##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841009.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173239
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.61191198042343	38.0	35.0	39.0	30.0	39.0
2	36.78451619831935	39.0	35.0	39.0	33.0	39.0
3	36.96522959090177	39.0	35.0	39.0	33.0	39.0
4	37.305041001876006	39.0	35.0	39.0	33.0	41.0
5	38.03341859587007	40.0	35.0	41.0	34.0	41.0
6	38.027892015181905	40.0	35.0	41.0	34.0	41.0
7	37.94210557269235	39.0	35.0	41.0	34.0	41.0
8	37.97436839382257	39.0	35.0	41.0	34.0	41.0
9	38.00840152773646	40.0	35.0	41.0	34.0	41.0
10	37.67587337277401	39.0	35.0	41.0	33.0	41.0
11	37.7708804429447	39.0	35.0	41.0	34.0	41.0
12	37.76041624937459	39.0	35.0	41.0	34.0	41.0
13	37.6527527639296	39.0	35.0	40.0	33.0	41.0
14	37.554148813668824	39.0	35.0	40.0	33.0	41.0
15	37.461660411902436	39.0	35.0	40.0	33.0	41.0
16	37.411615195198934	38.0	35.0	40.0	33.0	41.0
17	37.42707240383247	38.0	35.0	40.0	33.0	41.0
18	37.409185170285	38.0	35.0	40.0	33.0	41.0
19	37.419265810290995	38.0	35.0	40.0	33.0	41.0
20	37.3343393801263	38.0	35.0	40.0	33.0	41.0
21	37.17052535757846	38.0	35.0	40.0	33.0	41.0
22	36.97879119258736	38.0	35.0	40.0	33.0	41.0
23	36.713537480428116	38.0	35.0	40.0	32.0	41.0
24	36.58327160962089	37.0	35.0	40.0	32.0	41.0
25	36.51938093623523	37.0	35.0	40.0	32.0	41.0
26	36.32234576447175	37.0	35.0	40.0	32.0	41.0
27	36.1490471580453	36.0	35.0	39.0	31.0	41.0
28	35.918554338404455	36.0	34.0	39.0	31.0	41.0
29	35.84420570312841	36.0	34.0	39.0	31.0	41.0
30	35.786920625789314	36.0	34.0	39.0	31.0	41.0
31	35.60726686417668	36.0	34.0	39.0	31.0	40.0
32	35.569996860674536	35.0	34.0	39.0	31.0	40.0
33	35.31853337923521	35.0	34.0	38.0	31.0	40.0
34	35.2354100865163	35.0	34.0	38.0	31.0	40.0
35	35.05992847575524	35.0	34.0	38.0	30.0	40.0
36	35.03762568029659	35.0	34.0	38.0	30.0	40.0
37	34.91036724751012	35.0	34.0	37.0	30.0	40.0
38	34.79583334142821	35.0	34.0	37.0	30.0	40.0
39	34.63845181451296	35.0	33.0	37.0	30.0	40.0
40	34.42057611370963	35.0	33.0	37.0	29.0	40.0
41	34.24236547059291	35.0	33.0	36.0	29.0	39.0
42	34.11957215527746	35.0	33.0	36.0	29.0	39.0
43	34.0031806405152	35.0	33.0	36.0	29.0	39.0
44	33.760999018153534	35.0	32.0	36.0	29.0	39.0
45	33.61297642145418	35.0	32.0	36.0	28.0	39.0
46	33.46155256809942	35.0	32.0	35.0	27.0	39.0
47	33.41863230644394	35.0	32.0	35.0	28.0	38.0
48	34.00472268251356	35.0	33.0	36.0	29.0	39.0
49	34.36729165500643	35.0	34.0	36.0	30.0	39.0
50	34.361944852408826	35.0	33.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	17.0
22	32.0
23	76.0
24	230.0
25	578.0
26	1141.0
27	2516.0
28	5319.0
29	10233.0
30	19177.0
31	33914.0
32	58740.0
33	98885.0
34	157044.0
35	115671.0
36	164557.0
37	194843.0
38	158153.0
39	100133.0
40	51978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.11611700599792	18.019431675898943	28.86445131810313
2	27.877184444090247	27.390838524801854	23.348524895609504	21.383452135498395
3	31.239500221182553	20.995722099248322	23.785775958692135	23.97900172087699
4	20.54304365947603	20.995892567499038	27.064221356432917	31.396842416592012
5	17.252239313558448	32.30313687151552	27.479908185800166	22.964715629125866
6	26.380302734566445	27.398935766710792	24.184927367740077	22.03583413098269
7	24.640674236025227	24.2558421600373	28.59178735108533	22.511696252852147
8	28.113282971329795	23.308976261443746	24.955614329220218	23.622126438006237
9	27.88800917801062	24.24748921575229	26.052577522567866	21.811924083669226
10	24.77014487244287	24.333149511736313	28.87834448053636	22.018361135284454
11	26.322940168201026	27.725467700954365	24.704599830043154	21.246992300801455
12	24.232573243814773	24.361788177856344	30.440771232459884	20.964867345869003
13	23.893511893143682	26.51582499388445	22.804901644081042	26.785761468890822
14	22.068990205746655	29.10165788897232	23.882942861599386	24.946409043681637
15	21.61597082947294	31.926913442188678	24.096369111493907	22.36074661684448
16	23.11268207074603	30.016134819930123	21.99014863979121	24.881034469532636
17	27.435756908865116	29.0259699856551	20.838976542716363	22.699296562763426
18	22.09941878849919	27.989523021311086	22.279944666005818	27.631113524183903
19	25.085681604515365	29.394437109574433	23.10305061458066	22.416830671329542
20	28.96323766939217	27.005409809936427	22.227269976535045	21.80408254413636
21	24.152623634229684	32.213300103388995	21.69890363344553	21.935172628935792
22	27.931308113692094	29.148451423793446	21.68807889952516	21.232161562989297
23	25.770282099384694	27.211335456799514	26.254752867915233	20.763629575900563
24	25.88458106148875	28.263209797833177	23.664061627682	22.188147512996075
25	23.255235045944797	27.40318544873968	25.975184229839872	23.36639527547565
26	24.653543307086615	31.550921519425458	24.6305269533616	19.16500822012633
27	28.014861717993668	27.428504147151937	23.173639638225314	21.382994496629077
28	27.961085260244307	26.273533620970152	24.828030745924632	20.937350372860912
29	28.23817281834807	29.432136726860712	22.783742351781104	19.54594810301012
30	28.495525903021534	27.22462169049406	22.296238702399517	21.98361370408489
31	25.56004644154407	27.084046092140607	26.331554772632977	21.02435269368235
32	26.955148034800615	26.80864617966121	24.7063078411538	21.52989794438437
33	22.365010889324193	24.818843773691356	30.84040342927878	21.97574190770567
34	24.282416465244637	25.385447812191664	25.407914715634533	24.924221006929166
35	22.892575722940844	24.619189127407992	25.971178714263853	26.51705643538731
36	24.293344747476382	25.667427079927286	24.842348504419963	25.19687966817637
37	25.445926912242356	27.621492234040918	24.801521635392454	22.13105921832428
38	25.59269052258567	25.260768287778617	25.95472569060612	23.191815499029587
39	26.975667781598904	27.141828017776103	25.125626167930783	20.75687803269421
40	23.372686773292614	27.271328250580574	26.59390367524923	22.762081300877586
41	22.509513956650324	28.042531682204203	25.435501554088614	24.012452807056857
42	23.564850114881214	31.390719308086272	23.3397704818903	21.70466009514221
43	24.81551457258979	26.50040559892061	25.609433072039334	23.074646756450264
44	22.514037004135655	28.208002686263928	24.664854571961694	24.613105737638723
45	22.20077767357894	27.877069061904315	25.56910896388433	24.353044300632412
46	22.597428245695255	28.230049796160195	24.768684460597168	24.403837497547382
47	23.338960550410228	25.340975156302946	27.917214620815727	23.402849672471092
48	23.780798783460895	27.037357390120103	24.351892155452475	24.829951670966523
49	20.258857110874796	28.940224995802318	23.104046566295406	27.696871327027484
50	23.41859214364024	29.617007632956167	23.63320439360404	23.331195829799558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23006.0
1	22127.0
2	17293.5
3	12744.0
4	11214.5
5	7778.0
6	4292.0
7	3649.5
8	4024.0
9	3798.0
10	3643.5
11	3972.5
12	4249.0
13	4266.5
14	4327.0
15	4514.5
16	4659.5
17	4970.5
18	5343.0
19	5453.0
20	5590.5
21	6372.0
22	7422.5
23	8166.0
24	8482.0
25	8636.5
26	8634.0
27	8813.5
28	9891.5
29	11524.0
30	13487.5
31	16192.0
32	19395.0
33	21047.0
34	22830.0
35	26366.0
36	31372.5
37	36809.0
38	40360.0
39	43257.0
40	48445.5
41	55981.5
42	63944.0
43	71413.0
44	76550.5
45	77697.0
46	79344.5
47	85977.0
48	96057.5
49	107809.5
50	120624.0
51	130116.5
52	130848.0
53	124419.0
54	115236.5
55	104750.5
56	94246.5
57	88616.0
58	83572.0
59	79298.0
60	76210.5
61	59939.5
62	40415.0
63	28773.0
64	21431.0
65	17801.0
66	13798.0
67	9278.5
68	6446.5
69	5045.5
70	4041.5
71	3187.0
72	2363.0
73	1701.0
74	1145.5
75	874.5
76	741.5
77	583.0
78	401.5
79	257.5
80	178.0
81	135.0
82	93.5
83	73.5
84	63.0
85	48.5
86	36.0
87	25.0
88	13.5
89	6.5
90	3.5
91	2.0
92	0.5
93	0.0
94	0.5
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	8254.0
25	9285.0
26	9130.0
27	12501.0
28	11422.0
29	10132.0
30	13499.0
31	9610.0
32	11745.0
33	9423.0
34	9576.0
35	9671.0
36	9583.0
37	9950.0
38	10562.0
39	11699.0
40	12080.0
41	12798.0
42	15847.0
43	25388.0
44	59223.0
45	102082.0
46	136477.0
47	108013.0
48	177949.0
49	163968.0
50	193372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.98174616313915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60018549976158	11.123324975645875
2	11.69123467953592	3.736926899640651
3	4.4302057854913075	2.1240727294257766
4	2.317886491254295	1.4817549415278157
5	1.4380944372936793	1.1491630127725005
6	1.010388282719659	0.9688661436381399
7	0.7433944979880437	0.8316519516143419
8	0.5950798600278194	0.7608332215808787
9	0.4606910272352031	0.6626382351217956
>10	4.707326551558556	17.59618245479361
>50	1.8064601713453532	20.453081919519136
>100	1.1478255809825921	29.792009145584565
>500	0.0275838418186106	3.0423217128110056
>1k	0.023643292987380517	6.277172656323914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCACTGCA	4287	0.36539869540647724	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	2896	0.24683802703455987	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	2862	0.2439400667724138	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCACTGC	2696	0.22979120196311237	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	2593	0.22101208705131692	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	2534	0.2159832736552399	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	2514	0.21427859114809514	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCAC	2282	0.19450427406521603	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	2186	0.18632179803092125	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2127	0.18129298463484422	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	2108	0.1796735362530567	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	1959	0.1669736515748283	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	1806	0.15393283039517097	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1790	0.15256908438945518	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1764	0.150352997130167	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1744	0.14864831462302225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1665	0.14191481871980047	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1466	0.12495322777371022	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	1444	0.123078077015851	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCACTG	1427	0.12162909688477794	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA	1414	0.12052105325513388	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC	1367	0.11651504936334371	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	1348	0.11489560098155618	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	1311	0.1117419383433384	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	1284	0.10944061695869298	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCA	1253	0.10679835907261863	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1189	0.10134337504975544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06886917328864792	0.0	0.0	0.0	0.0
2	0.2720673281403022	0.0	0.0	0.0	0.0
3	0.38508777836399916	0.0	0.0	0.0	0.0
4	0.44210940822799105	0.0	0.0	0.0	0.0
5	0.8530231265752332	0.0	0.0	0.0	0.0
6	0.9587134420182077	0.0	0.0	0.0	0.0
7	1.0728419358715489	0.0	0.0	0.0	0.0
8	1.186714727348818	0.0	0.0	0.0	0.0
9	1.2438215913381672	0.0	0.0	0.0	0.0
10	1.309707570239312	0.0	0.0	0.0	0.0
11	1.3784915094026025	0.0	0.0	0.0	0.0
12	1.438581567779455	0.0	0.0	0.0	0.0
13	1.5008877134155956	0.0	0.0	0.0	0.0
14	1.5521134227552953	0.0	0.0	0.0	0.0
15	1.6267785165682354	0.0	0.0	0.0	0.0
16	1.6947101144779537	0.0	0.0	0.0	0.0
17	1.8057701798184342	0.0	0.0	0.0	0.0
18	1.8777077816199428	0.0	0.0	0.0	0.0
19	2.0911340315144655	0.0	0.0	0.0	0.0
20	2.1450019987402396	0.0	0.0	0.0	0.0
21	2.222309350439254	0.0	0.0	0.0	0.0
22	2.262795559983942	0.0	0.0	0.0	0.0
23	2.3117199479389963	0.0	0.0	0.0	0.0
24	2.3540813082415433	0.0	0.0	0.0	0.0
25	2.40232382319374	0.0	0.0	0.0	0.0
26	2.5380165507624617	0.0	0.0	0.0	0.0
27	2.576798077800005	0.0	0.0	0.0	0.0
28	2.6255519975043446	0.0	0.0	0.0	0.0
29	2.668680464935107	0.0	0.0	0.0	0.0
30	2.7062687142176487	0.0	0.0	0.0	0.0
31	2.735759721591253	0.0	0.0	0.0	0.0
32	2.7661030702184295	0.0	0.0	0.0	0.0
33	2.7952531410906047	0.0	0.0	0.0	0.0
34	2.825426021467067	0.0	0.0	0.0	0.0
35	2.8494620448178076	0.0	0.0	0.0	0.0
36	2.870429639655688	0.0	0.0	0.0	0.0
37	2.8900334884878527	0.0	0.0	0.0	0.0
38	2.9015400954110797	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATG	15	1.686765E-5	238.58833	44
ACGACAC	35	0.0	238.58833	44
TCAAGCG	40	1.4915713E-10	178.94124	44
TAACGGT	15	0.0053734626	159.05888	44
CTATCAT	420	0.0	150.53787	44
TCGCGTA	25	7.8483936E-8	127.87349	43
CAACGGT	15	2.0279837E-4	127.873474	43
CCGTCCA	15	2.0279837E-4	127.873474	43
TGTAACA	75	0.0	127.247116	44
AAGGGGG	95	0.0	125.57281	44
TTATGGT	70	5.4569682E-11	119.29417	44
TTCGGTC	30	2.671857E-4	119.29417	44
AACGACA	85	0.0	105.30758	43
CAGGACG	70	7.357812E-9	102.252144	44
AATCGCA	35	4.9334083E-4	102.252144	44
TCACTAC	90	3.965397E-10	92.78435	44
CCAGAAT	475	0.0	90.41242	44
CTACAGA	200	0.0	89.47063	44
GTTACCA	40	8.388063E-4	89.47062	44
GTTCGGC	60	2.3646862E-11	85.248985	43
>>END_MODULE