FastQCFastQC Report
Tue 24 May 2016
ERR841004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847192
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC358734.23434121190946No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA241992.8563773029018216No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT138241.6317434536681177No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT89051.0511194628844467No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC52780.6229992728920953No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC37530.4429928516794304No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA34500.4072276414319304No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA33540.39589608967034623No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC23010.2716031312854701No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT22630.267117725379843No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA20020.23631006902803614No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT20010.23619203203051964No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC19090.22533262825900152No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18190.21470929848251635No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG15500.18295734615057743No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG15000.17705549627475237No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG14780.17445868232938933No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG14000.1652517965231022No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA13050.15403828175903456No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT13020.15368417076648505No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA12910.15238576379380353No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12530.1479003578881765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA12280.14494943295026394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA12210.14412317396764843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA11890.14034599004712037No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC11530.13609665813652633No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT9920.11709270153636955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9830.11603036855872104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA9230.10894814870773095No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT9140.10788581573008243No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT8630.10186592885674085No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG8600.10151181786419135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAC301.0913936E-11258.2665744
CGTCGTT100.0012593028258.2665744
GGTACCA303.4870027E-9215.2221544
CGTGGCA253.6397614E-7206.6132544
GCGTCGA253.6397614E-7206.6132544
CTATCAT13650.0186.3681644
CTTATGT150.004237843172.1777244
AGCGTCC259.4208954E-5154.9599544
ACTTGAT259.4208954E-5154.9599544
TGGGCAG351.944989E-6147.5808944
AATCGCA1400.0138.3570944
TTTAGAC800.0129.1332944
CCCCTAG601.4479156E-9129.1332944
GATCCAC301.9474886E-4129.1332944
GCCCTTA507.3752744E-8129.1332944
AACTACA301.9474886E-4129.1332944
GAACACA4600.0129.1332944
GAGTCGC301.9226718E-9124.0390843
CGTTCTA152.2886595E-4124.0390843
CGGGCAA204.626814E-6124.0390843
Produced by FastQC (version 0.11.5)