FastQCFastQC Report
Tue 24 May 2016
ERR841003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856232
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC102961.2024778331106523No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA64780.7565706490764186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA62790.7333292845864205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA62540.7304095151781294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA55500.6481888086406488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA51930.6064945014902503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44760.5227555148604583No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT41350.4829298601313663No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT41010.4789589737360902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35980.4202132132412711No Hit
AAAAAAAAAAAAAAAAAAAAAAAA33750.39416887011931345No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC24900.2908090330658046No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA22840.266750133141485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21160.2471292827177681No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA16580.1936391071578731No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA15960.1863980790253109No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA15530.18137607564305003No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA14600.1705145334442067No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA14460.16887946257556363No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC13430.15685001261340384No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13340.15579889562641902No Hit
AGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA13180.15393024320511264No Hit
AGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAA13040.15229517233646955No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA12300.14365265488792756No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA11990.14003214082164647No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC11950.13956497771631987No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA10810.12625082921451197No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCCC10690.12484933989853218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10670.12461575834586888No Hit
AGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA10580.12356464135888405No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC10530.1229806874772258No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10170.11877621952928645No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.11013370208074447No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC9390.10966653897541787No Hit
AGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA8610.1005568584215493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCA256.5774657E-9193.5974944
ACGCGAG100.002984808193.5974744
ACCGCTA153.8810977E-5193.5974744
TACGGAT100.002984808193.5974744
GGATTGC100.002984808193.5974744
GAACAAC251.5310379E-6154.8779944
AAGGCTC201.2215691E-4145.198144
AATCGCA1100.0140.7981744
TAGTCTG354.908361E-8138.283944
GCCGAAT303.7933842E-6129.0649944
ACGGCCG554.1836756E-11123.19839544
GATAACC152.3547003E-4123.1557243
TAACGAA204.7942012E-6123.1557243
GCCGATG152.3547003E-4123.1557243
GTTCGGC500.0123.1557243
GATGCGA259.814539E-8123.1557243
GACGAAC750.0123.1557243
ACTCAAC152.3547003E-4123.1557243
CCAGTAC152.3547003E-4123.1557243
TAGGAAC401.08884706E-7120.9984244
Produced by FastQC (version 0.11.5)