FastQCFastQC Report
Tue 24 May 2016
ERR841010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836243
Sequences flagged as poor quality0
Sequence length24-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC175082.0936498123153195No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA117111.400430257712172No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT81270.9718467000620633No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA66430.7943863207225651No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT65060.7780035228994443No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC51590.6169259413830669No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA51550.6164476115196181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA51070.6107076531582327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA44270.5293915763719398No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG44240.5290328289743531No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT42310.5059534130629494No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA41470.495908485930525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA40950.4896901977056908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34790.41602739873457834No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA32030.38302263815661236No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC30660.36663984033349156No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA30050.35934530991589764No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27060.32359015262310115No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT26790.3203614260448219No Hit
AAAAAAAAAAAAAAAAAAAAAAAA26610.3182089416593024No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT26600.31808935919344017No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA24280.2903462271134108No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC20310.24287198816611918No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC19900.23796910706576918No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19420.23222914870438377No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT19100.22840250979679352No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA18700.22361921116230568No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC17890.21393303142746783No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG17860.21357428402988127No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG16370.19575649661641414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16280.19468025442365436No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG16270.19456067195779217No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG16020.19157111031123727No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT15730.1881032188012336No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG15520.1855919870181275No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA14660.17530789495397867No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG14240.17028543138776647No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT13980.1671762872753494No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC13660.16334964836775914No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13400.16024050425534206No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG12970.15509845822326765No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA12650.1512718193156774No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA12440.14876058753257126No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11640.13919399026359563No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT11630.13907440779773345No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC11300.13512818642428098No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG11210.1340519442315212No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT11150.13333444943634803No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10930.13070363518737974No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC10510.12568117162116754No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA10410.12448534696254557No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA10310.12328952230392363No Hit
TAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACC10080.12053912558909312No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT9580.11456000229598333No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9300.11121169325184187No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA8890.10630881215149186No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA8690.10391716283424794No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG8500.10164509598286622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT203.6769416E-7206.222144
CACGCTC203.6769416E-7206.222144
CAAGGCG254.5056368E-9206.222144
CAGGCGG153.016371E-5206.222144
TTCGCAA153.016371E-5206.222144
CCCTCCG251.1175962E-6164.9776844
AGATACG251.1175962E-6164.9776844
TGTCCCA251.1175962E-6164.9776844
TTATGCC150.00830776137.4814144
GATAGCC150.00830776137.4814144
CTATCAT13900.0130.5578944
GAACACA10800.0129.8435544
TAAGTAT252.3094933E-4123.7332644
TATTTCG301.9990694E-9123.3601643
ACTAGGG152.3391783E-4123.3601643
GTAAGTA301.9990694E-9123.3601643
AGCCCCG204.754729E-6123.3601543
CATTGGC204.754729E-6123.3601543
TATTAGT204.754729E-6123.3601543
TCCGGGC1150.0123.3601543
Produced by FastQC (version 0.11.5)