FastQCFastQC Report
Tue 24 May 2016
ERR840999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217651
Sequences flagged as poor quality0
Sequence length24-50
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAA79403.648042048968302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA78883.6241505897055375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA67433.0980790347850458No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA66633.0613229436115614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52792.4254425663102857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45982.1125563401960017No Hit
AAAAAAAAAAAAAAAAAAAAAAAA40371.8548042508419442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28631.3154086128710643No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC21190.9735769649576617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14720.6763120775921084No Hit
TTATTATGACACAAGGAAGTCTCGACTTGAATTGCAAAAAGATGTTAG13620.6257724522285677No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG12480.5733950223063529No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA11540.5302066151775089No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA11230.5159636298477839No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9550.4387758383834671No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA9480.4355596804057872No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA9310.4277490110314219No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA9150.420397792796725No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA8880.40799261202567416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.36985816743318434No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA7570.3478045127290938No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.3450478058910825No Hit
TTATTATGACACAAGGAAGTCTCGACTTGAATTGCAAAAAGATGTT7410.340453294494397No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA6720.3087511658572669No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6660.3059944590192556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG6280.28853531571185065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG5860.26923836784577143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG5840.2683194655664343No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGC5800.2664816610077601No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.2549953825160463No Hit
TTATTATGACACAAGGAAGTCTCGACTTGAATTGCAAAAAGATG5230.24029294604665266No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA5210.23937404376731555No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA5050.23202282553261874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4990.22926611869460742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4780.2196176447615678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG4460.2049152082921742No Hit
CAAAAAAAAAAAAAAAAAAAAAAA4260.19572618549880313No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCGAACATCTACAC4150.19067222296244907No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.18423990700708934No Hit
TAAAAAAAAAAAAAAAAAAAAAAA3790.17413198193438117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.17367253079471262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.16724021483935292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG3520.1617268011633303No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG3490.16034844774432463Illumina Single End Adapter 1 (100% over 32bp)
ATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGC3480.15988899660465608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG3220.14794326697327373No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.13829479304023415No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.1378353419005656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.13737589076089704No Hit
CACCACACAAGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAGCCC2960.13599753734189138No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.1327813793642115No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.13232192822454295No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2760.12680851454852032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2740.12588961226918324No Hit
AAAAAAAAAAAAAAAAAAAAAAAG2650.12175455201216627No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA2580.11853839403448639No Hit
CGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGA2430.11164662693945812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.11118717579978957No Hit
AATTCGTGGAGAAAGAAATGGCTC2390.1098088223807839No Hit
TCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATG2380.10934937124111535No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCCC2340.10751156668244116No Hit
TATCAACTCAGAGTGCAGTGCTTTAACTGGGGGCTGAGGCAGGAG2290.10521431098409839No Hit
CACCACACAAGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAA2270.10429540870476128No Hit
CATCTACACCACACAAGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGG2260.10383595756509273No Hit
TCAAGTGACTAAGAGCATGTGGTGGATGCCTTGGCGATGATAGGCGA2240.10291705528575565No Hit
ATCAGCAAACATCTTCATCACAAGTCATACTTGAGTGAAGATGAGAGCG2230.1024576041460871No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.10107925072708143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.10061979958741288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA157.159544E-6294.9315544
GAAGCCC400.0294.9315244
GGGCCGC108.419622E-4294.9315244
ACTTACT351.2732926E-11252.7984644
GAACACA450.0229.3911944
AGTATCG403.274181E-11221.1986544
ATCCCAT150.002834453196.6210344
ATATTCA150.002834453196.6210344
GCCCTAT301.2187229E-10183.6653743
CAATGAA100.00348385183.6653743
GATAAGC450.0183.6653743
TATCCCA100.00348385183.6653743
TGGGCCG100.00348385183.6653743
CTATCAT255.4947115E-5176.9589244
TGAACAC405.456968E-12160.707243
ATTTAAA401.9201361E-6147.4657644
GGAAGCC500.0146.932343
GTCCACA201.497762E-4137.7490243
TGCCCTA301.7935236E-9125.0281542
CGATAAG450.0125.02814542
Produced by FastQC (version 0.11.5)