##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841007.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3445338
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.12207191282829	39.0	37.0	39.0	34.0	39.0
2	37.757723044879775	39.0	37.0	39.0	35.0	39.0
3	38.052049755350566	39.0	38.0	39.0	35.0	40.0
4	38.473816211936246	39.0	38.0	40.0	35.0	41.0
5	39.09798487115052	40.0	38.0	41.0	35.0	41.0
6	39.12894206606144	40.0	38.0	41.0	35.0	41.0
7	39.1406924371426	40.0	38.0	41.0	35.0	41.0
8	39.08326614108688	40.0	38.0	41.0	35.0	41.0
9	39.12355652769046	40.0	38.0	41.0	35.0	41.0
10	39.087148779016744	40.0	38.0	41.0	35.0	41.0
11	38.96633828088855	40.0	38.0	41.0	35.0	41.0
12	38.92353841625989	40.0	38.0	41.0	35.0	41.0
13	38.87003103904465	40.0	38.0	41.0	35.0	41.0
14	38.80609333539989	40.0	38.0	41.0	35.0	41.0
15	38.79296312872641	40.0	38.0	41.0	35.0	41.0
16	38.74162215724553	40.0	38.0	41.0	35.0	41.0
17	38.732105819516114	40.0	38.0	41.0	35.0	41.0
18	38.70622214714493	40.0	38.0	41.0	35.0	41.0
19	38.692699526142285	40.0	38.0	41.0	35.0	41.0
20	38.58584208574021	40.0	38.0	41.0	35.0	41.0
21	38.50239860356226	40.0	38.0	41.0	35.0	41.0
22	38.44468235046895	40.0	38.0	41.0	34.0	41.0
23	38.32840174171591	40.0	37.0	41.0	34.0	41.0
24	38.144137672414146	40.0	37.0	41.0	34.0	41.0
25	38.072875322875326	40.0	37.0	41.0	34.0	41.0
26	37.956227068327664	39.0	36.0	41.0	33.0	41.0
27	37.85736663870379	39.0	36.0	41.0	33.0	41.0
28	37.70491985475341	39.0	36.0	41.0	33.0	41.0
29	37.67641327743408	39.0	36.0	41.0	33.0	41.0
30	37.626770029773176	39.0	36.0	41.0	33.0	41.0
31	37.53305969464442	39.0	36.0	41.0	33.0	41.0
32	37.520007261301465	39.0	35.0	41.0	33.0	41.0
33	37.363328457266114	39.0	35.0	40.0	33.0	41.0
34	37.28020561191599	38.0	35.0	40.0	32.0	41.0
35	37.194949723650666	38.0	35.0	40.0	32.0	41.0
36	37.15347424427204	38.0	35.0	40.0	32.0	41.0
37	37.05989638580533	38.0	35.0	40.0	32.0	41.0
38	36.87635203530362	38.0	35.0	40.0	31.0	41.0
39	36.84815927771125	38.0	35.0	40.0	31.0	41.0
40	36.64301862772941	38.0	34.0	40.0	31.0	41.0
41	36.554588290793795	38.0	34.0	40.0	31.0	41.0
42	36.3842913107742	37.0	34.0	40.0	31.0	41.0
43	36.2294058155567	37.0	34.0	40.0	30.0	41.0
44	35.99892048286919	37.0	34.0	40.0	30.0	41.0
45	35.819068889725365	37.0	34.0	40.0	29.0	41.0
46	35.527524830113975	36.0	33.0	40.0	29.0	41.0
47	35.73053010863283	37.0	34.0	40.0	29.0	41.0
48	36.58569217271306	38.0	35.0	40.0	31.0	41.0
49	37.33737411685749	39.0	35.0	40.0	33.0	41.0
50	37.23597563632244	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	11.0
22	47.0
23	119.0
24	275.0
25	551.0
26	1038.0
27	2149.0
28	4717.0
29	10167.0
30	20973.0
31	41458.0
32	75828.0
33	129277.0
34	202294.0
35	214273.0
36	345949.0
37	561204.0
38	555156.0
39	547504.0
40	732346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.53223747568453	17.079108058483666	33.3886544658318
2	25.585356211785314	22.32001620740839	31.55733922187025	20.537288358936046
3	26.230837148633896	21.95990640105557	24.630703867080676	27.17855258322986
4	20.651471640808534	25.66456469582955	28.91100960196068	24.772954061401233
5	17.052985802844304	28.100377960014374	29.30432369770397	25.54231253943735
6	21.88728653037815	28.023607553163142	26.395204186062443	23.693901730396263
7	24.898515036841086	23.777347824799772	24.729242820298037	26.5948943180611
8	21.421294514500463	23.788261122711326	29.534228572058822	25.256215790729385
9	22.342597446172192	27.024373225500664	27.825310608131915	22.807718720195233
10	28.025784407799758	20.889213191855198	28.20344477087589	22.881557629469153
11	23.55574402279254	22.66633926772932	26.69891894496273	27.07899776451541
12	21.06736697531563	25.010231216792082	27.654935451906315	26.267466355985974
13	23.166232166481198	23.78280447375555	26.567378875454313	26.483584484308942
14	18.654918617563794	25.68975816015729	26.523464461251695	29.131858761027217
15	21.739260415088445	29.7292166980424	26.203263656570126	22.32825923029903
16	20.91783157414454	32.27979373866947	23.46530296882338	23.337071718362612
17	22.74978536213283	32.79327021035382	20.82480731933993	23.63213710817342
18	21.642753192865257	28.095356681985916	23.791947263229325	26.4699428619195
19	24.635957342937033	22.616474784186632	25.66177832189469	27.085789550981644
20	23.411549171663275	23.653296135241302	27.12964591572728	25.805508777368143
21	22.48206126655788	29.774727472311863	24.310474037670616	23.432737223459643
22	24.609051419628493	26.899973239200335	23.19435712838624	25.296618212784928
23	26.868452384062174	24.142943304836855	26.98588643552534	22.002717875575634
24	22.504584455864705	27.727729470954664	24.7607636754362	25.00692239774443
25	23.02500635833969	24.401732735066066	25.98921765588432	26.584043250709914
26	23.946601261634004	30.674290089062378	26.756817437069248	18.62229121223437
27	24.789402411953	27.404113281759535	22.45109121483648	25.355393091450985
28	24.568450952010064	25.338072933893358	23.609624305449355	26.483851808647223
29	24.950391844088397	27.337304767061436	26.334890736900512	21.377412651949655
30	26.575587193218812	27.542016857083958	26.43391289547745	19.448483054219786
31	23.46376548681813	29.007326341241964	24.529448160631343	22.999460011308567
32	23.03053949886055	27.502605722904594	26.153751966856305	23.31310281137856
33	22.983208394974326	24.57111517569332	29.77622746991618	22.669448959416172
34	22.801763092876996	28.747605666819865	26.487200920410974	21.963430319892165
35	21.78845473887934	24.88034166826914	25.183798671290063	28.147404921561453
36	21.918723658755052	25.582950918279707	26.46639754879984	26.0319278741654
37	20.081558204840768	31.022088628172668	24.7864385162608	24.109914650725763
38	24.0896390402208	25.676507347969878	24.14549599586507	26.08835761594425
39	21.081043637855338	29.475896434407854	26.016624156725527	23.42643577101128
40	19.741282069589758	25.832571039050066	27.939769092870335	26.48637779848984
41	20.96239025503071	30.05078543339926	25.067527298904817	23.91929701266522
42	21.55181641398342	30.866430397340455	24.069669193661788	23.512083995014333
43	19.578022510557997	25.474764181691047	25.57389149263146	29.373321815119503
44	19.122947064925967	26.491754577422093	25.033817010899583	29.351481346752355
45	20.039224548722498	26.21669700791045	29.20373664683831	24.540341796528743
46	18.87305589165859	28.999411886638736	27.9595278381692	24.168004383533468
47	21.144723308848693	29.18981173058139	26.871311367769035	22.794153592800885
48	23.0544325792943	27.63181541511036	23.820668694800094	25.493083310795246
49	18.57973176540963	29.036011423860984	24.027312219373144	28.356944591356235
50	19.982746751945346	30.450937165064783	27.123151593311317	22.443164489678548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25914.0
1	23751.5
2	28788.0
3	34478.5
4	30064.0
5	19262.5
6	8393.0
7	5519.0
8	5691.5
9	5697.5
10	5640.5
11	5533.5
12	5044.0
13	4472.5
14	4744.0
15	5199.0
16	4951.0
17	4612.5
18	4522.5
19	5244.5
20	6530.0
21	7761.5
22	9228.5
23	12001.0
24	15268.5
25	18775.5
26	23335.0
27	28852.5
28	34401.5
29	39304.0
30	45790.0
31	61117.5
32	85466.5
33	97682.0
34	111007.0
35	136405.5
36	145908.5
37	144553.0
38	146731.5
39	152444.5
40	164655.5
41	185081.0
42	209830.5
43	233357.0
44	247851.0
45	259034.0
46	275675.0
47	282493.0
48	303681.5
49	342003.5
50	381821.0
51	394135.5
52	351170.0
53	303424.5
54	268970.5
55	245186.0
56	229954.0
57	212225.5
58	187821.5
59	164339.0
60	148614.5
61	130215.0
62	111213.0
63	90617.0
64	70870.0
65	58509.5
66	46180.5
67	32969.5
68	22219.5
69	15370.5
70	11603.0
71	8725.0
72	6504.0
73	4955.0
74	3660.5
75	2618.5
76	2081.0
77	1541.5
78	1067.5
79	740.5
80	618.0
81	549.5
82	412.0
83	239.5
84	82.0
85	27.5
86	13.5
87	17.5
88	20.5
89	19.0
90	17.5
91	8.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	16770.0
25	16802.0
26	15597.0
27	22045.0
28	18325.0
29	18567.0
30	35311.0
31	18763.0
32	23017.0
33	18111.0
34	17850.0
35	18893.0
36	18851.0
37	21208.0
38	18121.0
39	23860.0
40	24130.0
41	25506.0
42	36103.0
43	68588.0
44	176791.0
45	302813.0
46	402838.0
47	389351.0
48	600475.0
49	522847.0
50	573805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.901245593502356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68634329746658	9.635468573612451
2	11.041888722730153	2.849082364561315
3	3.8463779853667406	1.488692011039714
4	1.7064982581449977	0.880638125328504
5	0.9651229593273578	0.6225644163105015
6	0.6377308636631581	0.4936513496804862
7	0.4606521911297856	0.41600909356652466
8	0.34352804467578546	0.35455517380943663
9	0.2625624777804429	0.30486447085355994
>10	2.852063825215055	8.693834914313063
>50	1.4433217568177008	13.746869746677342
>100	1.6449427627145434	35.470812676921284
>500	0.06296587727167827	5.59301196357303
>1k	0.040781008595646547	10.068322261813798
>5k	0.002609984550121379	2.334946333432774
>10k+	0.002609984550121379	7.046676524506203
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	46525	1.3503754929124514	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	30638	0.8892596314207779	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	24501	0.7111348726888335	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	17180	0.49864483542688703	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	14394	0.41778194185882483	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	14085	0.4088133007559781	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10086	0.29274341153175687	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	10010	0.29053753216665534	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	8588	0.24926436825646717	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	8130	0.23597104260888194	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	7923	0.2299629238118292	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	7683	0.2229969889746666	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	6193	0.17975014352728239	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	5971	0.173306653802907	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	5962	0.1730454312465134	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	5025	0.1458492606530912	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4861	0.14108920518103013	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	4684	0.13595182823862273	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	4456	0.1293341901433183	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	4378	0.12707026132124047	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	4100	0.11900138680152716	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	3996	0.11598281503875671	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	3834	0.11128080902367199	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	3686	0.10698514920742175	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	3617	0.10498244294173752	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	3568	0.10356023124581681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.035294069841623664	0.0	0.0	0.0	0.0
2	0.06568296056874535	0.0	0.0	0.0	0.0
3	0.08245925363491187	0.0	0.0	0.0	0.0
4	0.10115117878129809	0.0	0.0	0.0	0.0
5	0.11778234820502372	0.0	0.0	0.0	0.0
6	0.14808416474668087	0.0	0.0	0.0	0.0
7	0.1672695102773661	0.0	0.0	0.0	0.0
8	0.19092466399523067	0.0	0.0	0.0	0.0
9	0.21005196006893953	0.0	0.0	0.0	0.0
10	0.22772801971823955	0.0	0.0	0.0	0.0
11	0.24398186767161886	0.0	0.0	0.0	0.0
12	0.2671145762766962	0.0	0.0	0.0	0.0
13	0.2871126142050504	0.0	0.0	0.0	0.0
14	0.30754602306072726	0.0	0.0	0.0	0.0
15	0.3289952974134904	0.0	0.0	0.0	0.0
16	0.34818064294417556	0.0	0.0	0.0	0.0
17	0.3734031320004017	0.0	0.0	0.0	0.0
18	0.4044305667542633	0.0	0.0	0.0	0.0
19	0.4260539894779554	0.0	0.0	0.0	0.0
20	0.4469227692609549	0.0	0.0	0.0	0.0
21	0.47037474987940225	0.0	0.0	0.0	0.0
22	0.49014058997985105	0.0	0.0	0.0	0.0
23	0.515014782294219	0.0	0.0	0.0	0.0
24	0.536435031918494	0.0	0.0	0.0	0.0
25	0.556897465502659	0.0	0.0	0.0	0.0
26	0.5849643779507264	0.0	0.0	0.0	0.0
27	0.6071102457872057	0.0	0.0	0.0	0.0
28	0.6338420207248171	0.0	0.0	0.0	0.0
29	0.6602835483775467	0.0	0.0	0.0	0.0
30	0.6852738396058674	0.0	0.0	0.0	0.0
31	0.7098577846353537	0.0	0.0	0.0	0.0
32	0.7366185843014531	0.0	0.0	0.0	0.0
33	0.7628859635832537	0.0	0.0	0.0	0.0
34	0.7863669689301891	0.0	0.0	0.0	0.0
35	0.8082516142102749	0.0	0.0	0.0	0.0
36	0.8276691575688655	0.0	0.0	0.0	0.0
37	0.8450549699332838	0.0	0.0	0.0	0.0
38	0.8559682678448385	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	4430	0.0	145.41718	44
AATCGCA	460	0.0	119.07543	44
TCACGGT	45	1.0189404E-5	105.84483	44
ACTTCTA	2385	0.0	104.84629	44
AACGACA	540	0.0	96.41917	43
CCTTAGC	6820	0.0	90.50858	43
CTATGCG	40	8.458112E-4	89.30658	44
CTATGGG	95	6.202754E-10	87.7398	44
CGACCAT	55	2.7597176E-5	86.60032	44
CTACCGC	55	2.7597176E-5	86.60032	44
GCGATAC	20	3.790229E-5	81.243355	42
TCGCAAA	250	0.0	80.29509	43
CTATACG	90	4.280082E-8	79.38362	44
ACATTGG	1035	0.0	79.38362	44
CGTAAGC	110	1.9808795E-9	75.775276	44
CCTATCA	4535	0.0	75.11066	43
ACGGACA	65	6.318058E-5	73.27719	44
GAACACA	2870	0.0	71.36228	44
TGATATG	795	0.0	70.396805	44
TCCGGGC	370	0.0	69.51222	43
>>END_MODULE