##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4175246
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.363862153271924	39.0	37.0	39.0	34.0	39.0
2	37.884456388916966	39.0	38.0	39.0	35.0	39.0
3	38.084474543535876	39.0	38.0	39.0	35.0	39.0
4	38.73507189756005	39.0	38.0	41.0	35.0	41.0
5	39.32844268337722	40.0	39.0	41.0	35.0	41.0
6	39.27359178357395	40.0	39.0	41.0	35.0	41.0
7	39.30355600604132	40.0	39.0	41.0	36.0	41.0
8	39.28609811254235	40.0	39.0	41.0	35.0	41.0
9	39.29488777427725	40.0	39.0	41.0	35.0	41.0
10	39.2552893889366	40.0	39.0	41.0	35.0	41.0
11	39.182481463367665	40.0	39.0	41.0	35.0	41.0
12	39.14346986979929	40.0	39.0	41.0	35.0	41.0
13	39.00263433579722	40.0	38.0	41.0	35.0	41.0
14	38.96147221026018	40.0	38.0	41.0	35.0	41.0
15	38.94380067665474	40.0	38.0	41.0	35.0	41.0
16	38.87313585834224	40.0	38.0	41.0	35.0	41.0
17	38.85905596939677	40.0	38.0	41.0	35.0	41.0
18	38.802635341725974	40.0	38.0	41.0	35.0	41.0
19	38.78366568101616	40.0	38.0	41.0	35.0	41.0
20	38.66617056815335	40.0	38.0	41.0	35.0	41.0
21	38.59962694413694	40.0	38.0	41.0	35.0	41.0
22	38.484527378746066	40.0	38.0	41.0	35.0	41.0
23	38.368092802196564	40.0	37.0	41.0	34.0	41.0
24	38.20130430638099	40.0	37.0	41.0	34.0	41.0
25	38.10783404238628	40.0	37.0	41.0	34.0	41.0
26	38.02093503805832	39.0	37.0	41.0	34.0	41.0
27	37.91943369187836	39.0	36.0	41.0	33.0	41.0
28	37.76851368188127	39.0	36.0	41.0	33.0	41.0
29	37.71049547280915	39.0	36.0	41.0	33.0	41.0
30	37.60007587606876	39.0	36.0	41.0	33.0	41.0
31	37.47754144724302	39.0	35.0	41.0	33.0	41.0
32	37.41067626848215	39.0	35.0	41.0	33.0	41.0
33	37.28039967190433	38.0	35.0	40.0	32.0	41.0
34	37.2186200850885	38.0	35.0	40.0	32.0	41.0
35	37.030721306673875	38.0	35.0	40.0	32.0	41.0
36	36.962131444619274	38.0	35.0	40.0	32.0	41.0
37	36.930412183345524	38.0	35.0	40.0	32.0	41.0
38	36.71337103808708	38.0	35.0	40.0	31.0	41.0
39	36.609329266260396	38.0	35.0	40.0	31.0	41.0
40	36.38297161380478	37.0	34.0	40.0	31.0	41.0
41	36.351664638938146	37.0	34.0	40.0	31.0	41.0
42	36.19707117821276	37.0	34.0	40.0	31.0	41.0
43	36.01006970644735	37.0	34.0	40.0	30.0	41.0
44	35.782491712843544	36.0	34.0	40.0	30.0	41.0
45	35.557215454306274	36.0	33.0	40.0	29.0	41.0
46	35.18937834663168	36.0	33.0	40.0	28.0	41.0
47	35.24204831152312	36.0	33.0	39.0	29.0	41.0
48	36.23756501468303	37.0	34.0	40.0	31.0	41.0
49	36.785237675370595	38.0	35.0	40.0	32.0	41.0
50	36.93420540001177	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	46.0
22	91.0
23	184.0
24	394.0
25	683.0
26	1244.0
27	2712.0
28	5679.0
29	11942.0
30	23786.0
31	46767.0
32	86463.0
33	148214.0
34	232542.0
35	256464.0
36	435633.0
37	701379.0
38	720332.0
39	661016.0
40	839662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.68239524090317	19.08606103688262	38.23154372221421
2	22.96470675021304	18.532776272344194	37.59071920552705	20.91179777191571
3	20.632724395161386	23.43402041460551	30.160306722047036	25.77294846818607
4	20.261752241664325	25.696617636421905	33.29461305992509	20.747017061988682
5	18.117950415376725	24.3067833607888	32.55463270906672	25.020633514767752
6	18.412232476840888	23.59343617118608	29.632002521528072	28.36232883044496
7	23.500339860214225	21.523881467103976	23.63247578705542	31.34330288562638
8	22.971939856956933	23.367389610097224	30.595251154063735	23.06541937888211
9	23.465587416885135	26.897313355907652	27.746820187361415	21.890279039845794
10	28.147251682894854	20.29133133712361	26.140663328579922	25.42075365140162
11	24.27090523528434	19.493246625468295	27.83893452026539	28.396913618981973
12	23.035409171100337	22.433001552483375	25.749979761671526	28.781609514744762
13	25.897731534860462	20.866004062994133	25.420729700717036	27.81553470142837
14	20.764692667210507	21.35392740930714	24.260319032698913	33.62106089078344
15	23.18155624842225	32.17573287897288	23.23558899284018	21.40712187976469
16	21.641790687303214	30.814328065939105	23.12033829862959	24.423542948128084
17	22.011804813416983	33.528084333234496	21.011264964986495	23.448845888362026
18	21.616235306853778	24.369031190018504	22.74594119723724	31.26879230589048
19	25.044608150034755	22.0595864291589	22.89366901974159	30.00213640106475
20	22.992992508704877	21.60986442475485	26.45817755408903	28.938965512451244
21	24.776049123812104	29.319134728827954	22.750060714985416	23.154755432374525
22	26.59929498764863	23.01402120976824	22.263550459062774	28.123133343520358
23	27.212264858166442	21.87507035513596	28.624516974568685	22.288147812128916
24	24.967343241571875	24.52229162066139	24.331500467277856	26.178864670488878
25	24.73898116323186	24.688013094798922	23.352976975572144	27.220028766397075
26	26.44930366907392	31.003119502563063	23.222794210307736	19.324782618055288
27	26.246396099393447	26.22196864819361	21.0451007632362	26.486534489176744
28	25.513606000004895	23.1504431710535	22.446509829071335	28.88944099987027
29	24.402943997793354	26.68155001951747	27.10315197889897	21.812354003790208
30	26.234683540790698	26.987521831096206	27.12946112627641	19.64833350183669
31	25.10443517684124	30.127552624251912	23.332579883180298	21.435432315726548
32	21.889788577727316	27.306079467128374	24.4788028973212	26.32532905782311
33	22.81574656935724	23.666479221956113	31.768540844067577	21.74923336461907
34	24.345184976835775	30.07844835676035	24.708447637050654	20.86791902935322
35	21.6241971030166	24.6585639613954	24.436487161841093	29.280751773746903
36	22.762667131420127	24.369237686589965	26.49316443738835	26.37493074460156
37	22.60060586904195	34.35499943084715	21.424650515517648	21.619744184593255
38	28.756293287742064	25.293508588908587	21.00748923730137	24.94270888604798
39	22.213529727152174	32.02547003114339	23.37048303358222	22.390517208122212
40	19.707226468579876	27.55792856937034	26.6653166677197	26.069528294330084
41	21.557748677533585	31.403152593590956	22.37119481015236	24.6679039187231
42	20.394269850871844	32.63516226896002	22.228952590087335	24.741615290080798
43	21.628964543992097	25.64487848294224	23.15431330744118	29.571843665624485
44	21.405087966019952	25.877698127031923	22.308833818975167	30.40838008797295
45	22.098404677021048	24.840001950608148	31.453381195261425	21.60821217710938
46	21.070022645981926	27.68362161103939	27.200211909246757	24.046143833731932
47	20.28069351172512	31.87657182068658	26.966521789023172	20.876212878565127
48	26.400604785505216	23.99202817829594	24.29215346769902	25.31521356849982
49	19.046706434914253	26.846876161180667	23.781048980022348	30.325368423882733
50	18.271917752545708	27.427496727647803	30.438901610810117	23.861683908996376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46890.0
1	41539.0
2	42420.0
3	46984.0
4	41720.5
5	28454.5
6	14847.5
7	11742.5
8	13028.0
9	13183.5
10	12049.0
11	10382.5
12	9162.0
13	8261.0
14	7753.0
15	7998.0
16	8419.0
17	8782.0
18	9269.5
19	10592.5
20	12455.0
21	14718.5
22	17018.0
23	19284.5
24	21995.5
25	26854.5
26	32502.0
27	36252.0
28	38014.5
29	40169.0
30	47671.0
31	76484.0
32	119808.0
33	127488.5
34	118508.5
35	127585.5
36	130023.5
37	129731.5
38	134918.0
39	140533.5
40	146501.0
41	155967.0
42	168807.5
43	180521.5
44	196666.5
45	220077.0
46	250491.5
47	266198.5
48	304327.5
49	366673.5
50	441428.5
51	502868.5
52	461639.0
53	379666.0
54	344352.5
55	334936.0
56	326649.0
57	318743.0
58	304958.0
59	275795.5
60	237874.5
61	209554.0
62	186765.5
63	158014.0
64	131376.0
65	113133.5
66	89627.0
67	67824.0
68	52482.0
69	40389.5
70	33180.0
71	25600.5
72	19066.0
73	13592.5
74	8633.5
75	5531.0
76	4191.5
77	2941.5
78	1562.5
79	939.5
80	654.5
81	472.5
82	336.0
83	308.0
84	315.5
85	256.0
86	207.0
87	105.5
88	15.0
89	9.5
90	5.5
91	3.0
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	19703.0
25	21230.0
26	24183.0
27	24800.0
28	24865.0
29	24937.0
30	27300.0
31	26481.0
32	26803.0
33	22803.0
34	26285.0
35	27097.0
36	22150.0
37	21372.0
38	24798.0
39	27720.0
40	31395.0
41	33515.0
42	41535.0
43	70393.0
44	181308.0
45	400637.0
46	435775.0
47	515360.0
48	622230.0
49	737017.0
50	713554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.991306744997845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13413337238272	7.928483866804442
2	11.697313152404758	2.5713751390075688
3	4.142895258241735	1.3660749778719592
4	1.9250274093557038	0.8463426699502831
5	1.0841465690310996	0.5958093748378899
6	0.7569823465531305	0.4992135102908231
7	0.5483234409434291	0.4218753794417358
8	0.4290623691807467	0.3772764889920877
9	0.3440122125159647	0.34030293766095193
>10	3.492002128330397	8.956121816444163
>50	1.5940101530924904	12.848536563734811
>100	1.7121405406790093	32.33394184808732
>500	0.07667696577521338	5.721620063252397
>1k	0.05735187683999699	13.158708642735881
>5k	0.0034286448110867774	2.44556955969564
>10k+	0.002493559862608565	9.588747161192055
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	91472	2.190817020122886	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	60118	1.4398672557257706	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	36834	0.8821995159087633	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	34074	0.8160956264612912	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	21256	0.5090957514838647	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13202	0.31619693785707476	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	11750	0.28142054384340465	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	11181	0.26779260431600915	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	8762	0.20985589831114143	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT	7706	0.18456397539210864	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	7652	0.1832706384246581	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	7068	0.16928343862852632	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	6456	0.15462561966408686	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	6331	0.1516317840912847	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	5845	0.1399917513842298	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT	5817	0.13932113221592213	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	5540	0.1326867925865925	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	5115	0.12250775163906509	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	5092	0.12195688589366951	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	4890	0.11711884760802119	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	4844	0.11601711611723	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	4843	0.11599316543264757	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGT	4797	0.11489143394185636	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA	4635	0.11101142303950473	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	4429	0.10607758201552674	No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT	4416	0.10576622311595534	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	4354	0.10428128067184543	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4287	0.10267658480482347	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT	4227	0.10123954372987844	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	4196	0.10049707250782348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.042823824033362344	0.0	0.0	0.0	0.0
2	0.0796120755519555	0.0	0.0	0.0	0.0
3	0.09925163690953778	0.0	0.0	0.0	0.0
4	0.12171737904784533	0.0	0.0	0.0	0.0
5	0.14073422260628476	0.0	0.0	0.0	0.0
6	0.17000195916599883	0.0	0.0	0.0	0.0
7	0.18952176710066904	0.0	0.0	0.0	0.0
8	0.2154843091880095	0.0	0.0	0.0	0.0
9	0.24094388689911925	0.0	0.0	0.0	0.0
10	0.2611103633175147	0.0	0.0	0.0	0.0
11	0.2815642479508992	0.0	0.0	0.0	0.0
12	0.30810160646821766	0.0	0.0	0.0	0.0
13	0.3299685814919648	0.0	0.0	0.0	0.0
14	0.3501829592795251	0.0	0.0	0.0	0.0
15	0.37789390134138207	0.0	0.0	0.0	2.395068458241742E-5
16	0.4015811283933929	0.0	0.0	0.0	2.395068458241742E-5
17	0.43235775808179927	0.0	0.0	0.0	2.395068458241742E-5
18	0.4650025411676342	0.0	0.0	0.0	2.395068458241742E-5
19	0.48737248056761207	0.0	0.0	0.0	2.395068458241742E-5
20	0.5110118062504581	0.0	0.0	0.0	2.395068458241742E-5
21	0.5344355757720622	0.0	0.0	0.0	2.395068458241742E-5
22	0.5551050165666885	0.0	0.0	0.0	2.395068458241742E-5
23	0.5813310161844356	0.0	0.0	0.0	2.395068458241742E-5
24	0.6048026870752047	0.0	0.0	0.0	2.395068458241742E-5
25	0.6269331196293584	0.0	0.0	0.0	2.395068458241742E-5
26	0.6550751740136989	0.0	0.0	0.0	2.395068458241742E-5
27	0.6791695627036107	0.0	0.0	0.0	2.395068458241742E-5
28	0.7071200116112919	0.0	0.0	0.0	2.395068458241742E-5
29	0.7363877481710059	0.0	0.0	0.0	2.395068458241742E-5
30	0.7653920272003135	0.0	0.0	0.0	2.395068458241742E-5
31	0.7919533364022144	0.0	0.0	0.0	2.395068458241742E-5
32	0.8214126784385878	0.0	0.0	0.0	2.395068458241742E-5
33	0.8484769520167195	0.0	0.0	0.0	2.395068458241742E-5
34	0.8742239379428183	0.0	0.0	0.0	2.395068458241742E-5
35	0.8970728910344444	0.0	0.0	0.0	2.395068458241742E-5
36	0.9183171482590486	0.0	0.0	0.0	2.395068458241742E-5
37	0.9370705342870815	0.0	0.0	0.0	2.395068458241742E-5
38	0.9491177286320375	0.0	0.0	0.0	2.395068458241742E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	5530	0.0	164.98178	44
GCCGTTA	20	6.710041E-6	115.138214	43
AATCGCA	660	0.0	112.700935	44
ACATTGG	1175	0.0	103.8587	44
TGTTTTT	2800	0.0	97.95926	44
GACACGA	65	6.63078E-7	89.40218	44
TATACCG	65	6.63078E-7	89.40218	44
ACCACTC	655	0.0	86.94533	44
CCTATCA	5620	0.0	81.53916	43
ACTTCTA	3490	0.0	80.59005	44
CCTTAGC	14670	0.0	76.75882	43
ACCTTAG	11010	0.0	73.14671	42
CCCATAC	1760	0.0	72.639275	44
CAGGCAC	2175	0.0	72.13832	44
CCCTATC	6940	0.0	72.13545	42
GTCGCAA	90	0.0	71.65791	42
TAATTCC	900	0.0	71.02507	44
GATGCGC	70	0.0	69.098694	42
CTAATTC	460	0.0	68.832634	43
TCGAACA	55	0.0032952446	63.394276	44
>>END_MODULE