##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841006.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000587
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.73895605819846	39.0	37.0	39.0	32.0	39.0
2	37.500441310237726	39.0	37.0	39.0	35.0	39.0
3	37.876943308569466	39.0	38.0	39.0	35.0	39.0
4	38.16766139569018	39.0	37.0	40.0	35.0	41.0
5	38.92681224030373	40.0	38.0	41.0	35.0	41.0
6	38.87290115175598	40.0	38.0	41.0	35.0	41.0
7	38.941857032480485	40.0	38.0	41.0	35.0	41.0
8	38.854500851999966	40.0	38.0	41.0	35.0	41.0
9	38.897171590069156	40.0	38.0	41.0	35.0	41.0
10	38.87211576701119	40.0	38.0	41.0	35.0	41.0
11	38.7550644442928	40.0	38.0	41.0	35.0	41.0
12	38.687494610165956	40.0	38.0	41.0	35.0	41.0
13	38.629337144774006	40.0	38.0	41.0	35.0	41.0
14	38.620859638847996	40.0	38.0	41.0	35.0	41.0
15	38.63913370712848	40.0	38.0	41.0	35.0	41.0
16	38.58162889595952	40.0	38.0	41.0	35.0	41.0
17	38.55922893315406	40.0	38.0	41.0	35.0	41.0
18	38.46045492823928	40.0	37.0	41.0	35.0	41.0
19	38.34413574807897	40.0	37.0	41.0	34.0	41.0
20	38.23079263118137	40.0	36.0	41.0	34.0	41.0
21	38.09418467839844	40.0	36.0	41.0	34.0	41.0
22	37.99244985798334	40.0	36.0	41.0	34.0	41.0
23	37.89877160526693	39.0	35.0	41.0	34.0	41.0
24	37.76605483145348	39.0	35.0	41.0	33.0	41.0
25	37.638618513997145	39.0	35.0	41.0	33.0	41.0
26	37.50836677957404	39.0	35.0	41.0	33.0	41.0
27	37.324287022680636	38.0	35.0	40.0	33.0	41.0
28	37.14972235746585	38.0	35.0	40.0	33.0	41.0
29	37.093219275904296	38.0	35.0	40.0	33.0	41.0
30	37.072083659473606	38.0	35.0	40.0	33.0	41.0
31	36.95100786023821	38.0	35.0	40.0	33.0	41.0
32	36.89950276027194	38.0	35.0	40.0	32.0	41.0
33	36.75869803464113	38.0	35.0	40.0	32.0	41.0
34	36.64975465595176	38.0	35.0	40.0	32.0	41.0
35	36.59202914100759	37.0	35.0	40.0	32.0	41.0
36	36.54087621149662	37.0	35.0	40.0	32.0	41.0
37	36.43716828974108	37.0	34.0	40.0	31.0	41.0
38	36.30439101191874	37.0	34.0	40.0	31.0	41.0
39	36.21092448234471	36.0	34.0	40.0	31.0	41.0
40	35.93340717219616	36.0	34.0	40.0	31.0	41.0
41	35.80887206888592	36.0	34.0	39.0	31.0	41.0
42	35.670594962851766	36.0	34.0	39.0	30.0	41.0
43	35.57401065698263	36.0	34.0	39.0	30.0	41.0
44	35.31232812762575	35.0	34.0	39.0	29.0	41.0
45	35.09321244043077	35.0	33.0	39.0	29.0	41.0
46	34.771882360316944	35.0	33.0	39.0	28.0	41.0
47	34.81428685843502	35.0	33.0	38.0	29.0	40.0
48	35.57675259950603	36.0	34.0	39.0	31.0	40.0
49	36.3451782617641	37.0	35.0	39.0	32.0	41.0
50	36.23364933771159	37.0	35.0	39.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	14.0
21	50.0
22	151.0
23	296.0
24	604.0
25	1285.0
26	2187.0
27	4307.0
28	8738.0
29	17203.0
30	32543.0
31	61576.0
32	112987.0
33	200615.0
34	319609.0
35	290067.0
36	451590.0
37	687826.0
38	614529.0
39	574015.0
40	620395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.287855007277685	19.29096905029187	27.421175942430448
2	29.251632322956606	23.120707036242433	30.209816709397895	17.417843931403066
3	31.62083464251621	19.643992244138172	22.296627969845424	26.438545143500193
4	20.77412639695125	27.136767679343055	27.19735878759792	24.891747136107774
5	20.324692351397434	30.289155066493993	25.137086132610037	24.249066449498535
6	22.87254345424809	37.13635024060219	22.43233305512416	17.55877325002556
7	28.857590148645684	33.383775930882145	19.620420703261797	18.138213217210374
8	23.73549181657592	30.309802036551137	25.202126587923225	20.752579558949723
9	23.29878090390235	31.437661523171474	26.57762473357035	18.68593283935582
10	27.377957284768463	27.550157014458126	26.160760908336698	18.91112479243671
11	24.073817167330695	29.179942843387735	25.506356942118746	21.239883047162827
12	22.21376512996718	29.463651209185056	27.870285035671017	20.452298625176756
13	20.997968548115566	30.272432520527616	27.25330057814016	21.476298353216666
14	16.741768145524645	34.933198553112334	25.92517048123188	22.399862820131144
15	17.87142736803374	37.634252173493536	25.030676748187204	19.463643710285517
16	20.118372628816722	39.61013721236409	21.383836921931707	18.887653236887488
17	20.03748449914975	40.711225627639145	18.707354695698406	20.5439351775127
18	21.45402662159328	32.09653983277954	22.677872022280727	23.77156152334645
19	22.21416507127579	29.364865705957648	25.785141030553767	22.63582819221279
20	22.744212286846903	29.481523586413694	25.27291619954772	22.501347927191684
21	22.484300428912057	31.73429299250335	23.84595060674846	21.93545597183613
22	22.590884787657412	33.59784451631723	22.58853513246931	21.222735563556046
23	23.898117951190663	30.646602611066825	24.257365231652255	21.197914206090257
24	22.809227745828302	30.807179046474932	24.38554642106271	21.998046786634063
25	21.127069190899054	31.263711051817662	26.05890154780028	21.55031820948301
26	22.60848855992088	33.98823311920331	24.600741289245246	18.802537031630564
27	23.53573999668985	31.53531658152671	22.54320205432508	22.385741367458362
28	23.172205808499452	31.49512126712691	23.20459616026174	22.1280767641119
29	23.37275885789023	33.581632222886	22.76605545621772	20.279553463006053
30	23.810192436338323	32.06918905947738	24.26181106179896	19.85880744238533
31	21.94303181065837	32.615023354856255	24.42912462063261	21.01282021385277
32	21.999900721039854	32.527457497515385	25.37121353742553	20.101428244019235
33	22.394810887973613	30.572616008639642	25.22357471865899	21.808998384727754
34	20.00922087623565	33.406289421662564	25.011908732121462	21.57258096998033
35	21.900973937675676	31.321171041597268	24.713516304411066	22.064338716315994
36	21.725111754437666	32.009082663524616	24.849574178584096	21.416231403453626
37	20.5550242123275	32.89520158186291	23.358028215011505	23.19174599079808
38	22.303838026710125	31.90172220917867	24.21561451240902	21.578825251702185
39	23.002169974074842	33.7832939239058	23.758881020593904	19.455655081425455
40	20.295018543942547	32.22176365387261	25.486367885990553	21.996849916194293
41	20.94215298877313	34.90372081520796	23.03434670727999	21.119779488738917
42	21.968015847465846	35.37030310229523	21.970053018082613	20.69162803215631
43	20.421982275626497	30.839658787905783	25.070343479970923	23.668015456496796
44	20.95475150534306	32.21584886782502	23.686979383224987	23.14242024360694
45	21.477748755918828	30.928829287556397	26.094181928538507	21.49924002798627
46	20.710159713089237	33.345468431409145	26.156420226809967	19.787951628691648
47	23.04193757545756	31.869311128397776	26.002387377063286	19.08636391908138
48	22.813400974746617	32.70053202453325	22.32104334335909	22.16502365736104
49	19.056822451584818	34.92708827335404	22.45746319461159	23.558626080449553
50	19.39850976325666	34.201256430035734	26.294543058231078	20.105690748476533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67348.0
1	56324.5
2	43751.5
3	39106.5
4	33844.0
5	36051.0
6	38384.5
7	40413.0
8	53445.5
9	64578.5
10	62983.5
11	48832.5
12	34353.0
13	26676.0
14	21869.5
15	19389.5
16	17304.5
17	16530.0
18	17551.5
19	19472.5
20	21601.5
21	25300.5
22	28674.5
23	32438.0
24	37743.0
25	42174.5
26	46357.0
27	52365.0
28	60546.5
29	70015.5
30	80206.0
31	94332.0
32	117245.0
33	142392.5
34	167768.0
35	185072.0
36	197538.0
37	211118.5
38	217774.5
39	230275.0
40	246648.5
41	262616.0
42	284610.0
43	301012.0
44	306751.0
45	306557.0
46	296795.0
47	280080.0
48	279881.0
49	305036.5
50	342800.5
51	360763.0
52	336635.0
53	295872.0
54	258484.5
55	237504.0
56	223165.0
57	202933.0
58	172950.0
59	144355.0
60	129617.0
61	111750.0
62	91885.0
63	72748.5
64	56367.0
65	44146.0
66	30567.0
67	19895.0
68	14247.5
69	10392.0
70	7787.5
71	5947.5
72	4650.5
73	3503.5
74	2355.0
75	1436.5
76	1031.0
77	612.5
78	338.5
79	288.0
80	175.0
81	83.0
82	45.5
83	22.5
84	12.5
85	9.0
86	11.0
87	10.0
88	8.0
89	7.5
90	6.5
91	5.5
92	4.5
93	4.0
94	6.0
95	8.5
96	9.0
97	9.0
98	4.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	21133.0
25	19866.0
26	20192.0
27	24650.0
28	24542.0
29	23003.0
30	50650.0
31	29243.0
32	32523.0
33	30627.0
34	28747.0
35	27324.0
36	24282.0
37	23455.0
38	23072.0
39	35468.0
40	39813.0
41	36771.0
42	50295.0
43	84698.0
44	200117.0
45	342832.0
46	459938.0
47	421307.0
48	667188.0
49	547152.0
50	711699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.389488263477192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58168246995321	8.992499031049135
2	11.985670305242076	2.969926431534076
3	4.032413553723129	1.4987862119201716
4	1.834485306948173	0.9091333671982298
5	1.0524652835387072	0.6519753139060005
6	0.7083867236751306	0.5265929399385655
7	0.5183058114027062	0.4495080637086095
8	0.37237014006498914	0.3690780384003633
9	0.29751173141142107	0.3317416294111722
>10	3.0995349066604563	9.08395276520109
>50	1.6194217727079412	14.813852479069936
>100	1.790537790059342	36.94646161137741
>500	0.06360164680419574	5.359995655561876
>1k	0.037858123097735556	9.794663730923284
>5k	0.0039372448021644975	3.592454874323307
>10k+	0.0018171899086913067	3.709377856476781
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	24149	0.6036364163558998	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	22620	0.5654170250515737	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	15289	0.3821689167114726	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	14326	0.35809744919933	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	12103	0.3025306036339167	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	10368	0.2591619679811987	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	9486	0.23711520334390926	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	9233	0.23079113140146681	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	8672	0.2167681892682249	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8665	0.2165932149457067	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7883	0.19704608348724825	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	7421	0.185497778201049	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	7372	0.1842729579434218	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	6566	0.1641259145220439	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	6496	0.1623761712968622	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	6406	0.16012650143591428	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	6394	0.15982654545445457	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	5630	0.14072934796818568	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5515	0.13785476981253	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4988	0.12468170296009061	No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	4972	0.12428176165147765	No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG	4735	0.11835763101764817	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	4727	0.11815766036334169	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4685	0.11710781442823266	No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4578	0.11443320692688348	No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAA	4569	0.1142082399407887	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	4483	0.11205855540699403	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4450	0.1112336764579798	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	4410	0.11023382318644738	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	4349	0.10870904694736047	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA	4315	0.10785917166655794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.030320550459220108	0.0	0.0	0.0	0.0
2	0.04814293502428518	0.0	0.0	0.0	0.0
3	0.06674020587478788	0.0	0.0	0.0	0.0
4	0.0961108957260522	0.0	0.0	0.0	0.0
5	0.13200562817406544	0.0	0.0	0.0	0.0
6	0.30218065498888036	0.0	0.0	0.0	0.0
7	0.5056008030821477	0.0	0.0	0.0	0.0
8	0.570366298745659	0.0	0.0	0.0	0.0
9	0.6358066953674548	0.0	0.0	0.0	0.0
10	0.6769756538228014	0.0	0.0	0.0	0.0
11	0.7113206136999395	0.0	0.0	0.0	0.0
12	0.7479902324333904	0.0	0.0	0.0	0.0
13	0.7816852876840323	0.0	0.0	0.0	0.0
14	0.8212044882413506	0.0	0.0	0.0	0.0
15	0.8627733880053102	0.0	0.0	0.0	0.0
16	0.9042423024421167	0.0	0.0	0.0	0.0
17	0.9482858390531189	0.0	0.0	0.0	0.0
18	0.9930792656177706	0.0	0.0	0.0	0.0
19	1.0378726921824222	0.0	0.0	0.0	0.0
20	1.0736674393032823	0.0	0.0	0.0	0.0
21	1.10476287604794	0.0	0.0	0.0	0.0
22	1.1331837052912486	0.0	0.0	0.0	0.0
23	1.1617795088570753	0.0	0.0	0.0	0.0
24	1.189100499501698	0.0	0.0	0.0	0.0
25	1.2170713947728171	0.0	0.0	0.0	0.0
26	1.2483168095082047	0.0	0.0	0.0	0.0
27	1.273813067932281	0.0	0.0	0.0	0.0
28	1.3005591429457728	0.0	0.0	0.0	0.0
29	1.3326794292937512	0.0	0.0	0.0	0.0
30	1.3647247266463647	0.0	0.0	0.0	0.0
31	1.3937954605161693	0.0	0.0	0.0	0.0
32	1.4228162017223973	0.0	0.0	0.0	0.0
33	1.4526118292140628	0.0	0.0	0.0	0.0
34	1.4844071632487932	0.0	0.0	2.4996331788310065E-5	0.0
35	1.5110782492669201	0.0	0.0	2.4996331788310065E-5	0.0
36	1.5334499662174577	0.0	0.0	2.4996331788310065E-5	0.0
37	1.553322049989164	0.0	0.0	2.4996331788310065E-5	0.0
38	1.5686697977071864	0.0	0.0	2.4996331788310065E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGT	15	2.2177817E-5	222.8328	44
CTATCAT	2035	0.0	120.45016	44
ACATCGC	130	0.0	94.27541	44
CCTTAGC	3845	0.0	88.0157	43
GTATCGT	105	2.3646862E-11	84.88869	44
GAGCGTA	20	3.5896715E-5	82.13947	42
CGTGCGC	535	0.0	81.21943	44
CTTCGCG	50	5.996844E-8	75.624664	43
TTTAACG	145	5.456968E-12	69.15501	44
CGGCTAC	55	1.1605334E-7	68.749695	43
CAATGAA	3380	0.0	67.495384	43
ACACGCG	15	0.0026201745	67.17399	41
ATACTCG	100	3.417881E-9	66.84984	44
CTTTCGT	100	1.4080979E-7	66.84984	44
TCGGATC	120	6.673872E-9	64.9929	44
CCTATCA	2170	0.0	63.891808	43
ACCTTAG	3785	0.0	63.80187	42
ACGGCGA	30	0.003373944	63.020554	43
GGCCACG	130	0.0	63.02055	43
TCGTGCG	1215	0.0	59.649082	43
>>END_MODULE