FastQCFastQC Report
Tue 24 May 2016
ERR841007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445338
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC465251.3503754929124514No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA306380.8892596314207779No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT245010.7111348726888335No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT171800.49864483542688703No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC143940.41778194185882483No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG140850.4088133007559781No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA100860.29274341153175687No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA100100.29053753216665534No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA85880.24926436825646717No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA81300.23597104260888194No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG79230.2299629238118292No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA76830.2229969889746666No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC61930.17975014352728239No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC59710.173306653802907No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG59620.1730454312465134No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG50250.1458492606530912No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG48610.14108920518103013No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC46840.13595182823862273No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG44560.1293341901433183No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC43780.12707026132124047No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT41000.11900138680152716No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC39960.11598281503875671No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT38340.11128080902367199No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG36860.10698514920742175No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT36170.10498244294173752No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG35680.10356023124581681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT44300.0145.4171844
AATCGCA4600.0119.0754344
TCACGGT451.0189404E-5105.8448344
ACTTCTA23850.0104.8462944
AACGACA5400.096.4191743
CCTTAGC68200.090.5085843
CTATGCG408.458112E-489.3065844
CTATGGG956.202754E-1087.739844
CGACCAT552.7597176E-586.6003244
CTACCGC552.7597176E-586.6003244
GCGATAC203.790229E-581.24335542
TCGCAAA2500.080.2950943
CTATACG904.280082E-879.3836244
ACATTGG10350.079.3836244
CGTAAGC1101.9808795E-975.77527644
CCTATCA45350.075.1106643
ACGGACA656.318058E-573.2771944
GAACACA28700.071.3622844
TGATATG7950.070.39680544
TCCGGGC3700.069.5122243
Produced by FastQC (version 0.11.5)