FastQCFastQC Report
Tue 24 May 2016
ERR841009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173239
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCACTGCA42870.36539869540647724No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG28960.24683802703455987No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT28620.2439400667724138No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCACTGC26960.22979120196311237No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC25930.22101208705131692No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA25340.2159832736552399No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG25140.21427859114809514No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCAC22820.19450427406521603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA21860.18632179803092125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA21270.18129298463484422No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA21080.1796735362530567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA19590.1669736515748283No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG18060.15393283039517097No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA17900.15256908438945518No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT17640.150352997130167No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG17440.14864831462302225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16650.14191481871980047No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC14660.12495322777371022No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC14440.123078077015851No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCACTG14270.12162909688477794No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA14140.12052105325513388No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC13670.11651504936334371No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA13480.11489560098155618No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG13110.1117419383433384No Hit
AAAAAAAAAAAAAAAAAAAAAAAA12840.10944061695869298No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCA12530.10679835907261863No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA11890.10134337504975544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG151.686765E-5238.5883344
ACGACAC350.0238.5883344
TCAAGCG401.4915713E-10178.9412444
TAACGGT150.0053734626159.0588844
CTATCAT4200.0150.5378744
TCGCGTA257.8483936E-8127.8734943
CAACGGT152.0279837E-4127.87347443
CCGTCCA152.0279837E-4127.87347443
TGTAACA750.0127.24711644
AAGGGGG950.0125.5728144
TTATGGT705.4569682E-11119.2941744
TTCGGTC302.671857E-4119.2941744
AACGACA850.0105.3075843
CAGGACG707.357812E-9102.25214444
AATCGCA354.9334083E-4102.25214444
TCACTAC903.965397E-1092.7843544
CCAGAAT4750.090.4124244
CTACAGA2000.089.4706344
GTTACCA408.388063E-489.4706244
GTTCGGC602.3646862E-1185.24898543
Produced by FastQC (version 0.11.5)