##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841000.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1761472
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.136265577880316	39.0	37.0	39.0	34.0	39.0
2	37.727800952839445	39.0	37.0	39.0	35.0	39.0
3	37.926320713585	39.0	38.0	39.0	35.0	39.0
4	38.36385874995458	39.0	38.0	40.0	35.0	41.0
5	39.17766220524652	40.0	38.0	41.0	35.0	41.0
6	39.14460178759583	40.0	38.0	41.0	35.0	41.0
7	38.77819460087927	40.0	38.0	41.0	35.0	41.0
8	38.96183021927116	40.0	38.0	41.0	35.0	41.0
9	39.06520512389638	40.0	38.0	41.0	35.0	41.0
10	38.53487310612942	40.0	38.0	41.0	34.0	41.0
11	38.79381449151619	40.0	38.0	41.0	35.0	41.0
12	38.82659843585365	40.0	38.0	41.0	35.0	41.0
13	38.803470620026886	40.0	38.0	41.0	35.0	41.0
14	38.814723140646	40.0	38.0	41.0	35.0	41.0
15	38.8790142562584	40.0	38.0	41.0	35.0	41.0
16	38.66653741870436	40.0	38.0	41.0	35.0	41.0
17	38.58038958325764	40.0	38.0	41.0	35.0	41.0
18	38.60734033808088	40.0	38.0	41.0	35.0	41.0
19	38.552148998110674	40.0	38.0	41.0	35.0	41.0
20	38.515787364204485	40.0	38.0	41.0	35.0	41.0
21	38.29716339516041	40.0	37.0	41.0	34.0	41.0
22	38.272091750535914	40.0	37.0	41.0	34.0	41.0
23	37.71803695999709	39.0	36.0	41.0	33.0	41.0
24	37.77803621062384	39.0	36.0	41.0	33.0	41.0
25	37.77975610386403	39.0	36.0	41.0	33.0	41.0
26	37.77271864163439	39.0	36.0	41.0	33.0	41.0
27	37.666504540697716	39.0	36.0	41.0	33.0	41.0
28	37.37694319173222	39.0	35.0	41.0	33.0	41.0
29	37.38434306078944	39.0	35.0	41.0	32.0	41.0
30	37.40277711361165	39.0	35.0	41.0	33.0	41.0
31	37.38852679216185	39.0	35.0	41.0	33.0	41.0
32	37.33711292304453	39.0	35.0	41.0	32.0	41.0
33	37.346129936208264	39.0	35.0	40.0	33.0	41.0
34	37.245658102159496	38.0	35.0	40.0	32.0	41.0
35	37.17046880935606	38.0	35.0	40.0	32.0	41.0
36	37.1273242917428	38.0	35.0	40.0	32.0	41.0
37	37.12949413747754	38.0	35.0	40.0	32.0	41.0
38	36.98903510086628	38.0	35.0	40.0	32.0	41.0
39	36.85990776726409	38.0	35.0	40.0	31.0	41.0
40	36.67210377757532	38.0	35.0	40.0	31.0	41.0
41	36.57106039881938	38.0	35.0	40.0	31.0	41.0
42	36.38228591976022	37.0	34.0	40.0	31.0	41.0
43	36.28043647591313	37.0	34.0	40.0	31.0	41.0
44	36.08709118851946	37.0	34.0	40.0	30.0	41.0
45	35.86961431569267	37.0	34.0	40.0	29.0	41.0
46	35.52166721604822	37.0	33.0	40.0	28.0	41.0
47	35.50294615549618	36.0	34.0	40.0	29.0	41.0
48	36.326832515005336	37.0	35.0	40.0	31.0	41.0
49	36.964098847516965	38.0	35.0	40.0	32.0	41.0
50	36.63257241047811	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	18.0
22	59.0
23	107.0
24	216.0
25	413.0
26	734.0
27	1574.0
28	3345.0
29	7054.0
30	14164.0
31	26138.0
32	44721.0
33	72742.0
34	108157.0
35	109656.0
36	173842.0
37	282250.0
38	293866.0
39	288722.0
40	333686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.69878147367656	16.0128006576318	30.288417868691642
2	22.09646250408749	31.376485121534714	28.61725874722959	17.909793627148204
3	31.043865585146968	17.63280937761145	24.492640246339427	26.830684790902154
4	16.60531646259492	21.363212131671695	29.424992279184682	32.606479126548706
5	15.39138856592668	33.663265723213314	26.68257003233659	24.262775678523415
6	29.602741343603533	24.96639174508593	24.02723404062057	21.403632870689968
7	23.813549122552047	25.193871943465467	29.650882897939905	21.34169603604258
8	32.12477972968063	22.784807252116412	25.177465210914505	19.91294780728845
9	33.041796860807324	24.09916251862079	22.431977346219526	20.42706327435236
10	23.49864204483523	21.328298150637647	33.75245249427751	21.420607310249608
11	23.404345910692875	32.57746929840497	23.91726919303855	20.100915597863604
12	21.1997125131708	23.070080023979944	33.62176634087854	22.108441121970714
13	21.053073792827817	21.7981892417251	22.745805780619847	34.40293118482723
14	17.997901754895906	34.990110543908735	22.567829633397523	24.44415806779784
15	19.050430548995386	38.35536414998365	21.38836155215638	21.205843748864588
16	19.42806925117175	31.07122906296552	18.867912745703595	30.632788940159138
17	30.09227509719144	29.798032554590705	19.90074210660175	20.2089502416161
18	19.11980434545653	25.381044944228464	21.14810794608146	34.351042764233554
19	21.587115775896525	34.74281737092613	21.027016041129237	22.643050812048106
20	31.76553473458562	22.890173672928096	22.554261435889984	22.790030156596302
21	21.339936144315665	36.76288921992516	21.22026350688515	20.676911128874032
22	32.36832603640592	25.439689078225484	20.58982487374196	21.60216001162664
23	22.858609163245287	23.965126893870583	32.21317171093268	20.963092231951457
24	31.604589797623806	25.313487810195113	22.001485121534717	21.080437270646367
25	21.928829648857327	26.191585095172325	22.09456646270204	29.785018793268307
26	23.36916815737219	36.46034144392921	20.646589902568646	19.523900496129958
27	30.839402814553647	26.546283745027942	21.775507624017585	20.838805816400825
28	22.739018087855296	25.901685002088847	28.180550050763113	23.178746859292744
29	26.529420238451195	27.940238305928837	23.87961955304625	21.650721902573718
30	23.756711654215085	27.754031284064055	23.630785755537	24.85847130618386
31	22.752340095296006	27.78789169206509	27.77574259788544	21.684025614753462
32	20.37542453398195	27.305218733903647	26.97158088983962	25.34777584227479
33	21.092843912687254	26.567775131743797	30.304676466556362	22.034704489012583
34	21.567019421372336	28.63175539648989	24.44192625070945	25.359298931428327
35	22.020240719756618	25.151480715470807	26.61151389804314	26.216764666729436
36	20.996315239220863	29.38521294611644	26.12887611516812	23.489595699494576
37	21.450666694892156	30.48319833610737	26.49162893712507	21.574506031875398
38	27.273696165515954	27.460474472854195	22.52690267078325	22.738926690846597
39	24.601484211120034	30.53114399211429	22.27021720621295	22.597154590552726
40	22.013079976573177	30.02124411617969	24.542580421248996	23.423095485998136
41	24.40915837935491	30.03794851753962	23.21276880199924	22.340124301106233
42	20.63998381541817	33.16703028858578	24.515415190643427	21.67757070535262
43	23.969685274200906	28.040419634398795	24.81239254762991	23.177502543770395
44	20.55114970791127	28.21811200659696	23.964160523175618	27.266577762316153
45	21.09291120162229	29.429982441232223	27.44509785801065	22.032008499134832
46	21.582117966794932	27.979778274089824	25.57802787718968	24.86007588192557
47	21.86962796163905	29.447254702720777	27.09355289937754	21.589564436262638
48	26.279740317779726	26.071105513527204	23.54263331536955	24.106520853323516
49	20.59317504492833	27.917141606834296	24.250228749634605	27.23945459860277
50	20.106554144875645	32.82972923964321	25.597748029093715	21.465968586387437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34843.0
1	29752.0
2	21594.5
3	17417.5
4	14788.0
5	10643.0
6	6215.5
7	4717.5
8	5149.5
9	5153.0
10	5040.5
11	5501.0
12	6321.0
13	6967.0
14	7210.5
15	7069.5
16	6830.5
17	6435.0
18	6170.5
19	6580.0
20	7271.0
21	8470.0
22	9480.5
23	10028.5
24	11012.0
25	12979.5
26	16224.0
27	19559.0
28	21399.0
29	22947.0
30	26238.5
31	31855.5
32	39411.5
33	44436.0
34	47432.0
35	50573.5
36	53993.5
37	58791.5
38	63491.5
39	68935.5
40	73683.5
41	80136.5
42	87763.0
43	88788.5
44	89519.5
45	94619.0
46	102342.5
47	111747.5
48	141247.0
49	173764.5
50	214050.5
51	256085.0
52	245558.0
53	206936.5
54	179044.0
55	165031.0
56	149183.5
57	127228.5
58	109913.5
59	93399.5
60	77628.5
61	66713.0
62	56307.0
63	46172.0
64	37640.0
65	31139.0
66	22309.0
67	15028.5
68	11865.5
69	9170.0
70	7177.5
71	5350.0
72	3741.5
73	2603.0
74	1690.5
75	1097.0
76	766.0
77	467.0
78	292.0
79	231.0
80	172.5
81	122.0
82	81.0
83	53.0
84	40.0
85	27.5
86	17.0
87	12.0
88	7.0
89	5.5
90	4.0
91	2.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	20157.0
25	13945.0
26	18822.0
27	28194.0
28	28192.0
29	21044.0
30	17829.0
31	27138.0
32	17766.0
33	16126.0
34	12294.0
35	15307.0
36	13022.0
37	10391.0
38	11969.0
39	14044.0
40	16677.0
41	15206.0
42	18299.0
43	28043.0
44	66048.0
45	141743.0
46	196227.0
47	156120.0
48	258726.0
49	230714.0
50	347429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.724860263754529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.51790663585315	9.678484146695727
2	11.33881506534992	3.112473046569647
3	4.122402370269982	1.697381894487779
4	2.1372693065495336	1.1733489031361557
5	1.2941153583161236	0.888077622903371
6	0.8815190902108784	0.7259235799785798
7	0.6968207062803983	0.6694636775812444
8	0.5539647343299595	0.6082470855781277
9	0.4248082561918026	0.5247390559609554
>10	4.644091657962921	15.30480419743116
>50	2.011823421978067	19.770213367106642
>100	1.2995182378850907	29.44410773597997
>500	0.049496885792022154	4.767469452072317
>1k	0.023398527828955927	6.1760455863650945
>5k	0.002699830134110299	3.019533187213997
>10k+	0.0013499150670551496	2.4396874609392296
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGCAG	15071	0.8555912327871235	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	14229	0.8077903026559604	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	10204	0.5792882316607928	No Hit
AAGCAGTGGTATCAACGCAGAGTG	9368	0.53182792573484	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT	9072	0.5150237982778041	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTGCA	8592	0.48777386186098903	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	8315	0.47204837772045194	No Hit
AAGCAGTGGTATCAACGCAGAGTGCA	7476	0.42441775969189405	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6070	0.3445981542709734	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	4880	0.27704102023761945	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4289	0.24348953602441595	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTG	4158	0.2360525742106602	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	4113	0.23349789267158375	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	4073	0.23122706463684917	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCAC	3861	0.21919167605275588	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3115	0.17684073320495586	No Hit
AAGCAGTGGTATCAACGCAGAGTGC	3002	0.17042564400683066	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTT	2939	0.16684908985212368	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2904	0.16486211532173092	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCT	2522	0.14317570759001563	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTT	2405	0.13653353558841697	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCC	2188	0.12421429349998184	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	2181	0.12381689859390328	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2162	0.12273825527740435	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1983	0.11257629982196707	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTC	1835	0.10417423609344911	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC	1785	0.10133570105003088	Illumina Single End Adapter 2 (100% over 25bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12438460560258693	0.0	0.0	0.0	0.0
2	0.27477019220288484	0.0	0.0	0.0	0.0
3	0.30548314137267013	0.0	0.0	0.0	0.0
4	0.3564064600515932	0.0	0.0	0.0	0.0
5	0.66478490716855	0.0	0.0	0.0	0.0
6	0.7942221051484213	0.0	0.0	0.0	0.0
7	0.8618927805835119	0.0	0.0	0.0	0.0
8	0.9468785197834538	0.0	0.0	0.0	0.0
9	0.9997888129927697	0.0	0.0	0.0	0.0
10	1.0440131889692257	0.0	0.0	0.0	0.0
11	1.1266713294335646	0.0	0.0	0.0	0.0
12	1.1719175780256512	0.0	0.0	0.0	0.0
13	1.2147226864803982	0.0	0.0	0.0	0.0
14	1.2663840242706101	0.0	0.0	0.0	0.0
15	1.3601692221051485	0.0	0.0	0.0	0.0
16	1.4076295280311013	0.0	0.0	0.0	0.0
17	1.6331795225811139	0.0	0.0	0.0	0.0
18	1.6857491915852196	0.0	0.0	0.0	0.0
19	1.9732927914834866	0.0	0.0	0.0	0.0
20	2.014451549613051	0.0	0.0	0.0	0.0
21	2.120555989536024	0.0	0.0	0.0	0.0
22	2.1530856011335975	0.0	0.0	0.0	0.0
23	2.2088344293863313	0.0	0.0	0.0	0.0
24	2.2616311811939105	0.0	0.0	0.0	0.0
25	2.3033576463321586	0.0	0.0	0.0	0.0
26	2.6031069469171237	0.0	0.0	0.0	0.0
27	2.644038622243215	0.0	0.0	0.0	0.0
28	2.721303546125059	0.0	0.0	0.0	0.0
29	2.7924940050139884	0.0	0.0	0.0	0.0
30	2.840862642153835	0.0	0.0	0.0	0.0
31	2.872938088144461	0.0	0.0	0.0	0.0
32	2.904729680630745	0.0	0.0	0.0	0.0
33	2.936464502416161	0.0	0.0	0.0	0.0
34	2.9609326744904263	0.0	0.0	0.0	0.0
35	2.9871039675907425	0.0	0.0	0.0	0.0
36	3.011004432656324	0.0	0.0	0.0	0.0
37	3.036891872252298	0.0	0.0	0.0	0.0
38	3.0505168404607055	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTACG	10	0.0027412241	199.21349	44
AGCGACT	35	2.0736479E-10	170.75441	44
ATGCGCA	40	5.275069E-10	149.41011	44
TCAACCG	20	1.0909974E-4	149.41011	44
CACTGCA	2320	0.0	103.041466	44
CGAGCAA	60	8.8475645E-9	99.60675	44
CTACGGG	25	1.8022154E-5	94.363914	43
CTATCAT	1055	0.0	93.46983	44
GAAACGA	90	1.6370905E-11	88.53933	44
CTATTGC	60	1.0301956E-6	83.00562	44
AATCCGC	15	0.0012156714	81.51482	42
CCTATCG	55	0.0	81.51482	42
CCACGAC	15	0.0012156714	81.51482	42
CTATCGA	80	0.0	81.093994	43
TACGGGC	50	4.1674284E-5	79.685394	44
AGCGCCT	100	4.1836756E-11	79.685394	44
TTAGAGT	380	0.0	76.01567	44
CGTCGAG	40	0.0017178238	74.705055	44
CGTGAAC	65	9.822543E-11	72.58763	43
ACCTCGA	55	6.683723E-5	72.44127	44
>>END_MODULE