FastQCFastQC Report
Tue 24 May 2016
ERR841002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412214
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC150670.6246129074783581No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA96120.39847210902515284No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT56950.23609016447131143No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT51250.21246042017830924No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA46440.19252023245035474No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA44510.18451928394412767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA43040.17842529725803763No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG42790.1773889049644849No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA40820.1692221336912894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA40110.16627877957759968No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA38610.1600604258162833No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG38460.15943859044015166No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT36590.15168637608437727No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA34700.14385125034511864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34410.14264903528459746No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT32180.13340441602610714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA30400.12602530289601171No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC29880.12386960692542205No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29310.12150663249612181No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC28290.11727815193842668No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.11698796209623193No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.11520536735132123No Hit
AAAAAAAAAAAAAAAAAAAAAAAA27330.11329840553118423No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA26760.11093543110188399No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC25670.1064167607019941No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.10272720413694639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG251.690794E-4133.8534544
CTATCAT10350.0117.4720444
ACCGTCA251.3078534E-7117.4044943
CGCGTAA302.8267095E-9117.4044943
TCCAACG206.0887305E-6117.4044843
TCGCGTT801.8189894E-12111.5445444
CGTGCGC802.6557245E-1097.6014844
TACGCCG605.25466E-792.9537944
GATTAGC802.9729563E-883.6584144
TCGTGCG2050.083.0421943
ACGCGTA351.2241799E-980.5309642
CCGTCAA701.314902E-679.67467544
AACGACA4250.078.73006443
CGCCAAT304.558541E-578.2696643
GTTAAAC1600.076.68687444
TTATCGC700.074.7787542
GACACCC605.8722246E-574.3630344
AGCGCCT1550.071.9642244
GTCGCCC250.002169738570.44269643
CGATCTA250.002169738570.44269643
Produced by FastQC (version 0.11.5)