##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841004.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	847192
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.82664732433734	38.0	35.0	39.0	30.0	39.0
2	36.99024778326519	39.0	37.0	39.0	33.0	39.0
3	37.195767901491045	39.0	37.0	39.0	33.0	39.0
4	37.83286315262656	39.0	37.0	40.0	34.0	41.0
5	38.323423733935165	40.0	37.0	41.0	34.0	41.0
6	38.14015240937119	39.0	37.0	40.0	34.0	41.0
7	38.251739865343396	39.0	37.0	41.0	34.0	41.0
8	38.15942785106564	39.0	37.0	40.0	34.0	41.0
9	38.1734506463706	39.0	37.0	40.0	34.0	41.0
10	38.074702074618266	39.0	37.0	40.0	34.0	41.0
11	37.98274535170304	39.0	37.0	40.0	34.0	41.0
12	38.0506036412053	39.0	37.0	40.0	34.0	41.0
13	37.938011690384236	39.0	37.0	40.0	34.0	41.0
14	37.88524325064448	39.0	36.0	40.0	34.0	41.0
15	37.721089198198285	39.0	36.0	40.0	33.0	41.0
16	37.79371618240021	39.0	36.0	40.0	33.0	41.0
17	37.87533050359305	39.0	36.0	40.0	33.0	41.0
18	37.81672159321618	39.0	36.0	40.0	33.0	41.0
19	37.98418068159284	39.0	36.0	40.0	34.0	41.0
20	37.880616200341834	39.0	36.0	40.0	33.0	41.0
21	37.848316556341416	39.0	36.0	40.0	33.0	41.0
22	37.65703760186593	39.0	36.0	40.0	33.0	41.0
23	37.659210662990205	39.0	36.0	40.0	33.0	41.0
24	37.38215894389938	39.0	35.0	40.0	33.0	41.0
25	37.34345888205959	38.0	35.0	40.0	33.0	41.0
26	37.226727380621945	38.0	35.0	40.0	33.0	41.0
27	37.05584922313404	38.0	35.0	40.0	32.0	41.0
28	36.93203370816593	38.0	35.0	40.0	32.0	41.0
29	36.888972415377225	38.0	35.0	40.0	32.0	41.0
30	36.8007549281407	38.0	35.0	40.0	32.0	41.0
31	36.695423715483116	38.0	35.0	40.0	32.0	41.0
32	36.584403927280604	38.0	35.0	40.0	31.0	41.0
33	36.49039932030586	38.0	34.0	40.0	31.0	41.0
34	36.51770573120664	38.0	34.0	40.0	31.0	41.0
35	36.25871031766436	37.0	34.0	40.0	31.0	41.0
36	36.23998959891125	37.0	34.0	40.0	31.0	41.0
37	36.06694075479654	37.0	34.0	39.0	31.0	41.0
38	35.87581146291241	36.0	34.0	39.0	31.0	41.0
39	35.81576509280571	36.0	34.0	39.0	31.0	41.0
40	35.54669940780967	36.0	34.0	39.0	30.0	40.0
41	35.41404677772169	36.0	34.0	39.0	30.0	40.0
42	35.31676909426201	36.0	34.0	39.0	30.0	40.0
43	35.03274167344184	35.0	33.0	39.0	29.0	40.0
44	34.59080254492689	35.0	33.0	37.0	29.0	40.0
45	34.28842343362587	35.0	32.0	37.0	28.0	40.0
46	34.0097801933151	35.0	32.0	37.0	28.0	40.0
47	33.988016707765084	35.0	32.0	37.0	28.0	39.0
48	34.77948353554613	35.0	34.0	37.0	30.0	39.0
49	35.261494581139196	36.0	34.0	37.0	31.0	40.0
50	35.165450158576	36.0	34.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	30.0
22	53.0
23	123.0
24	214.0
25	404.0
26	674.0
27	1263.0
28	2575.0
29	4890.0
30	9163.0
31	17458.0
32	30609.0
33	50016.0
34	71683.0
35	80303.0
36	118191.0
37	146301.0
38	134892.0
39	125416.0
40	52923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.287344545274266	18.810611998224726	38.902043456501005
2	21.651762528446916	21.265781546567954	37.29260899536351	19.78984692962162
3	20.27639543338464	31.622583782660836	23.02653943852161	25.074481345432915
4	18.755606757382033	33.64573792009368	26.996005628004045	20.60264969452025
5	14.218972794832812	34.62615322146574	26.751196895154816	24.403677088546637
6	16.250389522091805	28.302557153514197	25.384682574906282	30.06237074948772
7	20.739808685634426	26.47794124590412	19.681370928903956	33.100879139557506
8	19.887817637560318	26.883398332373297	33.33648098660044	19.892303043465944
9	20.457818298567503	35.501043447058045	24.374639987157575	19.666498267216877
10	32.64962369805192	21.094982011161576	24.395886646710547	21.85950764407596
11	22.256820177716506	23.474961992086797	22.888672225422336	31.37954560477436
12	20.060977912917025	25.899087810083195	23.897652480193393	30.14228179680639
13	21.275932728354373	24.39942775663604	21.711489249190265	32.61315026581932
14	16.64262646483914	25.39636823766041	24.221073853388607	33.73993144411184
15	19.909418408105836	40.73138084401175	20.677603187943227	18.681597559939185
16	16.831485660865543	42.548442383780774	19.51352231843549	21.106549636918196
17	17.206371164977952	44.414489277519145	19.60665350947601	18.77248604802689
18	17.719359956184665	28.95069830687731	19.806844257263997	33.52309747967403
19	20.120350522667824	24.152494357831518	20.37188736437549	35.35526775512516
20	18.74734416755588	25.245162843841772	24.682598513678126	31.32489447492422
21	19.307311683774163	39.706701668571	19.963833463960945	21.0221531836939
22	21.824804766806107	27.904418360891036	19.5557795635464	30.71499730875646
23	24.446760592640157	24.38597153891916	30.808246536794492	20.35902133164619
24	20.813699846079757	27.841504641214744	22.819856655870215	28.524938856835284
25	20.21827089159157	25.805597067229908	21.196058911995515	32.780073129183016
26	20.969405335043405	38.68492711453976	22.10886876788637	18.23679878253046
27	21.286826677984656	28.913569290945528	21.00974488700899	28.789859144060827
28	20.06757693964311	25.061505478915365	21.467054721016986	33.40386286042454
29	19.631549919974255	29.378845442831647	31.040285562323653	19.94931907487044
30	23.53783739952139	28.282693229044803	30.049928258304764	18.12954111312904
31	22.892034041554467	36.33437824975875	20.82729416582087	19.946293542865916
32	18.07057674223035	29.514402504717747	23.379128107450075	29.035892645601823
33	17.93035671261365	24.79450665111148	34.96557146299091	22.309565173283964
34	21.238074339065136	37.757809888570506	21.951253741321285	19.05286203104308
35	16.585982250285372	26.51312203700085	22.65659546898942	34.24430024372435
36	17.437088937070826	26.897939963466822	24.26325621348623	31.401714885976123
37	17.707056272661724	42.20020146648862	18.553788168659207	21.53895409219044
38	30.780969197156015	26.283539157178858	20.08891380547944	22.846577840185684
39	17.85058175137783	38.79508309225669	23.4129854087041	19.94134974766138
40	16.931157041631767	27.121837800839216	25.439548513132188	30.50745664439683
41	18.92175668854114	36.88036345280162	20.944708059902407	23.25317179875484
42	17.657481575981105	38.98286603724466	20.39651678027066	22.963135606503574
43	18.75068154305489	24.39710183737106	21.47105633307061	35.381160286503444
44	18.95456234519845	23.429519286877916	21.796058273908848	35.819860094014786
45	17.307775959488993	25.184982967917286	37.73322553597437	19.774015536619352
46	17.633896927078972	28.569744939527116	33.28528068949851	20.511077443895406
47	18.15228222032107	35.54023451260631	24.946907553721502	21.360575713351114
48	28.722645641256467	23.934020806255027	24.024801622422608	23.318531930065898
49	17.280656822679248	25.315532102282152	21.5800005159065	35.8238105591321
50	15.405393101564515	33.50581951167696	29.08921227939726	21.999575107361267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26113.0
1	21464.5
2	13757.0
3	10195.0
4	8782.0
5	6190.0
6	3406.5
7	2203.5
8	1847.5
9	1545.0
10	1578.5
11	1772.0
12	1875.0
13	1889.0
14	1956.0
15	1984.5
16	1929.5
17	1985.0
18	2341.0
19	2717.0
20	2985.0
21	3612.5
22	4258.5
23	4721.5
24	5121.5
25	5456.0
26	5864.0
27	6681.5
28	7923.5
29	9153.0
30	10775.0
31	15457.5
32	24288.5
33	29001.5
34	30759.0
35	35237.5
36	35986.0
37	33087.5
38	32078.0
39	33996.5
40	36347.5
41	38369.0
42	41693.0
43	46860.0
44	51329.5
45	51193.0
46	50616.5
47	51248.5
48	64168.0
49	83794.5
50	107016.5
51	125917.0
52	105872.0
53	74378.0
54	62651.5
55	58704.5
56	55244.0
57	51764.0
58	48651.5
59	42734.5
60	34939.5
61	28657.0
62	23402.5
63	18149.5
64	13718.0
65	11337.0
66	8797.5
67	6128.0
68	4568.5
69	3330.0
70	2305.0
71	1630.5
72	1226.0
73	1058.5
74	913.5
75	837.0
76	699.0
77	505.0
78	312.0
79	159.0
80	110.5
81	65.5
82	34.0
83	27.5
84	24.5
85	16.5
86	9.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5394.0
25	5966.0
26	6474.0
27	7480.0
28	7765.0
29	6354.0
30	6738.0
31	6680.0
32	5751.0
33	4776.0
34	5886.0
35	4932.0
36	4630.0
37	4930.0
38	5724.0
39	6771.0
40	8066.0
41	9186.0
42	9230.0
43	16698.0
44	41078.0
45	81214.0
46	88694.0
47	101317.0
48	124091.0
49	139569.0
50	131798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.805413726626757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.511	7.8518987237167215
2	11.708	2.7643556782269214
3	4.626	1.6383553169812615
4	2.461	1.1621249272491379
5	1.477	0.871829803711386
6	1.127	0.7982820761945012
7	0.889	0.7346508962079831
8	0.736	0.6951027602237835
9	0.617	0.6555546242395838
>10	6.013	16.95198384074969
>50	2.299	19.351198072412046
>100	1.464	27.924997845511996
>500	0.046	3.6302827750749938
>1k	0.021	4.571292303224412
>5k	0.002	1.6743618288474729
>10k+	0.003	8.723728527428108
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	35873	4.23434121190946	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	24199	2.8563773029018216	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	13824	1.6317434536681177	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8905	1.0511194628844467	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5278	0.6229992728920953	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3753	0.4429928516794304	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3450	0.4072276414319304	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3354	0.39589608967034623	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2301	0.2716031312854701	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	2263	0.267117725379843	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2002	0.23631006902803614	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2001	0.23619203203051964	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	1909	0.22533262825900152	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1819	0.21470929848251635	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	1550	0.18295734615057743	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1500	0.17705549627475237	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	1478	0.17445868232938933	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1400	0.1652517965231022	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1305	0.15403828175903456	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1302	0.15368417076648505	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1291	0.15238576379380353	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.1479003578881765	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	1228	0.14494943295026394	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	1221	0.14412317396764843	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	1189	0.14034599004712037	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1153	0.13609665813652633	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	992	0.11709270153636955	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	983	0.11603036855872104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	923	0.10894814870773095	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	914	0.10788581573008243	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	863	0.10186592885674085	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	860	0.10151181786419135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0529986118849092	0.0	0.0	0.0	0.0
2	0.09997733689647684	0.0	0.0	0.0	0.0
3	0.13704095411665831	0.0	0.0	0.0	0.0
4	0.17079953540637777	0.0	0.0	0.0	0.0
5	0.19358067592706257	0.0	0.0	0.0	0.0
6	0.22863766418946355	0.0	0.0	0.0	0.0
7	0.25366150766296186	0.0	0.0	0.0	0.0
8	0.2953285677862869	0.0	0.0	0.0	0.0
9	0.32613622413809384	0.0	0.0	0.0	0.0
10	0.3524584745842737	0.0	0.0	0.0	0.0
11	0.38078735398823405	0.0	0.0	0.0	0.0
12	0.41230323232514	0.0	0.0	0.0	0.0
13	0.4421665926968149	0.0	0.0	0.0	0.0
14	0.4722660270635228	0.0	0.0	0.0	0.0
15	0.5051983493706267	0.0	0.0	0.0	0.0
16	0.5341174137621696	0.0	0.0	0.0	0.0
17	0.565751329096592	0.0	0.0	0.0	0.0
18	0.6030510203118066	0.0	0.0	0.0	0.0
19	0.6264223458200738	0.0	0.0	0.0	0.0
20	0.6541610402364517	0.0	0.0	0.0	0.0
21	0.681781697655313	0.0	0.0	0.0	0.0
22	0.7052710601610969	0.0	0.0	0.0	0.0
23	0.7300588296395623	0.0	0.0	0.0	0.0
24	0.7561450060907091	0.0	0.0	0.0	0.0
25	0.781995108546823	0.0	0.0	0.0	0.0
26	0.8050123230625407	0.0	0.0	0.0	0.0
27	0.826022908620478	0.0	0.0	0.0	0.0
28	0.8519910480741083	0.0	0.0	0.0	0.0
29	0.8811461864606842	0.0	0.0	0.0	0.0
30	0.9105373988422931	0.0	0.0	0.0	0.0
31	0.9334365763604945	0.0	0.0	0.0	0.0
32	0.9553914578985637	0.0	0.0	0.0	0.0
33	0.980651375367095	0.0	0.0	0.0	0.0
34	1.006737551818242	0.0	0.0	0.0	0.0
35	1.0264497304034976	0.0	0.0	0.0	0.0
36	1.041794540080643	0.0	0.0	0.0	0.0
37	1.0613886816683822	0.0	0.0	0.0	0.0
38	1.0720120114448672	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTAC	30	1.0913936E-11	258.26657	44
CGTCGTT	10	0.0012593028	258.26657	44
GGTACCA	30	3.4870027E-9	215.22215	44
CGTGGCA	25	3.6397614E-7	206.61325	44
GCGTCGA	25	3.6397614E-7	206.61325	44
CTATCAT	1365	0.0	186.36816	44
CTTATGT	15	0.004237843	172.17772	44
AGCGTCC	25	9.4208954E-5	154.95995	44
ACTTGAT	25	9.4208954E-5	154.95995	44
TGGGCAG	35	1.944989E-6	147.58089	44
AATCGCA	140	0.0	138.35709	44
TTTAGAC	80	0.0	129.13329	44
CCCCTAG	60	1.4479156E-9	129.13329	44
GATCCAC	30	1.9474886E-4	129.13329	44
GCCCTTA	50	7.3752744E-8	129.13329	44
AACTACA	30	1.9474886E-4	129.13329	44
GAACACA	460	0.0	129.13329	44
GAGTCGC	30	1.9226718E-9	124.03908	43
CGTTCTA	15	2.2886595E-4	124.03908	43
CGGGCAA	20	4.626814E-6	124.03908	43
>>END_MODULE