##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2412214
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.30964665655701	39.0	35.0	39.0	31.0	39.0
2	37.11752522786121	39.0	37.0	39.0	33.0	39.0
3	37.319938032031985	39.0	37.0	39.0	34.0	39.0
4	37.89089152123319	39.0	37.0	40.0	34.0	41.0
5	38.5496485801011	40.0	37.0	41.0	35.0	41.0
6	38.482673593636385	40.0	37.0	41.0	35.0	41.0
7	38.53021456636932	40.0	37.0	41.0	35.0	41.0
8	38.49962150953439	40.0	37.0	41.0	35.0	41.0
9	38.52965284174621	40.0	37.0	41.0	35.0	41.0
10	38.49628391179224	40.0	37.0	41.0	35.0	41.0
11	38.457288615355026	40.0	37.0	41.0	35.0	41.0
12	38.370841890479035	40.0	36.0	41.0	34.0	41.0
13	38.27287670165251	40.0	36.0	41.0	34.0	41.0
14	38.199355032347874	40.0	36.0	41.0	34.0	41.0
15	38.07420195720612	40.0	36.0	41.0	34.0	41.0
16	38.00360457239697	40.0	35.0	41.0	34.0	41.0
17	38.00508205325067	40.0	35.0	41.0	34.0	41.0
18	38.00669550877327	40.0	35.0	41.0	34.0	41.0
19	37.95639690342565	40.0	35.0	41.0	34.0	41.0
20	37.906221421482506	39.0	35.0	41.0	34.0	41.0
21	37.797534132543795	39.0	35.0	41.0	34.0	41.0
22	37.71847978661926	39.0	35.0	41.0	34.0	41.0
23	37.63471814689741	39.0	35.0	41.0	33.0	41.0
24	37.48554398573261	39.0	35.0	41.0	33.0	41.0
25	37.37160894230456	38.0	35.0	41.0	33.0	41.0
26	37.24656569140523	38.0	35.0	40.0	33.0	41.0
27	37.12877521483913	38.0	35.0	40.0	33.0	41.0
28	37.019890945822134	38.0	35.0	40.0	33.0	41.0
29	36.92903528082378	38.0	35.0	40.0	32.0	41.0
30	36.88198395306539	38.0	35.0	40.0	32.0	41.0
31	36.85461608893213	38.0	35.0	40.0	32.0	41.0
32	36.69844480446385	38.0	35.0	40.0	32.0	41.0
33	36.613884344003594	38.0	35.0	40.0	32.0	41.0
34	36.55060101126795	37.0	35.0	40.0	32.0	41.0
35	36.45014382477863	37.0	35.0	40.0	31.0	41.0
36	36.41859049766363	37.0	34.0	40.0	32.0	41.0
37	36.316235545569135	37.0	34.0	40.0	31.0	41.0
38	36.169383092015394	36.0	34.0	40.0	31.0	41.0
39	36.00342025241019	36.0	34.0	40.0	31.0	41.0
40	35.81678865827887	36.0	34.0	40.0	31.0	41.0
41	35.699544463877174	36.0	34.0	39.0	30.0	41.0
42	35.560354654483426	35.0	34.0	39.0	30.0	41.0
43	35.453310732058874	35.0	34.0	39.0	30.0	41.0
44	35.19407399033712	35.0	33.0	39.0	29.0	41.0
45	34.97922438032729	35.0	33.0	39.0	29.0	41.0
46	34.69372153022151	35.0	33.0	39.0	28.0	41.0
47	34.684947136386874	35.0	33.0	38.0	29.0	40.0
48	35.559216216787505	36.0	34.0	39.0	31.0	40.0
49	36.09798834492787	36.0	34.0	39.0	31.0	41.0
50	36.368323342526274	37.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	20.0
22	77.0
23	135.0
24	403.0
25	875.0
26	1691.0
27	3336.0
28	6803.0
29	13154.0
30	24695.0
31	46538.0
32	87424.0
33	152242.0
34	250811.0
35	165048.0
36	253945.0
37	377568.0
38	357250.0
39	335026.0
40	335171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.04230802076433	19.89607887194088	34.06161310729479
2	24.28868251324302	23.22534402005792	31.62932476140177	20.85664870529729
3	23.541941137892408	21.681078046972615	28.055636854773248	26.721343960361725
4	18.507644844114164	23.993144886813525	33.167952760410145	24.331257508662166
5	18.55399230748184	24.108350254164847	32.06174908196371	25.27590835638961
6	20.155467135171257	26.630265805604314	31.234915310167338	21.97935174905709
7	25.05221344374919	27.08470309848131	24.20411290208912	23.658970555680384
8	25.177990012494746	24.399120476044	28.1431083643491	22.279781147112153
9	25.48024346098646	26.2526044538337	26.48434177067209	21.78281031450775
10	27.145808788109182	21.954727068162278	26.72934490886795	24.170119234860586
11	25.362384929363646	25.649548506061237	25.584504525717865	23.40356203885725
12	23.82305218359565	25.474646942601275	26.563605053283002	24.13869582052007
13	23.524985759969887	24.419060663771955	26.814453444014504	25.241500132243655
14	21.704832158340846	26.558506003198723	25.612072560726368	26.124589277734067
15	21.571386286622996	29.698567374204778	25.99864688622154	22.731399452950694
16	22.153424198682206	32.74013002163158	22.495558022629833	22.610887757056382
17	22.953353226537946	31.829016828523503	22.750344704076834	22.467285240861713
18	23.65814144184554	27.303920796413582	22.87724057649943	26.160697185241442
19	23.574732590060417	26.67798130679948	24.55404039608426	25.19324570705584
20	24.022039503957775	26.146892439891317	26.297542423682145	23.53352563246876
21	23.82599553770934	30.48954197264422	24.130487593555134	21.553974896091308
22	24.83569036578015	29.319164883380992	22.86579880557861	22.979345945260246
23	25.736149446110502	27.23203662693277	24.893603967143875	22.13820995981285
24	25.019753637115116	28.700811785355697	23.611047776026506	22.668386801502685
25	24.03934807585923	27.573440758024937	25.304530811033615	23.082680355082218
26	24.781972500324535	30.729326615466245	24.552801536264678	19.93589934794454
27	25.472527556258623	29.10543700359261	23.6895564541903	21.732478985958466
28	25.390061656766232	27.59300386539511	23.60926465276764	23.40766982507102
29	23.680887859965345	30.075644828453413	24.285279962517787	21.95818734906346
30	25.540972915424693	29.313856557129704	26.19708317286254	18.94808735458307
31	24.069379814241596	29.36451463599506	24.49622742552827	22.069878124235075
32	22.066339706265367	29.08118535652957	26.93322326745567	21.919251669749393
33	22.434908516168246	26.922086006682576	29.990339677582845	20.652665799566332
34	22.881773354898453	28.918215540139958	25.716628247081815	22.48338285787978
35	21.43564139898964	27.433037717309794	25.921693845093113	25.20962703860745
36	23.07657745972617	28.657775018425934	24.879732197642205	23.38591532420569
37	21.693018030361156	31.282219908295428	24.41807785754736	22.606684203796057
38	24.64999212019935	28.2242972619186	23.57622569954481	23.54948491833724
39	23.086750038403807	30.99039257020494	23.99511577591424	21.92774161547701
40	21.155724742138187	30.055094491017403	24.797885789726582	23.991294977117825
41	22.48033790031338	31.369015347671898	24.170969946674344	21.979676805340382
42	21.25677496303701	33.51883388601465	22.86840414518121	22.35598700576713
43	22.493547708443558	29.525233623263205	25.42393035906082	22.55728830923242
44	21.725605650278435	30.102723398817453	24.248079465641727	23.92359148526239
45	21.911086994337463	28.065883794653175	27.936484034032343	22.086545176977022
46	21.326670456295066	29.19262767018056	25.578645229312524	23.90205664421185
47	22.335032413687042	31.55086919113338	24.293500775185205	21.820597619994373
48	24.686211925849204	29.201124521761184	23.265847935910713	22.846815616478892
49	19.366519526631606	29.65903292798786	24.032995936661834	26.941451608718697
50	19.929383247670042	31.815835993526797	25.39867873814589	22.856102020657268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55573.0
1	50404.5
2	41518.5
3	36176.5
4	31760.5
5	21571.5
6	10793.0
7	7772.5
8	8184.0
9	7907.5
10	6952.0
11	5763.5
12	4821.0
13	4294.5
14	4246.5
15	4678.5
16	5312.5
17	5820.0
18	6596.0
19	8074.5
20	9745.5
21	11553.0
22	12866.5
23	14750.5
24	17902.0
25	20713.5
26	24436.0
27	29092.0
28	32774.0
29	37559.5
30	43899.5
31	50608.0
32	57650.5
33	63731.5
34	77251.5
35	92188.5
36	95893.0
37	97570.5
38	101853.5
39	112544.5
40	127030.5
41	139976.5
42	149747.0
43	146087.0
44	139772.5
45	142073.0
46	154306.0
47	166631.5
48	179343.0
49	197259.0
50	216309.0
51	226517.5
52	218899.0
53	207089.5
54	196055.0
55	185932.5
56	176388.5
57	164683.0
58	152389.5
59	137085.5
60	118553.5
61	101560.0
62	87027.0
63	69787.5
64	51056.5
65	40184.5
66	31103.0
67	21480.5
68	14839.0
69	10881.0
70	9010.0
71	7242.5
72	5534.5
73	3884.5
74	2665.0
75	1857.0
76	1195.5
77	709.5
78	374.0
79	245.0
80	178.5
81	108.5
82	51.5
83	35.5
84	27.5
85	16.0
86	6.0
87	2.5
88	1.0
89	1.0
90	2.0
91	3.5
92	4.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	15248.0
25	16647.0
26	17970.0
27	20027.0
28	20154.0
29	19049.0
30	19400.0
31	18409.0
32	17973.0
33	14101.0
34	15607.0
35	14213.0
36	13776.0
37	13189.0
38	15207.0
39	17654.0
40	20597.0
41	20214.0
42	25610.0
43	42579.0
44	108775.0
45	217644.0
46	264645.0
47	260801.0
48	373292.0
49	381206.0
50	428227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.566006838713667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62600532919858	9.00052872796451
2	10.90691879501689	2.741128323349537
3	3.9925663825614537	1.5051184940185653
4	1.8699905391715892	0.9399325561424019
5	1.1642585898877744	0.7315040701280451
6	0.7805470667602291	0.5885015867308159
7	0.5540291477430914	0.48733538415704736
8	0.46617217088762836	0.4686338149913547
9	0.34894860561010493	0.39464015080105597
>10	4.260830300706528	13.30230715582652
>50	2.2848963030446763	20.861299639050326
>100	1.6543834583420371	34.163819656683856
>500	0.05434799782745463	4.638481570905925
>1k	0.034585089526562036	8.481412809681595
>5k	0.001140167786589957	0.9757884731711698
>10k+	3.80055928863319E-4	0.7195675863973187
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	15067	0.6246129074783581	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	9612	0.39847210902515284	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5695	0.23609016447131143	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	5125	0.21246042017830924	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	4644	0.19252023245035474	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	4451	0.18451928394412767	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4304	0.17842529725803763	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4279	0.1773889049644849	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	4082	0.1692221336912894	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	4011	0.16627877957759968	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3861	0.1600604258162833	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	3846	0.15943859044015166	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3659	0.15168637608437727	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3470	0.14385125034511864	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3441	0.14264903528459746	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	3218	0.13340441602610714	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3040	0.12602530289601171	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	2988	0.12386960692542205	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2931	0.12150663249612181	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	2829	0.11727815193842668	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2822	0.11698796209623193	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.11520536735132123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2733	0.11329840553118423	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	2676	0.11093543110188399	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	2567	0.1064167607019941	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2478	0.10272720413694639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08796897787675555	0.0	0.0	0.0	0.0
2	0.14882593335417174	0.0	0.0	0.0	0.0
3	0.18153447413869583	0.0	0.0	0.0	0.0
4	0.2179325714882676	0.0	0.0	0.0	0.0
5	0.25171896025808654	0.0	0.0	0.0	0.0
6	0.2973202211744066	0.0	0.0	0.0	0.0
7	0.33434015390011	0.0	0.0	0.0	0.0
8	0.3669243276094078	0.0	0.0	0.0	0.0
9	0.4034467920342059	0.0	0.0	0.0	0.0
10	0.4346629279160141	0.0	0.0	0.0	0.0
11	0.4647183044290432	0.0	0.0	0.0	0.0
12	0.500743300552936	0.0	0.0	0.0	0.0
13	0.5316277909008074	0.0	0.0	0.0	0.0
14	0.5654141796706262	0.0	0.0	0.0	0.0
15	0.5955524675671395	0.0	0.0	0.0	0.0
16	0.6292974006452163	0.0	0.0	0.0	0.0
17	0.666027143528725	0.0	0.0	0.0	0.0
18	0.6979480261701491	0.0	0.0	0.0	0.0
19	0.7314856807895154	0.0	0.0	0.0	0.0
20	0.7629505508217762	0.0	0.0	0.0	0.0
21	0.7942910537788107	0.0	0.0	0.0	0.0
22	0.8221078229377659	0.0	0.0	0.0	0.0
23	0.8512511742324685	0.0	0.0	0.0	0.0
24	0.8821356645803399	0.0	0.0	0.0	0.0
25	0.9110717374163321	0.0	0.0	0.0	0.0
26	0.9469309107732564	0.0	0.0	0.0	0.0
27	0.9734625534882063	0.0	0.0	0.0	0.0
28	1.0034350186177512	0.0	0.0	0.0	0.0
29	1.0380919769141543	0.0	0.0	0.0	0.0
30	1.0725831124435892	0.0	0.0	0.0	0.0
31	1.1059963999877291	0.0	0.0	0.0	0.0
32	1.140155889983227	0.0	0.0	0.0	0.0
33	1.1734448104521407	0.0	0.0	0.0	0.0
34	1.2026710731303276	0.0	0.0	0.0	0.0
35	1.2333068293277463	0.0	0.0	0.0	0.0
36	1.2593410037417907	0.0	0.0	0.0	0.0
37	1.2812296089816244	0.0	0.0	0.0	0.0
38	1.2975631515280153	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	1.690794E-4	133.85345	44
CTATCAT	1035	0.0	117.47204	44
ACCGTCA	25	1.3078534E-7	117.40449	43
CGCGTAA	30	2.8267095E-9	117.40449	43
TCCAACG	20	6.0887305E-6	117.40448	43
TCGCGTT	80	1.8189894E-12	111.54454	44
CGTGCGC	80	2.6557245E-10	97.60148	44
TACGCCG	60	5.25466E-7	92.95379	44
GATTAGC	80	2.9729563E-8	83.65841	44
TCGTGCG	205	0.0	83.04219	43
ACGCGTA	35	1.2241799E-9	80.53096	42
CCGTCAA	70	1.314902E-6	79.674675	44
AACGACA	425	0.0	78.730064	43
CGCCAAT	30	4.558541E-5	78.26966	43
GTTAAAC	160	0.0	76.686874	44
TTATCGC	70	0.0	74.77875	42
GACACCC	60	5.8722246E-5	74.36303	44
AGCGCCT	155	0.0	71.96422	44
GTCGCCC	25	0.0021697385	70.442696	43
CGATCTA	25	0.0021697385	70.442696	43
>>END_MODULE