FastQCFastQC Report
Tue 24 May 2016
ERR841001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4175246
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC914722.190817020122886No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA601181.4398672557257706No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT368340.8821995159087633No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT340740.8160956264612912No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC212560.5090957514838647No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA132020.31619693785707476No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT117500.28142054384340465No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA111810.26779260431600915No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC87620.20985589831114143No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT77060.18456397539210864No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC76520.1832706384246581No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC70680.16928343862852632No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC64560.15462561966408686No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC63310.1516317840912847No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG58450.1399917513842298No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT58170.13932113221592213No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC55400.1326867925865925No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT51150.12250775163906509No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC50920.12195688589366951No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT48900.11711884760802119No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC48440.11601711611723No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG48430.11599316543264757No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGT47970.11489143394185636No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA46350.11101142303950473No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG44290.10607758201552674No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT44160.10576622311595534No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT43540.10428128067184543No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42870.10267658480482347No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT42270.10123954372987844No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT41960.10049707250782348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT55300.0164.9817844
GCCGTTA206.710041E-6115.13821443
AATCGCA6600.0112.70093544
ACATTGG11750.0103.858744
TGTTTTT28000.097.9592644
GACACGA656.63078E-789.4021844
TATACCG656.63078E-789.4021844
ACCACTC6550.086.9453344
CCTATCA56200.081.5391643
ACTTCTA34900.080.5900544
CCTTAGC146700.076.7588243
ACCTTAG110100.073.1467142
CCCATAC17600.072.63927544
CAGGCAC21750.072.1383244
CCCTATC69400.072.1354542
GTCGCAA900.071.6579142
TAATTCC9000.071.0250744
GATGCGC700.069.09869442
CTAATTC4600.068.83263443
TCGAACA550.003295244663.39427644
Produced by FastQC (version 0.11.5)