##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841005.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	999774
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.92268052579883	39.0	37.0	39.0	33.0	39.0
2	37.61222936383623	39.0	37.0	39.0	35.0	39.0
3	37.90445240624381	39.0	38.0	39.0	35.0	39.0
4	38.593077035410005	39.0	38.0	41.0	35.0	41.0
5	39.109502747620965	40.0	38.0	41.0	35.0	41.0
6	39.082493643563446	40.0	38.0	41.0	35.0	41.0
7	39.06629298221398	40.0	38.0	41.0	35.0	41.0
8	39.025396739663165	40.0	38.0	41.0	35.0	41.0
9	39.021244801325096	40.0	38.0	41.0	35.0	41.0
10	38.927602638196234	40.0	38.0	41.0	35.0	41.0
11	38.88011090506455	40.0	38.0	41.0	35.0	41.0
12	38.81428302796432	40.0	38.0	41.0	35.0	41.0
13	38.678002228503644	40.0	38.0	41.0	35.0	41.0
14	38.61049197118549	40.0	38.0	41.0	35.0	41.0
15	38.56079674006325	40.0	38.0	41.0	35.0	41.0
16	38.53108802589385	40.0	38.0	41.0	35.0	41.0
17	38.52929662103635	40.0	38.0	41.0	35.0	41.0
18	38.55008932018636	40.0	38.0	41.0	35.0	41.0
19	38.54597038930798	40.0	38.0	41.0	35.0	41.0
20	38.46139727578433	40.0	37.0	41.0	35.0	41.0
21	38.33030564907669	40.0	37.0	41.0	34.0	41.0
22	38.27565529809737	40.0	37.0	41.0	34.0	41.0
23	38.12757283145991	40.0	37.0	41.0	34.0	41.0
24	37.92678845419065	39.0	36.0	41.0	33.0	41.0
25	37.831272548739484	39.0	36.0	41.0	33.0	41.0
26	37.71036276245889	39.0	36.0	41.0	33.0	41.0
27	37.61079598109084	39.0	36.0	41.0	33.0	41.0
28	37.45171304431627	39.0	35.0	40.0	33.0	41.0
29	37.363987794995616	39.0	35.0	40.0	33.0	41.0
30	37.32568505761953	38.0	35.0	40.0	33.0	41.0
31	37.147594385239934	38.0	35.0	40.0	32.0	41.0
32	37.10832322379872	38.0	35.0	40.0	32.0	41.0
33	36.970940984397124	38.0	35.0	40.0	32.0	41.0
34	36.89625322969805	38.0	35.0	40.0	32.0	41.0
35	36.66663365654548	38.0	35.0	40.0	31.0	41.0
36	36.66475582598707	38.0	35.0	40.0	31.0	41.0
37	36.60683839871294	38.0	35.0	40.0	31.0	41.0
38	36.36136941837003	37.0	34.0	40.0	31.0	41.0
39	36.318736713370846	37.0	34.0	40.0	31.0	41.0
40	36.0221889489416	36.0	34.0	40.0	31.0	41.0
41	35.93048353220025	36.0	34.0	40.0	30.0	41.0
42	35.81487660521044	36.0	34.0	40.0	30.0	41.0
43	35.64452172872298	36.0	34.0	39.0	30.0	41.0
44	35.35743086878856	36.0	33.0	39.0	29.0	41.0
45	35.08312656021618	35.0	33.0	39.0	29.0	41.0
46	34.78226746980002	35.0	33.0	39.0	28.0	41.0
47	34.87196026521255	35.0	33.0	39.0	29.0	40.0
48	35.79944489423276	36.0	34.0	39.0	31.0	41.0
49	36.27536137193775	37.0	35.0	39.0	31.0	41.0
50	36.608011707948464	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	17.0
22	36.0
23	66.0
24	138.0
25	238.0
26	462.0
27	938.0
28	1811.0
29	3833.0
30	7595.0
31	14312.0
32	25804.0
33	43823.0
34	68497.0
35	68641.0
36	113765.0
37	171541.0
38	165175.0
39	150706.0
40	162372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.15234843074535	20.15825576580307	40.689395803451575
2	22.4584756154891	20.787698019752465	34.48739415107814	22.266432213680293
3	21.48105471836635	23.48730813163775	27.967520659669088	27.06411649032681
4	19.597328996353173	25.362331887006462	33.05977150836089	21.980567608279472
5	17.41333541380352	26.71823832186074	29.681508020812704	26.18691824352304
6	21.0988683442458	23.606735122137604	26.90318011870683	28.39121641490977
7	24.704583235811292	22.346450297767294	21.788424183865555	31.16054228255586
8	24.168862162848804	23.25855643375403	27.812085531330084	24.760495872067086
9	23.930008181849097	27.06421651293192	26.341953281441604	22.663822023777374
10	28.352607689337788	19.481602842242346	25.834938696145326	26.330850772274534
11	26.343453620518236	23.0446080814264	23.76026982097954	26.85166847707582
12	24.990847931632548	25.50136330810763	23.025003650825088	26.48278510943473
13	26.67422837561289	21.808128637071977	25.03315749359355	26.484485493721582
14	23.82778507942795	23.68165205336406	22.72683626499589	29.7637266022121
15	22.78785005411223	32.448833436356615	23.18684022589105	21.576476283640105
16	21.9854687159298	31.26676628918135	20.692076409268495	26.055688585620352
17	21.863041047276685	34.67983764330739	20.088039897016728	23.369081412399204
18	21.997871518963287	27.141834054496318	22.104295570799	28.7559988557414
19	22.85356490566868	24.24487934273146	21.98136778912034	30.920187962479524
20	22.365054502317523	23.20944533464563	25.42604628646074	28.99945387657611
21	20.88261947200067	32.42822877970421	21.809929043963937	24.879222704331177
22	25.96966914522682	25.400540522158003	20.683474465229143	27.94631586738603
23	27.573931708566136	25.768623708958227	24.818508983030167	21.838935599445474
24	25.989773688853678	26.98329822539894	22.04558230160016	24.98134578414722
25	24.33452922558096	27.170564551017247	21.398087243122948	27.096818980278847
26	23.972375411080193	32.448368327852265	22.37419681254743	21.20505944852011
27	25.18396917179757	26.58708417264282	22.43711034484163	25.791836310717976
28	25.03091045911515	25.476882257293948	22.194734867202925	27.297472416387976
29	21.84776406965783	30.24842062612665	25.7532000926125	22.15061521160302
30	27.845347891306204	27.77076185938713	24.541197079806015	19.84269316950066
31	25.114389682454032	30.801655048705328	22.43515123955772	21.64880402928292
32	21.266522495912234	31.451869824357825	22.71048051057545	24.57112716915449
33	23.89282675592105	23.90706043684654	30.026587241310814	22.173525565921594
34	25.195064145169184	30.640297655810894	22.999831168803414	21.164807030216508
35	18.612756833095087	25.41162185901521	24.512598384513375	31.463022923376332
36	20.547569638222594	25.827558368235977	28.130211691527464	25.494660302013965
37	20.762842571206523	32.62403848983636	21.912240295518927	24.700878643438188
38	26.335643477498028	25.567374331081673	22.81274673216949	25.28423545925081
39	21.462031422119104	31.597728883227777	24.07498570618089	22.86525398847223
40	19.164185086025036	26.20582554534474	27.20851400168098	27.421475366949245
41	21.026297207467742	31.276001493188453	23.519517142433088	24.17818415691072
42	20.48654261929487	31.023419190457545	23.665809797812997	24.824228392434584
43	21.472894623576718	23.72690006927965	24.709474280216632	30.090731026927
44	22.105962699546115	24.929183811018575	22.651754026271508	30.3130994631638
45	22.090275301244155	24.53764861294584	29.663068647000618	23.709007438809387
46	21.51704984772695	27.272440371078595	26.147355555778702	25.063154225415758
47	21.932680954415332	31.279707741421127	25.009166719928956	21.778444584234588
48	25.28164404327155	27.79749190381038	20.228631227175637	26.69223282574244
49	18.250294013328606	28.70410125259012	22.485952696522244	30.559652037559037
50	20.307260104724364	30.426692945813965	27.112872859916454	22.153174089545214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18914.0
1	15455.5
2	11068.0
3	9629.5
4	8371.0
5	6021.5
6	3373.0
7	2396.5
8	2522.0
9	2541.5
10	2354.5
11	2038.0
12	1828.5
13	1833.5
14	2059.0
15	2127.5
16	1848.0
17	1636.5
18	1744.5
19	1925.0
20	1991.0
21	2184.5
22	2414.0
23	2920.5
24	3695.0
25	4152.5
26	4835.0
27	5951.5
28	6677.5
29	7581.0
30	9550.5
31	13918.5
32	20777.5
33	24062.0
34	27153.5
35	32203.5
36	31489.5
37	30481.0
38	31681.5
39	31343.5
40	33307.0
41	38066.0
42	41890.0
43	45451.0
44	50099.5
45	54791.5
46	62582.5
47	70473.5
48	82824.5
49	98267.5
50	114813.0
51	128178.0
52	119618.0
53	99418.0
54	84507.5
55	78200.0
56	74354.0
57	68735.0
58	66531.5
59	65279.5
60	58546.5
61	50898.0
62	45888.5
63	38048.0
64	30486.5
65	27358.0
66	21620.5
67	14697.5
68	11212.0
69	8819.0
70	6738.0
71	4471.0
72	2845.5
73	2076.0
74	1483.5
75	1029.0
76	773.0
77	507.0
78	282.5
79	214.5
80	146.5
81	108.0
82	95.5
83	79.5
84	58.0
85	38.5
86	23.0
87	17.5
88	14.5
89	12.5
90	13.0
91	14.0
92	12.0
93	11.5
94	7.5
95	2.5
96	1.0
97	0.0
98	2.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5816.0
25	5708.0
26	6292.0
27	6560.0
28	7926.0
29	6166.0
30	6893.0
31	6463.0
32	6521.0
33	5583.0
34	6838.0
35	6203.0
36	5368.0
37	5517.0
38	5934.0
39	7701.0
40	8913.0
41	8257.0
42	12173.0
43	20266.0
44	52312.0
45	99261.0
46	102561.0
47	130126.0
48	143002.0
49	185438.0
50	135976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.331976840979658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.31396376967595	8.424462191231672
2	11.498197286071909	2.835910052897079
3	4.477615364201296	1.6565354692243524
4	2.3587543283073704	1.163524150009881
5	1.4489474397804547	0.8934193135584667
6	1.081357469482192	0.8001165162284857
7	0.8313952448030357	0.7176922833228154
8	0.594912070040579	0.5869153496127753
9	0.5013272867637312	0.556412084110935
>10	5.051698784427996	14.601359628472654
>50	2.158209952261563	19.219055846808118
>100	1.592546716612675	32.480804068569014
>500	0.05681698674169039	4.782559039306885
>1k	0.030079581216189033	5.785335395453273
>5k	0.0025066317680157526	2.281594415757241
>10k+	0.0016710878453438352	3.214304195436352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	18747	1.8751237779738221	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	12448	1.245081388393777	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	9652	0.9654181845096992	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7048	0.7049593208065023	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5443	0.5444230396069512	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3453	0.3453780554405296	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2999	0.299967792721155	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2497	0.24975644495656016	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	2462	0.24625565377775377	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	2295	0.22955187872459176	No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTTCA	1964	0.196444396433594	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1954	0.19544417038250644	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1857	0.18574197768695724	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1754	0.17543964936075554	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	1722	0.1722389259972754	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1649	0.1649372758243363	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1627	0.16273677851194368	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	1579	0.15793569346672348	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	1550	0.1550350379185696	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1530	0.1530345858163945	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	1470	0.14703322950986922	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA	1429	0.1429323027004103	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1407	0.1407318053880177	No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG	1404	0.14043173757269142	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1342	0.13423033605594867	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	1341	0.1341303134508399	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1335	0.13353017782018736	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	1317	0.1317297709282298	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	1243	0.12432809815018193	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	1206	0.12062726176115801	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1136	0.11362567940354519	No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT	1117	0.11172524990647886	No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTT	1106	0.11062500125028256	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	1085	0.10852452654299871	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	1080	0.10802441351745494	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1079	0.1079243909123462	No Hit
AGTTGGCCCTGTGGTGTTCCGAAGCCGGTTACGTACGGCCTGAGGGCCA	1068	0.10682414225614989	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGC	1036	0.10362341889266974	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	1025	0.10252317023647345	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1025	0.10252317023647345	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG	1004	0.10042269552918959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06071372130101403	0.0	0.0	0.0	0.0
2	0.12602848243703077	0.0	0.0	0.0	0.0
3	0.1616365298557474	0.0	0.0	0.0	0.0
4	0.2043461822371856	0.0	0.0	0.0	0.0
5	0.2699610111885286	0.0	0.0	0.0	0.0
6	0.3080696237349641	0.0	0.0	0.0	0.0
7	0.34027690257998305	0.0	0.0	0.0	0.0
8	0.3800858994132674	0.0	0.0	0.0	0.0
9	0.4110929069969813	0.0	0.0	0.0	0.0
10	0.44310014063178277	0.0	0.0	0.0	0.0
11	0.476007577712563	0.0	0.0	0.0	0.0
12	0.5054142236145369	0.0	0.0	0.0	0.0
13	0.5388217737208609	0.0	0.0	0.0	0.0
14	0.5696287360943574	0.0	0.0	0.0	0.0
15	0.6065370773794878	0.0	0.0	0.0	0.0
16	0.6367439041223316	0.0	0.0	0.0	0.0
17	0.6898559074350803	0.0	0.0	0.0	0.0
18	0.7434680237733727	0.0	0.0	0.0	0.0
19	0.7797762294278507	0.0	0.0	0.0	0.0
20	0.8092828979349332	0.0	0.0	0.0	0.0
21	0.8402899055186472	0.0	0.0	0.0	0.0
22	0.8618947882221382	0.0	0.0	0.0	0.0
23	0.8907012984934595	0.0	0.0	0.0	0.0
24	0.919007695739237	0.0	0.0	0.0	0.0
25	0.9548157883681712	0.0	0.0	0.0	0.0
26	1.0036268196612435	0.0	0.0	0.0	0.0
27	1.0301328100150633	0.0	0.0	0.0	0.0
28	1.062140043649865	0.0	0.0	0.0	0.0
29	1.09464739031021	0.0	0.0	0.0	0.0
30	1.126854669155229	0.0	0.0	0.0	0.0
31	1.1530605916937229	0.0	0.0	0.0	0.0
32	1.1817670793599353	0.0	0.0	0.0	0.0
33	1.2052723915604926	0.0	0.0	0.0	0.0
34	1.2341789244369228	0.0	0.0	0.0	0.0
35	1.255683784535305	0.0	0.0	0.0	0.0
36	1.2758883507672734	0.0	0.0	0.0	0.0
37	1.2933923066613056	0.0	0.0	0.0	0.0
38	1.305795109694791	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGT	10	9.097896E-4	287.89102	44
CGTGCGC	10	9.097896E-4	287.89102	44
ATGTACA	15	7.965622E-6	287.89102	44
TGCGATT	20	6.9630914E-8	287.89102	44
GTCGTCA	15	7.965622E-6	287.89102	44
ATATGTA	20	2.5105568E-5	215.91827	44
CGGTCTG	20	2.5105568E-5	215.91827	44
GTCCCCG	35	4.5802153E-9	205.63644	44
AATCGCA	120	0.0	203.92282	44
GGCCGAA	15	0.0030625628	191.92735	44
GCCCGTC	15	0.0030625628	191.92735	44
TAATAAC	25	6.112312E-5	172.73463	44
CTATCAT	1840	0.0	171.32646	44
CCAACAA	60	3.6379788E-12	167.93643	44
AATTCCC	35	1.1329957E-6	164.50916	44
CCTATTA	80	0.0	161.9387	44
ACTTCTA	475	0.0	151.52159	44
ACATTGG	270	0.0	143.94553	44
GTTAACA	65	1.1878001E-9	132.87279	44
GGAATTC	35	2.33512E-4	123.38187	44
>>END_MODULE