##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841010.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	836243
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.57560063283041	39.0	37.0	39.0	32.0	39.0
2	37.36784283994007	39.0	37.0	39.0	35.0	39.0
3	37.72547214147084	39.0	37.0	39.0	35.0	39.0
4	38.163793299316104	39.0	37.0	40.0	35.0	41.0
5	38.84480826745336	40.0	38.0	41.0	35.0	41.0
6	38.80927792519638	40.0	38.0	41.0	35.0	41.0
7	38.884971234437835	40.0	38.0	41.0	35.0	41.0
8	38.8566588898203	40.0	38.0	41.0	35.0	41.0
9	38.909865912181026	40.0	38.0	41.0	35.0	41.0
10	38.91490631311712	40.0	38.0	41.0	35.0	41.0
11	38.79087657534951	40.0	38.0	41.0	35.0	41.0
12	38.81508006644002	40.0	38.0	41.0	35.0	41.0
13	38.68452112603633	40.0	38.0	41.0	35.0	41.0
14	38.62607041254755	40.0	38.0	41.0	35.0	41.0
15	38.4871813575719	40.0	38.0	41.0	35.0	41.0
16	38.46721826072087	40.0	37.0	41.0	35.0	41.0
17	38.416280913562204	40.0	37.0	41.0	35.0	41.0
18	38.37890421803232	40.0	37.0	41.0	35.0	41.0
19	38.406949893750976	40.0	37.0	41.0	35.0	41.0
20	38.31410965472955	40.0	37.0	41.0	35.0	41.0
21	38.27379123053945	40.0	37.0	41.0	35.0	41.0
22	38.20667317992498	40.0	37.0	41.0	34.0	41.0
23	38.12777506059842	40.0	36.0	41.0	34.0	41.0
24	37.924142862780315	39.0	36.0	41.0	34.0	41.0
25	37.84170719768123	39.0	36.0	41.0	33.0	41.0
26	37.76052420381157	39.0	36.0	41.0	33.0	41.0
27	37.64230229904603	39.0	36.0	41.0	33.0	41.0
28	37.49534496269846	39.0	35.0	41.0	33.0	41.0
29	37.55591316444556	39.0	36.0	41.0	33.0	41.0
30	37.56443465473064	39.0	36.0	41.0	33.0	41.0
31	37.632255133350604	39.0	36.0	41.0	33.0	41.0
32	37.66521851652848	39.0	36.0	41.0	33.0	41.0
33	37.58360273345142	39.0	36.0	41.0	33.0	41.0
34	37.512637298216255	39.0	35.0	40.0	33.0	41.0
35	37.361064688761616	38.0	35.0	40.0	33.0	41.0
36	37.37645869084733	38.0	35.0	40.0	33.0	41.0
37	37.3138353303705	38.0	35.0	40.0	33.0	41.0
38	37.158478141676774	38.0	35.0	40.0	32.0	41.0
39	37.16459406387479	38.0	35.0	40.0	32.0	41.0
40	36.93949427303483	38.0	35.0	40.0	31.0	41.0
41	36.85421253032617	38.0	35.0	40.0	31.0	41.0
42	36.740057699774134	38.0	35.0	40.0	31.0	41.0
43	36.7000631630324	38.0	35.0	40.0	31.0	41.0
44	36.44460913836438	38.0	34.0	40.0	30.0	41.0
45	36.209320963254044	38.0	34.0	40.0	29.0	41.0
46	35.82562628733487	37.0	34.0	40.0	28.0	41.0
47	36.03177915905238	37.0	34.0	40.0	29.0	41.0
48	36.863962150290845	38.0	35.0	40.0	31.0	41.0
49	37.814889496468446	39.0	36.0	40.0	33.0	41.0
50	37.19546049371736	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	28.0
22	64.0
23	118.0
24	222.0
25	364.0
26	505.0
27	937.0
28	1658.0
29	3042.0
30	5938.0
31	11559.0
32	20541.0
33	37108.0
34	49473.0
35	47726.0
36	83728.0
37	136422.0
38	135795.0
39	134559.0
40	166448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.07358626619296	17.088334371707745	34.83807936209929
2	21.18654505927105	32.987301537950096	29.57812501868476	16.248028384094095
3	22.379978068575763	33.50329987814546	19.232208819685187	24.884513233593587
4	19.71376740971225	35.33231369350775	22.956006806633958	21.997912090146045
5	15.305240223236547	36.52706210993694	23.19636756301697	24.97133010380954
6	17.474466153976774	35.740329067029556	21.223496041222468	25.5617087377712
7	19.313405314005617	35.77189883801718	19.424258259859872	25.49043758811733
8	17.32020477301454	31.379515284432873	27.178224511296357	24.122055431256225
9	18.48673172750026	38.59189254797947	23.77167880627999	19.149696918240274
10	26.130203780480077	28.68316984417209	21.11407808495856	24.072548290389275
11	17.01168201109008	35.279936573460105	20.71921678268159	26.989164632768226
12	17.0236402576763	34.23825371333452	22.569277112035614	26.16882891695356
13	16.507044005151613	34.658227333442554	21.96036319586532	26.874365465540517
14	14.91205307548165	33.80428894472061	21.89949572074146	29.38416225905628
15	15.093100928797012	41.27077894822438	23.97508858071159	19.661031542267022
16	14.861469692421938	45.048269462345274	19.223240134745524	20.867020710487264
17	17.997878607055604	42.881674345854016	17.77808603480089	21.34236101228949
18	17.44469011997709	35.79569574872375	21.234138880684203	25.525475250614953
19	19.06527169734156	29.94918941025515	23.317624183401236	27.66791470900205
20	16.291317236736212	32.50311213367406	26.045658977115504	25.15991165247422
21	14.936687063449261	41.67867473928033	21.951992423254964	21.432645774015448
22	19.2666485698535	36.93077251468772	18.037221238324268	25.765357677134514
23	22.867515781895932	30.92940688292757	25.492590072502846	20.71048726267365
24	17.047437168382874	34.39263467676262	23.541841306892852	25.01808684796166
25	16.61417930422495	33.70424134862781	23.353701323597853	26.327878023549385
26	19.245771990096586	41.08844995500109	23.565302004186908	16.100476050715415
27	20.482186028286474	36.09839887425852	20.077160607920113	23.34225448953489
28	21.00397324837739	27.88722923730879	22.762376249998436	28.34642126431538
29	20.427964000705074	34.71139567648316	24.307386398941883	20.553253923869885
30	22.14219202537905	32.2691514844046	28.02162870298422	17.567027787232128
31	19.656114652749483	35.39769554118071	22.748036549534408	22.198153256535395
32	17.27769669246362	33.50179518944405	24.707797283503304	24.512710834589026
33	15.820472290840925	29.131882941346234	32.61886039116632	22.42878437664652
34	17.941499368468175	33.84877344262121	26.327549176876875	21.882178012033744
35	16.10888313639229	32.311922421771264	23.43428517778344	28.14490926405301
36	15.730165652463318	32.772697384854055	26.3759928818992	25.121144080783424
37	13.65625598669578	40.51958744957589	19.290441531126742	26.53371503260159
38	21.91780072712953	31.478774736145503	21.777242404313007	24.826182132411954
39	17.41289082334874	34.0656361677329	28.12964856344339	20.391824445474974
40	14.487265238834013	33.0666670367153	28.46594488680675	23.98012283764394
41	16.31197209991913	35.39022486297061	25.484516802947255	22.813286234162998
42	17.375680508755572	36.23494803902953	22.640076404023112	23.749295048191783
43	16.092074474957293	27.906719685333258	24.058942593416692	31.94226324629276
44	17.902798689999823	28.900121274613767	22.326246287063455	30.87083374832295
45	15.043287526836716	28.98845924306896	32.187938397625196	23.78031483246913
46	18.63405360833279	32.55486591016516	26.94631231390787	21.864768167594182
47	17.96217816519394	34.166767961663105	23.65708587686786	24.21396799627509
48	16.681423370662024	34.289798583356415	24.4037137430256	24.625064302955955
49	14.629376471481734	30.553656869446343	20.56390977443609	34.25305688463583
50	18.967970625976697	32.217408428515164	28.53549149398417	20.279129451523968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55548.0
1	52114.0
2	33602.0
3	17537.5
4	14871.5
5	10423.0
6	5507.5
7	3258.5
8	2774.5
9	2040.5
10	1483.0
11	1356.5
12	1537.0
13	1712.0
14	1905.5
15	2140.0
16	2215.0
17	2097.0
18	1947.0
19	2036.5
20	2340.5
21	2781.5
22	3477.5
23	4841.5
24	6461.0
25	7595.0
26	8727.0
27	9787.5
28	10207.0
29	10899.0
30	12819.5
31	17009.0
32	25452.5
33	32340.5
34	39719.5
35	48537.0
36	48744.5
37	45594.0
38	45935.0
39	49592.5
40	54161.0
41	60144.0
42	64953.5
43	62721.5
44	59112.5
45	58386.0
46	58678.5
47	56679.5
48	70684.0
49	89682.0
50	83170.0
51	73495.0
52	70309.5
53	59964.0
54	46531.5
55	38831.5
56	34295.0
57	29105.5
58	23989.0
59	19654.0
60	16383.0
61	13561.5
62	11200.0
63	8549.5
64	5881.5
65	4614.5
66	4043.0
67	3076.5
68	2176.0
69	1632.5
70	1288.5
71	1005.5
72	770.5
73	628.5
74	541.0
75	466.5
76	414.5
77	302.0
78	142.0
79	60.0
80	52.5
81	44.0
82	36.0
83	118.0
84	200.0
85	194.0
86	188.0
87	95.5
88	5.0
89	5.0
90	4.0
91	2.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	6837.0
25	8275.0
26	9577.0
27	11453.0
28	11530.0
29	9976.0
30	10432.0
31	6962.0
32	5819.0
33	4826.0
34	4986.0
35	4923.0
36	4640.0
37	4638.0
38	4666.0
39	6077.0
40	8370.0
41	6534.0
42	9112.0
43	13862.0
44	37643.0
45	65419.0
46	94305.0
47	81045.0
48	140996.0
49	105922.0
50	157418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.960263221472978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.35	8.17483991187678
2	11.652999999999999	2.7874589463964923
3	4.376	1.5701433557149727
4	2.247	1.0749884583459914
5	1.465	0.8760892809728957
6	1.032	0.7405794986736068
7	0.751	0.6287510375528345
8	0.6649999999999999	0.6362860033823624
9	0.536	0.5769630978038565
>10	5.321	15.095287417085476
>50	2.094	17.974481582390663
>100	1.413	27.528459446335496
>500	0.044000000000000004	3.464649049996292
>1k	0.045	10.987295808406152
>5k	0.006	4.389057794383939
>10k+	0.002	3.4946693106821893
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	17508	2.0936498123153195	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	11711	1.400430257712172	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8127	0.9718467000620633	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	6643	0.7943863207225651	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	6506	0.7780035228994443	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5159	0.6169259413830669	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5155	0.6164476115196181	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5107	0.6107076531582327	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	4427	0.5293915763719398	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	4424	0.5290328289743531	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	4231	0.5059534130629494	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	4147	0.495908485930525	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4095	0.4896901977056908	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3479	0.41602739873457834	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3203	0.38302263815661236	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	3066	0.36663984033349156	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3005	0.35934530991589764	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2706	0.32359015262310115	No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	2679	0.3203614260448219	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2661	0.3182089416593024	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	2660	0.31808935919344017	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	2428	0.2903462271134108	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2031	0.24287198816611918	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1990	0.23796910706576918	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1942	0.23222914870438377	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	1910	0.22840250979679352	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	1870	0.22361921116230568	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1789	0.21393303142746783	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1786	0.21357428402988127	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG	1637	0.19575649661641414	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1628	0.19468025442365436	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	1627	0.19456067195779217	No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1602	0.19157111031123727	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1573	0.1881032188012336	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1552	0.1855919870181275	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1466	0.17530789495397867	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	1424	0.17028543138776647	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	1398	0.1671762872753494	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1366	0.16334964836775914	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1340	0.16024050425534206	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1297	0.15509845822326765	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1265	0.1512718193156774	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	1244	0.14876058753257126	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1164	0.13919399026359563	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1163	0.13907440779773345	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1130	0.13512818642428098	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	1121	0.1340519442315212	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	1115	0.13333444943634803	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1093	0.13070363518737974	No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	1051	0.12568117162116754	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	1041	0.12448534696254557	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	1031	0.12328952230392363	No Hit
TAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACC	1008	0.12053912558909312	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	958	0.11456000229598333	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	930	0.11121169325184187	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	889	0.10630881215149186	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	869	0.10391716283424794	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	850	0.10164509598286622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12340910476978581	0.0	0.0	0.0	0.0
2	0.2367732824071472	0.0	0.0	0.0	0.0
3	0.31880685398861336	0.0	0.0	0.0	0.0
4	0.3715427214338416	0.0	0.0	0.0	0.0
5	0.41662531106388934	0.0	0.0	0.0	0.0
6	0.47844944591464444	0.0	0.0	0.0	0.0
7	0.529511158837802	0.0	0.0	0.0	0.0
8	0.5890632268371753	0.0	0.0	0.0	0.0
9	0.6458648981217182	0.0	0.0	0.0	0.0
10	0.6953720389886672	0.0	0.0	0.0	0.0
11	0.7536086998635564	0.0	0.0	0.0	0.0
12	0.8131607678629298	0.0	0.0	0.0	0.0
13	0.8761807273723069	0.0	0.0	0.0	0.0
14	0.926405363034429	0.0	0.0	0.0	0.0
15	0.9744775143110316	0.0	0.0	0.0	0.0
16	1.015733465033489	0.0	0.0	0.0	0.0
17	1.0627293741173318	0.0	0.0	0.0	0.0
18	1.1183352207432529	0.0	0.0	0.0	0.0
19	1.147752507345353	0.0	0.0	0.0	0.0
20	1.1848230717626336	0.0	0.0	0.0	0.0
21	1.2122074564450764	0.0	0.0	0.0	0.0
22	1.24018975345683	0.0	0.0	0.0	0.0
23	1.264584576492718	0.0	0.0	0.0	0.0
24	1.2905339715848145	0.0	0.0	0.0	0.0
25	1.3217449951748474	0.0	0.0	0.0	0.0
26	1.353793096025916	0.0	0.0	0.0	0.0
27	1.378307501527666	0.0	0.0	0.0	0.0
28	1.40521355634666	0.0	0.0	0.0	0.0
29	1.4425232856956651	0.0	0.0	0.0	0.0
30	1.475528046273631	0.0	0.0	0.0	0.0
31	1.5082936419198725	0.0	0.0	0.0	0.0
32	1.5350801142730044	0.0	0.0	0.0	0.0
33	1.5621057515578605	0.0	0.0	0.0	0.0
34	1.5890118063768546	0.0	0.0	0.0	0.0
35	1.6162766085934352	0.0	0.0	0.0	0.0
36	1.6379210349144926	0.0	0.0	0.0	0.0
37	1.6586088015086524	0.0	0.0	0.0	0.0
38	1.6727195324803914	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	3.6769416E-7	206.2221	44
CACGCTC	20	3.6769416E-7	206.2221	44
CAAGGCG	25	4.5056368E-9	206.2221	44
CAGGCGG	15	3.016371E-5	206.2221	44
TTCGCAA	15	3.016371E-5	206.2221	44
CCCTCCG	25	1.1175962E-6	164.97768	44
AGATACG	25	1.1175962E-6	164.97768	44
TGTCCCA	25	1.1175962E-6	164.97768	44
TTATGCC	15	0.00830776	137.48141	44
GATAGCC	15	0.00830776	137.48141	44
CTATCAT	1390	0.0	130.55789	44
GAACACA	1080	0.0	129.84355	44
TAAGTAT	25	2.3094933E-4	123.73326	44
TATTTCG	30	1.9990694E-9	123.36016	43
ACTAGGG	15	2.3391783E-4	123.36016	43
GTAAGTA	30	1.9990694E-9	123.36016	43
AGCCCCG	20	4.754729E-6	123.36015	43
CATTGGC	20	4.754729E-6	123.36015	43
TATTAGT	20	4.754729E-6	123.36015	43
TCCGGGC	115	0.0	123.36015	43
>>END_MODULE