FastQCFastQC Report
Tue 24 May 2016
ERR841008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402253
Sequences flagged as poor quality0
Sequence length24-50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA725993.0221213169470493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA696992.9014013095206876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA649232.7025879455661No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA559192.32777313630163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA533702.221664412532735No Hit
AAAAAAAAAAAAAAAAAAAAAAAA417291.7370776516878115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357751.4892269881648603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203750.8481621211421111No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC179910.7489219495198882No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA157470.6555096403251448No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA153190.6376930323325645No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA138290.5756679250686751No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125440.5221764735021666No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA120700.5024449964262715No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA106200.4420849927130906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104840.43642364064068195No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT94480.39329745867733334No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82390.3429697038571708No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA79420.33060630999316054No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA78220.32561099934103527No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA73120.3043809290695027No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA68720.28606479001170987No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT64700.26933049932709No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG62760.2612547471061541No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58920.24526975301935308No Hit
TAAAAAAAAAAAAAAAAAAAAAAA58190.24223093903931017No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA58170.24214768386177474No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC55440.23078335212818965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG55160.22961777964269373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54140.22537176558838726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG53250.22166691018806095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG51810.2156725374055106No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48580.20222682623353994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG45730.19036296343474232No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA44790.18644997009057748No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC40730.16954916905088682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG39540.1645954859875292No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG38530.1603910995219904No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38100.15860111320497883No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG37800.1573522855419475No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG37490.15606183029014847Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA34210.14240798117433925No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA33490.13941079478306406No Hit
CAAAAAAAAAAAAAAAAAAAAAAA33480.13936916719429635No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA31100.12946180106758112No Hit
AAAAAAAAAAAAAAAAAAAAAAAG29090.12109465572527124No Hit
AGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA28320.11788933139015749No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28250.11759793826878351No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT28010.11659887613835844No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26270.10935567569277672No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC24820.10331967532145865No Hit
AGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAA24660.10265363390117527No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT24540.10215410283596273No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAACT306.91216E-10282.201544
ACCACTC1100.0277.070644
CGGCAAT450.0263.3880644
CTATCAT15650.0240.1868444
CGATCAT150.001884777225.761244
CCAGAAT2300.0213.491644
GGTTATA253.2023345E-5203.1850944
GACACCC850.0199.2010744
AATCGCA700.0193.5096144
CATGACC2000.0177.7869644
GATAGCC600.0171.0422243
CTCGATT209.3565177E-7171.042243
GCGCTAT100.004326206171.042243
TCGACGT100.004326206171.042243
CCGTTTA100.004326206171.042243
AAAGCGG501.4666512E-8169.320944
GCGATTC602.2009772E-10169.3208944
ACTTCTA7950.0166.1261744
ACATCCA950.0160.4092744
GATGTAT755.456968E-12158.0328544
Produced by FastQC (version 0.11.5)