FastQCFastQC Report
Tue 24 May 2016
ERR841006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000587
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG241490.6036364163558998No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC226200.5654170250515737No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA152890.3821689167114726No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA143260.35809744919933No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA121030.3025306036339167No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA103680.2591619679811987No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT94860.23711520334390926No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA92330.23079113140146681No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG86720.2167681892682249No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT86650.2165932149457067No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78830.19704608348724825No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG74210.185497778201049No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT73720.1842729579434218No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG65660.1641259145220439No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC64960.1623761712968622No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG64060.16012650143591428No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA63940.15982654545445457No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC56300.14072934796818568No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC55150.13785476981253No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT49880.12468170296009061No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA49720.12428176165147765No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG47350.11835763101764817No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA47270.11815766036334169No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46850.11710781442823266No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45780.11443320692688348No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAA45690.1142082399407887No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC44830.11205855540699403No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44500.1112336764579798No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA44100.11023382318644738No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG43490.10870904694736047No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA43150.10785917166655794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT152.2177817E-5222.832844
CTATCAT20350.0120.4501644
ACATCGC1300.094.2754144
CCTTAGC38450.088.015743
GTATCGT1052.3646862E-1184.8886944
GAGCGTA203.5896715E-582.1394742
CGTGCGC5350.081.2194344
CTTCGCG505.996844E-875.62466443
TTTAACG1455.456968E-1269.1550144
CGGCTAC551.1605334E-768.74969543
CAATGAA33800.067.49538443
ACACGCG150.002620174567.1739941
ATACTCG1003.417881E-966.8498444
CTTTCGT1001.4080979E-766.8498444
TCGGATC1206.673872E-964.992944
CCTATCA21700.063.89180843
ACCTTAG37850.063.8018742
ACGGCGA300.00337394463.02055443
GGCCACG1300.063.0205543
TCGTGCG12150.059.64908243
Produced by FastQC (version 0.11.5)