 7 0.24561019207132218 0.0 0.0 0.0 0.0 8 0.2808660998518894 0.0 0.0 0.0 0.0 9 0.3142607820440409 0.0 0.0 0.0 0.0 10 0.3414275170749551 0.0 0.0 0.0 0.0 11 0.3699185856976267 0.0 0.0 0.0 0.0 12 0.4018815558986892 0.0 0.0 0.0 0.0 13 0.4296925613255935 0.0 0.0 0.0 0.0 14 0.4541390435734386 0.0 0.0 0.0 0.0 15 0.48288066179455075 0.0 0.0 0.0 0.0 16 0.5096536760279156 0.0 0.0 0.0 0.0 17 0.5450169622078145 0.0 0.0 0.0 0.0 18 0.580487626787045 0.0 0.0 0.0 0.0 19 0.603967703440905 0.0 0.0 0.0 0.0 20 0.6291300416842946 0.0 0.0 0.0 0.0 21 0.6528964607363724 0.0 0.0 0.0 0.0 22 0.6729404286116187 0.0 0.0 0.0 0.0 23 0.6989260012498846 0.0 0.0 0.0 0.0 24 0.7221197355055268 0.0 0.0 0.0 0.0 25 0.7450271273629512 0.0 0.0 0.0 0.0 26 0.7734466103860683 0.0 0.0 0.0 0.0 27 0.7959244886461659 0.0 0.0 0.0 0.0 28 0.8216595116859912 0.0 0.0 0.0 0.0 29 0.8499358235099994 0.0 0.0 0.0 0.0 30 0.8791427481282156 0.0 0.0 0.0 0.0 31 0.9051283207664813 0.0 0.0 0.0 0.0 32 0.9321876773980639 0.0 0.0 0.0 0.0 33 0.9580300788372208 0.0 0.0 0.0 0.0 34 0.9838366874766005 0.0 0.0 0.0 0.0 35 1.0054555385420447 0.0 0.0 0.0 0.0 36 1.0250699928199645 0.0 0.0 0.0 0.0 37 1.0451139606952107 0.0 0.0 0.0 0.0 38 1.0573193054192447 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATACGC 40 2.4556357E-10 166.7462 44 CTATCAT 2800 0.0 149.67456 44 CGAGTTA 15 0.0066393395 148.21883 44 CGTTCTA 25 1.7141149E-4 133.39696 44 CAATCGT 35 4.9112714E-11 120.63241 43 AACTACC 830 0.0 100.44952 44 AATCGCA 325 0.0 82.09044 44 CTATCGA 15 0.0012224421 81.40362 42 CTTAACC 220 0.0 80.84664 44 AAGTGTA 55 3.880651E-5 80.84664 44 TCGTGCG 245 0.0 78.78035 43 AGAGCCA 625 0.0 78.259544 44 ACGAACA 285 0.0 78.00992 44 CCTATCA 2985 0.0 77.79476 43 GTTCGGC 80 0.0 75.395256 43 TGCGTAG 75 2.0226726E-6 74.10942 44 ACCTTAG 4855 0.0 72.60097 42 TGTCGCA 25 0.0019485105 72.37945 43 ACTTCTA 1805 0.0 70.20892 44 ACAGCGT 95 1.0021904E-7 70.20892 44 >>END_MODULE