FastQCFastQC Report
Tue 24 May 2016
ERR841005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999774
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC187471.8751237779738221No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA124481.245081388393777No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT96520.9654181845096992No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT70480.7049593208065023No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC54430.5444230396069512No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA34530.3453780554405296No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA29990.299967792721155No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA24970.24975644495656016No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA24620.24625565377775377No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA22950.22955187872459176No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTTCA19640.196444396433594No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC19540.19544417038250644No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC18570.18574197768695724No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17540.17543964936075554No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT17220.1722389259972754No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG16490.1649372758243363No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC16270.16273677851194368No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC15790.15793569346672348No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA15500.1550350379185696No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA15300.1530345858163945No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC14700.14703322950986922No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA14290.1429323027004103No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC14070.1407318053880177No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG14040.14043173757269142No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG13420.13423033605594867No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG13410.1341303134508399No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC13350.13353017782018736No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG13170.1317297709282298No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA12430.12432809815018193No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG12060.12062726176115801No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT11360.11362567940354519No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT11170.11172524990647886No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTT11060.11062500125028256No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT10850.10852452654299871No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG10800.10802441351745494No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC10790.1079243909123462No Hit
AGTTGGCCCTGTGGTGTTCCGAAGCCGGTTACGTACGGCCTGAGGGCCA10680.10682414225614989No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGC10360.10362341889266974No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT10250.10252317023647345No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10250.10252317023647345No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG10040.10042269552918959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGT109.097896E-4287.8910244
CGTGCGC109.097896E-4287.8910244
ATGTACA157.965622E-6287.8910244
TGCGATT206.9630914E-8287.8910244
GTCGTCA157.965622E-6287.8910244
ATATGTA202.5105568E-5215.9182744
CGGTCTG202.5105568E-5215.9182744
GTCCCCG354.5802153E-9205.6364444
AATCGCA1200.0203.9228244
GGCCGAA150.0030625628191.9273544
GCCCGTC150.0030625628191.9273544
TAATAAC256.112312E-5172.7346344
CTATCAT18400.0171.3264644
CCAACAA603.6379788E-12167.9364344
AATTCCC351.1329957E-6164.5091644
CCTATTA800.0161.938744
ACTTCTA4750.0151.5215944
ACATTGG2700.0143.9455344
GTTAACA651.1878001E-9132.8727944
GGAATTC352.33512E-4123.3818744
Produced by FastQC (version 0.11.5)