FastQCFastQC Report
Tue 24 May 2016
ERR841000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761472
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG150710.8555912327871235No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT142290.8077903026559604No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC102040.5792882316607928No Hit
AAGCAGTGGTATCAACGCAGAGTG93680.53182792573484No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT90720.5150237982778041No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTGCA85920.48777386186098903No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG83150.47204837772045194No Hit
AAGCAGTGGTATCAACGCAGAGTGCA74760.42441775969189405No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA60700.3445981542709734No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA48800.27704102023761945No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT42890.24348953602441595No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTG41580.2360525742106602No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC41130.23349789267158375No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT40730.23122706463684917No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCAC38610.21919167605275588No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA31150.17684073320495586No Hit
AAGCAGTGGTATCAACGCAGAGTGC30020.17042564400683066No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTT29390.16684908985212368No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29040.16486211532173092No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCT25220.14317570759001563No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTT24050.13653353558841697No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCC21880.12421429349998184No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC21810.12381689859390328No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG21620.12273825527740435No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA19830.11257629982196707No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTC18350.10417423609344911No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC17850.10133570105003088Illumina Single End Adapter 2 (100% over 25bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG100.0027412241199.2134944
AGCGACT352.0736479E-10170.7544144
ATGCGCA405.275069E-10149.4101144
TCAACCG201.0909974E-4149.4101144
CACTGCA23200.0103.04146644
CGAGCAA608.8475645E-999.6067544
CTACGGG251.8022154E-594.36391443
CTATCAT10550.093.4698344
GAAACGA901.6370905E-1188.5393344
CTATTGC601.0301956E-683.0056244
AATCCGC150.001215671481.5148242
CCTATCG550.081.5148242
CCACGAC150.001215671481.5148242
CTATCGA800.081.09399443
TACGGGC504.1674284E-579.68539444
AGCGCCT1004.1836756E-1179.68539444
TTAGAGT3800.076.0156744
CGTCGAG400.001717823874.70505544
CGTGAAC659.822543E-1172.5876343
ACCTCGA556.683723E-572.4412744
Produced by FastQC (version 0.11.5)